Development and validation of an improved enzyme-linked immunosorbent assay for the detection of thyroglobulin autoantibodies in canine serum samples     

L Iversen  A.L Jensen  R Høier  M Skydsgaard  F Kristensen 《Domestic animal endocrinology》1998,15(6):525-536

An enzyme-linked immunosorbent assay to detect thyroglobulin autoantibodies (TGAB) in canine serum was developed and validated. The test result for each sample was derived from the optical density readings (OD) and expressed as an Ab-score(%) calculated from three in-house calibrators. The assay specifically detected TGAB as judged from lack of response in the assay after samples had been incubated with specific antigen. Intra- and interassay coefficients of variation ranged from 2.0–4.9% and 4.6–9.9%, respectively. The detection limit, an Ab-score of 5.6%, was close to the median Ab-score of 10% observed in healthy dogs (n = 132). The median Ab-score of dogs with primary hypothyroidism and lymphocytic thyroiditis (n = 11), skin diseases (n = 35), and non-thyroidal diseases (n = 63) was 340%, 12%, and 8%, respectively. The prevalence of TGAB in hypothyroid dogs with lymphocytic thyroiditis (sensitivity) was 91% (95% confidence limits: 59%–99%). In dogs with dermatological diseases without lymphocytic thyroiditis the prevalence of TGAB was 3% corresponding to a specificity of 97% (95% confidence limit: 85%–100%). In dogs with non-thyroidal diseases and healthy dogs the prevalence of TGAB was 5% and 6%, respectively. The diagnostic accuracy of serum TGAB was evaluated by subjecting the data from 11 dogs with lymphocytic thyroiditis and 35 control dogs without lymphocytic thyroiditis to receiver-operating characteristic curve analysis. The area under the receiver-operating characteristic curve (W = 0.966; 95% confidence limit 87%–100%) was significantly higher than that of a worthless test (0.5) (P < 0.0001), thereby indicating that serum TGAB measurements distinguished between dogs with and without lymphocytic thyroiditis.  相似文献   

添加苯二氮化合物F89对皖西白鹅生长和代谢性能的影响     

葛盛芳  葛继志  韩正康  陈杰 《中国家禽》1998,(10)

利用生理调节剂Ｆ８９提高皖西白鹅的摄食量,加速动物生长。日粮中添加２ｍｇ／ｋｇＦ８９,连续饲喂８～３５日龄皖西白鹅,日摄食量较对照组增加６．０２％,增重提高９．４０％,耗料比下降２．８９％。对育肥期鹅的增重也有明显影响。血液代谢物分析表明：饲喂Ｆ８９后,皖西白鹅血清中葡萄糖和尿酸浓度下降,β－脂蛋白浓度上升。提示Ｆ８９不仅能促进鹅的摄食量,而且可以影响鹅的代谢机能,促进生长  相似文献   

DETECTION OF PORTAL BLOOD FLOW USING PER-RECTAL ^99mTc-PERTECHNETATE SCINTIGRAPHY IN NORMAL CATS     

Fintan J.  McEvoy  MVB  PhD  Myra A. Forster-van  Hijfte  DVM  Robert N.  White  BSc  BVetMed 《Veterinary radiology & ultrasound》1998,39(3):234-237

This study reports data obtained from per-rectal ^99mTc-pertechnetate portal scintigraphy in normal cats. It examines the effects of chemical restraint and the methods employed in defining regions of interest (ROIs) on the shunt index derived from this data. Six normal cats were used for the study; all six were chemically restrained for imaging using propofol and later four of them were manually restrained for comparison. Portal blood flow was studied and the mean shunt index was found to be 5.9%± 3.9 when ROIs were operator defined and 9.2%± 4.4 when ROIs were defined using an isocontour program. In cats that were restrained using propofol and operator defined ROIs, the mean value for the time between detection of radioactivity in the liver and in the heart was 14 ± 1 seconds.  相似文献   

Comparison of Blood Physiological and Biochemical Parameters of Two Kinds of Minipigs in Different Altitudes     

GUO Xiao-yu  FENG Xiao-ming  LU Lu  WANG Hong-yi  NIU Ting-xian 《中国畜牧兽医》2015,42(6):1490-1494

This study was designed to compare the blood physiological and biochemical parameters between plateau Juema minipigs and plain Bama minipigs,and provide a theoretical basis for Juema minipigs served as experimental animals in the future.The blood samples of Juema minipigs were taken in Gannan,and the blood samples of the Bama minipigs were taken in Xi'an,24 blood physiological and biochemical parameters were tested using an automatic analyzer of blood cells and biochemistry.Between the plateau Juema minipigs and Bama minipigs,there were significant differences in PLT,RDW-CV and RDW-SD (P< 0.05) and extremely significant differences in RBC,HGB,HCT,MCH,MCMH,GGT,GLU and CRE (P< 0.01).The plateau Juema minipig had an altitude acclimatization,so it was better than Bama minipig in plateau medical science.  相似文献   

日粮钙水平对泌乳水牛生产性能的影响     

崔政安  邹彩霞  梁辛  韦升菊  李舒露  夏中生  梁贤威 《畜牧与兽医》2011,43(11)

