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金定鸭谷草转氨酶同工酶的研究 总被引：2，自引：0，他引：2
本文研究了金定鸭谷草转氨酶同工酶，结果表明，在聚丙烯酰胺凝胶电泳中可将其区分成两种形式的同工酶（Ｓ－ＧＯＴ，Ｍ－ＧＯＴ）。该同工酶在薄层等电聚焦电泳中，Ｍ－ＣＯＴ可再区分为两条电泳带，Ｓ－ＧＯＴ则被区分为８条区带。它们的等电点分别在ＰＨ８．４～７．９和７．４～５．９之间。同工酶在各组织器官中的分布有明显的不同，其总活力由大到小的顺序分别为脏＞肝＞脑＞胸肌＞腿肌＞肾。 相似文献
22.
家蚕后部丝腺转氨酶活性及其与茧质性状关系 总被引：1，自引：0，他引：1
采用酶学速率法分析了８个家蚕品种末龄第３、５、７日后部丝腺谷丙转氨酶（ＧＰＴ）、谷草转氨酶（ＧＯＴ）活力和比活力。少丝量品种末龄ＧＰＴ、ＧＯＴ比活力随末龄发育逐渐升高，多丝量品种ＧＰＴ比活力随末龄发育而不断下降，而ＧＯＴ比活力一直较高或略有升高。ＧＰＴ、ＧＯＴ比活力与茧层率、茧层量、全茧量的遗传相关，末龄第３天为显著正相关，第５天相关不显著，第７天为极显著负相关。ＧＯＴ、ＧＰＴ比活力遗传力分别达８７．４４％、８０．４％，可作为产丝量选择的重要生化指标。 相似文献
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为了了解油污净的残留是否对环境有一定的影响,能否对生物体造成一定的毒害作用。笔者以泥鳅为受试对象,研究油污净在不同体积分数和不同处理时间内对泥鳅遗传毒性及生理毒性的影响,旨在探讨油污净的安全性,从而为合理使用油污净提供参考。试验结果表明,①随着处理组油污净体积分数由0.14、0.15、0.16至0.17 mL/L的升高和处理时间由3、6至9 d的延长,红细胞的微核率同对照组相比呈增高趋势。②在生理毒性对照组中,处理组泥鳅血清中的谷草转氨酶(GOT)和谷丙转氨酶(GPT)活性随着处理时间的延长而有增加趋势,但与处理组体积分数线性关系呈先升高后下降的趋势,表明油污净能够影响血清中GOT和GPT的活性。 相似文献
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Summary Employing polyacrylamide gradient-gel electrophoresis, the genetic control of leaf glutamate oxaloacetate transaminase (GOT) isozyme variants and their linkage to ds1 (desynapsis), y (tuber flesh colour), cr (crumpled) and yc (yellow cotyledon) were studied in diploid tuberous Solanum species hybrids.Leaf GOT isozymes proved to be dimeric and under the control of two independently segregating loci (Got-1 and Got-2) with two (1
s
, 1
f
) and three (2
s
, 2
f
, 2
n
)alleles respectively. The 2
n
allele was concluded to encode a 2
fallozyme subunit capable of forming heterodimeric but no homodimeric active molecules. As expected with Got-1 and Got-2 isozymes being localized in different cellular compartments, no intergenic heterodimer formation was observed.In contrast to earlier studies, a gradual rather than discrete transition between the tuber flesh colour classes white and yellow was observed in segregating progenies. Adopting a standardized classification method, however, monogenic recessive inheritance of white tuber flesh was confirmed.
Got-1, ds1 and cr were found to represent a single linkage group. Among the presently studied marker loci no further linkages were detected. As to linkage analysis, the difficulties imposed by gametophytic incompatibility in nearly all diploid potato species and by the frequent occurrence of recessive (sub)lethal genes are discussed. 相似文献
25.
