全文获取类型
收费全文 | 167篇 |
免费 | 45篇 |
国内免费 | 47篇 |
专业分类
农学 | 3篇 |
5篇 | |
综合类 | 31篇 |
农作物 | 6篇 |
水产渔业 | 213篇 |
畜牧兽医 | 1篇 |
出版年
2024年 | 4篇 |
2023年 | 5篇 |
2022年 | 2篇 |
2021年 | 10篇 |
2020年 | 10篇 |
2019年 | 13篇 |
2018年 | 11篇 |
2017年 | 15篇 |
2016年 | 22篇 |
2015年 | 7篇 |
2014年 | 10篇 |
2013年 | 14篇 |
2012年 | 13篇 |
2011年 | 14篇 |
2010年 | 4篇 |
2009年 | 8篇 |
2008年 | 8篇 |
2007年 | 10篇 |
2006年 | 13篇 |
2005年 | 9篇 |
2004年 | 8篇 |
2003年 | 10篇 |
2002年 | 7篇 |
2001年 | 4篇 |
2000年 | 8篇 |
1999年 | 8篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
排序方式: 共有259条查询结果,搜索用时 15 毫秒
11.
Emanuele Ponis René Robert Giuliana Parisi Mario Tredici 《Aquaculture International》2003,11(1-2):69-79
The Prymnesiophycea Pavlova lutheriwas produced using a traditional technique (batch mode in carboys) and by an innovative technique (semi-continuous mode in alveolarphotobioreactor). Two experimental feedingtrials were carried out with Pacific oyster(Crassostrea gigas) larvae with freshand preserved (4 °C and 1 °C)P. lutheri concentrates used inmonospecific and bispecific diets, incombination with fresh Chaetoceroscalcitrans forma pumilum. Nodifferences in terms of gross composition andlarval performance were noticed between thefresh algae biomass produced by the twotechniques. The low temperatures allowedpreservation of the nutritionalcharacteristics of the P. lutheri freshculture for the time period considered (27days). The P. lutheri concentratespreserved at 4 °C or 1 °C didnot differ substantially in terms ofnutritional value. Larvae fed a monospecificdiet of P. lutheri, both fresh andpreserved, exhibited a modest growth rate (<2.1 µm day–1), in some cases notsignificantly different from that observed inthe unfed larvae (the control), and a lowsurvival rate. In contrast, when Chaetoceros calcitrans forma pumilumwas used as a monospecific diet, good growthperformance (>4.5 µm day–1) and ahigh survival rate (>86%) were observed inlarvae. A substitution of 50% (trial 1) or80% (trial 2) of fresh C. calcitransforma pumilum with the preserved P. lutheri concentrates did not adverselyaffect growth rate or survival of C.gigas larvae. 相似文献
12.
太平洋牡蛎三倍体诱导及苗种培育技术研究 总被引:1,自引:0,他引:1
本文报道了采用细胞松弛素B(CB)诱导太平洋牡蛎三倍体的研究结果。研究结果表明,CB浓度为0.25mg/L,处理时间约20分钟,诱导三倍体的倍化率可达75%,至D型幼虫的孵化率为40-50%。1992-1996年其培育出三倍体贝苗1846.4万枚。 相似文献
13.
影响太平洋牡蛎人工苗种培育的主要因子 总被引:7,自引:0,他引:7
探讨了太平洋牡蛎苗种培育期间,亲贝产卵与授精,幼虫密度,饵料类别,固着基处理及投放等主要生态因子对幼虫孵化,生长发育与变态固着的影响,试验结果表明,把握好上述因子及参数,可提高幼虫的成活率及变态率,确保稳定的育苗效果。 相似文献
14.
Bivalves serve as an important aquaculture product, as they are the source of essential fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in our diet. However, their cultivation in the wild can be affected by fouling organisms that, in turn, affect their EPA and DHA content. The effects of fouling organisms on the EPA and DHA contents of cultivated bivalves have not been well documented. We examined the effects of fouling organisms on the EPA and DHA contents and condition index of cultured oysters, Crassostrea gigas, in an aquaculture system. We sampled two-year-old oysters from five sites in Shizugawa Bay, Japan, in August 2014. Most of the fouling organisms were sponges, macroalgae, and Mytilus galloprovincialis. A significant negative relationship existed between the DHA content in C. gigas and the presence of sponges and macroalgae. A lower C. gigas EPA content corresponded to a higher M. galloprovincialis fouling mass and a lower C. gigas condition index. This can be explained by dietary competition between C. gigas and M. galloprovincialis for diatoms, which were the main producer of EPA in our study sites. Our findings indicate that fouling organisms likely reduce the EPA and DHA content in cultivated oysters. Therefore, our results suggest that the current efforts to remove fouling organisms from oyster clusters is an effective strategy to enhance the content of EPA and DHA in oysters. 相似文献
15.