为了探讨日粮钙水平对泌乳水牛生产性能的影响,本试验选用12头胎次、泌乳时间、泌乳量相近的水牛,随机分成4组,每组3头水牛。采用4×4拉丁方试验方法,4组水牛基础日粮中钙含量分别占基础日粮干物质的0.47%、0.62%、0.78%、0.93%,其他营养成分均相同,其中磷占采食干物质比例为0.50%;共4期试验,每一试验期21 d,其中预饲期7 d,正饲期14 d。试验结果表明:1、不同钙水平日粮对泌乳水牛的产奶性能影响不显著(P>0.05),但0.78%钙水平日粮组水牛的产奶量分别比其他3组提高5.4%、5.5%、3.9%,不同钙水平对水牛奶营养成分影响不显著(P>0.05)。2、不同钙水平日粮对水牛血浆中钙含量影响差异显著(P<0.05);随着日粮钙水平的上升,水牛血液甲状旁腺素和1,25二羟基维生素D呈显著下降趋势(P<0.05)。综合各指标,日粮钙占采食干物质的比例为0.78%能进一步提高泌乳水牛产奶量。  相似文献   

抓捕应激对鸡血清酶活性的影响研究   总被引：1，自引：0，他引：1  

邱伟海 《畜禽业》2007,(9):2-4

鸡受到抓捕应激后,开始血液中SOD活性降低,到第三天明显低于对照组;随着抓捕应激次数的增多,SOD活性又迅速升高,第十二天显著高于对照组,第九天、第十五天分别极显著高于对照组。CPK活性都高于对照组,而且随着应激次数的增加,CPK活性有逐渐上升的趋势。但抓捕应激对血清中GOT、GPT、APK、LDH、GSH-Px等酶活性的影响不显著。  相似文献   

杆菌肽锌对夏季产蛋鸡生产性能及血液指标的影响   总被引：1，自引：0，他引：1  

何欣  王晓霞  滑静  李健魁 《北京农学院学报》1999,14(3):34-38

３８０日龄北京白鸡８０只被随机分成２个处理，每个处理４个重复，每个重复１０只鸡，对照组喂基础日粮，试验组在基础日粮中添加５０ｍｇ／ｋｇ杆菌肽锌．试验被安排在１９９８年６月３０日至７月３０日进行．试验结果表明：杆菌肽锌对产蛋鸡平均产蛋量、平均蛋重及饲料转化率没有显著影响，但却可以明显改善蛋壳质量（蛋壳强度、蛋壳厚度）（Ｐ＜０．０５），并显著减少破软蛋率（Ｐ＜０．０１）．而且在夏季（３２℃以上）蛋鸡产蛋率下降阶段可以减少产蛋率的下降幅度．杆菌肽锌对蛋鸡血液中Ｃａ、Ｐ、Ｚｎ及ＡＬＫＰ（碱性磷酸酶）含量的影响不显著．  相似文献   

墨旱莲叶水提取物止血活性初探   总被引：3，自引：1，他引：2  

刘世旺  徐艳霞  徐霞玲  王二杏 《安徽农业科学》2008,36(31)

[目的]探讨墨旱莲叶水提取物的止血和促凝血活性,为中草药在止血方面的应用提供依据。[方法]将不同剂量的墨旱莲提取物溶液与抗凝兔血混合,测定凝血时间。[结果]墨旱莲叶提取物为0.5%的最适浓度时具有最强促凝血活性,凝血时间为16 s,凝血时间缩短率为99.6%。[结论]墨旱莲叶水提取物有显著的止血和促凝血活性,具有较高的开发利用价值。  相似文献   

茶多酚、茶多糖对兔动脉血压的影响   总被引：1，自引：0，他引：1  

马森 《畜牧兽医杂志》2009,28(1):3-4

兔静脉注10mg／mL的茶多酚和茶多糖溶液，观察对动脉血压的影响，结果表明：注射荼多酚1—3mL后收缩压6次降低（9％-20％），2次升高（13％和20％），3次无变化；舒张压2次升高（20％和140％），9次无变化；注射茶多糖1-4mL后收缩压9次降低（2％-29％），3次升高（13％-40％），5次无变化；舒张压4次降低（10％-200％），7次升高（20％-50％），6次无变化。上述浓度和剂量的茶多酚、茶多糖对兔动脉血压均无显著影响（P〉0．05—0．5）。  相似文献   

家兔氟乙酰胺中毒后血、尿指标的变化     

张小丽  马小军  陈怀涛 《动物医学进展》2007,28(1):14-16

为探讨动物氟乙酰胺中毒后血液和尿液指标的变化规律,将20只健康家兔随机分成4组,Ⅰ组为对照组,正常饲喂,Ⅱ、Ⅲ及Ⅳ组作为中毒组,分别经口灌服1.5、2.0、10.0 mg/kg体重的氟乙酰胺溶液(5.68×10-2 mg/L);各中毒组家兔死亡前从心脏采集血样,未死亡家兔和对照组家兔在试验第3天从心脏采集血样,进行红细胞、白细胞计数、白细胞分类计数和血红蛋白含量测定;通过膀胱穿刺采集尿液,用半自动生化仪测定亚硝酸盐、pH、葡萄糖、蛋白质、潜血、酮体、胆红素及尿胆素原等指标.结果显示,各中毒组家兔血红蛋白含量及红细胞总数较对照组显著降低(P＜0.05),白细胞总数显著增加(P＜0.05),其中淋巴细胞增加较为明显,中性粒细胞相对减少;各中毒组尿液中葡萄糖、蛋白质和尿胆素原含量较对照组都有所升高,pH显著降低(P＜0.05),尿中血红蛋白含量显著增加(P＜0.05).这些指标的测定可为动物氟乙酰胺中毒临床诊断提供参考.  相似文献