Comparative Studies of β-carotene and Protein Production From Dunaliella salina Isolated From Lake Hoze-soltan,Iran     

Saideh Tavallaie  Marjan Emtyazjoo  Khosrow Rostami  Haleh Kosari 《Journal Of Aquatic Food Product Technology》2013,22(1):79-90

A novel Dunaliella salina (D. salina) was isolated from Hoze-soltan Lake, Iran. The investigated strain was capable of producing carotenoids and protein, using different NaCl concentrations and pH variations in Hoze-soltan saline water. The experiment was carried out within 42 days. The results indicated that protein production and pigmentation correlated well with growth rate. The optimum amount for growth, carotenogenesis, and protein production was determined in a culture with 10% NaCl and pH 8.5. After 42-days incubation, 14.95 ± 0.6 μg/mL of carotenoids and 186 ± 8.6 μg/mL of protein were obtained in this medium. Further observation revealed that growth was inhibited in all samples with 30% NaCl. The amount of each biosynthesized component by Dunaliella salina was in accordance with growth stage, cell number, and growth pattern of microalgae.  相似文献   

盐藻醛缩酶的盐适应性及其与物质积累的关系     

郭金耀  杨晓玲 《水产科学》2013,(8)

在不同N aC l质量浓度下培养盐藻,分析盐藻醛缩酶的盐适应性及其与物质积累的关系。试验结果表明,在NaCl质量浓度为40～120 g/L时,盐藻醛缩酶活性与盐度具有正相关性;在NaCl质量浓度为120～200 g/L时,盐藻醛缩酶活性与盐度具有负相关性;在NaCl质量浓度为120 g/L时,盐藻醛缩酶活性最高。盐藻醛缩酶是一种高盐适应酶。盐藻醛缩酶活性与盐藻细胞密度、叶绿素形成和蛋白质积累均呈正相关关系。在盐藻醛缩酶活性最高时,盐藻物质积累量最多。  相似文献   

杜氏盐藻耐盐分子机制的研究进展     

武天祥  柴晓杰  丛玉婷  刘丽颖  刘艺琼 《农业科学与技术》2016,(2)

盐藻是已知最为耐盐的真核生物之一,是研究植物高盐适应的最理想的模式生物.近年来,随着分子生物学技术的发展,盐藻耐盐分子机制的研究也备受国内外学者的瞩目.本文就盐藻适应高盐环境的渗透调节、盐藻耐盐相关基因和蛋白质以及盐胁迫信号转导途径等方面的研究做一简要的综述.  相似文献   

玉米三点斑叶蝉在玉米田的空间分布型     

屈荷丽  杜娟  雷勇辉  赵冰梅  张建萍  李亦松  王少山 《植物保护》2016,42(6):149-153

本文应用聚集度指标检验法,以及Iwao的回归模型分析法、Taylor幂法则,对玉米三点斑叶蝉成虫、若虫的空间分布型进行了测定分析,结果表明:成虫、若虫空间分布型均为聚集分布,基本成分为个体群,个体间相互吸引,聚集程度随着密度增大而增高。成虫的聚集是由环境条件所引起;若虫单株密度低于5.08头时聚集是由环境条件引起,单株密度高于5.08头聚集是由昆虫行为和环境共同作用引起的。建立了玉米三点斑叶蝉成虫、若虫田间调查理论抽样数模型。调查期间,玉米三点斑叶蝉成、若虫在玉米植株下部数量多于中部,中部数量又多于上部。  相似文献   

外源一氧化氮缓解盐胁迫下杜氏盐藻氧化损伤的初步研究   总被引：1，自引：0，他引：1  

卢雪景  刘红涛  柴玉荣  朱大恒  罗清菊  吕鹏举  薛乐勋 《河南农业科学》2009,(5)

研究外源一氧化氮(NO)供体硝普钠(sodium nitroprusside,SNP)缓解3.0 mol/L NaCl胁迫对杜氏盐藻的氧化损伤作用并探讨其初步机理。结果表明:与单独盐胁迫相比,添加低浓度的SNP(0.5μmol/L和1μmol/L)能促进杜氏盐藻的生长,显著提高其叶绿素含量,并增强了超氧化物歧化酶(SOD)的活性(P<0.05)。其中,1μmol/L SNP处理24 h后SOD活性提高了1.6倍。此外,半定量RT-PCR结果显示,低浓度的SNP(0.5μmol/L和1μmol/L)对盐胁迫下杜氏盐藻Fe-SOD基因的转录具有促进作用,24 h时作用显著(P<0.05),表达量分别提高了21.18%和27.41%,而高浓度的SNP(50μmol/L)抑制Fe-SOD基因的表达,加剧了盐胁迫带来的氧化伤害。  相似文献   

盐胁迫条件下盐藻的生长及特异表达蛋白的研究   总被引：1，自引：0，他引：1  

王晓庆  柴晓杰  王向东  靳非  王宇航 《水产科学》2009,28(2)

