砂仁及其混伪品益智仁的ISSR分子鉴别     

王梦圆  王霞  张典  王悦 《分子植物育种》2021,19(2):562-567

为创建砂仁及其主要混伪品益智仁的ISSR分子鉴别方法,以UBC818为引物,对影响ISSR-PCR反应体系的引物、dNTPs、DNA模板、Taq DNA聚合酶和Mg2+浓度进行5因素4水平正交优化试验,并在此基础上筛选阳春砂ISSR引物及砂仁正伪品的鉴别引物.结果表明,20 μL阳春砂ISSR-PCR最佳反应体系包括引...  相似文献   

七味养心健脾胶囊中五味子提取工艺研究     

卫强  杜会兰  戴一  杨春燕 《中兽医医药杂志》2012,31(1):47-49

目的:研究七味养心健脾胶囊中五味子的提取工艺条件。方法:以木脂素类化合物含量为考察指标,考察了五味子醇提、水提和渗漉的提取效果,用正交试验考察五味子的醇提工艺。结果:用醇提法提取五味子中总木脂素得率最高,故选用此法进行提取较佳;对五味子醇提工艺进一步优化,优化条件为:加入5倍70%乙醇,提取3次(2、2、1 h)。结论:该工艺稳定性良好,为改良七味养心健脾胶囊中五味子提取工艺奠定了研究基础。  相似文献   

枸杞红色素的稳定性及其在食品中的应用研究     

李金梅  杨秋林  赵智宏 《内蒙古农业大学学报(自然科学版)》2012,33(1):243-247

本文对枸杞红色素的稳定性进行了初步研究,研究发现枸杞红色素40℃以下比较稳定;还原剂(Na2SO3)对枸杞红色素稳定性影响不大,光照、高温、氧化剂(双氧水)能大大降低构杞红色素的稳定性;枸杞红色素在酸性、中性、弱碱性的环境中比较稳定,在强碱性环境中稳定性较差;Fe3+、Cu2+、Fe2+等金属离子对枸杞红色素稳定性影响较大,Ca2+、Na+等金属离子对枸杞红色素稳定性影响不大;枸杞红色素在酸奶和果汁中应用情况较好.  相似文献   

3种枸杞的HPLC-DAD图谱比较   总被引：8，自引：1，他引：8  

聂国朝 《福建林学院学报》2004,24(2):162-164

以甜菜碱和类胡萝卜素为主要活性成分的枸杞提取物为基础,使用液相色谱与二极管列阵检测器联用仪(HPLC DAD),给出了3种枸杞的HPLC DAD的化学指纹图谱,并进行了比较 说明可以用中药材化学指纹图谱来进行枸杞品种的识别和真伪的鉴定  相似文献   

枸杞多糖组分3对小鼠抗脂质过氧化作用的影响   总被引：6，自引：0，他引：6  

王建华  王汉中  张民  张声华 《中国兽医学报》2002,22(3):267-268

用枸杞多糖组分 3(L BP3)灌喂小鼠 31d后 ,测定小鼠体内超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶(GSH - Px)的活性 ,以及丙二醛 (MDA)和脂褐素的含量。结果表明 ,与对照相比 ,L BP3可提高或显著提高 (P<0 .0 1)小鼠体内 SOD和 GSH- Px的活性 ,并能降低或显著降低 (P<0 .0 1)体内 MDA和脂褐素的含量。由此表明 ,L BP3在小鼠体内具有抗脂质过氧化作用  相似文献   

Effect of intermittent hypoxia on bladder detrusor cell apoptosis and regulatory mechanism of Alpiniae oxyphyllae Fructus     

SU Miao-shang  XUE Si-chen  XU Li  REN Xi-kai  HUANG Nan  TANG Zhu-qin  XU Man-huan 《园艺学报》2021,36(12):2212-2219

AIM To investigate the effect of intermittent hypoxia （IH） on bladder detrusor cells apoptosis and calcium channel, and to discuss the regulatory mechanism of Alpiniae oxyphyllae Fructus （AOF）. METHODS IH model of bladder detrusor cells was established by treating the cells with 6 cycles of 5% O₂ for 60 min and 20% O₂ for 30 min. Human bladder detrusor cells were cultured in vitro, randomly divided into 6 groups, each group had 8 holes. P₂X₃ receptor antagonist + IH （A） group, M₃ receptor antagonist + IH （B） group, β₃ receptor antagonist + IH （C） group, AOF + IH （D） group, saline + IH control （NC） group and air simulation control （AC） group were set up. The cells density and morphology were identified by the methods of counting chamber and immunofluorescence light microscopy （LM） after interventions. The apoptosis was analyzed by flow cytometry. Calcium channel expression was detected by patch clamp. RESULTS （1） Compared with the cells in AC group, the cells density and activity were significantly increased in NC group （P<0.05）; some cells appeared protrusions, turned round and blur in cell borders. （2） The results of immunofluorescence for detecting α-SMA protein expression showed that, compared with the cells in group AC, the mean absorbance （MA） in group NC was significantly increased （F=3.25, P<0.05）; compared with the cells in group NC, that in group A and group D was both decreased significantly （P<0.05）. （3） Compared with the cells in group AC, the apoptotic rate was significantly decreased in group NC （P<0.05）; Compared with the cells in group NC, the apoptotic rates in group A and group D were both significantly increased （P>0.05）. （4） Compared with the cells in group AC, calcium ion channel expression was significantly decreased （P<0.05）. Compared with the cells in group NC, calcium ion channel expression in AOF （100 mg/L） and AOF （50 mg/L） group was significantly increased （P<0.05）. CONCLUSION IH regulates bladder detrusor cells proliferation and apoptosis through P₂X₃ bladder nerve receptors, high or moderate dose of AOF may change calcium channel and play a protective role in IH induced cell damage.  相似文献   

