Analysis on the Genetic Diversity of Six Bactrian Camel Populations in China Using Microsatellite Marker     

BAI Li  ZHOU Jun-wen  LI Xin-hai  FENG Deng-zhen 《中国畜牧兽医》2017,44(6):1734-1745

In order to understand the genetic diversity of Bactrian camel and the genetic evolutionary relationships between different populations,the genetic diversity of Alashan camel, Qinghai camel, Nanjiang camel, Beijiang camel, Subei camel and Sunite camel populations in China were analyzed using 10 microsatellite markers. By calculating heterozygosity (H), polymorphism information content (PIC), effective allele (Ne), Shannon information index, the genetic variation within populations were analyzed. By calculating the F-statistics, gene flow, genetic differentiation coefficient and genetic distance to analyze the genetic relationship between populations. The results showed that 89 alleles were tested at the 10 microsatellite loci,8.9 alleles were tested at every locus in average. All loci were medium-highly polymorphic loci (except YWLL08),the average PIC of the Bactrian camel population was between 0.488 to 0.752. The observed heterozygosity (0.355 to 0.448) of 6 Bactrian camel populations was lower than the expected heterozygosity (0.643 to 0.703). Almost all loci Shannon index was greater than 1,and most of loci were in Hardy-Weinberg disequilibrium. The genetic differentiation coefficient between groups (Fst) value was 0.059 and in the low degree of moderately differentiated state. The average Fis values of 6 Bactrian camel populations were positive which suggested 6 Bactrian camel populations had different levels of inbreeding. The standard genetic distance (D_S) and genetic distance T (D_A) clustering analysis showed that the Nanjiang camel and Beijiang camel jointed as one group, the Alxa camel, Qinghai camel, Subei camel, Sunite camel jointed as the other group. Research showed that Chinese Bactrian camel was abundant in genetic diversity, genetic variation within population was larger, and there was a phenomenon of inbreeding. There was a certain gene flow between populations, the differentiation of populations were mainly caused by genetic variation within population. 6 Chinese Bactrian camel populations were divided into two groups.  相似文献   

Distribution Difference of Microsatellite in 7 Domestic Animals Genomes     

WANG Yue-yue  LIU Xue-xue  DONG Kun-zhe  CHEN Xiao-fei  YE Shao-hui  MA Yue-hui 《中国畜牧兽医》2015,42(9):2418-2426

To investigate whether difference in SSRs distribution of 7 domestic animals genomes, the microsatellite sequence were searched by using MSDB (Microsatellite Search and Building Database) and analyzed in pig, horse, cow, goat, sheep, chicken and dog genomes.Preliminary results showed that SRRs number, frequency and density were found to be largely variable among these animals.The most abundant distribution was observed in dog genome with 1 436 242 identified SSRs loci.The minimum SSRs (276 564) number was found in chicken genome.Yet, the minimum SSRs (430 760) frequency and density were found in horse genome.Among these SSRs, mononucleotide repeat type motif was the most abundant and pentanucleotide repeat type motif was the rarest in all animals.Meanwhile, all the dominant SSRs for different repeat types were abundant in nucleotide A and T.In addition, there was variability among 7 animals of SSRs distribution.In general, SSRs distribution was the most similar in cow, goat and sheep genomes, and chicken was the most different from other animals.The results also showed that the length of microsatellite ranged mainly from 12 to 20 bp in all animals, which might be due to selection pressure of convergence.In short, these results revealed difference as well as conservation in SSRs distribution among different domestic animals genomes.In particular, genetically close animals tended to have similar SSR distribution patters.This would provide a basis for subsequent research of SSRs function.  相似文献   

鸡F_2群体的蛋品质性状与4号染色体微卫星标记的关联分析     

魏霞  杨柳  盖昊  邓学梅 《中国畜牧杂志》2010,46(19)

从鸡的4号染色体上选取6个与蛋品质性状相关的微卫星标记,对以中国泰和丝羽乌骨鸡与农大褐为亲本建立的CAU-BC F_2资源群体373只鸡进行了遗传检测,计算出这些微卫星标记座位的等位基因频率、多态信息含量、群体杂合度。通过统计软件,对各蛋品质性状与各标记间进行了关联分析,筛选出了与蛋重、蛋壳重、蛋黄颜色和蛋白重有关的分子遗传标记。结果表明:标记ADL0317与蛋重、蛋黄颜色和蛋白重呈极显著相关(P<0.01);标记LEI0094和标记LEI0063分别与蛋壳重和蛋黄颜色呈极显著相关(P<0.01)。本研究结果为蛋重、蛋壳重、蛋黄颜色和蛋白重的QTL定位和标记辅助选择提供理论依据。  相似文献   

