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31.
GOT、GPT、LDH、钙、磷与鸡产蛋性能的关系 总被引：1，自引：0，他引：1
我们测定了伊沙 B300父系和母系母鸡血清中 GOT、GPT、LDH 的活性和钙瞬含量,并探讨了300日龄蛋鸡生产性能的有关指标,旨在为育种工作提供科学依据。试验结果,GOT、GPT、LDH 活性和钙瞬含量与蛋鸡生产性能相关程度不同。其中 GPT 对蛋鸡生产性能相关极显著(P<0.01),GOT 和 LDH 与蛋鸡生产性能相关不显著(P>0.05)。为此我们进一步进行了 GPT 活力与主要产蛋性能的比较试验。 相似文献
32.
J.?W.?Huang L.?X.?Tian Z.?Y.?Du H.?J.?Yang Y.?J.?LiuEmail author 《Fish physiology and biochemistry》2005,31(4):331-337
Pyridoxine is essential for animals. The purpose of this study was to investigate the pyridoxine deficiency symptoms and the
fit dose of pyridoxine to keep normal physiological function in grouper, Epinephelus coioides. Graded levels of pyridoxine (0, 1, 2, 4, 8, 16, 32 mg pyridoxine kg−1 diet) were fed to grouper juveniles (14.83±0.31 g) for 8 weeks, and then some physiological and biochemical parameters were
measured. Pyridoxine deficiency symptoms such as anorexia, poor survival, convulsion, helically swimming and hyperirritability
were observed in fish fed the pyridoxine-deficient diet, and these fish also showed low whole blood haemoglobin level, liver
glutamic-pyruvic transaminase (GPT) and superoxide dismutase (SOD) activities. Slight symptoms of pyridoxine deficiency also
appeared among fish fed the diet with very low dose (1 mg pyridoxine kg−1 diet) of pyridoxine in 6 weeks. Broken-line regression analysis showed that the optimal pyridoxine levels for these fish
to keep normal survival, whole blood haemoglobin level, liver GPT and SOD activities were approximately 1.75, 1.87, 2.22 and
2.05 mg kg−1, respectively. 相似文献
33.
Sung Hwoan Cho Sang-Mok Lee Byum Hee Park Sung-Choon Ji Jinhwan Lee Jeanhee Bae Sung-Yong Oh 《Fish physiology and biochemistry》2007,33(1):49-57
This study was performed to determine the effect of the dietary inclusion of various sources of green tea on growth, body
composition and blood chemistry of the olive flounder. Twenty-five juvenile fish were distributed into each of 15 180 l flow-through
tanks. Five experimental diets with triplicates were prepared: control, raw leaves, dry leaves, by-product and extract. The
5% various sources (raw leaves, dry leaves and by-product) of green tea were included in the experimental diets at the expense
of 5% wheat flour. The extract was diluted with water and mixed with the ingredients to maintain 5% green tea at the same
concentration as in the other diets. Weight gain and specific growth rate of fish fed the control diet and experimental diet
containing extract were higher than those of fish fed the diets containing raw and dry leaves and by-product. Feed and protein
efficiency ratio for fish fed the control diet and diet containing extract was higher than for fish fed the diets containing
raw leaves and by-product. The level of low-density lipoprotein (LDL) cholesterol of fish fed the control diet was higher
than that of fish fed the other diets containing various sources of green tea. The glutamic oxaloacetic transaminase (GPT)
concentration of fish fed the diets containing raw leaves and extract was lower than that of fish fed the control diet. Crude
lipid content of liver in fish fed the control diet was higher than that in fish fed the diets containing raw and dry leaves
and by-product but was not significantly different from that of fish fed the diet containing extract. Dietary inclusion of
extract was the most effective way to improve growth and feed utilization of the olive flounder among the various sources
of green tea, and all sources of green tea were effective in lowering serum LDL cholesterol and GPT of the fish. 相似文献
34.
