Effect of Lactococcus lactis and Enterococcus faecium on growth performance,digestive enzymes and immune response of grouper Epinephelus coioides     

Y.‐Z. SUN  H.‐L. YANG  R.‐L. MA  K. SONG  J.‐S. LI 《Aquaculture Nutrition》2012,18(3):281-289

The effect of dietary administration of Lactococcus lactis MM1 and Enterococcus faecium MM4, two strains of lactic acid bacteria isolated from the gut of juvenile grouper Epinephelus coioides, on the growth, feed utilization, digestive enzymes and immune response of juvenile E. coioides was evaluated. The fish were fed for 60 days with three different diets: control diet (without probiotic), diet P1 supplemented with 1.0 × 10⁸ CFU g^?1L. lactis or diet P2 with 1.0 × 10⁸ CFU g^?1E. faecium. Although growth and feed intake decreased slightly, significant improvement of feed utilization was observed in fish fed diet P1 and diet P2 for 60 days. The hepatopancreatic protease activities in the two probiotic treatments were significantly higher than that in the control. The phagocytic activity, serum superoxide dismutase activity and complement component 4 (C4) and IgM levels were not significantly affected by diet P1 and diet P2. Serum complement component 3 (C3) levels in the two treatments were significantly higher than that in the control. Significant increased serum lysozyme activity was observed in fish fed the diet P1, while significant decreased lysozyme activity was observed in fish fed the diet P2 compared with the fish fed the control diet. There was a significant interaction effect between diet and administration date for the complement C4. The study demonstrates potential for L. lactis MM1 and E. faecium MM4 to improve the feed utilization and modulate the immune function in E. coioides.  相似文献   

口服当归多糖对点带石斑鱼非特异性免疫力的影响   总被引：1，自引：0，他引：1  

王庆奎  赵海运  吕志敏  陈成勋  邢克智 《安徽农业科学》2011,39(22):13857-13860

[目的]研究口服当归多糖对点带石斑鱼非特异性免疫力的影响。[方法]将05、02、00、350和500 mg/kg当归多糖添加到基础饵料中饲喂点带石斑鱼28 d,研究血浆中酸性磷酸酶（ACP）、溶菌酶（LZM）、补体C3和C4含量、白蛋白/球蛋白比值（A/G）、血液白细胞数和白细胞吞噬能力。[结果]喂食当归多糖能提高血浆ACP、LZM活力、补体C3和C4含量,降低血浆A/G值。另外,喂食当归多糖能提高点带石斑鱼血液白细胞数和白细胞吞噬率。[结论]当归多糖能提高点带石斑鱼非特异性免疫力,饵料中当归多糖的建议添加量为50 mg/kg,连续喂食21 d。  相似文献   

Effects of dietary lipid level on growth,fatty acid composition,digestive enzymes and expression of some lipid metabolism related genes of orange‐spotted grouper larvae (Epinephelus coioides H.)          下载免费PDF全文

Songlin Li  Kangsen Mai  Wei Xu  Yuhui Yuan  Yanjiao Zhang  Huihui Zhou  Qinghui Ai 《Aquaculture Research》2016,47(8):2481-2495

  相似文献   

赤点石斑鱼FAS基因的克隆及序列分析     

陈斯钰  张芮  杨云霞 《安徽农业科学》2016,44(25)

[目的]研究赤点石斑鱼(Epinephelus akaara)FAS基因的结构与表达特性。[方法]以前期获得的转录组序列作为数据库,应用PCR技术,获得赤点石斑鱼FAS基因的c DNA全序列。[结果]该序列与大黄鱼(Larimichthy scrocea)FAS基因的相似度最高,为87%。该序列全长8 505 bp,包括712 bp的3'UTR区、7 548 bp的开放阅读框(ORF)以及245 bp的5'UTR区,可编码2 515个氨基酸。经预测,该蛋白分子量为274.03 ku,等电点为5.98,其属于亲水性蛋白。通过Real-time PCR方法获得FAS在赤点石斑鱼各个组织中的表达,结果显示,其在心脏中表达量最高,其次是在肝脏中。FAS基因的进化与物种进化一致。[结论]该研究为进一步研究赤点石斑鱼脂肪代谢奠定理论基础,也为赤点石斑鱼在养殖方面的工作提供基础资料。  相似文献   