俄罗斯蜜蜂保种群的遗传标识码:基因组微卫星标记谱图     

Brandorf A  Ivoilova M  李兴安  薛运波 《中国蜂业》2013,(Z3):17-22

俄罗斯蜜蜂是欧洲人大约在十九世纪中期向俄罗斯符拉迪沃斯托克及其周边海冰边疆(即俄罗斯远东地区)移居时带入的西方蜜蜂;欧洲黑蜂在欧洲向东方向的地理分布极限地域是位于俄罗斯西部平原(欧洲部分)与东部高原、山地(亚洲部分)之间的乌拉尔山脉,即俄罗斯中部地区。为了初步揭示俄罗斯蜜蜂与欧洲黑蜂之间的遗传关系,本研究通过聚合酶链反应体外扩增基因组微卫星标记的方法,对俄罗斯远东地区采样点的俄罗斯蜜蜂(俄罗斯当地人称之为俄罗斯远东黑蜂)和俄罗斯中部地区采样点的欧洲黑蜂(俄罗斯当地人称之为俄罗斯中部蜜蜂)进行了比对分析。聚丙烯酰胺凝胶电泳的结果显示,绝大多数蜜蜂样本在11个基因组微卫星位点均出现等位序列条带,两种蜜蜂样本的等位序列条带数构成了各自特有的基因组微卫星DNA等位序列标记谱图。这提示,俄罗斯蜜蜂样本的基因组微卫星DNA等位序列标记谱图作为俄罗斯蜜蜂保种群的遗传标识码,很可能具有特异性(相对于欧洲黑蜂样本的基因组微卫星DNA等位序列标记谱图)。  相似文献   

塔里木马鹿微卫星遗传多样性与产茸量的相关性研究   总被引：1，自引：0，他引：1  

袁国伟  席继峰  郭庆河  袁其彬  钟灵  彭林泽  曾献存  贾斌 《经济动物学报》2008,12(1):6-13

利用7个微卫星遗传标记,采用PCR扩增,12%非变性聚丙烯酰胺凝胶电泳、Sanguinetti银染法显色,对新疆塔里木马鹿亚种遗传多样性进行检测,统计各群体的等位基因组成、平均有效等位基因数(E)和平均基因纯合率(Rh),根据等位基因频率计算出各群体的平均遗传杂合度(h)、多态信息含量(PIC)和群体间的遗传距离,利用分子进化遗传分析软件(MEGA),采用邻结法(NJ)重建系统发生树。根据等位基因频率,利用PHYLIP(3.6)分析软件,采用最大似然法(ML)构建系统发生树,应用自举检验(bootstrap test)估计系统树中节点的自引导值(bootstrap value),并进行了系统发生分析。同时对部分马鹿群体个体基因型与产茸量之间进行了相关分析。结果表明:7个微卫星位点在3个塔里木马鹿中的多态信息含量除BMS2508和Celjp0023没有多态性,以及BM5004为中度多态外,其他4个微卫星均为高度多态,可作为有效的遗传标记用于3个塔里木马鹿群体遗传多样性和系统发生关系的分析。所有马鹿群体的平均PIC为(0.5196)、h(0.5552)和E(2.45),其基因多态性和遗传多样性相对丰富。塔里木马鹿35团群体与沙雅群体的血缘关系及遗传距离近于阿拉尔群体,3个塔里木马鹿群体的系统发育关系基本符合其地理分布和育成史。微卫星位点BM4208的166bp/185bp基因型和微卫星BM888的208bp/208bp基因型可以对塔里木马鹿35团群体高产茸量进行分子遗传标记。  相似文献   

马立克氏病毒感染致鸡胚成纤维细胞4号染色体上微卫星不稳定性研究     

尚月丽  乔丽平  韩涛  夏继飞  杨德吉 《南京农业大学学报》2009,32(3)

