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11.
为探明除草剂乙氧氟草醚(Oxyfluorfen)对水生生物的毒性,以大鳞副泥鳅(Paramisgurnus dabryanus)为受试对象,进行乙氧氟草醚对大鳞副泥鳅的急性毒性、生理毒性和DNA损伤试验。结果表明:随着染毒浓度增加和时间的延长,大鳞副泥鳅的死亡率升高,安全浓度为8.40mg/L,根据中国《化学农药环境安全评价试验准则》,乙氧氟草醚对大鳞副泥鳅为低毒。乙氧氟草醚低浓度(8.00 mg/L,10.50mg/L,13.00mg/L)作用下,大鳞副泥鳅肝脏谷氨酸草酰乙酸转氨酶(GOT)、谷氨酸丙酮酸转氨酶(GPT)活性明显升高;高浓度(15.50mg/L)作用下酶活性在染毒2d、4d时呈上升趋势,但在6d时急速下降。15.50mg/L组与空白对照组相比,大鳞副泥鳅肝细胞的彗尾DNA百分含量、彗星尾长和Olive尾矩明显增加,肝细胞受到明显损伤。 相似文献
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GPA对小鼠肝损伤血清及肝组织中GOT、GPT的影响 总被引：1，自引：0，他引：1
采用刀豆蛋白A（ConA）尾静脉注射方法建立小鼠免疫性肝损伤模型,分别用低（100mg/kg）、中（200mg/kg）、高（400mg/kg）3个不同剂量大蒜多糖（GPA）对小鼠进行灌胃,灌服7d后,小鼠尾静脉注射刀豆蛋白A,采血及取肝组织,用赖氏法测定天冬氨酸转氨酶（GOT）及丙氨酸转氨酶（GPT）变化。结果模型组和多糖组与空白组比较,除高浓度多糖组差异不显著（P〉0.05）外,其它各组均差异显著（P〈0.05）,且多糖组与模型组比较差异显著（P〈0.05）,并呈现一定的量效关系。表明不同浓度的大蒜多糖均对ConA致小鼠免疫性肝损伤具有保护作用。 相似文献
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饲料锌水平对肉用仔鸡血清中酶活性的影响 总被引：5，自引：0，他引：5
选择１０日龄Ａｒｂｏｒ Ａｃｒｅｓ雏鸡３００只，随机分成６组，每组５０只。第一组饲以含锌３０μｇ·ｇ的玉米－豆饼型基础日粮，其它五组日粮依次含锌６０μｇ·ｇ，１１０μｇ·ｇ，１６０μｇ·ｇ，２１０μｇ·ｇ，２６０μｇ·ｇ。５０日龄时，分别进行心脏采血，测血清中淀粉酶、乳酸脱氢酶（ＬＤＨ），谷丙转氨酶（ＧＰＴ），碱性磷酸酶（ＡＫＰ）的活性及血清含锌量。结果表明，在试验条件下，１，６组血清淀粉酶的活怀 相似文献
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对本校965名职工进行了 HB_sAg 及肝功能的检测。结果查出 HB_sAg 阳性50例,阳性率5.18%。在 HB_sAg 阳性者中:肝功能异常者27例,占54.5％;以20岁～29岁阳性率最高;性别和职业上无明显差异。19例乙型肝炎病人的配偶 HB_sAg 均为阴性。915例 HB_sAg 阴性者中肝功能异常者28例,占3.1％,单项转氨酶高的54例,占5.9%。 相似文献
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饲料锌水平对肉用仔鸡肝脏中含锌酶活性的影响 总被引：7，自引：0，他引：7
选择１０日ＡＡ肉用仔鸡３００只，随机分成６组，每组５０只。第一组为对照组，饲以含锌３０ｍｇ／ｋｇ的玉米～豆饼基础日粮，其它五组依次含锌６０、１１０１６０、２１０和２６０ｍｇ／ｋｇ。至５０日龄时，测肝脏中超氧化物歧化酶（ＳＯＤ）、乳酸脱氢酶（ＬＤＨ）、谷丙转氨酶（ＧＰＴ）、碱性磷酸酶（ＡＫＰ）的活性和肝组织中的含锌量。结果表明，在试验条件下，ＳＯＤ活性随饲料锌水平的提高基本不变，ＬＤＨ、ＡＫＰ、ＧＰＴ活性及锌含量随饲料锌水平的提高而提高。 相似文献
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The radioprotective property of an ethanolic extract of Piper longum fruits (EEPLF) was investigated in Swiss mice. The white blood cell (WBC) count in irradiated control mice was drastically reduced to 1900 cells/mm3 on third day but in treated animals the count was 2783.3 cells/mm3. The number of bone marrow cells and alpha-esterase positive cells was also enhanced by the EEPLF administration (16.7 x 10(6) cells/femur and 946.5/4000 cells, respectively) when compared to the radiation exposed control animals (12.2 x 10(6) cells/femur and 693.5/4000 cells, respectively). EEPLF reduced the elevated levels of glutathione pyruvate transaminase (GPT), alkaline phosphatase (ALP), and lipid peroxidation (LPO) in liver and serum of radiation treated animals. The extract administration also increased the reduced glutathione (GSH) production to offer the radioprotection. 相似文献