投喂频率对流水养殖鞍带石斑鱼生长、摄食及免疫酶活力的影响          下载免费PDF全文

仇登高  郑乐云  黄种持  吴水清  朱志煌  刘银华  吴精灵  邱峰岩 《西南大学学报(自然科学版)》2018,40(7):51-57

在水温(28.59±1.74)℃下,选用冰鲜杂鱼饲养平均体质量为858.73g的鞍带石斑鱼,经过60d的生长实验,研究了不同投喂频率(1次/d、1次/2d、1次/3d)对流水养殖鞍带石斑鱼生长、摄食及免疫酶活力的影响.结果表明,投喂频率对鞍带石斑鱼存活率的影响差异不具有统计学意义(p0.05),实验期间各个实验组存活率在99.40%~99.80%之间;各实验组间的肥满度(CF=2.22~2.35)、体质量变异系数(SV=20.08~24.27)、饲料系数(FCR=2.70~3.01)和日增质量(7.50~12.51g/d)均无显著影响(p0.05),而投喂频率1次/d组和1次/2d组的特定生长率(SGR)和相对增质量(RWG)均显著高于1次/3d组(p0.05),投喂频率1次/d组的日摄食率和1次/2d组的饲料转化效率均显著高于1次/3d组(p0.05);在免疫酶活力方面,投喂频率对鞍带石斑鱼血浆中的碱性磷酸酶(AKP=0.083 5~0.089金氏单位/mL)、过氧化氢酶(CAT=7.96~8.19U/mL)及超氧化物歧化酶(SOD=32.40~34.45U/mL)均无显著影响(p0.05).在本实验条件下,流水模式养殖鞍带石斑鱼的适宜投喂频率为1次/d或1次/2d.  相似文献   

Iron bioavailability of different sources in juvenile grouper Epinephelus coioides          下载免费PDF全文

Qin‐Cheng Huang  Er‐Long Wang  Amoah Kwaku  Xiao‐Hui Dong  Bei‐Ping Tan  Shu‐Yan Chi  Qi‐Hui Yang  Shuang Zhang  Hong‐Yu Liu  Yuan‐Zhi Yang 《Aquaculture Research》2018,49(8):2799-2807

A 70‐day experiment was implemented to study the iron (Fe) bioavailability of three Fe sources in Epinephelus coioides (initial weight, 12.19 ± 0.14 g). Treatments consisted of 0, 50, 90, 130, 170 and 210 mg supplemental Fe/kg from iron sulphate (FeSO₄), ferric citrate (Fe‐citrate) and hydroxy methionine analogue iron (Fe‐MHA). The results showed that the growth performance was not affected by Fe level regardless of Fe sources. Hepatic catalase activity (CAT), the haematocrit value (Hct), haemoglobin (Hb), mean corpuscular volume (MCV) and Fe content in whole body or liver firstly increased significantly and then plateaued as Fe level increased regardless of the Fe source, except significant decreasing of Hct in 210 mg Fe/kg group from FeSO₄. Manganese (Mn) content decreased significantly and then kept stable in the whole body and liver whatever the source was. The slope‐ratio method using FeSO₄ as the reference and Hb content, CAT activity, body and hepatic Fe concentration as the response parameter showed the average relative bioavailability of Fe‐citrate and Fe‐MHA was 103.25 ± 4.35 (n = 4) and 134.5 ± 15.67 (n = 4), respectively, with the maximum value of Fe‐MHA in any case. In conclusion, Fe‐MHA was the most effective to meet preferable haematological index, CAT activity and mineral deposition, with little difference between Fe‐citrate and FeSO₄, athough Fe levels with different sources failed to influence the growth in grouper.  相似文献   

