哈氏弧菌诱导布氏石斑鱼头肾提取物的抗菌活性研究     

陈诏  王世锋  谢珍玉  于文媛  周永灿 《热带生物学报》2010,1(2):165-169

以致病性哈氏弧菌（Vibrio harveyi）腹腔注射布氏石斑鱼（Epinephelus bleekeri）18h后,取布氏石斑鱼头肾制备头肾粗提物,并对该粗提物的抗菌活性进行研究,结果表明：布氏石斑鱼经哈氏弧菌诱导后,其头肾粗提物不仅对金黄色葡萄球菌（Staphyloccocus aureus）等革兰氏阳性菌有良好抑制作用,而且对大肠杆菌D31（Escherichio coli D31）、哈氏弧菌和溶藻弧菌（V.alginolyticus）等革兰氏阴性菌也有良好抑制效果;利用电泳凝胶琼脂糖弥散法检测发现,头肾粗提物中具有多种对大肠杆菌D31有较强作用效果的蛋白类抗菌物质;切胶回收AU—PAGE凝胶上的抗菌组分,利用SDS—PAGE凝胶检测各抗菌活性组分的分子质量大小依次约为：10．9KD,10．0KD,8．1KD,7．2KD,6．3KD,由于这些抗菌活性成分可被蛋白酶破坏,且分子质量均接近或低于10KD,可初步将其认定为抗菌肽。  相似文献   

饥饿对点带石斑鱼饵料转换期仔鱼生长和发育的影响   总被引：10，自引：3，他引：7  

柳敏海  施兆鸿  陈波  罗海忠  傅荣兵  罗海军 《海洋渔业》2006,28(4):292-298

在盐度29、水温27.8~28.8℃的条件下,进行点带石斑鱼仔鱼饥饿实验,确定仔鱼初次摄食饥饿不可逆点(PNR);观察饥饿条件下点带石斑鱼饵料转换期(初孵仔鱼,5、8、10、15和20日龄)的存活与生长、油球利用、鳍棘生长和游泳行为的变化。结果表明:点带石斑鱼从初次摄食到PNR期仅2~3d,这个耐受饥饿的时间临界点发生在孵化后的第5天,即转入外源性营养的第3天。在饥饿条件下初孵仔鱼,5、8、10、15和20日龄仔鱼的全部死亡时间分别为7 d、3 d、4 d、3 d、4 d和6 d;半数死亡时间分别为5 d、1.5 d、2 d、1.5 d、2d和3.5d,说明5日龄和10日龄是点带石斑鱼早期发育中最为敏感的阶段。饥饿会延缓初孵仔鱼对油球的利用。随着饥饿时间的延长,仔鱼的生长与正常条件下仔鱼的生长差异显著(P<0.05)。饥饿仔鱼体长较短,头大且体瘦,长期饥饿后脑后部下陷;行为变化表现为表层集群游动觅食,游动缓慢,反应迟钝和静伏底。  相似文献   

复方中草药及其与抗生素联用对斜带石斑鱼溶藻弧菌病的治疗效果     

徐晓津  李秀华  马一帆  李慧耀  祁欣  郑江  吕海龙  鄢庆枇 《水产学报》2019,43(5):1338-1346

通过药物体外抑菌效果及对斜带石斑鱼抗病力影响的研究,筛选有效治疗斜带石斑鱼溶藻弧菌病复方中草药与中西药联用配方。用诃子、白芍、甘草比例分别为0.9∶1.3∶0.9、1.2∶0.9∶1.0和1∶1∶1的复方中草药对溶藻弧菌进行体外抑菌实验;将质量分数为2.2%的复方中草药与中西药联用添加到基础饲料中设为实验组,研究中西药对斜带石斑鱼溶藻弧菌病治疗效果。复方中草药实验组Ⅰ、Ⅱ、Ⅲ为投喂饲料中添加不同比例诃子、白芍、甘草,中西药联用实验组Ⅳ、Ⅴ、Ⅵ分别为投喂饲料中添加不同比例诃子、白芍、甘草、恩诺沙星。结果显示,复方中草药配方二药物质量浓度200 mg/mL,为最佳配方。40 d病鱼死亡数量比较结果为对照组Ⅱ对照组Ⅳ对照组Ⅲ实验组Ⅲ实验组Ⅱ实验组Ⅰ实验组Ⅴ实验组Ⅵ实验组Ⅳ对照组Ⅰ。实验组Ⅰ投喂饲料加诃子、白芍、甘草比例为0.9∶1.3∶0.9的复方中草药,实验组Ⅳ配方为诃子、白芍、甘草、恩诺沙星比例为0.9∶1.3∶0.9∶1.3,分别为治疗斜带石斑鱼溶藻弧菌病效果最好的复方中草药和中西药联用配方。研究表明,中西药联用复方药效强于复方中草药,均能较好治疗斜带石斑鱼溶藻弧菌病。  相似文献   

