植物凝集素在抗病虫害中的应用     

崔兴国 《农业科技与装备》2011,(12)

植物凝集素是一类具有高度特异结合活性的蛋白,含有1个或多个可与单糖或寡聚糖可逆性特异结合的非催化结构区域。在简要介绍植物凝集素的定义、分类、特性的基础上,详细介绍从小麦胚芽、豌豆、半夏等植物中提取的植物凝集素在病虫害防治中的重要作用。  相似文献   

水稻根际高效解磷菌菌株的筛选     

祝凌云  马忠友  唐欣昀  常慧萍  韦兵  姚丽娟 《安徽农学通报》2008,14(15):43-44

以选择性培养基从水稻根际分离出81株解磷细菌,再利用FITC标记的稻胚凝集素从中筛选出21株能够和稻胚凝集素发生特异性作用的解磷菌株。将这些菌株在培养液中振荡培养7d,分别取10ml培养液进行消煮,测定其含磷量;选择解磷能力强的7个菌株在平板上进行水稻栽培试验,结果表明有2株解磷菌株对水稻生长有明显的促生效果,鲜重增产幅度超过45%,可作为研制微生物肥料的出发菌株。  相似文献   

新生仔猪红黄痢二联灭活疫苗的研制与应用Ⅰ——C型魏氏梭菌生物学特性研究   总被引：2，自引：0，他引：2  

刘泽文  熊忠良  杨克礼  梁望旺  伍锐  段正赢  李勇  徐涤平 《安徽农业科学》2008,36(21)

[目的]研究C型魏氏梭菌C59-2菌株的生物学特性。[方法]将该菌株接种于适宜培养基,进行毒素致死性试验和免疫原性试验。[结果]C型魏氏梭菌C59-2菌株在厌气肉肝汤和血液琼脂平板上生长良好,生化特性与魏氏梭菌一致;该菌在厌气肉肝汤中培养物的上清液0.0001ml静脉注射可致小鼠100%死亡,0.2ml静脉注射可使家兔全部死亡;免疫原性试验表明,该菌株制备成疫苗后免疫家兔,攻毒保护率可达75%。[结论]C型魏氏梭菌C59-2毒株适合于疫苗生产。  相似文献   

菊芋块茎发育期凝集素基因的鉴定和表达分析     

何腾飞  闻奋亮  岳杨  隆小华  高秀美  周兆胜 《分子植物育种》2021,19(4):1045-1054

凝集素是生物中普遍存在的一类糖结合蛋白,通过特异识别不同糖基而介导多种生物过程.本研究以'南菊芋1号'为研究材料,克隆并鉴定了12个凝集素基因.利用生物信息学分析这些基因编码蛋白的性质表明:等电点为4.96～5.23,偏酸性;依据其特异性糖基结合域归为三类:8个属于木菠萝家族(JRL),2个属于雪花莲家族(GNA),2...  相似文献   

裙带菜凝集素对鲫的免疫诱导   总被引：1，自引：0，他引：1  

张静  李丹彤  范庆华  邢殿楼  刘靖  姜川 《大连水产学院学报》2006,21(2):193-195

将从裙带菜Undaria pinnatifida中提取的凝集素作为免疫诱导制剂,注射到鲫Carassius auratusw体内,在连续注射7 d和9 d时,分别对鲫血清中的溶菌酶、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的酶活性进行测定,结果表明,注射凝集素7 d和9 d时,鲫血清中溶菌酶的酶活性比对照组分别提高18.83%、12.69%,SOD的酶活性比对照组分别提高66.67%、139.99%,CAT酶活性比对照组分别提高16.60%、63.63%。说明凝集素可以明显提高鲫血清中这3种酶的表达水平。  相似文献   

First Insights into the Repertoire of Secretory Lectins in Rotifers     

Marco Gerdol 《Marine drugs》2022,20(2)

Due to their high biodiversity and adaptation to a mutable and challenging environment, aquatic lophotrochozoan animals are regarded as a virtually unlimited source of bioactive molecules. Among these, lectins, i.e., proteins with remarkable carbohydrate-recognition properties involved in immunity, reproduction, self/nonself recognition and several other biological processes, are particularly attractive targets for biotechnological research. To date, lectin research in the Lophotrochozoa has been restricted to the most widespread phyla, which are the usual targets of comparative immunology studies, such as Mollusca and Annelida. Here we provide the first overview of the repertoire of the secretory lectin-like molecules encoded by the genomes of six target rotifer species: Brachionus calyciflorus, Brachionus plicatilis, Proales similis (class Monogononta), Adineta ricciae, Didymodactylos carnosus and Rotaria sordida (class Bdelloidea). Overall, while rotifer secretory lectins display a high molecular diversity and belong to nine different structural classes, their total number is significantly lower than for other groups of lophotrochozoans, with no evidence of lineage-specific expansion events. Considering the high evolutionary divergence between rotifers and the other major sister phyla, their widespread distribution in aquatic environments and the ease of their collection and rearing in laboratory conditions, these organisms may represent interesting targets for glycobiological studies, which may allow the identification of novel carbohydrate-binding proteins with peculiar biological properties.  相似文献   

