封闭式循环水饲养青石斑鱼试验   总被引：2，自引：0，他引：2  

朱庆国 《福建水产》2006,(1):36-38

在封闭式循环水养殖系统中以全人工配合饲料制成软颗粒饲料投喂青石斑鱼幼鱼,经过33d的饲养,青石斑鱼尾相对增重率为64.6%,平均饲料系数为1.81,养殖成活率95.89%,平均丰满度为1.66。封闭式循环水养殖系统的养殖排出水回收率达到85%以上,养殖单产、饲料效率、生长速度都达到了生产要求。  相似文献   

What causes cannibalization‐associated suffocation in cultured brown‐marbled grouper,Epinephelus fuscoguttatus (Forsskål, 1775)?     

Jinn-Rong Hseu  Wen-Bin Huang  & Yeong-Torng Chu 《Aquaculture Research》2007,38(10):1056-1060

During culture of grouper, cannibalism is a frequent phenomenon that usually causes economic loss. Grouper culture often requires grading to prevent size‐dependent cannibalism. In comparison with orange‐spotted (Epinephelus coioides) and giant grouper (E. lanceolatus), failure to swallow prey during cannibalism is frequently observed in brown‐marbled grouper (E. fuscoguttatus). The cannibal cannot engulf the entire prey and the two fish ultimately end up dying together. Herein, we attempted to compare morphometric differences among orange‐spotted, giant and brown‐marbled grouper. We established a cannibal–prey total length (TL) relationship for brown‐marbled grouper: TL_prey=0.71 TL_cannibal−1.75. According to the equation, a difference of approximately 50% in TL is a threshold to use for grading the grouper. This value is much larger than that used (30%) in orange‐spotted and giant grouper, and this size difference results in a higher incidence of failed cannibalism attempts in brown‐marbled grouper. It is our belief that the standard grading method will fail to produce as good an effect in brown‐marbled grouper as is seen in orange‐spotted and giant grouper. Therefore, in addition to grading, other manipulations such as satiation feeding, nutritional supplementation and optimal stocking densities should be applied to reduce cannibalism of brown‐marbled grouper.  相似文献   

赤点石斑鱼仔稚幼鱼的形态发育和生长     

王涵生  方琼珊等 《上海海洋大学学报》2001,(4):307-312

通过赤点石斑鱼各个发育期的仔稚幼鱼连续取样,系统地观察了仔稚幼鱼各个发育期的形态变化及生长特征,并进行描图。赤点石斑鱼初孵仔鱼全长1．35－1．45mm。稚鱼第6－7d长出特别伸长的背鳍第2鳍棘和腹鳍棘,其长度与全长之比在第16－18d,全长11mm左右时达到最大值,接近50%,这一形态特点是石斑鱼属仔稚鱼期特有的。以后随着个体的生长和其他鳍棘的伸长,这一比例逐渐减小,直至小于其他鳍棘,这是稚鱼完成变态成为鱼种的重要标志。最早完成变态的稚鱼须在孵化后第35 d,全长30mm以上,但到第45d时,全长24mm的个体也可以完成变态。即使在相同的环境条件下,仔稚幼鱼的个体大小差异也很大,且越到后期差异越大。  相似文献   

Pyridoxine deficiency of grouper, <Emphasis Type="Italic">Epinephelus coioides</Emphasis>: physiological and biochemical alteration     

J.?W.?Huang  L.?X.?Tian  Z.?Y.?Du  H.?J.?Yang  Y.?J.?LiuEmail author 《Fish physiology and biochemistry》2005,31(4):331-337

Pyridoxine is essential for animals. The purpose of this study was to investigate the pyridoxine deficiency symptoms and the fit dose of pyridoxine to keep normal physiological function in grouper, Epinephelus coioides. Graded levels of pyridoxine (0, 1, 2, 4, 8, 16, 32 mg pyridoxine kg⁻¹ diet) were fed to grouper juveniles (14.83±0.31 g) for 8 weeks, and then some physiological and biochemical parameters were measured. Pyridoxine deficiency symptoms such as anorexia, poor survival, convulsion, helically swimming and hyperirritability were observed in fish fed the pyridoxine-deficient diet, and these fish also showed low whole blood haemoglobin level, liver glutamic-pyruvic transaminase (GPT) and superoxide dismutase (SOD) activities. Slight symptoms of pyridoxine deficiency also appeared among fish fed the diet with very low dose (1 mg pyridoxine kg⁻¹ diet) of pyridoxine in 6 weeks. Broken-line regression analysis showed that the optimal pyridoxine levels for these fish to keep normal survival, whole blood haemoglobin level, liver GPT and SOD activities were approximately 1.75, 1.87, 2.22 and 2.05 mg kg⁻¹, respectively.  相似文献   