日本东京湾云纹石斑鱼染色体核型研究          下载免费PDF全文

钟声平  陈超  庄志猛 《渔业科学进展》2018,39(5):44-49

采用PHA体内直接注射法制备了云纹石斑鱼(Epinephelus moara TemminckSchlegel1842)东京湾群体头肾组织染色体标本并分析其核型。结果显示,其东京湾群体二倍体染色体数为48,其中,亚中部着丝粒3对;其余21对染色体为端部着丝粒染色体,其染色体臂数NF为54,核型公式为:2n=48,6sm+42t,NF=54;第24对染色体的短臂的形态具有多态性。该鱼染色体臂数高于石斑鱼类的原始核型类群,属于石斑鱼属的特化类群。云纹石斑鱼日本东京湾群体与厦门群体染色体核型存在显著差异,东京湾群体比厦门群体多1对亚中部着丝粒染色体,可能是长期隔离导致染色体重组进化形成的。结果表明,云纹石斑鱼不同地理群体间存在丰富的遗传差异,可利用不同地理群体间的种内杂交优势改良云纹石斑鱼种质。  相似文献   

七带石斑鱼染色体核型研究   总被引：6，自引：2，他引：4  

钟声平  陈超  王军  刘志鸿  柳淑芳  朱玲  庄志猛 《中国水产科学》2010,17(1)

采用PHA体内直接注射法制备了七带石斑鱼(Epinephelus septemfasciatus)头肾组织染色体标本并分析其核型。结果表明,七带石斑鱼二倍体染色体数为48,24对染色体均为端部着丝粒染色体,其染色体臂数(NF)为48,核型公式为:2n=48t,NF=48。未发现与性别相关的异型染色体,第24对染色体长度明显小于其他染色体。通过与其他22种石斑鱼染色体核型进行比较,发现七带石斑鱼具有石斑鱼属鱼类的原始核型特征,属于石斑鱼属鱼类的原始类群。  相似文献   

生态清洁型封闭式循环水养鱼技术研究     

王志敏  刘艳芳 《河北渔业》2009,(12):6-10

从预防病害发生入手,应用沉淀分离、旋流分离和砂滤等固液分离技术,提高循环水量,增加固液分离、生物过滤、紫外线消毒等水质净化频次,保持鱼类、水质环境和病原微生物的生态平衡,以生态清洁的方式健康养殖。结果表明,在生态清洁型循环水系统中养殖点带石斑鱼,养殖期12个月,无明显病害发生,养殖过程不用药,养殖产量为25.44 kg/m2,成活率87.42%。  相似文献   

Neutralizing antibody levels for protection against betanodavirus infection in sevenband grouper, Epinephelus septemfasciatus (Thunberg), immunized with an inactivated virus vaccine     

H Yamashita  K Mori  A Kuroda  T Nakai 《Journal of fish diseases》2009,32(9):767-775

An inactivated betanodavirus, red‐spotted grouper nervous necrosis virus (RGNNV), is a vaccine candidate for viral nervous necrosis (VNN). The present study was conducted to examine inoculation doses of the vaccine and neutralizing antibody titre levels to protect fish against VNN. Young sevenband grouper, Epinephelus septemfasciatus, averaging 25.4 g, were immunized at 25 °C water temperature by a single intraperitoneal injection of formalin‐inactivated RGNNV. Fish immunized at vaccine doses of 10^8.5, 10^8.0, 10^7.5, 10^7.0 and 10^6.5 TCID₅₀ per fish produced antibodies at mean titres of 1:907, 1:511, 1:259, 1:197 and 1:96, respectively, at 20 days post‐immunization (p.i.). Neutralizing antibodies were not detected in any control fish (titre <1:80). When fish were challenged with RGNNV (10^5.0 and 10^4.0 TCID₅₀/fish) at 20 days p.i., cumulative mortalities of the fish groups immunized with 10^8.5, 10^8.0, 10^7.5 and 10^7.0 TCID₅₀ per fish were significantly lower than those of the control group, and the relative percent survival values were higher than 60% in fish groups immunized with 10^7.5 TCID₅₀ per fish or higher doses. However, no significant differences were found in mortality between the group immunized with 10^6.5 TCID₅₀ per fish and the control group. From these results, it was deduced that the minimum effective inoculation dose of the vaccine is 10^7.0 TCID₅₀ per fish and the minimum mean neutralizing antibody titre giving significant protection is approximately 1:200. This antibody titre level is a possible measure of vaccine efficacy against VNN in sevenband grouper, instead of a virus challenge test.  相似文献   

