Artificial burrow preference by the Japanese crayfish Cambaroides japonicus     

Kazuyoshi Nakata  Tatsuo Hamano  Ken-Ichi Hayashi  Tadashi Kawai  Seiji Goshima 《Fisheries Science》2001,67(3):449-455

SUMMARY: Preference for artificial burrows by the endangered Japanese crayfish species Cambaroides japonicus was studied to improve its cultivation. The occupation of artificial burrows, which were made from straight polyvinyl chloride pipes of different internal diameters ( Y , mm), by crayfishes of different total lengths ( X , mm) was significantly ( P < 0.001, n = 56) described by a linear regression: Y = 0.49 X + 3.42 (19.0 ≤ X ≤ 70.2). Among burrows of different lengths [crayfish total length (TL) × 1, × 2, × 3, and × 4], crayfishes significantly preferred burrows that were greater than TL × 3 ( P < 0.001, n = 588).  相似文献   

Acanthochondria priacanthi (Copepoda: Chondracanthidae) as a biological indicator for stock identification of sandfish Arctoscopus japonicus (Steindachner)     

Takashi  YANAGIMOTO  Kenji  KONISHI 《Fisheries Science》2004,70(2):336-338

  相似文献   

Expression of a recombinant crustacean hyperglycemic hormone of the kuruma prawn Penaeus japonicus in methylotrophic yeast Pichia pastoris     

TSUYOSHI  OHIRA  HIDEKAZU  KATAYAMA  KATSUMI  AIDA  HIROMICHI  NAGASAWA 《Fisheries Science》2003,69(1):95-100

ABSTRACT:   Until now, six crustacean hyperglycemic hormones (CHH) designated Pej-SGP-I, -II, -III, -V, -VI and -VII have been characterized in the kuruma prawn Penaeus japonicus . All CHH consist of 72 amino acid residues and have an amidated carboxyl (C)-terminus. In the present study, we expressed Pej-SGP-III in methylotrophic yeast Pichia pastoris in order to obtain a large quantity of recombinant CHH possessing biological activity. A cDNA encoding Pej-SGP-III that had been previously cloned was processed by polymerase chain reaction (PCR) and the resulting product was ligated into an expression vector. Pichia pastoris was transformed with this vector after which a recombinant Pej-SGP-III was expressed having an additional amino acid residue (glycine) at the C-terminus (rPej-SGP-III-Gly), a form considered to be a putative precursor of this hormone. rPej-SGP-III-Gly secreted into the culture medium was purified by reversed-phase HPLC, and amidated using a peptidylglycine alpha-amidating enzyme. The amidated rPej-SGP-III (rPej-SGP-III) showed hyperglycemic activity in in vivo bioassay almost comparable to that of the natural Pej-SGP-III. rPej-SGP-III thus obtained will be a useful tool not only for its physiological study but also for the determination of its 3-D structure.  相似文献   

冬季室内刺参养殖水质管理方式试验     

许鹏  王志滨  周玮 《现代农业科技》2014,(4)

于2007年12月进行了冬季反季节养殖刺参的试验,结果表明:每天换水深度为40 cm的方式水质质量较好,是目前较可行的换水方式。  相似文献   

日本百脉根LjbHLH34基因克隆及耐旱功能鉴定     

刘福  陈诚  张凯旋  周美亮  张新全 《草业学报》2023,32(1):178-191

干旱是影响植物生长发育的重要环境因素。本研究分析了日本百脉根抗旱相关基因LjbHLH34的耐旱功能，初步解析其响应干旱胁迫的分子机制，以期为百脉根抗旱分子育种提供理论基础。本研究克隆得到的LjbHLH34基因大小为711 bp、编码236个氨基酸，属bHLH转录因子家族成员。系统进化树分析显示，LjbHLH34蛋白与拟南芥bHLHⅣ亚家族中AtbHLH34和AtbHLH104亲缘关系较近。实时荧光定量分析表明LjbHLH34在日本百脉根的根中表达量最高，叶中次之，茎中最少，暗示其在日本百脉根多个组织中发挥作用；同时LjbHLH34基因也受聚乙二醇(PEG)和脱落酸(ABA)诱导表达。在酵母中检测发现LjbHLH34具有转录激活活性；亚细胞定位试验表明LjbHLH34蛋白定位于细胞核中。将LjbHLH34基因转入拟南芥获得过表达株系。在200 mmol·L^-1甘露醇胁迫下，LjbHLH34转基因拟南芥的根长明显长于野生型。干旱处理后，野生型拟南芥比转基因拟南芥萎蔫程度更加明显，而转基因株系的相对含水量和超氧化物歧化酶(SOD)活性显著高于野生型，丙二醛(MDA)积累...  相似文献   

刺参与日本对虾池塘高效混养技术     

王秀云  何振平  刘艳芳  刘一鹤  王旭 《河北渔业》2008,(6):19-21

刺参夏眠期间正是日本对虾生长的大好季节,将刺参和日本对虾混养,在7月份放养虾苗,严格控制养殖池内的杂鱼虾等,就可大大提高日本对虾和刺参的成活率,利用此二者在饵料和空间的互补性,可显著提高虾池的利用率和经济效益。  相似文献   

