Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison (Bison bison athabascae)     

Jesus Manuel Palomino  Gabriela F. Mastromonaco  Miriam P. Cervantes  Reuben J. Mapletoft  Muhammad Anzar  Gregg P. Adams 《Reproduction in domestic animals》2020,55(1):54-63

Two experiments were done using a two-by-two design to determine the effects of season and superstimulatory protocol on embryo production in wood bison. In Experiment 1 (in vivo-derived embryos), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with either two or three doses of FSH given every-other-day (FSH × 2 vs. FSH × 3, respectively). Bison were given hCG to induce ovulation, inseminated 12 and 24 hr after hCG, and embryos were collected 8 days after hCG (n = 10 bison/group). In Experiment 2 (in vitro embryo production), ovarian superstimulation was induced in female bison during the ovulatory and anovulatory seasons with two doses of FSH, and in vivo maturation of the cumulus–oocyte complexes (COC) was induced with hCG at either 48 or 72 hr after the last dose of FSH. COC were collected 34 hr after hCG, and expanded COC were used for in vitro fertilization and culture. In Experiment 1, the number of follicles ≥9 mm, the proportion of follicles that ovulated, the number of CL, and the total number of ova/embryos collected did not differ between seasons or treatment groups, but the number of transferable embryos was greater (p < .05) in the ovulatory season. In Experiment 2, no differences were detected between seasons or treatment groups for any end point. The number of transferable embryos produced per bison was greatest (p < .05) using in vitro fertilization and was unaffected by season (1.5 ± 0.2 and 1.1 ± 0.3 during anovulatory and ovulatory seasons, respectively), in contrast to in vivo embryo production which was affected by season (0.1 ± 0.01 and 0.7 ± 0.2 during anovulatory and ovulatory seasons, respectively). Results demonstrate that transferable embryos can be produced throughout the year in wood bison by both in vivo and in vitro techniques, but the efficiency of embryo production of in vivo-derived embryos is significantly lower during the anovulatory season.  相似文献   

猪FSHβ亚基基因的研究进展   总被引：4，自引：0，他引：4  

蒋岸岸  李学伟 《安徽农业科学》2005,33(5):913-914

从生物学作用、基因结构、多态性及其与经济性状的关系方面阐述了被认为是控制猪产仔数候选的基因FSHβ亚基基因研究进展。  相似文献   

光照时间对兴国灰鹅产蛋和PRL、FSHβ、LH mRNA表达的影响     

黄江南  刘林秀  武艳平  康昭风  韦启鹏  周泉勇  季华员  曾文  陈受金  谢金防 《江西农业大学学报》2015,(2):308-313

为研究光照时间对兴国灰鹅产蛋量及产蛋性状相关基因在下丘脑-垂体-性腺轴表达水平的影响,对兴国灰鹅分别进行长光照处理(试验组)和自然光照处理(对照组)。结果发现,整个试验阶段,对照组持续产蛋,产蛋率维持在10%以上,处理组在接受递增式长光照至40 d左右时,产蛋率下降到8%左右,并于试验第47天停产。基因表达水平方面,相对于对照组产蛋期鹅,试验组鹅下丘脑中催乳素(PRL)的mRNA表达水平与输卵管和卵巢中促卵泡素(FSHβ)的表达水平均显著升高,而卵巢中黄体生成素(LH)的mRNA表达水平显著降低。这些结果表明长光照可通过影响PRL的mRNA表达,从而导致FSHβ和LH表达比例失调,最终影响FSHβ和LH的协同作用,从而抑制兴国灰鹅的产蛋活动。  相似文献   

不同激素对卵母细胞体外成熟培养的影响   总被引：1，自引：0，他引：1  

吕丽华  任有蛇  李富忠  岳文斌 《山西农业大学学报(自然科学版)》2009,29(1)

本试验对比在成熟液中添加不同来源的促性腺激素,即进口的和国产的促性腺激素(FSH+LH)对山羊卵母细胞体外培养成熟效果的影响。二者对山羊卵母细胞成熟均有促进作用,卵母细胞成熟率都极显著高于对照组(P<0.01),分别为67.3%、68.9%和15.6%,但是它们之间的成熟率差异不显著(P>0.05)。国产激素成本低,使用方便,在卵母细胞体外成熟培养时,完全可以用国产的促性腺激素代替进口的FSH和LH。  相似文献   

支持细胞中促卵泡素信号通路研究进展   总被引：4，自引：1，他引：3  

高小燕  孙燕  张家骅 《动物医学进展》2006,27(5):1-5

支持细胞在精子生成过程中起重要作用,支持细胞的数量决定睾丸大小、生精细胞数量以及最终生成精子数量。促卵泡素作为保证雄性生育能力的重要激素之一,通过与支持细胞上的促卵泡素受体结合,诱导5种信号途径,导致支持细胞中各类转录因子被激活,引起基因表达发生变化,从而保证生精细胞顺利发育成精子。文章主要围绕支持细胞中促卵泡素信号途径,及其对细胞发育过程中相关基因表达的影响等方面进行了综述。  相似文献   

FSH通过AKT/FOXO1通路调控绵羊卵泡颗粒细胞增殖的研究     

刘春洁  王兆琛  杜炜  赵勇超  张家新 《中国农业大学学报》2020,25(3):45-51

旨在研究FSH-AKT-FOXO1途径调控绵羊卵泡颗粒细胞增殖机制。利用不同浓度FSH处理绵羊颗粒细胞(GCs),采用CCK-8法检测细胞活性,流式细胞仪测定细胞凋亡和细胞周期变化,Western-Blot检测p-AKT和p-FOXO1蛋白质表达,qPCR分析PCNA、CCnd-2和Bcl-2基因表达量变化。结果表明:10ng/mL的FSH能显著提高GCs活性,抑制细胞凋亡(P0.05);AKT/FOXO信号通路的抑制剂AT7867预处理GCs后明显降低其活性(P0.05);在FSH的作用下,GCs中AKT蛋白的磷酸化水平显著升高,而FOXO1的磷酸化水平显著降低(P0.05),同时显著上调了PCNA、CCnd-2和Bcl-2的表达水平(P0.05)。本研究表明FSH通过AKT/FOXO1信号途径促进了绵羊GCs的生长、增殖。  相似文献   

