Cd（Ⅱ）胁迫对盐藻生长、抗氧化系统及线粒体膜电位的影响     

向蓓  赵文  王媛  田丹 《大连水产学院学报》2012,27(1):64-68

研究了不同浓度的Cd（Ⅱ）（0、0.01、0.10、1.00 mmol/L）对盐藻Dunaliella salina生长、抗氧化系统及线粒体膜电位的影响。结果表明：在0.01 mmol/L和0.10 mmol/L的Cd（Ⅱ）胁迫下,盐藻的生长几乎不受影响,而1.00 mmol/L的Cd（Ⅱ）对盐藻生长有显著的抑制作用（P〈0.05）;盐藻中丙二醛（MDA）的含量随着Cd（Ⅱ）处理浓度的变化先升高后降低;在1.00 mmol/L的Cd（Ⅱ）胁迫和诱导下,盐藻超氧化物歧化酶（SOD）的活性最大;总抗氧化力（T-AOC）仅在0.10 mmol/L的Cd（Ⅱ）胁迫下明显升高;各试验组盐藻线粒体膜电位受Cd（Ⅱ）胁迫的影响不明显。  相似文献   

不同浓度氮、磷对杜氏盐藻生长的影响     

王俊  马玉心  崔大练  范彩彩 《浙江水产学院学报》2012,(2):142-146

在实验室条件下,研究了不同氮（0、0.1、0.4、0.8、1.0、1.2 g/L）、磷（0、0.015、0.025、0.030、0.045、0.060 g/L）浓度下杜氏盐藻生长增殖的关系和特征,以及氮磷比（c（N）/c（P））变化等对该藻生长的影响。实验结果表明：杜氏盐藻对氮、磷有着较强的适应能力,浓度增大藻增长率增大,浓度继续增大反而抑制藻的增长,属于营养依赖型藻类。不同氮质量浓度梯度对杜氏盐藻生长具有显著性影响（P〈0.05）,但对杜氏盐藻生长率K的影响不显著（P〉0.05）,最适宜的氮浓度为1.0 g/L;不同磷质量浓度梯度对杜氏盐藻的生长和生长率K具有显著性的影响（P〈0.05）,最适宜的磷浓度为0.025 g/L。当c（N）/c（P）为40时,最适合杜氏盐藻的生长。  相似文献   

Iron‐Binding Properties of TTf,a Salt‐Induced Transferrin from the Alga Dunaliella salina     

《Journal of plant nutrition》2013,36(10-11):2081-2091

Abstract

Iron deficiency induces accumulation of a transferrin‐like protein, TTf, in plasma membranes of the halotolerant alga Dunaliella salina. In vivo iron binding and uptake studies provided evidence for the role of TTf in iron acquisition in Dunaliella. In this report, we characterized the iron binding activity of solubilized TTf, purified from plasma membrane vesicles of iron‐starved cells. The characteristics of iron binding to TTf generally resembled those of animal transferrins (Tfs) in an obligatory requirement for bicarbonate as a co‐ligand, specificity for ferric ions and pH dependence of both binding and release. Unlike animal Tfs, Fe binding activity of TTf was stimulated by high NaCl concentrations. The stimulation was not associated with changes in Fe binding affinity. The results unequivocally confirm the role of TTf in Fe binding and uptake in Dunaliella and demonstrate its uniqueness as a membrane‐associated, salt‐stimulated transferrin.  相似文献   

盐藻中叶绿素的提取方法比较及条件优化     

杨代宇  穆富香  白飞妮  于媛  杨海波 《安徽农业科学》2016,44(25)

[目的]确定有效提取盐藻中叶绿素的提取方法并优化提取条件。[方法]在单因素考察乙醇提取盐藻叶绿素优化条件的基础上,通过正交试验确定乙醇提取盐藻叶绿素的最优条件。[结果]乙醇提取盐藻中叶绿素的最优条件为85%乙醇、提取时间12 min、提取温度40℃,总叶绿素的最高提取率为10.80 mg/g湿重,比丙酮提取盐藻中的叶绿素提高了15.5%。[结论]以乙醇为提取剂可从盐藻中提取更多的叶绿素,且安全、无污染、时间短。此结果可为利用乙醇提取和测定海生盐藻中的叶绿素含量或工业生产提取叶绿素提供试验依据和参考。  相似文献   

盐藻粉对奶牛产奶性能与血清免疫球蛋白的影响     

赵鹏  高义彪  索媛  闫素梅  田丰 《畜牧与饲料科学》2011,(Z1):91-93

为了探讨盐藻粉对奶牛产奶性能免疫机能的影响,试验选择处于泌乳中期的健康荷斯坦奶牛18头分为对照组、1.5倍VA(165IU/kg)组和盐藻粉组进行饲喂试验。结果表明,日粮中添加盐藻粉或1.5倍VA对奶牛的产奶性能有一定促进效果,盐藻粉组优于1.5倍VA组;添加盐藻粉可显著降低乳中体细胞数,血清IgG抗体和IgM抗体浓度也有明显增加的趋势;添加1.5倍VA也可显著降低乳中体细胞数,血清IgG抗体浓度有明显升高的趋势。  相似文献   

