Effect of tetrandrine on radiosensitivity of nasopharyngeal carcinoma cells     

WANG Jun  CHANG Li-hong  LI Xia  CHEN Xian-zhen  WU Xi-fu  WANG Zhi-yuan  HUANG Zi-zhen  HUANG Jian-cong  ZHANG Ge-hua 《园艺学报》2017,33(9):1611-1618

AIM: To investigate the mechanism of the radiosensitizing effect of maximum non-cytotoxic doses of tetrandrine (Tet) on nasopharyngeal carcinoma cell lines CNE1 and CNE2.METHODS: The cells were treated with ma-ximum non-cytotoxic doses of Tet (for CNE1 cells at 1.5 μmol/L and for CNE2 cells at 1.8 μmol/L), irradiation at 4 Gy, or combination of irradiation and maximum non-cytotoxic doses of Tet. The cell cycle distribution was analyzed by flow cytometry. The protein levels of γ-H2AX, cleaved caspase-3, p-CDC25C, CDK1, p-CDK1, cyclin B1, ERK and p-ERK were determined by Western blot.RESULTS: The expression of γ-H2AX was increased in CNE1 cells and CNE2 cells after combined treatment with irradiation and maximum non-cytotoxic doses of Tet. The percentages of CNE1 cells and CNE2 cells at G₂/M phase in irradiation group were (18.09±0.42)% and (18.48±1.32)%, respectively, which were decreased to (15.88±1.04)% and (13.80±0.82)% in combined treatment group, respectively (P<0.05). Combined treatment enhanced the increase in the protein level of cleaved caspase-3 caused by irradiation. The protein levels of p-CDC25C and p-CDK1 were increased in a dose-dependent manner by Tet treatment (P<0.05), while the expression of CDK1 showed no difference among different doses of Tet treatments. The protein levels of p-CDC25C, p-CDK1 and CDK1 showed no difference after the treatment with maximum non-cytotoxic doses of Tet. The combined treatment with irradiation and the maximum non-cytotoxic doses of Tet decreased the protein levels of p-CDC25C and p-CDK1 (P<0.05), increased the expression of cyclin B1, and had no influence on the expression of CDK1 (P<0.05). The combined treatment resulted in an increase in the protein level of p-ERK1 (P<0.05).CONCLUSION: The maximum non-cytotoxic doses of Tet enhance the DNA damage and apoptosis in CNE1 cells and CNE2 cells caused by irradiation, and the mechanism might be associated with ending of G₂/M arrest via activation of ERK/CDC25C/CDK1/cyclin B1 pathways.  相似文献   

Investigation and genetic mapping of a Glomerella leaf spot resistance locus in apple          下载免费PDF全文

Yuanxia Liu  Jinhao Lan  Caihong Wang  Baohua Li  Jun Zhu  Chunxiao Liu  Hongyi Dai 《Plant Breeding》2017,136(1):119-125

Apple Glomerella leaf spot (GLS) is a severe fungal disease that damages apple leaves during the summer in China. Breeding new apple varieties that are resistant to the disease is considered the best way of controlling GLS. Fine mapping and tightly linked marker are critically essential for the preselection of resistant seedlings. In this study, a population of 207 F₁ individuals derived from a cross between ‘Golden Delicious’ and ‘Fuji’ was used to construct a fine simple sequence repeat (SSR)‐based genetic linkage map. The position of R_gls, a locus responsible for resistance to GLS, was identified on apple linkage group (LG) 15 using SSR markers CH05g05 and CH01d08, which was adapted from a published set of 300 SSR markers that were developed using the bulked segregant analysis (BSA) method. These two SSR markers flanked the gene, and its recombination rate was 8.7% and 23.2%, respectively. A total of 276 newly developed SSR markers around the target region and designed from the genome apple assembly contig of LG15 were screened. Only nine of these were determined to be linked to the R_gls locus. Thus, a total of 11 SSR markers were in linkage with R_gls, and mapped at distances ranging from 0.5 to 33.8 cM. The closest marker to the R_gls locus was S0405127, which showed a genetic distance of approximately 0.5 cM. The first mapping of the gene R_gls was constructed, and the locations of the 11 effective primers in the ‘Golden Delicious’ apple genome sequence were anchored. This result facilitates better understanding of the molecular mechanisms underlying the trait of resistance to GLS and could be used in improving the breeding efficiency of GLS‐resistant apple varieties.  相似文献   

