烟草病毒检测技术研究进展     

翟玉俊  刘红海  李星亮  黄伟  尼奇峰 《农产品加工．学刊》2009,(4)

近年来烟草病毒病发生病普遍,且日趋严重,对烟草的产量及质量影响很大,使烟草行业蒙受巨大损失。鉴于此,建立有效的病毒检测技术,对烟草病毒进行防治十分重要。综述了国内外对烟草病毒的检测技术,主要包括:指示植物法、电镜检测方法、血清学方法、分子生物学方法等。  相似文献   

抗卡那霉素单克隆抗体细胞株的筛选及间接ELISA法的建立   总被引：1，自引：0，他引：1  

齐红莉  杨广  刘金兰 《华北农学报》2010,25(2)

采用碳二亚胺(EDC)法合成卡那霉素完全抗原,免疫BALB/c小鼠,通过杂交瘤技术获得3株能稳定分泌抗卡那霉素单克隆抗体的杂交瘤(5D7、6H8、7G4)。选择其中的5D7株抗体,经ELISA鉴定,细胞上清的效价达103以上,腹水效价达到5×104以上;建立了间接竞争ELISA法,通过优化,其检测限为0.5ng/mL,抗体IC50为3.7ng/mL,与妥布霉素交叉反应率为63.8%,与其他抗生素无交叉。可见ELISA具有快速、敏感、特异、简便等特点,适合于KAN残留的快速检测,具有较高的推广应用价值。  相似文献   

Effect of ABA,arginine and sucrose on protein content of date palm somatic embryos     

Besma Sghaier  Walid Kriaa  Mouna Bahloul  Jesús V. Jorrín Novo  Noureddine Drira 《Scientia Horticulturae》2009

Abscisic acid (ABA), arginine and sucrose were evaluated for their effects on the morphology, germination rates and protein content of date palm somatic embryos (SE). Different concentrations of these supplements in the culture medium were used. The comparative study of SE length and thickness between treated and untreated SE revealed no differences, except for ABA (20 μM), which increased thickness. A decrease of water content (WC) in favor of an increase in dry weight (DW) was observed in all treated SE, especially with sucrose (90 g l⁻¹) and ABA (20 μM). Only ABA (20 and 4 μM) caused a proliferation rate of the cultures higher than those in the control. Although all the tested compounds increased protein content, ABA (20 μM) was more effective in protein enrichment than arginine and sucrose treatments. The SDS-PAGE protein profiles showed a significant difference between treated and untreated SE. A protein band of 22 kDa, identified as glutelin in a previous work, was accumulated after treatment with 20 μM ABA or 3 mM arginine. These findings may contribute to further understanding of the mechanisms involved in the accumulation of specific storage proteins in several plants.  相似文献   

Application of ELISA-based kit for detecting AOZ and determining its clearance in eel tissues     

Laddawan Krongpong  Kunihiko Futami  Takayuki Katagiri  Makoto Endo  Masashi Maita 《Fisheries Science》2008,74(5):1055-1061

Furazolidone, an antibacterial drug that was once widely used in the livestock industry and aquaculture, is now prohibited in numerous countries. It is difficult to detect residual furazolidone because it is readily metabolized in animal tissues but, by using and liquid chromatography coupled with tandem mass spectrometry, its metabolite, 3-amino-2-oxazolidinone (AOZ) can be detected. Here we describe the validity of an enzyme-linked immunosorbent assay (ELISA) kit to detect AOZ in Japanese eel Anguilla japonica tissue. ELISA is capable of detecting AOZ at 1.0 μg/kg in an eel sample with excellent accuracy and precision. Our results show that ELISA is suitable for regulatory purposes and for studying the fate of AOZ residues in eel treated with furazolidone. To measure the persistence of AOZ in eel tissues, eels (1.4–6.5g) were immersed in tanks containing 2 and 10 mg furazolidone/L for 3 h, and then maintained in a tank supplying well water for the next 160 days. The half-lives of AOZ, calculated from the linear terminal part of the excretion curve, were 25.0 days in muscle and 21.6 days in liver from fish exposed to 2 mg/L furazolidone. In the eels treated with 10 mg/L furazolidone, by contrast, high levels of AOZ were detected in liver and muscle, but the half-lives of AOZ were similar to those in fish treated with 2 mg/L furazolidone. The half-lives of AOZ in eel tissues were prolonged by the condition of low water temperature.  相似文献   

粮食污染物残留免疫分析技术研究与示范     

张少波  张文生  李文辉  陈嘉东  许艇  郑妙  张小松  刘志平  肖治理  刘剑锋  万红艳  黄金萍  田巍  葛俊  赵娜 《粮食储藏》2017,(4):5-14

随着人们对粮食安全问题的重视,政府部门不断加强对粮食安全的监测工作。针对粮食生产、加工、储运过程中的化学污染物安全性问题,研究粮食污染物快速监测技术,研究污染物免疫分析方法通过利用抗原、抗体的特异性反应来检测农药含量,设计并筛选吡虫啉、多溴联苯醚、功夫菊酯、氰戊菊酯4种半抗原,制备相关高特异性的抗体,分析吡虫啉、多溴联苯醚、功夫菊酯与气相色谱法(GC)相比,稻谷、大米、玉米样品通过相关性分析均高度相关,操作简便、快速。  相似文献   