Fisheries replenishment of early life taxa: potential export of fish eggs and larvae from a temperate marine protected area     

R. CREC’HRIOU  F. ALEMANY  E. ROUSSEL  A. CHASSANITE  J. Y. MARINARO  J. MADER  E. ROCHEL  S. PLANES 《Fisheries Oceanography》2010,19(2):135-150

An ichthyoplankton survey was conducted at the periphery of Cabrera National Park (Balearic Islands, Western Mediterranean Sea) in July 2004, using bongo nets, fixed nets and collection of oceanographic data. This work focused on analysing the distribution of eggs and larval stages of some fishery‐targeted taxa (Coris julis, Epinephelus spp., Sciaena umbra and Scorpaena sp.) whose adult abundances and/or biomass are higher in the reserve and therefore would be likely to show gradients of larval abundance attributable to the existence of the Marine Protected Area (MPA). Oceanographic data indicated there was water column stratification, horizontal distribution of surface water masses and hydrodynamic features linked with Mediterranean seasonality. During the summer sampling, eggs and larvae of targeted fish taxa were mainly located in the northeast of the MPA, near the coast. An effect of depth and current was demonstrated for Coris julis and Epinephelus spp. and there appears to be an offshore gradient for the other targeted taxa, especially for the egg stages. These data highlight the fact that the Cabrera archipelago is a potential important spawning area for targeted fishery species.  相似文献   

Parasites of cultured and wild brown‐marbled grouper Epinephelus fuscoguttatus (Forsskål, 1775) in Lampung Bay,Indonesia     

Sonja Rückert  Sven Klimpel  Harry Wilhelm Palm 《Aquaculture Research》2010,41(8):1158-1169

A total of 210 Epinephelus fuscoguttatus (brown‐marbled grouper) was examined for parasites. During three consecutive seasons (two rainy and one dry season from 2002 to 2004), 35 specimens each taken from floating net cages of the National Sea Farming Development Centre (Balai Budidaya Laut) and from wild catches in Lampung Bay, South Sumatra, Indonesia were studied. Twenty‐five (cultured grouper) and 30 (wild grouper) parasite species/taxa were identified, with an infracommunity ranging from one to nine (cultured) and three to 14 parasite species (wild), demonstrating a species‐rich parasite fauna even in the cultured fish. Protozoans (1 species), microsporeans (1), myxozoans (1), digeneans (8), monogeneans (5), cestodes (3), nematodes (8), acanthocephalans (2) and crustaceans (6) were found. The most abundant parasites were the monogeneans Pseudorhabdosynochus epinepheli and Pseudorhabdosynochus lantauensis for both, cultured and wild grouper during all seasons. For the cultured fish, the prevalence of monoxenous ectoparasites (e.g. P. epinepheli, P. lantauensis, Capsalidae gen. et sp. indet., Benedenia epinepheli) was in most cases higher than that of heteroxenous endoparasites. This contrasts the wild grouper, where heteroxenous parasites such as Allopodocotyle epinepheli and Raphidascaris sp. occurred at a similar prevalence compared with the fairly abundant Pseudorhabdosynochus spp. No seasonality of infestation was observed for both cultured and wild fish. The high levels of infestation of potentially pathogenic monogeneans throughout the year could result in significant parasite outbreaks at the locality studied.  相似文献   

低盐度胁迫对点带石斑鱼幼鱼消化酶、抗应激酶和存活率的影响   总被引：4，自引：1，他引：3       下载免费PDF全文

余燕  徐维娜  刘兆普  刘文斌 《渔业科学进展》2009,30(4):21-26

探讨了低盐度胁迫对点带石斑鱼幼鱼消化酶、抗应激酶及幼鱼存活率影响。实验设计了28．23、22．56、17．57、12．67和7．71等5个盐度梯度,实验鱼共40尾（体重20±2．32g）,先放入盐度为28．23的水体中驯化10d,然后按以上梯度逐级淡化,在每个盐度梯度维持3d后取样,测定消化酶和抗应激酶等指标,并观察其活动和存活状况。结果表明,胃和肠_的蛋白酶、淀粉酶活力随盐度梯度的下降显著降低（P〈0．05）;肝脏、肌肉和肾脏的SOD活力随盐度梯度下降呈上升趋势,肝脏、肌肉和肾脏的CAT和GSH—Px活力随盐度梯度下降呈降低趋势;鱼的活动能力从盐度梯度为17．57时开始下降,12．67时即出现幼鱼死亡现象,盐度梯度下降到7．71时,幼鱼在12h内全部死亡。说明低盐度胁迫将会严重影响点带石斑鱼幼鱼消化能力并导致鱼体自由基代谢紊乱,应激增强,最终将影响幼鱼健康生长。  相似文献