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131.
为了研究复合生物保鲜剂对半滑舌鳎的保鲜效果,以复合生物保鲜剂结合4℃冷藏处理试样,通过正交试验确定复合保鲜剂的最佳配比为:0.4%ε-聚赖氨酸、1.5%壳聚糖和0.05%乳酸链球菌素,并通过感官检验、菌落总数、TVB-N含量和pH值的测定对其进行验证试验。结果表明:复合组贮藏至第10天,感官评分为10.5分,菌落总数lg(cfu/g)=5.63,TVB-N含量达到35.1mg/100g,pH值为7.36,各项指标均显著优于对照组(P0.05),将4℃贮藏期有效延长了5~6d。 相似文献
132.
This study investigated the effect of n‐3 to n‐6 fatty acid ratios in broodstock diets on reproduction performance, fatty acid composition of eggs and gonads of tongue sole Cynoglossus semilaevis. Broodstock were fed five isonitrogenous and isoenergetic diets for 60 days. The supplemented lipids were prepared by a combination of fish oil and soybean oil inclusion FO (fish oil); FSO1 (fish oil: soybean oil = 7:1); FSO2 (fish oil: soybean oil = 2.2:1); FSO3 (fish oil: soybean oil = 1:1); FSO4 (fish oil: soybean oil = 1:4.3) as lipid sources with different n‐3 to n‐6 fatty acid ratios 10.40, 5.21, 2.81, 1.71 and 0.87. Results showed that relative fecundity, fertilization rate and survival rate of larvae at 7 days posthatching were all higher in broodstock fed FSO1 and FSO2 diet and significantly (P < 0.05) decreased in groups fed FSO3 and FSO4 diets. The best result in starvation tolerance test was obtained in FSO2 diet. The present study suggests that n‐3 and n‐6 PUFA ratio in broodstock diet has a considerable effect on spawning performance, egg and larval quality for C. semilaevis. 相似文献
133.
Jinghui Fang Xiangli Tian Shuanglin Dong Jianguang Fang Jihong Zhang 《Aquaculture Research》2014,45(9):1523-1532
The compensatory growth, body composition and energy budget of juvenile tongue sole, Cynoglossus semilaevis (Günther, 1873), subjected to an unfavourable temperature were investigated during 56 days experiment. Fish were divided into four groups including three groups, which were reared at 16°C for 1, 2 and 3 weeks, respectively, then returned to 22°C (recorded as A1, A2 and A3) and a control group (continuously reared at 22°C, C). At the end of the experiment, the body mass was significantly higher in A1 than that in the control (P < 0.05), which indicated over compensatory growth occurred in A1 fish. No significant difference was found in body mass among A2, A3 and C fish (P > 0.05), which indicated complete compensatory growth occurred in A2 and A3 fish. The underlying mechanisms for compensatory growth could be attributed to an improved energetic efficiency resulting from reduced metabolic expenditure and higher feed efficiency during the period of recovery. Furthermore, the fish exposed to lower temperature for 3 weeks showed hyperphagia. The results suggested that the juvenile C. semilaevis exposed to lower temperature for a suitable period (1–3 weeks) used feed more efficiently. This approach may be a useful rearing strategy for indoor culture of C. semilaevis. 相似文献
134.
通过解剖和HE染色观察患红皮病半滑舌鳎各器官的病理组织学变化.病鱼体表充血、出血,无眼侧皮肤溃烂、淤血,鳃丝溃烂、有大量粘液;肝胰脏和脾脏肿大、淤血出血;小肠充血、出血.皮肤变性坏死以及粘膜下层出血,肌纤维间有血红细胞的渗透和淤积,腮小腺上皮变性坏死,出鳃动脉充血.心肌纤维结构松散、出现大面积断裂,肝脏细胞水肿、细胞变性、细胞核集中.肾脏间质出现局灶性的大面积组织崩解和坏死小肠粘膜下层坏死脱落、出血.结果显示:患红皮病的半滑舌鳎多个组织器官表现为充血、出血和坏死. 相似文献
135.
以初始体重为(3.70±0.01)g的半滑舌鳎(Cynolossus semilaevis Günter)幼鱼为实验对象,研究小麦胚芽油替代鱼油对其生长、体成分、血清生化指标及脂肪代谢酶的影响。以添加7.5%的鱼油组为对照组,用小麦胚芽油分别替代0、20%、40%、60%、80%、100%的鱼油配制成6组等氮等能饲料,在海水循环养殖系统中进行68 d的养殖实验。结果显示:饲料中小麦胚芽油替代鱼油对半滑舌鳎幼鱼存活率无显著影响;随着替代水平的升高,特定生长率和蛋白质效率降低,饲料系数升高。各组间脏体比、肝体比和肥满度均无显著性差异。肝脂肪含量随替代水平的升高而升高,而全鱼脂肪含量降低;80%和100%替代组全鱼水分含量显著高于对照组(P0.05)。随着饲料中小麦胚芽油水平的升高,鱼体组织中亚油酸和亚麻酸含量显著升高(P0.05),EPA和DHA含量显著降低(P0.05)。各组间血清谷丙转氨酶、谷草转氨酶、碱性磷酸酶和溶菌酶活性均无显著性差异(P0.05);80%和100%替代组甘油三酯和总胆固醇含量显著高于对照组(P0.05);血清丙二醛含量呈降低趋势。随着替代水平的升高,肝脂蛋白酯酶、肝酯酶、总酯酶、脂肪酸合成酶以及肠道脂肪酶活性升高。研究表明,在本实验条件下,小麦胚芽油可替代60%的饲料鱼油而不影响半滑舌鳎幼鱼的生长;小麦胚芽油的添加,促进了鱼体脂肪代谢,抑制脂质过氧化作用,但显著改变了鱼体脂肪酸组成。 相似文献
136.
