Use of GnRH-agonists for Medical Management of Reproductive Disorders in Birds     

《Veterinary Clinics of North America: Exotic Animal Practice》2014,17(1):23-33

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哺乳动物下丘脑-垂体-卵巢轴的研究进展   总被引：3，自引：1，他引：3  

王凌燕  王树迎  侯衍猛  谭雷涛 《动物医学进展》2005,26(7):8-11

哺乳动物的下丘脑、垂体和卵巢分泌的激素在功能上相互作用,构成一个完整的神经内分泌生殖调节体系,即下丘脑垂体卵巢轴,它在生殖活动中起着主要的调节作用。下丘脑中分布的GnRH神经元可以分泌GnRH,GnRH调节垂体中促性腺激素细胞分泌促性腺激素FSH和LH,促性腺激素作用于卵巢受体,引起雌激素和孕酮分泌并影响生殖活动。从组织学角度上研究,下丘脑垂体卵巢轴中的结构,如GnRH神经元、促性腺激素细胞、卵泡随周期性变化而呈现出不同的形态结构和分泌特点。因此,对以上各种细胞的研究是探讨其所分泌激素的基础,而下丘脑垂体卵巢轴中的各种激素的研究则是了解和控制动物繁殖机能的关键。  相似文献   

Gonadotropin releasing hormone receptor gene and protein expression and immunohistochemical localization in bovine uterus and oviducts     

Singh R  Graves ML  Roskelley CD  Giritharan G  Rajamahendran R 《Domestic animal endocrinology》2008,34(3):319-326

Recently GnRH, GnRH-R systems has been demonstrated in various extrahypothalamic and extrapituitary reproductive tissues in different mammalian species, where GnRH acts in an autocrine and or paracrine manner and modulates different biological processes. GnRH-R mRNA has also been demonstrated in bovine ovaries (follicle and corpus luteum) and normal and carcinogenic human endometrium/endometrial cells. This is the first study elucidating presence of GnRH-R mRNA and GnRH-R protein in bovine uterus and oviducts in follicular and luteal phases of the estrous cycle and further localizing the receptors to endometrial and oviductal epithelial cells. To our knowledge this is the first report demonstrating GnRH-R mRNA and protein in mammalian oviducts. We used gene-specific primers and monoclonal GnRH-R antibody to test GnRH-R mRNA and GnRH-R protein through RT-PCR and immunobloting. Immunohistochemistry was employed to localize these receptors to endometrial and oviductal epithelial cells. GnRH-R mRNA and receptor protein were expressed at expected molecular weights of 920bp and 60kD, respectively. Densitometry analysis revealed that expression levels for GnRH-R protein in uterus and oviducts were similar to bovine pituitary. The presence of GnRH receptors in bovine uterus and oviducts is intriguing and it would be imperative to examine the functional role of this system in the regulation of reproductive processes.  相似文献   

Sequential preovulatory expression of a gonadotropin-releasing hormone-inducible gene,Nr4a3, and its suppressor Anxa5 in the pituitary gland of female rats     

Ryota TERASHIMA  Titaree LAOHARATCHATATHANIN  Shiro KURUSU  Mitsumori KAWAMINAMI 《The Journal of reproduction and development》2021,67(3):217

Functional relationship between nuclear receptor subfamily 4 group A member 3 (Nr4a3) and annexin A5 (Anxa5), which are two gonadotropin-releasing hormone (GnRH)-inducible genes, has been established while evaluating pituitary gonadotropes in relation to follicle-stimulating hormone beta (Fshb) expression. However, the physiological variations that arise due to the differential expression of these genes in the pituitary gland during rat estrous cycle remain unknown. This study aimed to evaluate the Nr4a3 and Anxa5 mRNA expression during the estrous cycle in rats in comparison with the expression of the gonadotropin subunit genes, luteinizing hormone beta (Lhb) and Fshb. Nr4a3 mRNA expression showed a single peak at 1400 h of proestrus during the 4-d estrous cycle. Anxa5 mRNA level was elevated along with increased Fshb mRNA expression after the decline of Nr4a3 mRNA until 2300 h. Lhb mRNA expression levels were not significantly changed during the estrous cycle. Notably, addition of a GnRH antagonist at 1100 h completely eradicated luteinizing hormone secretion at 1400 h and 1700 h of proestrus, and significantly reduced the Nr4a3 mRNA expression level at both the time points. These results suggest that GnRH is, at least partly, responsible for the increase in pituitary Nr4a3, and that the interaction between NR4A3 and ANXA5 is required to regulate Fshb expression during the preovulatory gonadotropin surge.  相似文献   

GnRH并列体二聚体主动免疫对公兔性腺的影响   总被引：1，自引：0，他引：1  

方富贵  章孝荣  张运海  袁运生  刘亚  陶勇 《中国兽医学报》2002,22(4):373-374

用GnRH并列体二聚体主动免疫公兔，观察了免疫对公兔性腺及体重的影响。结果表明，免疫不影响公兔体重的工，而使公兔睾丸重量降低，就纵长缩短，表明性腺有萎缩的趋势。  相似文献   