盐藻粉对奶牛生产性能的影响研究     

马吉锋  常明阳  黎玉琼  薛伟  梁小军 《安徽农业科学》2013,41(3):1126-1127

[目的]为盐藻粉在反刍动物中的应用提供理论依据。[方法]选择40头中国荷斯坦奶牛随机分为4组,分为对照组(基础日粮)、试验Ⅰ组、试验Ⅱ组及试验Ⅲ组,分别在基础日粮的基础上添加4.5、9和13.5 g/d盐藻粉,研究其对奶牛生产性能的影响。[结果]试验Ⅰ组、试验Ⅱ组、试验Ⅲ组奶牛产奶量比对照组分别提高了2.98%、5.24%和6.59%,但差异均不显著(P>0.05);试验Ⅰ组、试验Ⅱ组、试验Ⅲ组乳脂率、乳蛋白、乳糖、非脂乳固形物含量与对照组差异均不显著(P>0.05);3个试验组牛奶中的体细胞数分别比对照组降低了15.7%、29.4%和33.5%。[结论]添加盐藻粉有提高奶牛产奶量、降低牛奶中的体细胞数和改善乳品质量的趋势。  相似文献   

Effects of β-carotene source, Dunaliella salina, and astaxanthin on pigmentation, growth, survival and health of Penaeus monodon   总被引：1，自引：0，他引：1  

M Boonyaratpalin  S Thongrod  K Supamattaya  G Britton  & L E Schlipalius 《Aquaculture Research》2001,32(S1):182-190

  相似文献   

电动多旋翼植保无人机低容量喷雾防治玉米三点斑叶蝉的应用研究   总被引：1，自引：0，他引：1  

赵冰梅  丁丽丽  张强  董红强 《植物保护》2018,44(1):186-189

利用KT-10-Ⅱ型四旋翼植保无人机开展玉米灌浆期三点斑叶蝉的防治试验,研究无人机飞行高度、飞行速度、施药液量以及喷雾助剂等因素对农药雾滴在玉米冠层的沉积分布影响及对三点斑叶蝉防效的影响。结果表明:在无人机飞行高度为距玉米株冠顶部1m、飞行速度6m/s、施药液量15L/hm2,并加入1.5%喷雾助剂"迈飞"的条件下,喷雾雾滴在玉米冠层上部、中部和下部的沉积密度分别达到41.9、27.3和14.9个/cm2,对三点斑叶蝉药后1d防效可达91.3%,药后14d防效96.8%以上,速效性和持效性均较理想。  相似文献   

The Potential Use of Microalgal Carotenoids as Dietary Supplements and Natural Preservative Ingredients     

Hanaa H. Abd El-Baky  Gamal S. El-Baroty 《Journal Of Aquatic Food Product Technology》2013,22(4):392-406

Dunaliella salina has been selected as the most valuable microalgae for production of carotenoids as a source of natural pigment when grown under defined conditions. Compared with optimal conditions, D. salina contained higher amounts of total carotenoids (TCAR, 12.6%, dw)—including β-carotene (60.4%, of total carotenoids), astaxanthin (17.7%), zeaxanthin (13.4%), lutein (4.6%), and cryptoxanthin (3.9%)—when grown under stress conditions. These TCAR showed greater in vitro antioxidant potential by inhibition of lipid peroxidation of rat liver microsomes induced by Fe⁺⁺/ascorbate and Fe⁺⁺/H₂O₂ model systems than butylated hydroxytoluene (BHT). The toxicological evaluation of D. salina functional ingredients as an antioxidant and natural food colorant was conducted, in which male albino rats were fed ad libitum for 6 weeks with one of the following diets: standard diet (S), S diets containing 0.5 g trans β-carotene/kg diets (S + 0.5 β C), S + 0.5 g D. salina carotenoids/kg diets (S + 0.5 DC), and S + 1.0 g D. salina carotenoids/kg diets (S + 1.0 DC). Generally, no mortality or treatment-related toxicological signs were observed in any of the rats during the 6 weeks of the experiment. The concentrations of glucose and glycogen in liver and glucose, total proteins, bilirubin, and creatinine as well as enzymes activities of aspartate (AST) and alanine aminotransferases (ALT), and alkaline phosphatase (ALP) in plasma were not significantly different from each other in all four diets. In contrast, the levels of total lipids and lipids components showed significant differences in all groups. Groups S + 0.5 βC, S + 0.5 DC, and S + 1.0 DC showed a significant decrease in concentrations of total lipids, triglycerides, total cholesterol, and phospholipids in either plasma or hepatic tissues compared with S group. The results showed that D. salina carotenoids could be potentially rich sources of natural antioxidants and food colorants and have hypolipidemic and hypocholesterolemic properties.  相似文献   

巴夫杜氏藻GAPDH基因部分序列的克隆     

尚常花  朱顺妮  袁振宏  王忠铭 《广东农业科学》2011,38(13):126-127,134

3-磷酸甘油醛脱氢酶(GAPDH)是糖酵解途径中的一个关键酶,由于其基因表达量相对恒定.因此在定量和半定量PCR试验中常被用作内源参照基因来确定目标基因的相对表达量.从巴夫杜氏藻(Dunaliella pmva)中克隆GA PDH基因,并对基因序列进行了分析.以巴夫杜氏藻cDNA为模板,根据GAPDH的保守序列设计引物...  相似文献