【目的】探讨双酚A(bisphenol A,BPA)对泥鳅(Misgurnus anguillicaudatus)肝组织结构和谷丙转氨酶(glutamic-pyruvic transaminase,GPT)活性的影响,为评价酚类化合物对水生生物的毒害作用提供理论依据。【方法】根据预试验确定的BPA对泥鳅的安全质量浓度和最小不致死质量浓度,按等对数(常用对数)法将BPA质量浓度设置4个(1.9,2.3,2.7,3.0mg/L)处理,以未用BPA处理组为对照,分别在染毒7,14,21,28d取样,采用H.E染色法观察泥鳅肝组织结构的变化,采用赖氏比色法测定泥鳅肝脏GPT活性的变化。【结果】在1.9mg/L BPA中暴露28d时,泥鳅肝细胞肿大、空泡化及细胞溶解;在3.0mg/L BPA中仅暴露14d时,泥鳅肝细胞除严重肿大、空泡化及细胞溶解外,还出现核变形与核溶解等受损症状。与对照组相比,4个BPA质量浓度组泥鳅在暴露各个时期GPT活性均有明显升高,但随着BPA质量浓度的增大,GPT活性逐渐降低;在同一BPA质量浓度下,随着暴露时间的延长,GPT活性呈现先升高后降低的趋势。【结论】BPA可造成泥鳅肝组织结构损伤,并随BPA质量浓度的增大而加剧;BPA对泥鳅肝脏GPT的活性具有显著影响,可导致肝功能受损。 相似文献
35.
沙棘油口服液对运动训练小鼠肝脏组织自由基代谢和超微结构影响的实验研究 总被引：3，自引：0，他引：3
通过测定运动小鼠大脑超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,观察其超微结构变化,探讨服用沙棘油对小鼠能力影响的生物化学机制。为此选用70只雄性ICR小鼠随机分为六组:安静对照组、安静沙棘组、训练即刻组、训练即刻沙棘组、训练恢复24h组和训练恢复24h沙棘组。经过6周(42d)游泳耐力训练后进行一次力竭性游泳,分析比较了各组小鼠血清谷草转氨酶(GFT)、肝脏超氧化物歧化酶(SOD))活性、丙二醛(MDA)含量等指标,同时还对各组肝脏细胞超微结构进行了电镜观察。结果:1)以上各组GPT除安静对照组与相应沙棘组和训练即刻组与训练恢复组间无显著差异外(P>0.05),其余各组间均有显著差异(P<0.05);2)SOD除安静对照组与相应沙棘组,训练即刻沙棘组与训练恢复沙棘组间无显著差异外(P>0.05),其余各组间均有显著差异(P<0.05);3)MDA除训练即刻组与相应沙棘组及恢复组间无显著差异外(P>0.05),其余各组间均有显著差异(P<0.05);4)沙棘组肝脏细胞中线粒体、肝浆网、肌丝等结构均比相应对照组完整,病变程度比对照组轻。结果表明,沙棘油能改善运动对肝脏细胞的损伤,提高肝脏组织抗自由基氧化的能力,同时还对肝脏细胞超微结构具有明显保护作用。 相似文献
36.