OvSynch法处理进行黄牛定时人工授精研究     

李军锋  张家骅  魏学良  徐恢仲  崔中庆  王志旺 《中国牛业科学》2002,28(2):13-16

利用国产GnRH、PGF2α和EB等外源激素对本地黄牛进行处理，观察了OvSynch处理进行定时人工授精的繁殖效果，并探讨了卵巢状况对GnRH作用是否存在影响。结果显示：（1）各试验组同期发情率与对照组差异不显著（P＞0．05），但发情同期化程度显著高于对照组，且能够集中定时进行人工授精；（2）试验Ⅰ、Ⅱ组的第一情期受胎率分别为60．0％、58．3％，与对照组（42．9％）之间无显著差异（P＞0．05）；（3）GnRH类似物能够调控卵泡发育波，其作用不受卵巢状况的影响。试验表明，采用国产GnRH对本地黄牛进行OvSynch处理，能够达到定时人工授精且不降低受胎率的目的。  相似文献   

奥利亚罗非鱼促性腺激素释放激素cDNA的克隆及原核表达     

曹丽萍  丁炜东 《上海海洋大学学报》2006,(3):270-275

促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)是下丘脑分泌产生的神经激素,对脊椎动物生殖的调控起重要作用。本研究通过RT-PCR方法从奥利亚罗非鱼丘脑中扩增出长约400 bp的目的序列GnRH,并将其克隆到T载体中,经酶切鉴定、序列测定分析后表明其与尼罗罗非鱼和乌颊海鲷具有较高的同源性,处于同一个进化分支上;而与日本青、金鱼、拟鲤、壁虎等的同源性较低。将此cDNA片段定向克隆到表达载体pMAL-c2x中构建重组表达质粒pMAL-GnRH,转化到大肠杆菌TB1中,0.3M IPTG诱导4 h后成功表达出与预期大小相符的约56 kD的融合蛋白。表明大肠杆菌表达系统可以成功地体外表达奥利亚罗非鱼GnRH,为进一步制备抗体了解其免疫调节作用奠定基础。  相似文献   

鱼体内性类固醇激素水平与催产药物相关性研究     

宋艳  郑虎  翁玲玲  曹阳  马世荣  林珏 《西南农业学报》2007,20(6):1369-1372

研究怀卵大鳞副泥鳅(Paramisgurnus dabryanus)用药后亲鱼血清、卵液性激素含量变化,探讨了药物组合和变化间的相关性。结果表明,GnRH-A及其配伍注射后,泥鳅血清中雌雄激素、卵液中孕激素含量在排卵前增加,呈明显的组合—效应关系;表明催产药物可引起血清、卵液性激素含量变化,并且这一变化有一定的规律性,可能会成为繁殖药物筛选的重要指标。  相似文献   

Practical protocols for timed artificial insemination of jennies using cooled or frozen donkey semen     

Feng Yang  Nan Li  Bing Liu  Jie Yu  Shuaishuai Wu  Ruitao Zhang  Weigang Yang  Chuanliang Ji  Qianqian Sun  Jinliang Ma  Min Li  Jingqian Zhou  Xiangshan Zhou  Melina Pietrani  Luis Losinno  Shenming Zeng 《Equine veterinary journal》2021,53(6):1218-1226

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Expression of reproductive-related genes and changes in oocyte maturation of goldfish broodstock (Carassius auratus) following injection of different exogenous kisspeptins     

AbdolMajid Valipour  Behrooz Heidari  Hamidreza Vaziri  S. Mohsen Asghari 《Reproduction in domestic animals》2021,56(10):1349-1357

Kisspeptin, upstream of the hypothalamic-pituitary-gonadal axis, play an essential role in the reproductive process. In the present study, the effect of different types of kisspeptin, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss) and their combination (Kiss1+H) on the reproductive-related genes (kiss1, Kissr and Cyp19) of adult female goldfish was investigated in comparison with Ovaprim (a synthetic GnRH hormone). Kiss1 and Hkiss were synthesized using a solid-phase synthesis approach. Peptides were injected at a dose of 100 μg/kg body weight. The brain and ovarian tissues of samples were separated for histological studies 24 hr post-injection. The expression of the kiss1, Kissr and Cyp19 genes was measured by RT-PCR. The results showed a significant increase in expression of the reproductive-related genes. Histological analysis revealed higher number of mature oocytes in kisspeptin treated groups compare to other ones. In conclusion, Hkiss and Kiss1+H are the most effective peptides in oocyte maturation and expression of reproductive-related genes. In addition, it seems that kisspeptins in other domestic animals can be used to stimulate the hypothalamus-pituitary-gonadal axis.  相似文献