BSA对牛结核抗体ELISA检测的影响   总被引：1，自引：0，他引：1  

吴庆儒  郭定宗  张桂荣  李文波 《动物医学进展》2007,28(5):5-9

分别研究了封闭液、血清稀释液及酶标二抗稀释液中BSA成分对牛结核抗体ELISA检测的影响.结果显示,酶标二抗稀释液中BSA的存在使ELISA检测值普遍偏低,且BSA的含量越高,检测值越低;为了提高ELISA检测值,使其达到理想检测效果,必须提高酶标二抗的使用浓度.BSA中可能存在的牛血清抗体杂质对酶标二抗起到了"中和"作用,降低了酶标二抗与目标抗体结合的实际浓度,使检测结果普遍偏低.  相似文献   

人乳铁蛋白转基因小鼠杂交选育的研究   总被引：1，自引：0，他引：1  

黄玉政  成勇  柏亚军  冯亚杰 《动物医学进展》2007,28(6):39-43

根据孟德尔遗传原理,对表达人乳铁蛋白转基因小鼠进行近交和远交,获得了纯合子小鼠(P品系1♀、Q品系1♀1♂)和双重杂合子小鼠(PQ杂合3♀3♂).催乳获得小鼠乳汁,ELISA检测乳汁中人乳铁蛋白的含量.通过比较发现,纯合子小鼠和单纯杂合子小鼠在表达量上没有明显差异,而双重杂合子小鼠的表达量则较前两者有明显提高.  相似文献   

枣树间接地下滴灌根区土壤盐分运移规律研究   总被引：7，自引：0，他引：7  

孙三民  安巧霞  蔡焕杰  姚宝林  乔英 《农业机械学报》2015,46(1):160-169

通过小区试验研究了间接地下滴灌条件下密植枣树根区不同方向(水平及垂直方向)、不同土层盐分质量比的变化特征,及导水装置直径(50、75、90 mm)和灌水量(9、13、17 L/(棵·次))对枣树根区土壤盐分运移的影响。结果表明:经过一段时间后,垂直方向10~40 cm土层盐分质量比较低,0~10 cm土层盐分质量比最高;水平方向10~50 cm各土层盐分质量比随与出水口水平距离的增加而增大。在单次灌水后导水装置直径一定时各土层脱盐率随灌水量的增加而增大。当灌水量一定时,10~40 cm土层脱盐率随导水装置直径的增加呈减小的趋势,0~10 cm、40~50 cm脱盐率随导水装置直径增大呈低-高-低的趋势。在7—9月份,当导水装置直径为75 mm,灌水量为13、17 L/(棵·次)时,0~50 cm土层土壤平均盐分质量比均较低而且变化幅度较小。综合考虑节水、抑盐效果,确定导水装置直径为75 mm,灌水量为13 L/(棵·次)为适宜的间接地下滴灌灌溉模式。  相似文献   

对生和互生玉米内源激素含量的差异分析     

马庆  程郢  齐璐璐  项艳  朱苏文  程备久 《农业生物技术学报》2008,16(6)

利用ELISA方法测定近等基因系玉米(Zeamays)对生、互生玉米的不同组织和不同生育期内源细胞分裂素ZRs(zeatin riboside group)、DHZRs(dehydro zeatin riboside group)、iPAs(isopentenyladenosine group)以及内源生长素IAA(indole acetic acid)含量差异。结果表明,不同叶序玉米在不同生育期幼胚、成熟花粉和成熟叶片的内源ZRs、DHZRs、iPAs和IAA均无显著差异;不同生育期对生叶序玉米幼苗顶端分生组织和成熟苗顶端雄幼穗ZRs、DHZRs和iPAs含量显著高于同期近等基因系的互生玉米,IAA含量在两者之间无显著差异;对生叶序玉米幼苗顶端分生组织、成熟苗顶端雄幼穗CTK/IAA(CTK表示ZRs、DHZRs和iPAs含量相加)比值显著高于同期近等基因系互生叶序玉米,但在不同生育期幼胚、叶片及花粉内均无显著差异。对生叶序的形成与生长点组织细胞分裂素含量增加密切相关。  相似文献   

血清法监测人工感染弓形虫病猪特异抗体的比较     

江涛  何会时  龚大春  陈声国  聂浩  姚宝安  赵俊龙 《湖北农业科学》2007,46(3):427-430

应用ELISA、LAT、IHA血清试验对8头人工感染弓形虫猪的血清抗体滴度变化规律进行了监测,并对不同检测方法的结果进行了比较.结果发现,试验猪在皮下感染弓形虫RH株速殖子后,血清中的特异性抗体首次检出的时间方面,LAT(第4 d)早于ELISA(第14 d)和IHA(第20 d);检出持续时间方面,LAT(54 d)长于ELISA(44 d)和IHA(19 d);平均检出率方面,LAT(7/8)高于ELISA(6.7/8)和IHA(5/8).试验结果表明,ELISA和LAT适合于猪弓形虫病的诊断和血清学调查,LAT更适合于猪弓形虫病的早期检测.  相似文献