Genetic diversity of walking catfish, Clarias macrocephalus, in Thailand and evidence of genetic introgression from introduced farmed C. gariepinus     

Uthairat Na-Nakorn  Wongpathom Kamonrat  Thawatchai Ngamsiri 《Aquaculture (Amsterdam, Netherlands)》2004,240(1-4):145-163

The Thai walking catfish, Clarias macrocephalus Günther, 1864, is economically important to Thailand. It occupies marshes and swamps that are severely endangered due to population expansion and natural populations are thought to be suffering from massive back-crossing with the C. macrocephalus×C. gariepinus hybrids. Therefore, a study on genetic diversity of this species is required to enable efficient conservation and management plans. In this study, 25 natural populations were collected throughout the country, 12 populations from provinces locate in the Chaophraya river basin in the center of the country, 5 from the Mekong river basin, 1 from the east and 7 from the south. One population of hatchery origin was obtained from the Department of Aquaculture, Kasetsart University in Bangkok.

Twelve isozymes and one protein system were analyzed. Among 18 loci resolved, 8 were polymorphic. The number of alleles per locus, average polymorphism and individual polymorphism were significantly higher in collections from the Chaophraya river basin than from the Mekong, east and south. The hatchery population also had relative high genetic variation. Six out of twenty-six populations differed significantly from Hardy-Weinberg equilibrium after Bonferroni correction. None of loci pairs showed significant linkage disequilibrium after Bonferroni correction. The F_st value across loci was highly significant from zero. A neighbor-joining tree reveals that populations from the south were genetically distinct from the remaining populations.

Alleles peculiar to the African catfish [C. gariepinus (Burchell, 1822)] genome were observed in 12 of the natural populations and the hatchery population. This is evidence of genetic introgression which has probably persisted for several generations, since there was no significant genotype disequilibrium between the macrocephalus and gariepinus alleles at three diagnostic loci.  相似文献   

Steatitis in wild sharptooth catfish, Clarias gariepinus (Burchell), in the Olifants and lower Letaba Rivers in the Kruger National Park, South Africa     

Huchzermeyer KD  Govender D  Pienaar DJ  Deacon AR 《Journal of fish diseases》2011,34(7):489-498

Large numbers of adult Nile crocodiles, Crocodylus niloticus (Laurenti), died from pansteatitis during autumn and winter 2008 in the lower Letaba and Olifants River gorge in the Kruger National Park, South Africa. Consequently, the health status of fish from these waters was investigated. The study presents the pathological findings in fish inhabiting these rivers within the boundaries of the Park. Changes typical of steatitis were diagnosed in many of the larger specimens of sharptooth catfish, Clarias gariepinus (Burchell), caught within the Olifants River gorge. These fish carried large amounts of mesenteric fat with characteristic small brown granulomata within the adipose tissue. Necrosis and inflammation of the adipose tissues, with characteristic ceroid accumulation within the resultant granulomata and the associated aggregation of ceroid‐containing macrophages, were demonstrated histologically and were typical of steatitis. Other changes included mild thickening and pallor of the gill tissues and swollen, orange, fatty livers. Focal hepatic lipidosis was demonstrated histologically, and special stains revealed storage of large amounts of iron in the livers. Blood smears revealed chromatin clumping in erythrocyte nuclei and nuclear and cell membrane irregularities. This is the first record of steatitis in wild‐caught C. gariepinus.  相似文献   

胡子鲶败血症病原菌的研究   总被引：2，自引：0，他引：2  

韦友传  覃志彪  韦祖炜 《广西农业生物科学》2002,21(2):111-112

由患细菌性败血症的胡子鲶苗种分离到6个菌株，经菌株致病力测试、生化鉴定和药物敏感试验，结果表明嗜水气单胞菌是胡子鲶苗种败血症的病原菌，该病原菌对卡那霉素、恶喹酸、呋喃唑酮、丁胺卡那霉素等敏感，而对磺胺等9种药物不敏感。  相似文献   

Genetic basis of pectoral fin deformities in the African catfish Clarias gariepinus (Burchell, 1822), Heterobranchus longifilis (Valenciennes 1840) and their hybrids     

P O Aluko  J I Awopetu  & A O Adeola 《Aquaculture Research》2001,32(1):21-27

Morphological aberrations of the pectoral fins in nine mating combinations involving Clarias gariepinus (Burchell, 1822), Heterobranchus longifilis (Valenciennes, 1840) and their hybrids were investigated to determine the level and genetic basis of occurrence. The highest mean percentage survival in a Clarias× Clarias group was 75%, whereas the least mean percentage survival was 2% in the same group. The least mean percentage survival (40.3%) in the remaining three groups occurred in the cross of female hybrid (right pectoral fin absent) × male C. gariepinus (right pectoral fin absent). A maximum of nine types of aberrations was observed in the four mating groups – double dorsal fin, curved posterior dorsal fin, spineless right pectoral fin, right pectoral fin absent, left pectoral fin absent, rudimentary pectoral fin, both pectoral fins absent, double anal fin and curved anterior dorsal fin. These nine aberration types were recorded in the Clarias× Clarias group, with a total frequency ranging from 7.14% to 75.00%. The least number of aberrations was observed in the hybrid ×Clarias group (double dorsal fin and both pectoral fins absent) with a frequency range of 1.47–5.55%. No aberration was observed in two crosses involving female hybrid (right or left pectoral fin absent) × female C. gariepinus (normal). The level of aberrations in some of these crosses indicates the involvement of genotype rather than the influence of environment.  相似文献   