应用微卫星技术对感染马立克氏病毒5 d后的32个鸡胚成纤维细胞(CEF)4号染色体上的20个微卫星标记进行PCR扩增,扩增产物采用变性聚丙烯酰胺凝胶电泳,银染检测.试验结果显示:20个微卫星标记有18个表现出不同程度的微卫星不稳定性,其中ABR0622发生率最高,为37.50%;MCW0174、ADL0266和ABR0382的发生率较高,均为31.25%.在32组样品中有30个检测到微卫星不稳定性,其中11号样品发生率最高,为45.00%,其次是3号样品为35.00%.结论:CEF感染马立克氏病毒后出现微卫星不稳定性现象.  相似文献   

云南肉山羊黑色品系遗传结构的微卫星分析     

兰蓉  朱兰  伍红德  王鹏  胡钟仁  洪琼花 《中国草食动物》2012,(Z1)

应用微卫星技术对云南肉山羊黑色品系两个群体(石林群体和寻甸群体)、两个育种素材(云岭黑山羊和努比山羊)及对照群(萨能奶山羊)基因组DNA进行了11个微卫星位点多态性分析,以期为云南肉山羊黑色品系的选育提高及开发利用提供依据.结果表明:各群体平均期望杂合度(He)和平均多态信息含量(PIC)均在0.5以上,表明5个研究群体遗传多样性丰富,其中云南肉山羊黑色品系石林群体He和PIC值在5个群体中居最高,表明这个群体遗传多样性最为丰富;遗传分化的研究表明云南肉山羊黑色品系石林群体与寻甸群体之间存在着最大的基因流(Nm=18.981)、最高的遗传一致度(0.906)、最小的遗传分化系数(Fst=0.013)及最小的遗传距离(0.099),UPGMA聚类图也显示二者最后聚为一支,说明二者遗传结构基本一致,可归为同一类群体;UPGMA聚类图还显示云南肉山羊黑色品系两个群体(石林与寻甸)与育种素材努比山羊关系最近,与另一育种素材云岭黑山羊关系稍远,而与对照群萨能奶山羊的关系最远,分属于不同的两个分支.  相似文献   

福安水牛的分子遗传多样性研究   总被引：3，自引：0，他引：3  

刘庆华  叶鼎承  梁学武  鲍若虹  张瑞光 《经济动物学报》2008,12(3)

福安水牛是福建省地方优良品种之一,为了进一步阐明其群体遗传结构和遗传多样性,采用8对微卫星引物对福建福安、闽清、古田及南靖等4个福安水牛群体,共计130头样本进行检测分析。结果表明:福安水牛4个群体8个微卫星座位共有78个等位基因,其中4个群体共享的等位基因为50个,非共享等位基因28个。各等位基因频率介于0.015 2~0.527 8之间;共检测到基因型6~29种;4个群体的平均多态信息含量、杂合度、有效等位基因数分别为0.817 2±0.023 0、0.820 4±0.029 4和5.85±0.84;根据奈氏标准遗传距离进行UPGMA聚类分析,结果表明:遗传距离最远的是闽清群体与南靖群体(0.351 8),遗传距离最近的是福安群体与闽清群体(0.183 2)。  相似文献   

小麦品种沈免2135抗白粉病基因的微卫星标记     

戴启东  曹远银 《河南农业科学》2008,(5):27-29

对高抗白粉病的春小麦新品种沈免2135进行了微卫星标记,证明该品种对白粉病的抗性是由显性单基因控制。筛选到一个标记Xwmc376/420与抗病基因连锁,遗传距离为5.4 cM。  相似文献   

波尔山羊2号和8号染色体微卫星标记与产羔性状的关联研究   总被引：2，自引：0，他引：2  

徐云翔  陈世林  闻群英  沈忠  张春艳  姚红卫  滑国华  杨利国 《畜牧兽医学报》2008,39(8)

根据山羊2号和8号染色体遗传连锁图谱，选取8个微卫星标记INRA040、LSCV22、LSCV37、IDVGA64、FCB011、INRA129、SRCRSP10和CSSM47，分析它们与波尔山羊产羔性状的关联。结果表明：所研究的8个波尔山羊微卫星基因座均为高度多态性基因座（PIC〉0．5）。与波尔山羊产羔性状有显著效应的等位基因10个。其中与波尔山羊第1胎产羔数有显著正效应的等位基因1个：LSCV22的189bp；与波尔山羊第1胎产羔数有显著负效应的等位基因4个：LSCV22的179和191bp，SRCRSP10的273和303bp；与波尔山羊第2胎产羔数有显著正效应的等位基因6个：LSCV22的189bp、IDVGA64的281、283和245bp、FCB011的143和165bp。  相似文献