Cd（Ⅱ）胁迫对盐藻生长、抗氧化系统及线粒体膜电位的影响     

向蓓  赵文  王媛  田丹 《大连水产学院学报》2012,27(1):64-68

研究了不同浓度的Cd（Ⅱ）（0、0.01、0.10、1.00 mmol/L）对盐藻Dunaliella salina生长、抗氧化系统及线粒体膜电位的影响。结果表明：在0.01 mmol/L和0.10 mmol/L的Cd（Ⅱ）胁迫下,盐藻的生长几乎不受影响,而1.00 mmol/L的Cd（Ⅱ）对盐藻生长有显著的抑制作用（P〈0.05）;盐藻中丙二醛（MDA）的含量随着Cd（Ⅱ）处理浓度的变化先升高后降低;在1.00 mmol/L的Cd（Ⅱ）胁迫和诱导下,盐藻超氧化物歧化酶（SOD）的活性最大;总抗氧化力（T-AOC）仅在0.10 mmol/L的Cd（Ⅱ）胁迫下明显升高;各试验组盐藻线粒体膜电位受Cd（Ⅱ）胁迫的影响不明显。  相似文献   

巴夫杜氏藻GAPDH基因部分序列的克隆     

尚常花  朱顺妮  袁振宏  王忠铭 《广东农业科学》2011,38(13):126-127,134

3-磷酸甘油醛脱氢酶(GAPDH)是糖酵解途径中的一个关键酶,由于其基因表达量相对恒定.因此在定量和半定量PCR试验中常被用作内源参照基因来确定目标基因的相对表达量.从巴夫杜氏藻(Dunaliella pmva)中克隆GA PDH基因,并对基因序列进行了分析.以巴夫杜氏藻cDNA为模板,根据GAPDH的保守序列设计引物...  相似文献   

盐藻DNA对大豆幼苗耐盐性的影响     

郭金耀  杨晓玲 《安徽农业科学》2011,39(25):15241-15242

[目的]增强大豆幼苗的耐盐性。[方法]用0.1 mol/L氯化钙溶液浸泡大豆种子4 h后,将其浸入5 mg/L盐藻DNA水溶液中,蒸馏水浸泡作为对照组。选取长势一致的大豆幼苗,用蒸馏水冲洗干净幼苗的根部,栽于含3 g/L氯化钠的MS培养液中,处理与对照各60株。检测大豆幼苗耐盐适应性的变化。[结果]处理组的植株鲜重和株高分别较对照高出33.6和22.1个百分点,存活率高出46.7个百分点,根数减少了26.2个百分点,根长缩短了17.6个百分点。[结论]盐藻DNA提高了大豆幼苗的耐盐性。  相似文献   

不同提取方法对盐藻β-胡萝卜素产量的影响     

滕长英  张立  邹宁  吴云杰 《安徽农业科学》2008,36(29)

[目的]研究不同提取方法对盐藻β-胡萝卜素产量的影响,以改进盐藻β-胡萝卜素的提取工艺。[方法]首先比较了丙酮∶甲醇(7∶2)和石油醚2种常用提取试剂对盐藻β-胡萝卜素提取效率的影响;然后以丙酮-甲醇(7∶2)为提取试剂,分别从提取时间、提取温度、固液比3个方面对其提取工艺进行了优化。[结果]相对于石油醚,丙酮∶甲醇(7∶2)对盐藻β-胡萝卜素的提取效率较高。4℃和25℃浸提温度下β-胡萝卜素的得率没有明显差异。分3次提取时,5 m in的浸提时间即可获得较高的提取效率。在1~7 m l的提取剂使用范围内,随着提取剂用量的增加β-胡萝卜素的提取效率有所增加,但增加幅度不是很大。[结论]以丙酮∶甲醇(7∶2)为提取试剂,在25℃下浸提5 m in 3次可以得到较高的提取效率。  相似文献   

The Potential Use of Microalgal Carotenoids as Dietary Supplements and Natural Preservative Ingredients     

Hanaa H. Abd El-Baky  Gamal S. El-Baroty 《Journal Of Aquatic Food Product Technology》2013,22(4):392-406