鞍带石斑鱼胚胎发育及仔鱼形态发育、饵料转变的观察研究   总被引：2，自引：0，他引：2  

周玲  翁文明  李金亮  赖秋明 《中国农学通报》2010,26(1):293-302

本文对鞍带石斑鱼（Epinephelus lanceolatus）的胚胎发育、仔鱼形态发育及饵料转变进行了观察研究,详细描述了胚胎发育各个时期的形态特征。鞍带石斑鱼受精卵在水温29±0.5 ℃、静水微充气、盐度30的孵化条件下,经过18 h 30 min仔鱼开始孵化出膜。胚胎发育过程分为卵裂期、囊胚期、原肠胚期、神经胚期和器官形成期。在水温25.0~30.0 ℃和盐度33.0 ~35.0的室外水泥池进行初孵仔鱼的培育试验,仔鱼在孵化出膜后72h开口摄食。4日龄仔鱼的卵黄囊几乎完全消失,开始摄食原生动物;从5日龄开始,摄食行为明显,摄食原生动物和小轮虫; 8日龄仔鱼逐渐转向摄食桡足类,兼食少量的轮虫;13日龄后,仔鱼的饵料以桡足类成体为主,摄食量大;经过21天培育进入稚鱼期。  相似文献   

南海斜带石斑鱼野生群体与人工繁殖群体的微卫星分析     

骆剑  黄宗文  陈国华  王珺  杨薇  郭仁湘 《热带生物学报》2011,(2):123-128

为了评估我国斜带石斑鱼野生群体与人工繁殖群体的种质现状,采用19个微卫星标记对斜带石斑鱼南海野生群体和海南人工繁殖群体进行遗传多样性研究。结果显示,在野生和人工繁殖群体中,19个基因座位的有效等位基因数（Ne）分别为1.135 0～4.516 2和1.00 0～3.982 7;群体平均观测杂合度（Ho）分别为0.706 1和0.687 5;群体平均期望杂合度（He）分别为0.480 3和0.421 5;多态信息含量（PIC）分别为0.547 5和0.493 0;2个群体间的遗传相似系数（Gst）为0.979 4,遗传距离（D）为0.020 8。这表明斜带石斑鱼南海野生群体与人工繁殖群体的遗传多样性仍然比较丰富,人工繁殖群体没有出现明显的遗传分化和种质退化的现象。  相似文献   

周期性饥饿对点带石斑鱼摄食和生长的影响     

王庆奎  邢克智  陈成勋  郭永军  张瑞 《安徽农业科学》2010,38(9):4616-4617,4650

[目的]研究周期性饥饿对点带石斑鱼摄食和生长的影响。[方法]对点带石斑鱼幼鱼（初始体重23.97±3.97g）进行周期性饥饿＂饥饿1d喂食5d＂（S1F5）、＂饥饿1d喂食7d＂（S1F7）,对照组持续喂食。各组日投饵量占体重的2%,试验共进行48d。[结果]S1F5组和S1F7组的湿重特定生长率（SGRw）和增重率（WGR）均小于对照组,S1F5组的饵料湿重转化率（FCEw）低于对照组,而S1F7组的FCEw略高于对照组。[结论]周期性饥饿降低点带石斑鱼幼鱼的SGRw和试验结束时体重,而且饥饿频率越高越明显。点带石斑鱼幼鱼在周期性饥饿过程中的生长属不能补偿生长。  相似文献   

致病性假交替单胞菌的分离鉴定及药敏特性研究     

乔迁  韩娜娜  陈超  杨志  史成银  黄偼  李琪 《安徽农业科学》2010,38(26):14224-14226,14232

[目的]分离鉴定患病的七带石斑鱼（Epinephelus septemfasciatus Thunberg）的致病菌,并研究其药敏特性。[方法]从患病的七带石斑鱼体内分离出1株致病菌BY0081。通过ATB微生物鉴定系统和菌体常规形态特征、生理生化反应指标测定以及16S rDNA测序分析等进行综合鉴定;通过人工感染水生模式动物剑尾鱼测定该菌株对其的半数致死量（LD50）;最后进行药敏试验确定其药敏特性。[结果]致病菌BY0081为革兰氏阴性菌,菌落呈米色,边缘光滑,中间略有隆起,为假交替单胞菌（Pseudoa lteromonas sp.）。其在较低的温度下仍有较强的毒力,其对水生模式动物剑尾鱼（Xiphophorus helleri）的LD50为2.82×105cfu/g鱼体重。该菌对菌必治、复方新诺明、氯霉素和氟哌酸等抗生素高度敏感,对复达欣、红霉素、丁胺卡那霉素和庆大霉素等药物中度敏感。[结论]该研究对防治由假交替单胞菌引起的鱼类疾病有积极意义。  相似文献