不同养殖密度下革胡子鲶胃肠道的定量组织学研究     

季延滨  张明月  王妍  王晓梅  戴伟  王存  孙学亮 《安徽农业科学》2013,(12):5399-5401

[目的]为革胡子鲶科学的高密度养殖和可持续发展提供参考依据。[方法]研究4种养殖密度(35、65、95、125 kg/m3)下革胡子鲶胃和肠道纹状缘厚度、柱状细胞高、壁厚、肌肉层厚以及肠道杯状细胞大小,分析不同养殖密度对革胡子鲶消化道的组织结构变化的影响。[结果]养殖30 d时,养殖密度仅影响胃和肠道中段的纹状缘厚度。35 kg/m3密度组的胃纹状缘厚度显著低于65、95和125kg/m3密度组。肠中段的纹状缘高在35、65和95 kg/m3密度组间无显著差异,但显著低于125 kg/m3密度组。养殖60 d时,35 kg/m3密度组的肠中段纹状缘显著厚于65、95和125 kg/m3密度组,但粘液细胞则显著小于65、95和125 kg/m3密度组,而65、95和125 kg/m3密度组间纹状缘高度和粘液细胞大小差异不显著。35 kg/m3密度组与65 kg/m3密度组、95 kg/m3密度组与125 kg/m3密度组肠中段和肠后段的肠壁厚和肌肉层厚无显著差异,但35和65 kg/m3密度组显著高于95和125 kg/m3密度组。[结论]养殖密度仅对革胡子鲶胃、肠道组织结构的少量测量指标有影响,与革胡子鲶的高耐受性和适宜高密度养殖的特点相一致。  相似文献   

Jaundice syndrome in African sharp‐tooth catfish,Clarias gariepinus (Burchell), associated with haemolytic Staphylococcus aureus     

O O Oladele  O L Ajayi  O O Olude  O O Stephen  A A Adediji  I O Arasi  U G Ntiwunka 《Journal of fish diseases》2012,35(12):945-947

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革胡子鲶骨骼系统研究     

陈成勋  孙金辉  马倩倩  粱超  孙敬锋 《安徽农业科学》2011,39(21):12916-12919,13097

[目的]研究革胡子鲶骨骼系统的组成及形态学特征。[方法]常规煮沸剥离方法得到完整骨骼标本和单个骨骼标本,对标本进行摄影与分析,并总结出相应的骨骼特征。[结果]革胡子鲶的骨骼系统具有单个犁骨,鳃盖边缘有棘,无眶蝶骨,腰带简单,有韧带与肩带相连。无骨鳞,无肌间骨,无续骨、顶骨,第3、4椎骨彼此固结。胸鳍具有1枚强大且坚硬的骨质棘,具有14对腹肋。共有骨骼426块,其中脑颅区域25块,咽颅区域71块,附肢骨骼234块,躯干部分96块。[结论]革胡子鲶骨骼系统具有一般硬骨鱼类的基本特征,同时此种鱼类的骨骼结构也具有鲶形目的特点。  相似文献   

大豆主要抗原蛋白对埃及胡子鲇肌肉营养成分的影响   总被引：2，自引：1，他引：1  

吴莉芳  邹瑞兴  王申  王洪鹤  孙泽威  秦贵信 《吉林农业大学学报》2009,31(6)

在室内单循环控温养殖系统中,给初始体质量为(15.14±0.05)g的健康埃及胡子鲇(Clarias lazera)投喂以鱼粉为动物蛋白源,鱼油、玉米油、糊精为能源,纤维素为填充物配制的3种等氮(粗蛋白质量分数为40%)、等能(15.8 MJ/kg)的半精制饲料(其中β-伴大豆球蛋白的添加量为40 mg/g,大豆球蛋白的添加量为60 mg/g),研究了大豆中主要抗原蛋白对埃及胡子鲇肌肉营养成分的影响。饲喂6周后的调查数据表明:在本试验条件下,β-伴大豆球蛋白和大豆球蛋白使埃及胡子鲇肌肉中粗蛋白含量极显著下降(P<0.01),对肌肉中粗脂肪含量、粗灰分含量影响不显著(P>0.05);使肌肉中氨基酸总量、必需氨基酸总量和鲜味氨基酸总量下降,但与对照组差异不显著(P>0.05)。因此,在埃及胡子鲇幼鱼的配合饲料中,β-伴大豆球蛋白的含量超过40 mg/g、大豆球蛋白的含量超过60 mg/g时,对其肌肉营养成分具有一定的影响。  相似文献   

Cloning and expression of two HSC70 genes in walking catfish Clarias macrocephalus (Günther, 1864) challenged with Aeromonas hydrophila     

Pornpun Poompoung  Dutrudi Panprommin  Prapansak Srisapoome  Supawadee Poompuang 《Aquaculture Research》2014,45(8):1319-1331

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Preliminary observations on effect of nano‐conjugated pheromones on Clarias batrachus (Linnaeus, 1758)     

Rupam Sharma  Mohd Ashraf Rather  Ramya Vijaykumar Leela  Himadri Saha  Suresh Babu Padinhate Purayil  Shabir Ahmad Dar  Sukham Munilkumar 《Aquaculture Research》2014,45(8):1415-1420

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