芳香化酶抑制剂来曲唑对胡子鲇性腺分化及相关基因表达的影响   总被引：2，自引：0，他引：2  

李广丽  邓思平  孙晶  王文达  师尚丽  朱春华 《中国水产科学》2013,20(5):911-917

采用组织学和荧光实时定量PCR方法, 检测饲喂不同剂量芳香化酶抑制剂来曲唑(letrozole, 50、100和200 μg/g)对胡子鲇(Clarias fuscus)性腺组织学和性别比率的影响, 以及200 μg/g 来曲唑对性分化前后脑型芳香化酶基因(Cyp19a1b)和翼状螺旋/叉头转录因子2(Foxl2)基因表达的影响, 结果表明, 50 μg/g 剂量的来曲唑对胡子鲇性腺分化无显著影响; 但100 μg/g和200 μg/g剂量的来曲唑可促进胡子鲇精巢分化, 抑制卵巢分化, 卵巢腔最早出现时间和初级卵母细胞出现时间均分别推迟3 d和6 d, 而初级精母细胞最早出现时间则分别提前2 d和5 d, 且雄性率分别达65.8%和71.3%, 显著高于50 μg/L组和对照组(P<0.05)。胡子鲇性腺分化前(出膜后12 d), Cyp19a1b和Foxl2基因即开始表达, 性腺分化前后Cyp19a1b相对表达量无显著差异(P>0.05), 但Foxl2相对表达量则随性分化而逐渐降低, 且来曲唑显著抑制性腺分化过程中Cyp19a1b和Foxl2的表达(P<0.05)。结果表明, 100 μg/g 以上剂量的来曲唑可有效诱导胡子鲇分化为雄性; Cyp19a1b可能不直接参与胡子鲇性腺分化, 但Foxl2直接参与此过程。  相似文献   

养殖密度对革胡子鲶生长性能及补体C3·C4的影响     

徐敏  王晓梅  季延滨  王存  戴伟  潘宝平 《安徽农业科学》2011,39(20):12272-12274

[目的]研究养殖密度对革胡子鲶生长性能及血清补体C3、C4的影响。[方法]以体重（30.71±0.89）g革胡子鲶为试验对象,测定4种养殖密度（35、65、95、125 kg/m3）下革胡子鲶生长性能及血清补体C3、C4的变化。[结果]养殖60 d后,随着养殖密度升高,革胡子鲶终末重、日增重均呈下降趋势,其中35 kg/m3与65 kg/m3密度组间差异不显著（P〉0.05）。各密度处理组间饵料系数均无显著差异（P〉0.05）。低密度处理组（35 kg/m3与65 kg/m3）与高密度处理组（95 kg/m3与125 kg/m3）间死亡率差异显著（P〈0.05）。养殖密度对补体C3、C4的影响不显著（P〉0.05）。[结论]65 kg/m3可推荐为革胡子鲶的最佳养殖生产密度。  相似文献   

Some Physical and Chemical Properties of Clarias gariepinus Dried with Solar Dryers and Solar Tents in Sokoto,Nigeria     

Abubakar Baba Misau  Joseph Kayode Ipinjolu  Ibrahim Magawata  Abubakar Yunusa 《农业科学与技术》2013,(11):857-869

A research was conducted to assess the effects of three different drying techniques, namely open sun drying （OSD）, solar dryers （SD） and solar tent dryers （STD）. Data on physical characteristics such as weight, total length and standard length were recorded and proximate composition of the fish sample Clarias gariepinus （Burchell, 1822） was carried out before, during and after drying to assess changes on physical characteristics and nutrient composition at different level. After drying, the samples were subjected to storage for eight weeks. The result obtained shows that there was no significant （P 〉 0.01） difference in the drying weight of the fish samples between the direct SD and the STD. However, for the total and standard length there was significant （P 〈 0.01） difference in the drying techniques, but no significant difference （P 〉 0.05） in the drying period. There was no significant （P 〉 0.05） difference on the physical parameters, storage period, proximate composition of both direct SD and STD and the drying period, respectively. Therefore, direct SD can be substituted with the STD without altering the quality of the product.  相似文献   

革胡子鲶成鱼网箱养殖技术   总被引：1，自引：0，他引：1  

祖恩普  刘飞 《湖北农业科学》2008,47(5):573-574

在小浪底水库进行的革胡子鲶成鱼网箱养殖试验获得成功.由于大水面的理化特点,培养出来的革胡子鲶肉质鲜美,食用价值高,而且推迟了销售时间,提高了销售价格,取得了显著的经济效益.  相似文献   

胡子鲶冬春囤养技术   总被引：1，自引：0，他引：1  

谢炎福 《安徽农业科学》2006,34(7):1362-1363

对胡子鲶在我国北方地区的越冬进行了研究,试验及推广情况表明:采用内建越冬水泥池,外面搭建保温棚的方法,在越冬池内营造适宜的越冬环境对胡子鲶进行越冬是可行的;并对越冬棚的构建、越冬期间的管理等进行了总结,为其他热带鱼类在我国北方地区的越冬提供了成功的借鉴经验。  相似文献   

Short Communication Apparent digestibility of various oilseed cakes/meals in African catfish diets     

Oyedapo A. Fagbenro 《Aquaculture International》1998,6(4):317-322

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Enrichment of African catfish with functional selenium originating from garlic     