养殖刺参保苗期重大疾病“腐皮综合征”病原及其感染源分析   总被引：20，自引：5，他引：20  

王印庚  方波  张春云  荣小军 《中国水产科学》2006,13(4):610-616

2003～2005年冬、春季,山东省和辽宁省众多养殖区域的保苗期刺参(Apostichopus japonicus)暴发了较为严重的传染性疾病-"腐皮综合征".该病波及面广,传染性强,死亡率常高达90%以上.发病症状主要表现为厌食、摇头、肿嘴、排脏、身体萎缩、口部溃烂乃至体表大面积溃疡.本研究对症状较为典型的3家海参保苗期的发病幼参进行了详细的病原学分析,从所有病参的病灶组织分离得到了1种占有绝对优势的菌株,经人工感染实验证明它对健康刺参具有较强的致病性,且感染病参的症状与自然发病海参的症状相同.通过形态学、生理生化和16S rDNA分子生物学方法对该菌进行的分类鉴定表明,导致保苗期刺参"腐皮综合征"的致病菌是假交替单胞菌(Pseudoalteromonas nigrifaciens).同时也对3家养殖场刺参保苗养殖系统进行了分析研究.结果表明,水源中细菌含量不高,为(0.8×102)～(1.2×102)cells/mL,其特征与病灶处优势菌不同;而饵料中细菌含量最高可达3.2×106 cells/mL.饵料、池水和池底污物的优势菌与病灶处优势菌基本一致,说明饵料可能是病原菌的主要来源.本研究首次揭示了"腐皮综合征"导致保苗期刺参大规模死亡的原因及其致病原,对刺参健康养殖和病害防治具有重要的指导意义.  相似文献   

浙南室内水泥池刺参度夏试验     

周朝生  胡利华  郑金和  张炯明  刘伟成  李小勇 《水产科学》2011,30(1):46-49

通过自然常温饲育和人工控温(23.6±0.5)℃的降温饲育,研究了3种规格体质量(10、20、50个/kg)刺参于浙江南部海区室内水泥池的度夏。结果表明,常温饲育下,3种规格的刺参体质量均呈负增长,且特定负生长率随着刺参体质量的增加而升高;降温饲育下,10个/kg与20个/kg体质量刺参组呈负增长,而50个/kg体质量刺参组略呈正增长,第30 d与第60 d的特定生长率分别为0.0496%与0.0513%。降温(23.6±0.5)℃饲育能有效提高刺参度夏时的存活率,有效降低刺参的特定负生长率,且刺参体质量越大,有效降低的体质量下降幅度也越大。在浙江南部海区室内水泥池养殖刺参,温度保持在(23.6±0.5)℃时,50个/kg的刺参不夏眠。  相似文献   

应用间接荧光抗体技术快速检测花鲈病原菌——鳗弧菌   总被引：7，自引：2，他引：7  

余俊红  姚斐  俞勇  纪伟尚  徐怀恕 《海洋水产研究》2002,23(2):38-44

以花鲈（Lateolabrax japonicus)弧菌病的病原菌-鳗弧菌（Vibrio anguillarum)W-1为抗原，制备兔抗血清；利用异硫氰酸荧光素标记的羊抗兔免疫球蛋白（FITC-IgG）为荧光标记二抗，并以罗丹明标记的牛血清白蛋白为背景染色，建立检测鳗弧菌的间接荧光抗体快速检测技术。应用该技术对人工感染后的花鲈组织（肌肉、鳃、肠、肾）样品和养殖水体样品进行了鳗弧菌检测，结果显示间接荧光抗体技术不仅可以用于诊断发病的感染花鲈，也可用于检测带菌状态或未发病的感染花鲈。  相似文献   

Effects of body size and water temperature on food consumption and growth in the sea cucumber Apostichopus japonicus (Selenka) with special reference to aestivation   总被引：7，自引：0，他引：7  

Hongsheng Yang  Xiutang Yuan  Yi Zhou  Yuze Mao  Tao Zhang  & Ying Liu 《Aquaculture Research》2005,36(11):1085-1092

To investigate the effects of body size and water temperature on feeding and growth in the sea cucumber Apostichopus japonicus (Selenka), the maximum rate of food consumption in terms of energy (C_maxe; J day^?1) and the specific growth rate in terms of energy (SGRe; % day^?1) in animals of three body sizes (mean±SE) – large (134.0±3.5 g), medium (73.6±2.2 g) and small (36.5±1.2 g) – were determined at water temperatures of 10, 15, 20, 25 and 30°C. Maximum rate of food consumption in terms of energy increased and SGRe decreased with increasing body weight at 10, 15 and 20°C. This trend, however, was not apparent at 25 and 30°C, which could be influenced by aestivation. High water temperatures (above 20°C) were disadvantageous to feeding and growth of this animal; SGRe of A. japonicus during aestivation was negative. The optimum temperatures for food consumption and for growth were similar and were between 14 and 15°C, and body size seemed to have a slight effect on the optimal temperature for food consumption or growth. Because aestivation of A. japonicus was temperature dependent, the present paper also documented the threshold temperatures to aestivation as indicated by feeding cessation. Deduced from daily food consumption of individuals, the threshold temperature to aestivation for large and medium animals (73.3–139.3 g) was 24.5?25.5°C, while that for small animals (28.9–40.7 g) was between 25.5 and 30.5°C. These values are higher than previous reports; differences in sign of aestivation, experimental condition and dwelling district of test animals could be the reasons.  相似文献