淡水鱼肝脏中维生素A1,A2含量的研究   总被引：5，自引：0，他引：5  

韩雅珊  广田才之 《中国农业大学学报》1998,3(3):17-20

本试验建立了用高效液相色谱法同时测定淡水鱼肝脏中的ＶＡ１和ＶＡ２的方法。以μ－Ｐｏｒａｓｉｌ３．９ｎｍ×１５０ｍｍ（Ｗａｔｅｒｓ Ｃｏ．）为色谱柱，正己烷：乙醚（８７：１３）为流动相，紫外３５０ｎｍ，荧光Ｅｘ３２５ｎｍ，Ｅｍ４８０ｎｍ双道检测，ＶＡ１和ＶＡ２的保留时间分别为２６．２５ｍｉｎ和２８．００ｍｉｎ。采用不同波长条件下的紫外吸收特性，对ＶＡ２予以定性，以ＶＡ１为内标物对ＶＡ２予以定量。实验  相似文献   

高职园林技术专业“1＋1＋1”工学结合人才培养模式研究   总被引：4，自引：1，他引：3  

黄顺  潘文明  唐蓉  尤伟忠 《现代农业科技》2009,(17):353-354

为了提高人才对社会需求的适应能力,以职业为导向,以能力为本位,以任务为载体,就苏州农业职业技术学院3年制高职园林技术重点专业建设为例,积极探索和研究工学结合人才培养模式在专业建设上的创新思路及主要实施途径。  相似文献   

Prokaryotic Expression of Brucella BAB Gene and Establishment of iELISA     

HANG Tianyu  ZHAO Hongzhe  SONG Qianjin  ZHANG Jing  JI Zhi  WEN Yongjun  GUAN Pingyuan 《中国畜牧兽医》2007,47(11):3641-3650

In this study,the genome of Brucella Rev.1 strain was used as a template to amplify the BAB gene sequence,clone it into the prokaryotic expression vector pET-30a(+),obtain the recombinant plasmid pET-30a-BAB,and perform prokaryotic expression on the recombinant plasmid.The indirect ELISA method was constructed by using the expression products detected by Western blotting.The results showed that the BAB gene was successfully cloned and expressed in this experiment,and the purified expression product was analyzed by SDS-PAGE.This study obtained a relatively pure recombinant BAB protein;Western blotting test showed that the expressed protein could react specifically with Brucella sheep positive serum and had good reactogenicity;Using the recombinant BAB protein as the coating antigen,an indirect ELISA method for detecting BAB antibodies was established and optimized.The best determined coating conditions were as follows BAB protein coating amount was 0.25 μg/mL,serum dilution was 1:400;blocking solution was 3% pig-derived gelatin;secondary antibody dilution was 1:6 000;color development time was 10 min.The established method was used to detect 40 clinical sheep serums,and the cut-off value was calculated to be 0.607.That was,when the serum tested had P/N ≥ 1.5 and D_{450 nm} ≥ 0.607,it was judged as positive,when D_{450 nm} ≤ 0.561,it was judged as negative,and when 0.607<D_{450 nm}<0.561,it was judged as a suspect value,and retest was required.Compared with the Huhong plate test and the test tube agglutination test,the positive coincidence rate was 100%,the negative coincidence rate was 71.88%,and the total coincidence rate was 77.5%.  相似文献   

复方中药提取物在新城疫B_1株免疫中的免疫增强作用     

陈德坤  李俊生  张宝川  程晓盈 《动物医学进展》1998,(3)

用自制的中药提取物与新城疫Ｂ１株混合后免疫５５日龄鸡，以血凝抑制试验测定新城疫抗体效价。结果表明，５５日龄鸡的中药提取物最佳用量为１．０ｍＬ，试验组鸡ＮＤ抗体效价明显高于对照组。免疫后６０ｄ用Ｆ４８Ｅ８新城疫强毒进行攻击，试验组的保护率达到１００％，而对照组相对较低。  相似文献   

Effect of F10 gene on activities of transcriptional factor NF-κB and AP1     

CAO Xiao-min  PANG Zhan-jun  QUAN Song  XING Fu-qi 《园艺学报》2008,24(5):901-904

  相似文献   

辣椒新品种齐杂尖椒1号的选育     

沃金荣  徐立民  刘继秀  王文华  谭漪 《中国蔬菜》2007,1(12):35-36

齐杂尖椒1号是以98-4-48为母本,以98-5-33为父本配制的辣椒一代杂种。生长势强,株高70cm,开展度55cm;果实长牛角形,纵径25~28cm,横径4.4cm,肉厚0.26cm;果面光滑,辣味中等,肉质脆,VC含量1.15g.kg-1,可溶性固形物4.97%;坐果率高,整齐度好,每667m2产量4000kg左右。适宜黑龙江地区种植。  相似文献   

