Transcription of Endogenous Retrovirus Group K Members and Their Neighboring Genes in Chicken Skeletal Muscle Myoblasts     

Tomohide Takaya  Yuma Nihashi  Tamao Ono  Hiroshi Kagami 《The Journal of Poultry Science》2021,58(2):79

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噬菌体Bp7尾丝蛋白gp37的原核表达与鉴定     

孙虎芝  任慧英  张灿 《动物医学进展》2016,(5):10-14

为了研究噬菌体尾丝蛋白gp37在识别宿主菌大肠埃希菌K12过程中的作用,PCR扩增获得gp37基因C端1 161bp序列,以EGFP为荧光标签,构建重组表达质粒pET-28a-EGFP-gp37,在大肠埃希菌BL21(DE3)中IPTG诱导表达重组蛋白EGFP-gp37,亲和层析纯化蛋白。SDS-PAGE及Western blot检测结果表明,重组蛋白EGFP-gp37在大肠埃希菌BL21(DE3)为可溶性表达,表达量占菌体总蛋白量的23%。EGFP-gp37融合蛋白与大肠埃希菌K12相互作用,激光共聚焦显微镜检测结果显示,尾丝蛋白gp37能够吸附到宿主菌K12表面,表明尾丝蛋白gp37参与宿主菌受体的识别和吸附过程。  相似文献   

冬枣果园土壤非交换态钾的释放动力学     

王清华  杜振宇  马丙尧  刘方春  马海林 《中国农学通报》2015,31(10):221-225

为了研究长期施肥条件下冬枣果园土壤非交换态钾的释放特征,以Ca Cl2和柠檬酸溶液为浸提剂,采用连续提取的方法对枣园土壤的非交换态钾释放动力学进行研究。结果表明,枣园土壤非交换态钾在释放过程中表现出快速释放和缓慢释放2个连续的阶段。快速释放阶段(188 h)的累积释放量占到总释放量的53.6%~59.2%,而在缓慢释放阶段(188~1244 h)的释放量不及总量的一半。在相同时间内,使用柠檬酸溶液浸提时,非交换态钾的释放量明显高于使用Ca Cl2溶液时的释放量。相比之下,冬枣果园土壤非交换态钾的释放量明显高于对照土壤。在5种动力学方程中,幂函数方程是描述冬枣果园土壤非交换态钾释放的最优模型。  相似文献   

ICAI Realizaltion for the K-map Simplification of Logical Function     

CHEN Xin long  HU Guo qing 《保鲜与加工》2002,(10):149-151

Based on the theory of the K map simplification of logical function(KSLF),this article releases a ICAI Realization for KSLF.Providing three kinds of teaching style:commonness, teaching, practice.This product,wich supports for the K map simplification with random item,is not only helpful to the construction of the curriculum of digital circuit ,but also to the design for digital application system.Under the practice method, This product's guides,checks,and corrects the student's whole process forthe proceeding of KSLF automaticly under the teaching method.  相似文献   

液体射流泵空化噪声与振动试验     

龙新平  王磊 《排灌机械》2007,25(4):1-5

针对液体射流泵的空化噪声与振动进行了试验研究;运用B＆K加速度仪和水听器对射流泵各部位在不同工况下的空化噪声与振动进行了测量。结果表明：（1）射流泵空化噪声以单极子声源为主,随着流速和紊流度的增大,偶极子作用逐渐增大;（2）射流泵空化噪声与振动随空化程度加剧而更强烈,最强烈处也从喉管向扩散管转移;（3）射流泵面积比越小,达到临界空化前噪声频谱波动越强,临界空化之后噪声谱级趋于平稳,随频率增加而递减;（4）初生空化出现前后噪声在整个频域范围内都有一个较大的跃升,约5-10 dB,此跃升可作为判断初生空化的参考方法;（5）泵体3个正交方向振动并不相同,径向振动较轴向更为明显。  相似文献   

西南地区主要类型土壤钾素有效性分析     

朱启红  伍钧 《农机化研究》2007,(7):153-157

通过测定西南地区主要类型土壤的基本农化性状、Q/I曲线、固钾率和酸提取钾来研究土壤钾素缓冲容量(PBCK),进而分析土壤钾素的有效性.试验结果表明,PBCK与土壤CEC、固钾率和酸提取均呈极显著正相关,因此可以用PECK来衡量土壤钾素有效性.  相似文献   