Effect of dietary lipid level on growth performance,feed utilization and body composition of grouper <Emphasis Type="Italic">Epinephelus coioides</Emphasis> juveniles fed isonitrogenous diets in floating netcages     

ZHI?LUO  Yong-Jian?LiuEmail author  Kang-Sen?Mai  Li-Xia?Tian  Dong-Hui?Liu  Xiao-Ying?Tan  Hei-Zhao?Lin 《Aquaculture International》2005,13(3):257-269

An experiment was conducted to determine the effect of dietary lipid levels on growth performance and body composition of grouper Epinephelus coioides juveniles cultured in floating netcages (1.5 m × 1 m × 1.5 m). Six isonitrogenous diets (53% dietary protein) with increasing dietary lipid concentration (5.16, 7.38, 9.73, 12.36, 14.13, 16.04% of dry material, DM) were fed to satiation to triplicate groups of 20 fish (mean weight: 10.9 ± 0.1 g) for 56 days. Dietary energy levels were 300, 322, 340, 371, 382, 400 kcal/100 g DM, respectively. Fish fed the 9L diet had the highest weight gain (WG) and specific growth rate (SGR), but they were not significantly different from that of fish fed the 7L or 12L diet (p > 0.05). FI varied inversely with dietary lipid levels. The poorest FCR and the lowest PER were observed in fish fed the 5L diet but these parameters showed no significant differences (p > 0.05) for fish fed the dietary lipid level ranging from 9 to 16%. Nitrogen intake decreased with dietary lipid levels. Fish fed the 7L diet showed the highest N gain, which was not markedly different from that of fish fed the 9L and 12L diets (p > 0.05). N retention was significantly lower in fish fed the 5L diet than in the other groups. Lipid intake and lipid gain increased with dietary lipid levels. Lipid retention (%) of the 7L diet was lowest but showed no significant differences among other treatments (p > 0.05). Proximate composition of fish body was significantly affected by dietary lipid level (p < 0.05). Body lipid content increased in direct proportion to dietary lipid levels. In contrast, moisture content of whole-body was correlated negatively to dietary lipid level. Dietary lipid content had no significant effect on protein content in whole-body and white muscle (p > 0.05). Condition factor (CF), hepatosomatic index (HSI) and viscerosomatic index (VSI) increased with increasing dietary lipid level. Based on second-order polynomial regression analysis of WG against dietary lipid level, a breakpoint of 10.0% was indicated to be the optimal dietary lipid concentration for maximum growth for grouper Epinephelus coioides juveniles cultured in floating netcages.  相似文献   

Apparent digestibility of selected ingredients in diets for juvenile grouper, Epinephelus coioides (Hamilton)     

Perla S Eusebio  Relicardo M Coloso  & Roger E P Mamauag 《Aquaculture Research》2004,35(13):1261-1269

Apparent digestibility coefficients (ADCs) for dry matter (ADC_dm) and crude protein (ADC_cp) of selected feed ingredients were determined in vivo for grouper using passive faeces collection (Guelph System). A reference diet (RF) and test diets (consisted of 70% RF and 30% test ingredient) with 1% Cr₂O₃ as an inert indicator were used. An RF contained 45% protein, 10% fat and 15.7 kJ g^?1 metabolizable energy. Three isonitrogenous and isocaloric diets, each contained a test ingredient (white fish meal, white cowpea meal and ipil‐ipil leaf meal), were used in a growth study based on ADC_cp of feed ingredients. An RF without Cr₂O₃ was a control. The ADC values of experimental diets were also determined. In grouper, the ADC_dm of white cowpea meal, defatted soybean meal, wheat flour and shrimp meal (74–76%) were significantly lower than that of squid meal (99%), but comparable with those of the fish meals (84–89%). No significant difference was observed between the ADC_dm of ipil‐ipil leaf meal, rice bran and wheat flour (56–73%). The ADC_cp of white cowpea meal and defatted soybean meal were similar to those of the fish meals, squid meal and shrimp meal (94–99%). The ADC_cp of wheat flour was comparable with that of ipil‐ipil leaf meal (79–83%). Rice bran had the lowest ADC_cp value of 43%. Based on specific growth rate (SGR), the growth of fish fed white cowpea meal‐based diets was similar to that of the control fish (3.2–3.3% day^?1). Also, no significant difference was observed between the ADC_dm (68–72%) and ADC_cp (88–91%) of white cowpea meal‐based diet and the control diet. The results suggest that ADC values can be used as indicators to determine the nutritional value of feed ingredients. White cowpea meal can be incorporated as a protein source in practical diet for grouper at 20.5% of the diet with no adverse effect on growth.  相似文献   

