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51.
52.
探索庐江地区不同栽培方式对油菜产量与经济效益的影响,在相同茬口条件下,以陕油28为试验材料,研究人工育苗移栽、机条播和无人机飞播等三种栽培方式对油菜生育特性、产量构成及经济效益等影响。结果表明,人工移栽产量最高,机条播经济效益最好,亦是目前农户主要栽培方式。作为规模生产主体,由于劳动力等因素,选择机条播为最佳。 相似文献
53.
【目的】对加州鲈弹状病毒弱毒三水株(MSRV-SS-7)及其母源株(MSRV-SS)进行全基因组克隆和测序分析。【方法】采用分段扩增方法对MSRV-SS-7及MSRV-SS基因组核心序列进行PCR扩增,第1轮根据鳜鱼弹状病毒(SCRV)基因序列(NC_008514.1)设计10对引物进行扩增并测序;在第1轮扩增产物测序、拼接基础上,设计8对引物进行第2轮扩增,拼接后获得基因组核心序列。同时用cDNA末端快速扩增法(RACE)获得3′和5′末端序列。采用Vector NTI 8.0对基因组核心序列和末端序列进行拼接,获得基因组全序列,使用Clone Manager 8.0对MSRV-SS-7和MSRV-SS进行序列比对分析;同时使用DNAMAN将基因组G基因序列转换为氨基酸序列,并通过MEGA 5.0与其他鱼类弹状病毒的G蛋白氨基酸序列进行进化树分析。【结果】MSRV-SS-7及其母源株MSRV-SS基因组全长均为11 548 bp,包含N、P、M、G、L等5个结构基因,基因组结构为3′Leader-N-P-M-G-L-Trailer 5′;MSRV-SS-7与MSRV-SS全基因组中共有10处核苷酸不同。G蛋白进化分析表明,MSRV-SS-7及母源株MSRV-SS与SCRV亲缘关系最近,且与鲈鱼弹状病毒属(Perhabdovirus)聚为一支。【结论】克隆分析了MSRV-SS-7的全基因组序列,可以用于后续活疫苗的开发。 相似文献
54.
《动物营养(英文)》2020,6(1):80-84
This experiment was conducted to investigate the effects of dietary lipid-to-carbohydrate ratio on growth and carbohydrate metabolism in juvenile cobia (Rachycentron canadum). Six isonitrogenous diets were prepared to vary in lipid-to-carbohydrate ratio (g/g) as follows: D1, 2.26; D2, 1.31; D3, 0.78; D4, 0.47; D5, 0.34; and D6, 0.23. Cobias were fed to satiety for 8 weeks. The weight gain and protein efficiency ratio in D1 group were significantly lower than those in other groups (P < 0.05), accompanied by a lower level of feed conversion ratio (P < 0.05). Protein retention efficiency in D4 and D6 and whole body protein in D4 and D5 were significantly higher than those in D1 group (P < 0.05). Survival rate in D4 group was the highest among all groups and was significantly higher than that in D1, D2 and D5 (P < 0.05). In terms of serum triglyceride, D1 and D2 were significantly higher than D6 (P < 0.05). Hepatosomatic index in D3 and D4 was significantly lower than that in D1 (P < 0.05). Fructose-1,6-diphosphatase in D4 was significantly higher than that in D1 and D3 (P < 0.05). Phosphofructokinase in D3 and D4 and malic enzyme in D4 and D5 were significantly higher than those in other groups (P < 0.05). Results indicate that cobia utilizes carbohydrates as energy source more efficiently than it utilizes lipids. The optimal lipid-to-carbohydrate ratio in juvenile cobia diets is 0.47. 相似文献
55.
Food resources and cyprinid diet in permanent and temporary Mediterranean rivers with natural and regulated flow 下载免费PDF全文
Carlos M. Alexandre Sabrina Sales Maria Teresa Ferreira Pedro R. Almeida 《Ecology of Freshwater Fish》2015,24(4):629-645
This study addresses the differences in food availability, diet and feeding activity of the Iberian barbel, between permanent and temporary nonregulated rivers, and the effect of flow regulation on feeding parameters. A total of 267 adult barbels were seasonally collected in four nonregulated and regulated rivers from permanent and temporary basins, and their gut content was analysed. Locally available food sources were evaluated across sites and seasons. Barbels from the permanent nonregulated river exhibit a more variable and diversified diet in which invertebrates assumed a large importance, especially during high flows. Barbels from the temporary nonregulated river presented a more uniform diet composed of plant material and detritus, particularly in drought seasons. Flow regulation affected different flow components in both systems, but the effects on food resources and barbels' diet were similar, resulting in an intra‐annual stabilisation of resource availability and fish diet, with a higher consumption of plants and detritus. Changes in fish diet and feeding activity in both nonregulated and regulated rivers were strongly associated with the seasonal variability of streamflow components, particularly between low‐ and high‐flow periods, and with the reduction in flow variability in the case of dam regulation. Results from this study can be used to improve guidelines for flow requirement implementation. 相似文献
56.