采用不同NaC1浓度培养盐藻.通过分光光度法记录其生长状态.试验结果表明,在NaC1浓度为1.0～2.0 mol/L时.盐藻生长状态良好;NaCl浓度为3.0～4.0 mol/L时盐藻生长缓慢;盐藻经不同NaCI浓度培养0.5、5、23 h后抽提蛋白,所获得的粗提蛋白经SDS-聚丙烯酰胺凝胶电泳分离,观察不同NaCI浓度培养液中生长的盐藻可溶性蛋白质SDS-PAGE图谱.发现在低NaC1浓度(1.0～2.0 mol/L)下培养时23、51 kD蛋白略有变化但变化不大;在高NaC1浓度(3.0～4.0 mol/L)下胁迫5、23 h时23 kD蛋白显著增加;高NaC1胁迫23 h时51 KD蛋白含量下降明显.验结果证明51、23 kD蛋白可能与盐藻耐盐机制有关.  相似文献   

巴夫杜氏藻1,5-二磷酸核酮糖羧化酶/加氧酶小亚基基因的克隆和分析   总被引：2，自引：0，他引：2  

尚常花  朱顺妮  袁振宏  吕鹏梅  王忠铭 《水产学报》2011,35(5):668-674

1,5-二磷酸核酮糖羧化酶/加氧酶(Rubisco)是光合作用中的一个关键酶,其小亚基rbcS具有调控羧化反应催化效率和影响该酶对二氧化碳/氧气底物特异性的功能。从巴夫杜氏藻中克隆rbcS基因及其5′-上游序列,并对基因及5′-上游序列进行了分析。根据已知rbcS基因保守核苷酸序列设计引物,分别克隆到1 841 bp的DNA和380 bp的cDNA序列。以此为基础,使用染色体步移(Genome walking)和cDNA 末端快速扩增(RACE)技术,获得了799 bp的5′端DNA序列和500 bp的3′端cDNA序列。序列分析发现,巴夫杜氏藻rbcS DNA全长为2 031 bp(不包括476 bp 5′-上游序列),cDNA全长包括570 bp开放读码框(GenBank登录号:HQ315783)和294 bp 3′端非翻译区。5′-上游序列区域存在一系列预测的顺式作用元件。该研究旨在为后继的rbcS 基因的功能和表达研究、Rubisco的遗传改造奠定基础。同时,rbcS启动子因其可驱动基因高效表达而引起广泛关注,因此获得的rbcS 5′-上游序列经验证和优化后,可用于在嗜盐微藻中驱动转基因的高效表达以及完善微藻转化系统。  相似文献   

盐藻运动特性及其影响因素     

郭金耀  杨晓玲 《水利渔业》2011,32(5)

为了揭示盐藻的运动特性,在不同条件下观察了玻璃U形管两端盐藻的密度变化。结果表明,盐藻运动受光照、ATP、丙二酸钠和粘度的影响。盐藻具有向光源方向聚集的运动特性;供给ATP时盐藻运动加速,加入丙二酸钠时盐藻运动减慢,盐藻的运动是一个消耗能量的过程;增加介质粘稠度会使其运动速度减慢。  相似文献   

Mitosis Inhibitors Induce Massive Accumulation of Phytoene in the Microalga Dunaliella salina     

Yanan Xu  Patricia J. Harvey 《Marine drugs》2021,19(11)

Phytoene is a colourless natural carotenoid that absorbs UV light and provides antioxidant and anti-inflammatory activities as well as protection against photodamage. It is therefore valued for its skin health and aesthetic benefits by the cosmetic industry, as well as by the health food sector. The halotolerant green microalga Dunaliella salina is one of the richest sources of natural carotenoids. We have previously investigated the over-production of phytoene in D. salina after cultivation with the well-characterised mitosis inhibitor, chlorpropham. In this study, 15 herbicides with different modes of action were tested for their potential to promote phytoene accumulation. All herbicides showed different levels of capabilities to support phytoene over-production in D. salina. Most significantly, the two mitosis inhibitors tested in this study, propyzamide and chlorpropham, showed similar capacities to support the over-production of phytoene by D. salina cultures as phytoene desaturase inhibitors. The cellular content of phytoene increased by over 10-fold within 48 h of treatment with the mitosis inhibitors compared to untreated cultures. Results indicate a general effect of mitosis inhibitors on phytoene accumulation in D. salina. Furthermore, red light was found to significantly enhance the phytoene yield when used in combination with effective inhibitor treatments. Red light can be applied to maximize the production of phytoene from D. salina.  相似文献   

Potential Cytotoxic Effects of Peptide Fractions from Dunaliella salina Protein Hydrolyzed by Gastric Proteases     

Maliheh Darvish  Seyed-Omid Ranaei-Siadat  Mahsa Sedighi 《Journal Of Aquatic Food Product Technology》2018,27(2):165-175

Dunaliella salina is a halotolerant microalga with high protein content. Until now, there was no report on biological properties of peptides prepared by enzymatic digestion of D. salina proteins. Therefore, antimicrobial and antiproliferative effects of the abundant protein of D. salina were investigated. The extracted proteins were separated by fast protein liquid chromatography (FPLC), and protein hydrolysis was carried out with intestinal proteases such as trypsin and chymotrypsin. The antimicrobial and antiproliferative activities of the hydrolyzed peptides were examined on Escherichia coli, Staphylococcus aureus, and Helicobacter pylori, and colon cancer cell lines, respectively. The results demonstrated that 63 kDa protein and its derived peptides caused a decrease in bacteria growth, and <3 kDa peptide fraction significantly reduced SW480 cell viability. Therefore, the peptide fractions with antimicrobial and antiproliferative activities are worthy of further investigation as functional food ingredients for health benefits.  相似文献