Floriane Boullot Caroline Fabioux Hlne Hgaret Pierre Boudry Philippe Soudant Evelyne Benoit 《Marine drugs》2021,19(7)
Pacific oysters (Crassostrea gigas) may bio-accumulate high levels of paralytic shellfish toxins (PST) during harmful algal blooms of the genus Alexandrium. These blooms regularly occur in coastal waters, affecting oyster health and marketability. The aim of our study was to analyse the PST-sensitivity of nerves of Pacific oysters in relation with toxin bio-accumulation. The results show that C. gigas nerves have micromolar range of saxitoxin (STX) sensitivity, thus providing intermediate STX sensitivity compared to other bivalve species. However, theses nerves were much less sensitive to tetrodotoxin. The STX-sensitivity of compound nerve action potential (CNAP) recorded from oysters experimentally fed with Alexandrium minutum (toxic-alga-exposed oysters), or Tisochrysis lutea, a non-toxic microalga (control oysters), revealed that oysters could be separated into STX-resistant and STX-sensitive categories, regardless of the diet. Moreover, the percentage of toxin-sensitive nerves was lower, and the STX concentration necessary to inhibit 50% of CNAP higher, in recently toxic-alga-exposed oysters than in control bivalves. However, no obvious correlation was observed between nerve sensitivity to STX and the STX content in oyster digestive glands. None of the nerves isolated from wild and farmed oysters was detected to be sensitive to tetrodotoxin. In conclusion, this study highlights the good potential of cerebrovisceral nerves of Pacific oysters for electrophysiological and pharmacological studies. In addition, this study shows, for the first time, that C. gigas nerves have micromolar range of STX sensitivity. The STX sensitivity decreases, at least temporary, upon recent oyster exposure to dinoflagellates producing PST under natural, but not experimental environment. 相似文献
16.
17.
6_二甲基氨基嘌呤诱导太平洋牡蛎三倍体_抑制受精卵第一极体释放 总被引:2,自引:0,他引:2
于 1996~ 1997年 ,用 6-二甲基氨基嘌呤 ( 6-DMAP)抑制受精卵第一极体的释放 ,诱导太平洋牡蛎产生三倍体。选用L16( 45)设计 ,进行三因素四水平的正交试验 :6-DMAP浓度 ,设15 0、30 0、45 0和 60 0μmol/L ;诱导时机 (即精卵混合后的时间 ) ,设 10、15、2 0和 2 5min ;诱导持续时间 ,设 10、15、2 0和 2 5min。试验平行重复二次。结果为最高三倍体诱导率为 ( 71.3± 1.2 ) % ,该实验组胚胎孵化率为 ( 5 5 .5± 3.1) % ,D形幼虫畸形率为 ( 10 .7± 1.6) %。根据直观分析结果 ,得出诱导太平洋牡蛎三倍体各因素的最优水平组合 :水温 2 5~ 2 5 .5℃ ,精卵混合 10min时 ,将受精卵浸泡在含 6-DMAP 60 0μmol/L的海水中 15min ;决定三倍体产生的三因素的主次顺序 :6-DMAP浓度→诱导时机→诱导持续时间。 6-DMAP浓度对三倍体诱导率影响显著 ,但诱导时机和诱导持续时间不显著。 相似文献
18.
巨蛎属牡蛎遗传多样性研究 总被引:31,自引:3,他引:28
用RPD方法获得的几种牡蛎间的遗传距离表明,我国北方海域确定存在大连湾牡蛎,褶牡蛎和近江牡蛎三种自然种。,它们同属巨蛎属。大连湾牡蛎,褶牡蛎和太平洋牡蛎互为姊妹种,近江牡蛎与它们互为非姊妹种。有无长牡蛎以及长牡蛎与上述三种牡蛎的关系有待进一步研究 相似文献
19.
20.