目的:从耗散结构理论的角度研究解决我国青少年体质健康普通下降的严重问题,对解决我国青少年体质健康问题不仅具有独特的理论价值,而且具有显著的实践意义。方法:理论思辨法和逻辑分析法。结论:1.青少年体质健康普通下降的重要原因是其系统的耗散结构无法形成;2.解决青少年体质健康问题的重要途径在于促进其系统的耗散结构形成;3.开放性,远离平衡与非线性是青少年体质健康系统形成耗散结构的前提;4.满足青少年自我实现的愿望和体育锻炼的需求是青少年体质健康系统能够形成耗散结构的关键。 相似文献
57.
Influences of population density,temperature and latitude on the growth of invasive topmouth gudgeon Pseudorasbora parva 下载免费PDF全文
Plasticity in life‐history traits provides advantages for introduced fish in overcoming demographic bottlenecks that would otherwise inhibit establishment. Here, the influence of population density, temperature and latitude was tested on the growth increments and growth rates of invasive populations of topmouth gudgeon Pseudorasbora parva, a small Asian cyprinid fish that is invasive across Europe. Aquaria experiments tested the roles of fish number and temperature on growth increments under a fixed food supply, pond experiments tested the role of density on growth increments, and a field study completed in England and Wales tested the influence of density and latitude on growth rates. In aquaria experiments, whilst growth increments were higher at 21 and 23 °C than at 19 and 25 °C, fish number had a greater influence on growth than temperature. Higher growth increments were produced at lower densities. In experimental ponds, growth increments were significantly higher in ponds with low densities of P. parva compared with those at elevated densities. In the field study comprising 10 wild populations across a latitudinal gradient of 4.0°N, a difference in mean air temperatures of approximately 3 °C, and estimated densities between 0.5 and 65.0 m?2, population density was the only significant predictor of growth rates. Whilst populations at very low densities comprised of significantly faster growing individuals, there were no significant differences when densities were between 15 and 65 m?2. Thus, invasive P. parva populations have considerable growth plasticity, especially at low densities, with this likely to be important in their ability to colonise new environments. 相似文献
58.
为探究氮素形态对菠菜生长和品质的影响,试验采用盆钵培养的方法,在总氮浓度为15 mmol/L的条件下,设置了5种NO_3~--N与NH_4~+-N配比(分别为100:0、75:25、50:50、25:75和0:100),测定了5种铵硝配比条件下菠菜生长与品质相关指标。结果表明:与NH_4~+-N相比,NO_3~--N更有利于菠菜生长与Vc积累。适当增加NH_4~+-N比例后,Vc含量虽显著降低,但对菠菜的生长却无显著影响,并可显著降低可食用部分硝酸盐含量。因此,为了提供高产、低硝酸盐的优质菠菜,笔者认为适宜的NO_3~--N?NH_4~+-N为50:50。 相似文献
59.
60.
苦瓜枯萎病是由尖孢镰孢菌苦瓜专化型(Fusarium oxysporum f. sp. momordicae)侵染引起的一种土传病害,目前市场上缺乏防治效果较好的农药或生防菌剂。为了更好地防治苦瓜枯萎病,通过盆栽试验筛选出绿针假单胞菌(Pseudomonas chlororaphis)G5 与恶霉灵复配施用的最优组合,并在苦瓜枯萎病发病样地对盆栽试验筛选出的最优组合进行防效验证。试验结果表明:菌药组合B+0.75Hym(1×109 cfu · mL-1 G5 菌悬液+187.5 mg · L-1 恶霉灵)处理对盆栽苦瓜枯萎病的防病效果为81.60%,明显高于其他处理;同时该组合对苦瓜幼苗的促生作用最为明显。接种苦瓜枯萎病菌后,菌药组合B+0.75Hym的苦瓜叶片中PAL、PPO、POD 防御酶活性较高。田间防效验证试验中苦瓜苗移栽30、60 d 后菌药组合B+0.75Hym 对苦瓜枯萎病的防治效果较高,分别达到了52.59%、32.13%,且苦瓜产量也高于其他处理。表明盆栽试验筛选出的菌药最优组合B+0.75Hym 不仅能够降低农药的使用量,而且能够有效防治苦瓜枯萎病,提高苦瓜产量,有一定的生产实用价值。 相似文献