Effects of naringenin on PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways in rats with myocardial ischemia/reperfusion injury     

LAN Zhuo  WANG Huan  HE Xi-song  YOU Li-ping  XU Nan  FENG Jian  LI Jia-fu 《园艺学报》2021,37(1):41-47

AIM To observe the effect of naringenin on cardiac injury in ischemia/reperfusion （I/R） rats, and to explore whether the role of naringenin is involved in PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways. METHODS SD rats （n=48） were randomly divided into sham operation （sham） group, model （I/R） group, naringenin treatment （NAR） group and naringenin+LY294002 （NL） group. Myocardial I/R injury model was prepared by ligation of left anterior descending coronary artery of rats for 30 min followed by reperfusion for 120 min. After reperfusion, the serum levels of cardiac troponin I （cTnI） was measured by ELISA. HE staining, TTC staining and TUNEL staining were used to detect the myocardial histopathological changes, myocardial infarction area and myocardial cell apoptotic rate. The mRNA levels of endoplasmic reticulum stress-related indicators glucose-regulated protein 78 （GRP78）, C/EBP homologous protein （CHOP） and caspase-12 were detected by RT-qPCR. The protein levels of cleaved caspase-3, GRP78, CHOP, caspase-12, p-PI3K and p-AKT were determined by Western blot. RESULTS Compared with I/R group, the serum content of cTnI, myocardial pathological damage, myocardial infarction area and myocardial cell apoptotic rate were significantly reduced （P<0.05）, the protein levels of cleaved caspase-3, GRP78, CHOP and caspase-12 were decreased （P<0.05）, and the protein levels of p-PI3K and p-AKT were increased in NAR group （P<0.05）. LY294002 attenuated the protective effect of naringenin to some extent. CONCLUSION Naringenin reduces myocardial I/R injury in rats possibly by activating PI3K/AKT signaling pathway and subsequently regulating endoplasmic reticulum stress and its related apoptotic pathways.  相似文献   

高亲和钾转运体在植物抗盐中的作用     

赵畅  张淼  李法莲  邵群 《安徽农业科学》2007,35(12):3470-3474

土壤盐渍化是导致作物减产的一项重要原因,Na+对大多数作物是有害的,而钾是植物不可缺少的营养成分,植物根系在外界低钾条件下主要通过高亲和性系统吸收K+.介绍了高亲和钾转运体Trk/HKT和KUP/HAK/KT 2大家族的结构和功能.主要讨论了当植物处于K+饥饿及盐胁迫下,2大家族在改善植物钾营养、提高植物耐盐性方面的作用.  相似文献   

黑线仓鼠胴体重长指标的研究   总被引：3，自引：0，他引：3  

叶华  卢浩泉 《四川农业大学学报》1997,15(2):180-184

黑线仓鼠（Ｃｒｉｃｅｔｕｌｕｓｂａｒａｂｅｎｓｉｓ）是华北平原最主要的农田害鼠之一，本文用胴体重代替体重采用数理统计方法，分析了其胴体重与体长之比（即胴体重长指标）的年龄、性别、季节、年度变化，结果除年度变化外，均发现有显著性差异，雄性的该值较雌性的大得多，同性相比，Ⅳ龄组最大，在１９８７１９８９年间的种群密度下，其胴体重长指标与种群密度间没有相关关系  相似文献   

Significance of salt transport patterns in rice varieties differing in salt tolerance     

《Communications in Soil Science and Plant Analysis》2012,43(1):63-69

Abstract

The absorption mechanisms for Na, K, SO₄ and Cl were tested in a salt tolerant (PVR 1) and a salt sensitive (GEB 24) rice varieties. The salt tolerant variety accumulated significantly larger amounts of Na than the salt sensitive variety. Further, PVR 1 absorbed SO₄ from Na₂SO₄ in preference to that from K₂SO₄. The absorption patterns for K and Cl were similar in both the varieties. It is concluded that the capacity of plant species to accumulate greater amounts of Na is a reflection of their halophytic feature.  相似文献