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Kiwifruit were stored in 0.25% O2 (ULO), 1% O2 (LO) and 2% O2 + 5% CO2 (CA) and the controls were kept in air. The fruit were held at 0 °C for 34 and 94 d of storage and, after these times, were transferred to 20 °C in air for 14 d of shelf life. During the short term storage (34 d), a significant increase in anaerobic metabolites, above all ethanol, was observed in ULO, LO, and CA fruit (166, 131, 120 μL/L). After the shift to shelf life, a large and unexpected increase in PDC (pyruvate decarboxylase), ADH (alcohol dehydrogenase in the direction of ethanol oxidation), LDH (lactate dehydrogenase), and GPT (glutamate-pyruvate transaminase) was observed, resulting in ethanol depletion (ULO) or no further increase, and an increase in acetaldehyde which, in turn, could have hastened fruit ripening. Even the control fruit showed an increase in ethanol during storage and an increase in enzyme activity during shelf life, especially in ADH, but to a lesser extent without an increase in acetaldehyde. During the long term storage, anaerobic metabolites (ethanol and acetaldehyde) still increased and GPT activity rose significantly in the ULO and CA samples. A burst of enzyme activity was also observed during the second shelf life in the CA and LO samples, but not in the control, while in ULO fruit the activity rose continuously. GPT activity showed the highest peaks in CA and ULO fruit. An ethylene burst was observed in ULO and CA fruit during the second shelf life (about 25 μL/kg-h) but not during the first shelf life. The potential role of these enzymes in kiwifruit stress response during storage and shelf life is discussed. 相似文献
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对4月龄的五龙鹅快长系饲喂添加不同比例冬牧-70鲜草的日粮,测定血浆中碱性磷酸酶(AKP)、谷丙转氨酶(GPT)和乳酸脱氢酶(LDH)的活性并进行相关性比较.试验结果表明,随着冬牧-70鲜草比例的增加,五龙鹅血浆中的AKP活性显著性降低(P<0.05),AKP活性与粗纤维(CF)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)消化率呈显著负相关(P<0.05),与Ca、蛋氨酸(Met)、胱氨酸(Cys)消化率呈显著正相关(P<0.05);GPT和LDH活性与CF,NDF和ADF消化率呈弱正相关(P>0.05),与Ca,Met,Cys消化率呈弱负相关(P>0.05). 相似文献
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5种中草药对鲤血清谷丙转氨酶、谷草转氨酶及红细胞过氧化氢酶活性的影响 总被引：1，自引：0，他引：1
用分别添加3%刺五加、蒲公英、枸杞子、金银花和大蒜单方中草药的基础日粮饲喂鲤(Cyprinus carpio),在第15 d和第30 d测定其血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)及红细胞过氧化氢酶(CAT)活性。结果显示,15 d时,血清GOT活性仅大蒜组与对照组差异显著,血清GPT及红细胞CAT活性各试验组与对照组差异均不显著;30 d时,血清GOT活性各实验组与对照组的差异均不显著,血清GPT活性刺五加组和蒲公英组与对照组差异显著,红细胞CAT活性各实验组与对照组的差异均不显著。 相似文献