Dunaliella salina has been selected as the most valuable microalgae for production of carotenoids as a source of natural pigment when grown under defined conditions. Compared with optimal conditions, D. salina contained higher amounts of total carotenoids (TCAR, 12.6%, dw)—including β-carotene (60.4%, of total carotenoids), astaxanthin (17.7%), zeaxanthin (13.4%), lutein (4.6%), and cryptoxanthin (3.9%)—when grown under stress conditions. These TCAR showed greater in vitro antioxidant potential by inhibition of lipid peroxidation of rat liver microsomes induced by Fe⁺⁺/ascorbate and Fe⁺⁺/H₂O₂ model systems than butylated hydroxytoluene (BHT). The toxicological evaluation of D. salina functional ingredients as an antioxidant and natural food colorant was conducted, in which male albino rats were fed ad libitum for 6 weeks with one of the following diets: standard diet (S), S diets containing 0.5 g trans β-carotene/kg diets (S + 0.5 β C), S + 0.5 g D. salina carotenoids/kg diets (S + 0.5 DC), and S + 1.0 g D. salina carotenoids/kg diets (S + 1.0 DC). Generally, no mortality or treatment-related toxicological signs were observed in any of the rats during the 6 weeks of the experiment. The concentrations of glucose and glycogen in liver and glucose, total proteins, bilirubin, and creatinine as well as enzymes activities of aspartate (AST) and alanine aminotransferases (ALT), and alkaline phosphatase (ALP) in plasma were not significantly different from each other in all four diets. In contrast, the levels of total lipids and lipids components showed significant differences in all groups. Groups S + 0.5 βC, S + 0.5 DC, and S + 1.0 DC showed a significant decrease in concentrations of total lipids, triglycerides, total cholesterol, and phospholipids in either plasma or hepatic tissues compared with S group. The results showed that D. salina carotenoids could be potentially rich sources of natural antioxidants and food colorants and have hypolipidemic and hypocholesterolemic properties.  相似文献   

Fish antibiotherapy: bioencapsulation of flumequine using adult brine shrimp (Artemia salina)     

João Gomes  Cristina Lobo Vilela  Ricardo Bexiga  Gonçalo D Nunes  Nuno Pereira  & Lina M Cavaco 《Aquaculture Research》2007,38(6):613-617

Optimization of antibiotic delivery strategies to aquatic environment and to the specific characteristics of the target species is essential for the improvement of bacterial infection control measures. This work aimed at standardizing the use of Artemia salina to deliver flumequine to fish as antimicrobial treatment. Adult Artemia were used to bioencapsulate flumequine. A flumequine concentration of 358 μg mL⁻¹ was found adequate to perform bioencapsulation during 24 h without causing mortality. Antibiotic concentration in Artemia, quantified by means of a microbiological assay based on MIC determination, using Escherichia coli ATCC 25922 as control strain was 256.55 mg g⁻¹ (±71.22). The therapeutic doses of 10 mg kg⁻¹ BW, calculated on the basis of a consumption of about 4% BW/day, would then be delivered by the consumption of 7.8 Artemia g⁻¹ of fish.  相似文献   

C02浓度对光生物反应器高效培养的湛江叉鞭金藻和盐藻的影响     

吴垠 《水产学报》2004,28(6):741-744

The effects of CO2 concentration on the growth and photosynthesis of Dicrateria zhang, jiangensis and Dunaliella sp, in 100 liter air lift photobioreactors were investigated. The results showed that biomass of both algae significantly increased in high CO2 concentration (above 700μL ? L^-1) compared to low CO2 concentration (350μL?L^-1) ,and the culture periods were shorter, However, no significant difference as observed in growth rate when the alga cultured in CO2 concentration of 700μL? L^-1 and 1000μL? L^-1, respectively. These for the maximum photosynthetic rate(Pm) and saturating irradiance level(Ik) of the two species remarkably raised with the elevated CO2 concentration, so was the initial slope(α)of Dunaliella sp.  相似文献   