对我国肖山鸡、白耳鸡、狼山鸡、乌骨鸡、油鸡、白耳鸡（）×肖山鸡（♀）、肖山鸡（）×白耳鸡（♀）等７个地方鸡种（含２个杂种）１２周龄血浆碱性磷酸酶（ＡＫＰ）、谷丙转氨酶（ＧＰＴ）、肌酸激酶（ＣＰＫ）的活性进行了比较研究。结果表明：狼山鸡、肖山鸡血浆ＡＫＰ活性显著高于白耳鸡和乌骨鸡（Ｐ＜０．０５）;公鸡血浆ＡＫＰ活性显著高于母鸡（Ｐ＜０．０５）;血浆ＡＫＰ活性与１２周龄体重呈极显著相关（Ｐ＜０．０１）;白耳鸡、乌骨鸡血浆ＧＰＴ活性显著高于狼山鸡（Ｐ＜０．０５）;公鸡血浆ＧＰＴ活性有高于母鸡的趋势,但检验结果不显著（Ｐ＞０．０５）。血浆ＧＰＴ活性与１２周龄体重相关分析表明,油鸡达显著水平（Ｐ＜０．０５）;乌骨鸡、肖山鸡、白耳鸡（）×肖山鸡（♀）血浆ＣＰＫ活性显著高于其他地方鸡种（Ｐ＜０．０５）,狼山鸡显著高于肖山鸡（）×白耳鸡（♀）（Ｐ＜０．０５）;母鸡血浆ＣＰＫ活性有高于公鸡的趋势,但差异不显著（Ｐ＜０．０５）;血浆ＣＰＫ活性与１２周龄体重的相关呈负相关趋势,但不显著（Ｐ＜０．０５） 相似文献
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38.
试验选用80头平均体重为6．16±0．13kg的长白×约克夏仔猪,28±1 d断奶后,按体重相近原则随机分为 5个处理,研究了不同水平的谷氨酰胺(Gln)对断奶仔猪血清中谷丙转氨酶(GPT)、碱性磷酸酶(AKP)活性的影响。 Gln的添加量分别为0％、03％、0．6％、0．9％和1．2％。试验期为21 d。于试验的第7、14和21天,分别测定血清中 GPT、AKP活性。结果表明,日粮中添加Gln能够显著提高断奶仔猪断奶后7、14、21 d血清中谷丙转氨酶活性(P≤ 0．05)。第7、14天时,在日粮中添加Gln,对碱性磷酸酶的活性影响显著(P≤0．05)。谷氨酰胺在断奶仔猪日粮中最适宜添加量为1．2％。 相似文献
39.
1987～1988年分别在大田和盆栽条件下,在水稻抽穗前22天,用生长延缓剂多效唑(MET)处理,结果表明:每亩17g 多效唑,降低了剑叶叶绿素含量,提高了顶二叶、顶三叶的叶绿素含量和光合强度;叶片可溶性蛋白质含量降低,但延缓可溶性蛋白下降速度;抽穗后6天,剑叶 GPT 活性增高,GOT 活性降低;抽穗后8天,和13天,处理的籽粒 GPT 活性增加。此外,还加强了籽粒发育后期的灌浆速率,提高了结实率和千粒重,籽粒产量增加12.8%,同时还提高了籽粒蛋白质、氨基酸和 VB_1的含量。本文就多效唑对水稻籽粒品质和产量效应进行了综合评价,并讨论了开发多效唑潜力的途径。 相似文献
40.
镉对罗氏沼虾组织转氨酶活性及组织结构的影响 总被引：33，自引：2，他引：33
对罗氏沼虾（Ｍａｃｒｏｂｒａｃｈｉｕｍｒｏｓｅｎｂｅｒｇｉｉ）成虾六种组织的谷丙转氨酶（ＧＰＴ）和谷草转氨酶（ＧＯＴ）活性正常值作了测定，其中心脏和绿腺的ＧＰＴ和ＧＯＴ活性较高，鳃和肝胰脏次之，卵巢和肌肉较低。不同镉离子浓度（０．３７、０．７４和１．４８×１０－６）对罗氏沼虾的急性中毒试验表明，受水中０．７４和１．４８×１０－６Ｃｄ２＋影响，心脏、鳃和肝胰脏的ＧＰＴ和ＧＯＴ活性显著降低；绿腺的ＧＰＴ和ＧＯＴ活性在０．７４×１０－６组显著升高。组织学观察显示，Ｃｄ２＋中毒引起鳃丝增粗，鳃丝血腔扩大；肝小管严重萎缩，肝细胞解体；绿腺细胞解体，迷路结构消失。 相似文献