连吴茱萸质量标准研究     

石典花  张军  孙立立 《勤云标准版测试》2015,(4)

目的：建立连吴茱萸质量标准。方法：对连吴茱萸中主要有效成分吴茱萸碱、吴茱萸次碱和柠檬苦素进行测定,另外对连吴茱萸的性状、鉴别、水分、总灰分和醇浸出物也进行了研究。结果：连吴茱萸中水分含量为5.96%～10.91%,总灰分含量为6.51%～9.31%,醇浸出物含量为47.29%～50.69%,吴茱萸碱和吴茱萸次碱总量在0.064%～1.080%之间,柠檬苦素含量在1.451%～2.931%之间。结论：建立的质量标准可用于规范连吴茱萸的质量,保证临床疗效。  相似文献   

菌痢消颗粒薄层色谱研究     

卢亚艺 《中国兽药杂志》2009,43(6):35-37

菌痢消颗粒是由黄连、诃子、苦参、白头翁、甘草等中药制成的纯中药制剂,具有清热燥湿,泻火解毒,涩肠止泻之功效。在对本品的质量标准研究中,本文重点进行了薄层色谱研究,选择了多种展开系统进行比较。结果显示,采用《中国兽药典》收载方法及展开系统对本方中黄连、诃子、苦参进行薄层色谱鉴别,斑点清晰,方法可行。  相似文献   

Role of ROS/PKC/p38 MAPK pathway in protective effects of polysaccharide from Fructus cornion rat cardiomyocytes against hypoxia-reoxygenation injury     

LAI Li-na  SONG Li-hua  ZHANG Xiao-jing  ZHANG Xiao-yi  LEI Jing-wen  LIU Fang  GUO Chun-hua  SONG Xiao-liang 《园艺学报》2014,30(12):2201-2205

AIM: To investigate the effect of polysaccharide from Fructus corni(PFC) on cardiomyocytes against hypoxia/reoxygenation (H/R) injury and its possible relationship with ROS/PKC/p38 MAPK pathway.METHODS: Primary cardiomyocytes were isolated from neonatal SD rats and randomly divided into normal group, H/R group, PFC (20 mg/L, 100 mg/L and 200 mg/L) preconditioning+H/R groups, chelerythrine+PFC (100 mg/L)+H/R group and SB203580+PFC (100 mg/L)+H/R group. The cell viability was measured by inverted microscopic observation. Apoptosis in the cardiomyocytes was detected by Hoechst 33258 staining and fluorescence microscopy. The levels of lactate dehydrogenase (LDH) and superoxide dismutase (SOD) in the cell culture supernatants, and the reactive oxygen species (ROS) in the cells were also measured by microplate reader. The protein levels of PKC, p-p38 MAPK and HSP70 in the cells were detected by Western blotting.RESULTS: Compared with normal group, the cell viability and beating frequency were decreased in H/R group. LDH and ROS contents, apoptotic rate and p-p38 MAPK level increased significantly (P<0.01). Compared with H/R group, PFC preconditioning increased beating frequency, SOD activity and the protein level of PKC and HSP70, and decreased ROS production, the protein level of p-p38 MAPK and cell apoptotic rate. However, the effect of PFC was inhibited by chelerythrine or SB203580.CONCLUSION: PFC may protect cardiomyocytes from hypoxia/reoxygenation injury. Its mechanism is possibly involved in the inhibition of ROS via increasing the activity of SOD and the activation of PKC, and suppression of excessive activation of p38 MAPK.  相似文献   

烯效唑对罗汉果试管苗生长的影响   总被引：6，自引：0，他引：6  

周祖富  艾素云  杨美纯  黎兆安 《福建果树》2005,(1):13-15

将罗汉果试管苗接种于添加不同浓度烯效唑(S-3307)的改良MS培养基中，结果表明：合适浓度(0．01～0．1mg／L)的S-3307能有效延缓罗汉果试管苗的伸长，使苗矮化增粗；同时促进不定根发生，根系发达，苗健壮。但高浓度的S-3307使苗的生长受到过度抑制，植株过于矮化，表现不正常。  相似文献