电动多旋翼植保无人机低容量喷雾防治玉米三点斑叶蝉的应用研究   总被引：1，自引：0，他引：1  

赵冰梅  丁丽丽  张强  董红强 《植物保护》2018,44(1):186-189

利用KT-10-Ⅱ型四旋翼植保无人机开展玉米灌浆期三点斑叶蝉的防治试验,研究无人机飞行高度、飞行速度、施药液量以及喷雾助剂等因素对农药雾滴在玉米冠层的沉积分布影响及对三点斑叶蝉防效的影响。结果表明:在无人机飞行高度为距玉米株冠顶部1m、飞行速度6m/s、施药液量15L/hm2,并加入1.5%喷雾助剂"迈飞"的条件下,喷雾雾滴在玉米冠层上部、中部和下部的沉积密度分别达到41.9、27.3和14.9个/cm2,对三点斑叶蝉药后1d防效可达91.3%,药后14d防效96.8%以上,速效性和持效性均较理想。  相似文献   

绿色杜氏藻DvGGPS生物信息学及转录差异研究     

周静  龚一富  马颖瑞  刘浩  朱帅旗  王何瑜  严小军 《核农学报》2017,31(4)

香叶酰香叶酰焦磷酸合成酶(GGPS)能够催化焦磷酸异戊烯酯(IPP)与二甲基丙烯焦磷酸(DMAPP)反应形成二萜化合物通用前体香叶酰基香叶酰焦磷酸(GGPP)。为深入了解GGPS蛋白在类胡萝卜素合成途径中的作用,通过Illumina Hi Seq TM 2000高通量测序获得Dv GGPS基因c DNA全长序列,对GGPS基因序列进行生物信息学分析,并研究花生四烯酸(AA)、乙酰水杨酸(ASA)和硫酸铈铵(ACS)对Dv GGPS基因转录水平的影响及绿色杜氏藻类胡萝卜素含量变化。生物信息学分析结果表明,Dv GGPS基因c DNA全长2 229 bp,含1 041 bp开放阅读框,编码346个氨基酸。GGPS蛋白质序列理论等电点(p I)为5.82,相对分子质量为37.66 k Da,该蛋白为疏水性蛋白,无信号肽和跨膜区域。二级结构预测显示该蛋白分子中α-螺旋结构最多,占57.23%。同源比对结果表明,绿色杜氏藻GGPS蛋白与雨生红球藻亲缘关系最近。qRT-PCR结果表明,62.5 mg·L~(-1)AA、50 mg·L~(-1)ASA和0.8 mg·L~(-1)ACS处理的Dv GGPS基因转录水平达到最高,此时类胡萝卜素含量也均有提高,说明绿色杜氏藻类胡萝卜素的生物学合成可能是通过诱导Dv GGPS基因表达实现的,Dv GGPS基因在类胡萝卜素生物合成中起关键作用。Dv GGPS基因的克隆及表达调控研究为今后探明类胡萝卜素积累的分子机理提供了重要参考,也为进一步通过代谢工程手段提高绿色杜氏藻类胡萝卜素含量奠定了理论基础。  相似文献   

C、N、P比例对2株盐藻生长和生化组成的影响   总被引：1，自引：0，他引：1  

王培磊 《水产科学》2010,29(3)

采用正交设计法研究了C、N、P比例对2株盐藻OUN04和OUN05生长、β-胡萝卜素积累、叶绿素a合成、脂肪酸组成、蛋白质含量的影响。试验结果表明,适当调节C、N、P比例可以促进盐藻OUN04生长、β-胡萝卜素积累、叶绿素a合成,盐藻OUN04最大细胞密度114.2×104个/ml、β-胡萝卜素最大含量100.4 mg/g、叶绿素a最高含量144.6 mg/g分别出现在C、N、P比例为12∶1∶0.05、12∶2∶0.1、12∶1∶0.05的处理组中,且均与对照组差异显著;适当调节C、N、P比例也可以提高盐藻OUN04脂肪酸和蛋白质含量。盐藻OUN05细胞最大密度101.2×104个/ml、β-胡萝卜素最高含量109.2 mg/g、叶绿素a最高含量108.7 mg/g分别出现在C、N、P比例为6∶2∶0.05、6∶0.5∶0.1、12∶2∶0.1的处理组中,且均与对照组差异显著;最大蛋白含量34.41%出现在6∶1∶0.05的处理组中,但与对照组(33.17%)差异不显著。  相似文献