Production of Lectins from Marine Algae: Current Status,Challenges, and Opportunities for Non-Destructive Extraction     

Intan Mariana Maliki  Mailin Misson  Peik Lin Teoh  Kenneth Francis Rodrigues  Wilson Thau Lym Yong 《Marine drugs》2022,20(2)

Marine algae are an excellent source of novel lectins. The isolation of lectins from marine algae expands the diversity in structure and carbohydrate specificities of lectins isolated from other sources. Marine algal lectins have been reported to have antiviral, antitumor, and antibacterial activity. Lectins are typically isolated from marine algae by grinding the algal tissue with liquid nitrogen and extracting with buffer and alcohol. While this method produces higher yields, it may not be sustainable for large-scale production, because a large amount of biomass is required to produce a minute amount of compound, and a significant amount of waste is generated during the extraction process. Therefore, non-destructive extraction using algal culture water could be used to ensure a continuous supply of lectins without exclusively disrupting the marine algae. This review discusses the traditional and recent advancements in algal lectin extraction methods over the last decade, as well as the steps required for large-scale production. The challenges and prospects of various extraction methods (destructive and non-destructive) are also discussed.  相似文献   

锯缘青蟹血清凝集素的特性和盐析提取研究     

郝珂  钱冬  刘问  潘清清 《淡水渔业》2008,38(4)

对锯缘青蟹(Scylla serrate)血清凝集素的凝集特性进行了研究。结果显示:锯缘青蟹血清凝集素对人、兔、鼠、鸽子、中华鳖、鲫红细胞均有凝集作用,其中对小鼠红细胞凝集活性最强,血凝活性可达29;凝集素活性最适pH 6.0~7.4;盐度对凝集活力有较大影响,NaC l浓度在0.4~0.5 mol/L时基本失活;未纯化血清凝集素对Ca2+、Mg2+未表现出依赖性;凝集素活性能被N-乙酰葡萄糖胺特异性抑制。对青蟹血清凝集素进行硫酸铵分级沉淀分离后,发现凝集素活性主要分布在25%饱和度硫酸铵沉淀区,该组分凝集比活比血清高27.44倍,SDS-PAGE结果表明主要蛋白带型为79 ku、75 ku、72 ku。  相似文献   

半乳糖结合凝集素的结构与功能   总被引：1，自引：0，他引：1  

李春华  魏晓锋  李祥瑞 《动物医学进展》2003,24(4):19-22

凝集素是自然界广泛存在的一大类糖结合蛋白 ,半乳糖结合凝集素 ( galectin)是凝集素家族中的一员 ,存在于多种生物、多种组织内 ,且进化保守 ,发挥着广泛的功能 ,参与细胞粘附、凋亡及免疫反应等多种生物学过程。目前 ,国内外对凝集素的研究日益增多 ,文章以哺乳动物的 galectin为重点 ,综述了 galectin的结构特性、配体、分泌方式和生物学功能等各方面的研究现状 ,为进一步了解和研究 galectin提供了参考  相似文献   

Sialic acid in external membrane of C-type primary sensory neurons with peripheral nerve injury increases excitability of the neurons     

LI Chen-xu  ZHANG Pei-jun  MA Guo-ying  LIU Ying  GUO Min-fang 《园艺学报》2016,32(5):943-946

AIM: To study the change of electrophysiological property of dorsal root ganglion (DRG) C-type primary sensory neurons with sialic acid on the membrane surface after rat sciatic nerve injury. METHODS: The operation to induce chronic constriction nerve injury (CCI) for establishing CCI pain model was performed in the rats, and normal rats served as controls. The thermal hyperalgesia behavior was observed to select CCI pain rats, and electrophysiological property of injured and normal C-type neurons was studied by intracellular recording. Ca²⁺ and neuraminidase (NA) were topically added on the extracellular membrane of C-type neurons to counteract or selectively remove the negatively charged sialic acid residues, and at the same time the change of electrophysiological property was observed. RESULTS: The rest potential (RP) of injured C-type neurons shifted to depolarizing direction. The incidence of evoked action potential (AP) was higher, and the rheobase to evoke AP was lower than the control. After topical application of Ca²⁺ and NA on injured C-type neurons, hyperpolarized RP and increased rheobase to evoke AP were observed, indicating the excitability of injured C-type neurons diminished. However, these treatments to normal neurons had no effect on electrophysiological property. CONCLUSION: Increased negative charge on the injured C-type neuron surface, carried by the sialic acid residues, contributes to the change of electrophysiological property.  相似文献