17β-雌二醇对半滑舌鳎性分化和生长的影响 总被引：3，自引：0，他引：3
试验将25日龄半滑舌鳎稚鱼放入不同浓度(1、3、10和30μg·L-1)的17β-雌二醇水溶液中,每天浸泡2h,连续处理65d,研究17β-雌二醇对半滑舌鳎(Cynoglossus semilaevis Gunther)性分化、性比、生长和死亡率的影响。结果表明,17β-雌二醇使半滑舌鳎卵巢分化提前约5d,使精巢分化推迟20d;4种浓度17β-雌二醇处理组(1、3、10和30μg·L-1)的雌性率分别为74%、82%、88%和97%,与对照组相比差异显著(P<0.05)。同时,17β-雌二醇处理对半滑舌鳎的早期生长和死亡率无显著影响。 相似文献
137.
138.
紫外灭活的异源和同源精子诱导的半滑舌鳎单倍体胚胎发育过程比较 总被引：2，自引：0，他引：2
为探讨在雌核发育研究中紫外灭活的异源精子的细胞质等是否对“受精”卵的发育产生影响,本文对紫外灭活的异源和同源精子诱导的半滑舌鳎单倍体胚胎发育过程进行了比较研究。结果显示,在胚盘形成期、二细胞期、多细胞期和囊胚期,异源和同源精子诱导的半滑舌鳎单倍体胚胎发育时序及形态无显著差异。之后,灭活的异源精子诱导的单倍体胚胎发育速度比同源精子诱导的单倍体胚胎要慢,至受精后18h 48min已慢1个发育时期,说明在发育前期,异源精子诱导的单倍体胚胎发育阻力更大。肌节期后,同源精子诱导的半滑舌鳎单倍体胚胎发育也严重受阻,与异源精子诱导的单倍体胚胎发育速度无显著差异;且单倍体胚胎陆续大量死亡,但异源精子诱导的单倍体胚胎死亡率明显要大于同源精子诱导的单倍体胚胎。异源和同源精子诱导的单倍体胚胎孵出的仔鱼均表现有单倍体综合症,外形基本一致。为确保紫外线处理将精子完全灭活,当紫外灭活的异源和同源精子诱导的半滑舌鳎单倍体胚胎发育至原肠后期时,各取30粒用倍性分析仪检测其倍性,结果显示全为单倍体。 相似文献
139.
在（22±1）℃海水温度下,以初始体重（180～220）g的半滑舌鳎为研究对象,分别在配合饲料中以土霉素浓度为0mg·kg^-1和125mg·kg^-1拌料投喂,研究半滑舌鳎后肠中好氧菌耐药率的变化情况,同时使用高效液相色谱检测半滑舌鳎肌肉中土霉素的残留量。结果表明：在添加16mg·kg^-1土霉素的选择性培养基上,半滑舌鳎后肠好氧菌其耐药率从开始投喂时的（12．32±2．36）％增加到停药期间最大值（43．15±4．20）％;而在160mg·L^-1浓度土霉素的培养基上,其耐药率从开始的（5．6±2．94）％增加到（18．64±0．45）％。土霉素残留实验表明,在投喂药期间,土霉素在肌肉中的残留量处于（2．23±0．40）～（3．14±0．15）mg·kg^-1之间,但投药期结束后,残留量迅速下降,直到第6天降到检测限以下。本研究结果表明,在土霉素作用下,实验组耐药率出现较大的变化,但在停药16天时基本恢复到正常水平。 相似文献
140.
工厂化养殖半滑舌鳎生长、摄食和水质的变化特征及规律 总被引：6，自引：0，他引：6
在封闭循环水养殖条件下,进行6×2双因素随机设计动物试验,即6个体重阶段(45～550 g)和2个密度水平(高密度比正常密度提高20%～30%),共形成12个处理,每处理3个重复,试验期44 d.探寻我国北方工厂化养殖半滑舌鳎生长、摄食和水质的变化特征及规律.结果表明:(1)日增重、日均摄食量、饲料系数等绝对指标与体重阶段呈正相关;氨氮、COD等水质指标相对浓度与体重阶段呈负相关;(2)常规水质条件下,高密度养殖不利于生长、摄食和降低水体有害物,其副作用起初并不明显,随着试验时间的推移不断加大;(3)新发现半滑舌鳎的日摄食率为0.43%～0.92%,对常规鱼类投饲率推荐范围(2%～5%)提出了质疑,为现代海水养殖精准饲喂和清洁生产提供了科学依据;(4)本试验条件下,体重45～550 g阶段半滑舌鳎的特定生长率为(1.42%～0.50%)/d,饲料系数为0.68～0.86,水中氨氮为8.45～1.51 mg/(kg.h). 相似文献