Edward Schram  Zoyne Pedrero  Carmen Cámara  Jan W van der Heul  & Joop B Luten 《Aquaculture Research》2008,39(8):850-860

We wanted to create functional seafood with high concentrations of organic selenium (seleno-methyl-selenocysteine and γ-glutamyl-seleno-methyl-selenocysteine) with anti-carcinogenic properties for human consumers. Garlic containing high concentrations of these organic selenium compounds was used as a selenium source in five experimental feeds for African catfish (1.9, 2.8, 3.9, 5.1 and 8.5 mg kg⁻¹ Se); a sixth experimental feed was formulated without garlic (1.9 mg kg⁻¹ Se). The experimental feeds were fed to African catfish [initial mean (SD) weight 100.7 (2.7) g] for 43 days with three replicates per treatment. Whole fish fillets were sampled for total selenium analysis (start and end) and selenium speciation (end). We found a positive linear relationship between dietary and fillet concentrations for total selenium and selenomethionine. The dietary total selenium concentration of 8.5 mg kg⁻¹ resulted in a total selenium concentration of 0.9 mg kg⁻¹ in the fillet (wet tissue). The majority of the selenium compounds recovered in an extract made from the fillet consisted of selenomethionine, considered to be important from a nutritional point of view. Seleno-methyl-selenocysteine, one of the organic selenium species to which superior anti-carcinogenic properties are attributed, was detected in the fillet but could not be quantified.  相似文献   

Heat treatment affects protein quality and protease activity in decapsulated cysts of Artemia when used as starter food for larvae of African catfish Clarias gariepinus (Burchell)   总被引：1，自引：0，他引：1  

García-Ortega  Verreth  Van Hoornyck  & Segner 《Aquaculture Nutrition》2000,6(1):25-31

Decapsulated cysts of Artemia subjected to different heat treatments (40, 60, 80 and 96 °C) were fed to African catfish Clarias gariepinus larvae. Heated cysts, untreated cysts and live Artemia nauplii as control constituted the experimental diets. Protein denaturation and solubility, total alkaline protease and specific trypsin activities in the cyst diets were evaluated. The growth of catfish larvae and the proteolytic activity of larval samples during development were also determined. Heat treatment of cysts increased protein denaturation and decreased protein solubility. The protease activity in the cyst diets decreased with higher heating temperatures. The growth of catfish larvae differed according to the diet; higher fish growth was achieved with nauplii and cysts heated at 40 °C. The digestive enzyme activity in larval samples remained similar in all dietary treatments during larval development. The quality of food protein and the way this protein is processed might be more important for successful larval growth than exogenous enzyme supply.  相似文献   

Effects of red clover extract on growth performance and body composition of African catfish <Emphasis Type="Italic">Clarias gariepinus</Emphasis>     

Funda?TuranEmail author  Ihsan?Akyurt 《Fisheries Science》2005,71(3):618-620

ABSTRACT:   The present study was conducted to investigate the effects of the red clover extract on growth, body composition and survival of African catfish Clarias gariepinus . Three concentrations (25, 50 and 75 mg/kg) of red clover at dietary were used for 120 days. The final weights of red clover-treated groups were significantly different from each other and the control group. The best growth was observed in the group receiving the 75 mg/red clover kg diet. Fish fed with all levels of red clover had higher weight gains than the control. The highest values of the specific growth rate and the food conversion rate were 1.14 ± 0.01 and 2.26 ± 0.04, respectively, for the 75 mg/red clover kg diet group. During the red clover administration period, the survival ranged from 97.78% for the 50 p.p.m. group to 95.56% for the 25 and 75 p.p.m. groups. Protein contents of the dosage groups were significantly different from control ( P  < 0.05). The highest value of protein content (19.97%) was observed for the 75 mg/ red clover kg diet. Lipid and ash contents were significantly affected by the red clover levels in the diets. The highest lipid contents were in the 75 mg/red clover kg diet (4.67%), and the highest ash content was 1.39% for the 25 mg/red clover kg diet.  相似文献   

Oocyte maturation inClarias batrachus. III. Purification and characterization of maturation-inducing steroid     

S. Haider  N. V. Rao 《Fish physiology and biochemistry》1992,9(5-6):505-512

Maturation-inducing steroid (MIS) in the Indian female catfish,Clarias batrachus, was purified and characterized from the incubation medium in which fully grown but immature folliculated oocytes were incubated with salmon gonadotropin (SG-G100) for 36 h. Maturation-inducing (MI) activity of residues obtained at various steps of extraction and purification was assessed byin vitro germinal vesicle breakdown (GVBD) assay using folliculated oocytes ofC. batrachus. The post incubation medium was extracted with diethyl ether. The ether phase was partitioned using 50% methanol plus n-hexane. The methanol phase which had MI activity was fractionated into 7 fractions using reverse-phase high-performance liquid chromatography. Of these 7 fractions, fraction 3 was found to be active in having MI ability and identified as 17 ,20-dihydroxy-4-pregnen-3-one (17,20-diOHprog). The authenticity of 17,20-diOHprog as the major follicular mediator of gonadotropin-induced oocyte maturation was further confirmed by thin-layer chromatography (TLC) in which fraction 3 was run along with authentic 17,20-diOHprog standard. This investigation gives a direct evidence that 17,20-diOHprog is the major naturally occurring MIS in Indian female catfish,C. batrachus.  相似文献