Establishment of cell lines from a tropical grouper,Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper irido- and nodaviruses   总被引：1，自引：0，他引：1  

Lai YS  John JA  Lin CH  Guo IC  Chen SC  Fang K  Lin CH  Chang CY 《Journal of fish diseases》2003,26(1):31-42

Four tropical marine fish cell lines have been established from the eye, fin, heart and swim bladder of grouper, Epinephelus awoara (Temminck & Schlegel). Optimum media and temperature conditions for maximum growth were standardized. The eye and swim bladder cells were mostly epithelial, but the fin and heart cells were mostly fibroblastic. The viability of cells was 95% after 1 year of storage in liquid nitrogen (-196 degrees C). Besides these four cell lines, previously established grouper brain, kidney and liver cell lines were also used for a viral susceptibility study which showed that all the cell lines were sensitive to grouper iridovirus, whereas only brain, fin and liver cell lines were susceptible to the yellow grouper nervous necrosis virus (a nodavirus). Electron microscopy studies of the grouper irido- and nodaviruses in ultrathin sections of infected cells showed an abundance of viral particles in the cytoplasm of the virus-infected cells indicating the effective replication of these two viruses. It is suggested that these cell lines can be used for the isolation of putative fish specific viruses and provide a valuable tool to study the mechanisms of host-pathogen interactions. Furthermore, these cell lines upon transfection, using pEGFP-C1 and pEGFP-aMT2.5 (ayu metallothionein promoter), produced significant fluorescent signals indicating their utility for exogenous studies.  相似文献   

龙胆石斑鱼集约化养殖试验     

肖懿哲 《水产科技》2006,(6)

从2002年12月19日引进3～5cm的龙胆石斑鱼苗820尾,经过11个月养殖试验测定,共存活483条,成活率58.9%,平均体重473g/尾,最大体重950g/尾,养殖期间,饵料系数1.44,饵料效率69%,养殖总积温7900℃。  相似文献   

斜带石斑白细胞cDNA文库的构建   总被引：5，自引：2，他引：5  

殷志新 《水产学报》2001,25(6):538-541

用Tripure试剂盒提取斜带石斑白细胞总RNA,反转录合成第一链cDNA,进行长距离PCR,蛋白酶K消化,Sfi I酶切,通过CHROMASPIN-400柱,回收大于500bp的cDNA,与λTripIEx2载体连接,体外包装后构建了斜带石斑白细胞的cDNA文库.库容1.65×1.06,重组频率82%,扩增后滴度7.5×109pfu·mL-1.插入片断长度500～2300bp,最多的是在750～1000bp范围.本文库可作为筛选斜带石斑细胞因子基因的重要资源.  相似文献   

foxl3重组质粒过表达与17ɑ-甲基睾酮投喂对斜带石斑鱼性腺发育的影响     

张春丽  何琪  程慧涛  李丽华  阮炘贺  段旭琢  黄枫淇  杨慧荣  张海发  石和荣  王庆  赵会宏 《水产学报》2022,46(12):2286-2296

通过foxl3重组质粒过表达与MT投喂对比,探究一种新型的调控斜带石斑鱼性腺发育的方法。以foxl3重组质粒注射以及MT投喂3月龄的石斑鱼幼鱼,观察性腺发育状态,利用荧光定量PCR检测分析性腺分化相关基因的表达。结果显示：质粒注射初期性腺注射组卵巢较对照组小,体细胞较多,存在向雄性方向发育的趋势（存在睾丸间质细胞）,且质粒注射初期的雄性相关基因—amh,激素生成相关基因—cyp11b、11β-hsd2,精子发生相关基因—rec8和sycp3的相对表达量显著高于对照组。MT投喂组后期的性腺组织处于明显的兼性发育期,而对照组仍处于O2期卵母细胞期;MT投喂组,amh、cyp11b、11β-hsd2、rec8和sycp3基因相对表达量趋势与质粒注射一致,但投喂组与对照组之间的差异较质粒注射更显著。在本实验条件下,MT投喂调控性腺雄性化的效果较foxl3质粒注射的效果明显。以上结果表明,foxl3重组质粒过表达对斜带石斑鱼幼鱼性腺发育具有明确的雄性化调控作用,且这种调控可能具有剂量依存效应。  相似文献