Vergleichende Untersuchungen zur Steuerung der Ovarfunktion beim Rind mit PMSG und FSH     

G. Blödow  E. Kanitz  M. Kitzig  W. Kanitz  U. Duschinski 《Reproduction in domestic animals》1993,28(2):97-107

Inhalt: Nach Steuerung der Ovarfunktion beim Rind mit PMSG/PGF_2α und FSH/PGF_2α war 6.5 h nach der PGF_2α-Applikation eine deutliche Abhängigkeit zwischen den Estradiol-17β (E₂)- bzw. Testosteron (T)-Konzentrationen in der Follikelflüssigkeit und der Gröβe der Ovarialfollikel zu erkennen. In präovulatorischen Follikeln wurden unabhängig von der gewählten Hormonbehandlung teilweise signifikant höhere (P < 0,05) mittlere E₂- und LH-Konzentrationen als in nonovulatorischen Follikeln festgestellt. Follikel mit intakten oder degenerierten Oozyten wiesen keine signifikanten Unterschiede im Steroidgehalt auf. Der Reifungsprozeß der Cumulus-Oozyten-Komplexe bzw. Oozyten war mit charakteristischen Verschiebungen in den intrafollikulären Hormonspiegeln verbunden. Auch die veränderten Verhältnisse der Steroidhormonkonzentrationen in der Follikelflüssigkeit bestätigen die Rolle der Steroide bei der Oozytenreifung.  相似文献   

猪卵泡抑制素主动免疫对山羊生殖的影响   总被引：4，自引：0，他引：4  

刘桂琼  姜勋平  丁家桐  杨利国 《畜牧兽医学报》2003,34(1):37-39

抑制素是由动物的性腺分泌的一种糖蛋白,主要生理作用是反馈性抑制ＦＳＨ的合成和分泌。用抑制素对动物进行主动免疫,产生抑制素抗体中和内源性抑制素,从而削弱或解除它对ＦＳＨ分泌的抑制作用,引起动物排卵数增加,可能达到多产的目的。这种方法不仅与在生产和实验中广泛应用的促性腺激素处理一样可以提高动物的排卵率,而且可以弥补促性腺激素处理个体反应不一的缺陷,同时还能提高处理后几个胎次的产仔数,这在畜牧生产中有重要的应用价值。为此,本实验制备猪卵泡抑制素,并用它免疫山羊,探讨它用于提高家畜繁殖力的方法和可能性。1　材料…  相似文献   

A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit     

María Pilar Viudes‐de‐Castro  Francisco Marco‐Jimnez  Alba Ms Pellicer  Ximo García‐Domínguez  Amparo M. Talavn  Jose Salvador Vicente 《Reproduction in domestic animals》2019,54(4):696-701

Superovulation protocols are designed to achieve maximum embryo yields. Nevertheless, ovarian response control and the quality of obtained embryos are still a challenge. On the other hand, to save the superovulated embryos until their subsequent use, it is usual to cryopreserve them, so it is also crucial to assess their cryotolerance. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (FSH‐CTP) alone or supplemented with human chorionic gonadotropin (hCG) and to determine the impact of this stimulation on in vitro and in vivo development of fresh or devitrified embryos. Our outcomes showed that ovulation rate and recovered embryos were significantly increased when hCG was used. In vitro development of fresh and devitrified embryos and survival at birth were not significantly affected by superstimulation treatment. Results of this study suggest that a single injection of long‐acting FSH‐CTP supplemented with hCG can be effectively used in rabbits to elicit an increase in ovulation rate and number of recovered embryos. Furthermore, we demonstrated that hCG supplementation had no negative effects in embryo cryosurvival and development, showing similar survival rate at birth than FSH‐CTP alone group.  相似文献   

猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析     

范一萍  王晓梅  贾启涛  李成勃  梁小娟  曹春伟  周磊  陶聪  赵建国  刘剑锋  王彦芳 《中国畜牧兽医》2019,(3):774-781

试验旨在研究催乳素受体(prolactin receptor,PRLR)和卵泡刺激素β(follicle-stimulating hormoneβ,FSHβ)基因的多态性及其与母猪繁殖性状的关联分析。采用PCR-RFLP方法对大白猪、杜洛克猪和长白猪3个猪种共487头母猪进行了PRLR和FSHβ基因多态性检验,并采用单因子方差分析、LSD法分析不同基因型与母猪总产仔数、产活仔数、断奶窝重和断奶窝仔数等繁殖性状的相关性。结果表明,PRLR基因在大白猪和长白猪中B等位基因均为优势基因,基因频率分别为0.717和0.548,大白猪BB基因型总产仔数显著高于AA基因型(P<0.05),长白猪BB基因型断奶窝重显著高于AB基因型(P<0.05);FSHβ基因在大白猪、杜洛克猪和长白猪3个猪种中B等位基因均为优势基因,基因频率分别为0.804、0.760和0.789,长白猪BB基因型总产仔数和产活仔数均显著高于AB基因型(P<0.05),呈现BB>AA>AB趋势。因此,PRLR和FSHβ基因对荣昌猪场母猪繁殖性能有一定影响,可作为母猪繁殖性能分子选育的候选参考基因。  相似文献