氮磷钾施用对附子几种氮代谢酶活性的影响 总被引：2，自引：0，他引：2
运用三因素二次回归正交组合设计,试验研究了氮、磷、钾三因素对中药附子(乌头)的过氧化物酶(POD)、硝酸还原酶(NR),谷草转氨酶(ALT/GOT)和谷丙转氨酶(AST/GPT)活性的影响效应。结果表明:N、P施用量对附子NR、GOT、GPT均有显著的影响,除较大施用量条件下对GOT活性的施P效应>施N效应外,其余情况下对三种酶均为N效应>施K效应;施K除对NR活性有明显效应外,对GOT和GPT没有明显影响;N、P、K施用对POD活性无明显效应。单施N对GOT、NR和单施P对NR均为正效应,随施用量增加酶活性升高;单施P对GOT、GPT和单施N对GPT在施用量低于零水平时,随施用量的增加酶活性升高,超过零水平后随施用量的增加酶活性下降;单施K时,对NR活性则是在零水平时最低,低钾和高钾时NR活性均较高。对几种酶的效应中,仅对GOT存在施N与施P之间的互作效应,随着N施用量增加施P对GOT活性的增加效应更明显。 相似文献
26.
GOT、GPT、LDH、钙、磷与鸡产蛋性能的关系 总被引：1，自引：0，他引：1
我们测定了伊沙 B300父系和母系母鸡血清中 GOT、GPT、LDH 的活性和钙瞬含量,并探讨了300日龄蛋鸡生产性能的有关指标,旨在为育种工作提供科学依据。试验结果,GOT、GPT、LDH 活性和钙瞬含量与蛋鸡生产性能相关程度不同。其中 GPT 对蛋鸡生产性能相关极显著(P<0.01),GOT 和 LDH 与蛋鸡生产性能相关不显著(P>0.05)。为此我们进一步进行了 GPT 活力与主要产蛋性能的比较试验。 相似文献
27.
Aspartate amino transferase (AspT; EC: 2.6.1.1) is one of the key enzyme of aspartate malate shuttle and non-essential amino acid metabolism. In order to clarify the change of the activities of both cytosolic (c-) and mitochondrial (m-) AspT isoenzymes during the different reproductive stages of Clarias batrachus, an air-breathing carnivorous freshwater fish, the c- and m- AspT activities were measured spectrophotometrically in liver, heart, kidney, brain and ovary during the pre-spawning, spawning and post-spawning phases of the annual reproductive cycle of this fish. Maximum activities were observed during the spawning phase, followed by pre- and post-spawning phases. The native agarose gel electrophoretic pattern revealed two AspT forms, c-AspT and m-AspT, throughout the pre- to post-spawning phases. However, the spawning phase was marked by the expression of an additional anionic form of c-AspT in all tissues studied. These results indicate a distinct utilization of aspartate particularly in the spawning phase in fish in the central metabolic path via transamination reactions, and suggest a control of the enzyme activity by the expression of the additional c-AspT gene, especially at the spawning phase. 相似文献
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不同体积水浴平衡法和8层纱布包被平衡法对绵羊冷冻精液品质影响比较研究的结果表明:250 mL和200 mL 38 ℃ 体积水浴平衡的冷冻精子的活率、生存指数、顶体完整率分别为0.43和0.45、3.57和3.55,47.60 %和47.98 %,显著高于150 mL、100 mL、50 mL 38 ℃体积水浴和8层纱布包被平衡冷冻精子的活率、生存指数、顶体完整率(P<0.05);250 mL和200 mL38 ℃ 体积水浴平衡的冷冻精子GOT释放量分别为303.33和303.86(UL-1)(P<0.05)。200 mL38 ℃ 体积水浴平衡到达4℃的时间比250 mL38 ℃ 水浴平衡缩短0.5 h,因此,推荐生产实践中应用200 mL 38 ℃ 体积水浴2.5 h降温到4℃的平衡方法。 相似文献