野生大豆GsADF1基因的克隆与表达分析   总被引：1，自引：0，他引：1  

何慧  丁一琼  汪潇琳  喻德跃 《大豆科学》2009,28(5)

ADF蛋白是一种在真核生物中广泛存在的低分子量的肌动蛋白结合蛋白,在细胞分裂、细胞运动、植物顶端生长如花粉管伸长,根毛的形成等重要的生命活动中发挥着重要的作用.以野生大豆(Glycine soja)的叶片cDNA为模板,通过RT-PCR方法扩增分离获得GsADF1基因.该基因全长为693 bp,包含417 bp的开放阅读框;其编码的ADF蛋白含139个氨基酸残基.运用生物信息学方法将GsADF1与其他物种ADF蛋白氨基酸序列进行多重比对并构建系统进化树,发现GsADF1与其它生物的ADF蛋白具有很高的同源性.这说明植物ADF基因高度保守.为进一步研究GsADF1在野生大豆不同组织,器官以及不同发育期大豆种子中的表达情况,对它进行了荧光定量分析(Real time RT-PCR).结果表明:GsADF1在野生材料的根、茎、叶、花和种子中都有表达,且以根和花中表达量最高.而对其在不同发育阶段的种子中的分析表明该基因在15DAF、20DAF、30DAF、40DAF和45DAF的种子中都有表达,且以15DAF表达量最低,随后呈上升趋势.其表达量的变化表明GsADFI通过调节肌动蛋白可能参与了种子从胚胎发生到贮藏物质积累的转变.  相似文献   

The Correlation Between Polymorphisms of Exon1 and Exon4 of DRB1 Gene and Brucellosis in Kazakh Sheep     

MA Xuezhen  XU Jie  GAO Jianfeng  LI Gang 《中国畜牧兽医》2007,47(12):3844-3851

The purpose of this experiment was to study the correlation between exon 1 and 4 polymorphisms of DRB1 gene and brucellosis in Kazakh sheep.Using RBPT serological tests to try sheep serum,reference in GenBank sheep MHC Class Ⅱ area DRB1 gene sequences (Accession No.:NC_040271.1),the exon 1 and 4 pieces designed primers,using PCR-SSCP and DNA sequencing technology to 230 Kazak sheep DRB1 gene polymorphism detection,analyze its polymorphism loci and the relationship between the Kazak sheep Brucella susceptibility.The results showed that 66 Kazakh sheep were positive for Brucella in RBPT test,and the positive detection rate was 28.7%.There was one SNP locus (F1-G22A) in exon 1 fragment,and sequencing determined two genotypes (GG and GA),the dominant allele and genotype were G and GG respectively,and the susceptibility genotype of the polymorphisms of F1-G22A was GA.Chi-square test showed that there was no significant correlation between the polymorphisms of DRB1 gene F1-G22A and Brucella susceptibility in Kazakh sheep (P>0.05).According to the analysis of bioinformatics online software,the F1-G22A polymorphic sites lead to the change of RNA secondary structure and the decrease of minimum free energy,and lead to the change of protein secondary structure.No SNPs were found in DRB1 exon 4 fragment.Therefore,there might be a certain correlation between the polymorphisms of DRB1 gene F1-G22A and Brucella susceptibility in Kazakh sheep.  相似文献   

短蔓矮生型西葫芦新品种淄葫一号的选育   总被引：1，自引：0，他引：1  

王博  李炳华  王敬民  刘艳红  卢振宇  陈军 《农业科技通讯》2009,(11):179-180

淄葫一号是以自交系QS1-55为母本，以DL-31为父本配制而成的西葫芦一代杂种。该品种株型紧凑，生长势较强，早熟性好，雌花密，连续坐瓜性好，瓜条顺直，嫩瓜皮色淡绿，有光泽，商品性好。抗病毒病、霜霉病、白粉病能力比对照潍早一号强，露地栽培亩产量6000kg左右。适宜全国各地春秋露地及中小拱棚栽培。  相似文献   

西宁奶牛场玉米品种生产性能的分析     

崔凯 《青海草业》2004,13(4):12-13

通过对西宁奶牛场不同玉米大田的抽样分析,得出不同玉米品种的产量,营养价值及生长时间也不同,其中较好的品种主要有京多一号等.  相似文献