Agronomic traits of soybean cultivars released in different decades after grafting record‐yield cultivar as rootstock          下载免费PDF全文

Shengyou Li  Fei Teng  Demin Rao  Xingdong Yao  Huijun Zhang  Haiying Wang  Shuhong Song  Steven K. St. Martin  Futi Xie 《Plant Breeding》2017,136(2):133-138

Breeders have seldom considered the selection for root traits during the genetic improvement in soybean. It is hypothesized that grain yield would be increased by the root function improvement, especially for the current cultivars. The objective of this grafting experiment was to determine the effect of record‐yield cultivars L14 or Z35 as rootstocks on agronomic traits of cultivars released in different decades. A total of 11 cultivars, released in different decades, were used to graft onto L14 or Z35 rootstocks. The agronomic traits were measured in the pot‐culture experiments. Grafting cultivars released in different decades onto L14 or Z35 rootstocks resulted in higher yield, 100‐seed mass and apparent harvest index as compared with those of non‐grafted or self‐grafted plants. Grain yield gain of cultivars grafted onto record‐yield cultivar rootstocks was 0.40 g/plant/year from 1966 to 2006, which was larger than that of non‐grafts and self‐grafts (0.27 g/plant/year). The yield of current cultivars should increase more if their root functions were improved.  相似文献   

GFDL-ESM2M气候模式下京津冀地区未来潜在蒸散量时空变化   总被引：3，自引：3，他引：0  

毕彦杰  赵晶  吴迪  赵勇 《农业工程学报》2020,36(5):141-149

为探究未来潜在蒸散量时空变化特征,该研究以京津冀地区为例,基于美国GFDL提供的GFDL-ESM2M全球气候模式,得到京津冀地区92个格点2000-2050年的平均气温、最高气温、最低气温、太阳总辐射、平均相对湿度和近地面平均风速,应用Penman-Monteith公式计算京津冀地区未来92个格点的逐日潜在蒸散量(ET0),分析其时空分布特征及其与气象要素的相关关系。结果表明:未来年ET0总体呈增加趋势,RCP8.5情景下ET0上升速度最快,且随着时间推移增幅越来越大。夏季ET0增长速度最快,其次为春季、秋季与冬季,意味着未来ET0季节差异将愈加明显,可能出现更为严重的季节性干旱。ET0空间分布呈由西南向东北逐渐递减趋势,其中中部地区增速最快,增长趋势由中部向南北递减。不同气候情景下平均气温均呈逐年上升趋势,风速、太阳总辐射略微上升,而相对湿度下降。ET0与太阳总辐射的相关系数最大,呈由东北向西南递增趋势,其次为最高气温,呈由西北向东南递增趋势。ET0与相对湿度变化呈显著负相关,相关系数绝对值呈东北向西南递增趋势,ET0与风速相关度不明显。该研究可为农业需水预测与灌溉管理、科学应对气候变化提供基础支撑。  相似文献   

TRMM在海河流域南系的降水估算精度评价及其对SWAT模型的适用性   总被引：3，自引：3，他引：0  

谭丽丽  黄峰  乔学瑾  刘海鹏  李春强  李保国 《农业工程学报》2020,36(6):132-141

准确估算区域降水对水文过程评价和水资源管理意义重大。为评估TRMM 3B42V7降水产品在海河流域南系的估算精度及其在土壤和水评估模型(Soil and Water Assessment Tool,SWAT)中的适用性,利用28个气象站降水观测数据(2007-2016年)和101个雨量站观测数据(2010-2016年)开展研究。研究表明:站点尺度上,3B42V7降水产品对月降水估算的均方根误差小于15 mm,平均误差小于8.5 mm;在湿润季节的估算精度更好。流域尺度上,日降水估算精度较差,相关系数小于0.6。分区尺度上,3B42V7能够很好地捕捉到不同等级降水强度,但对微量降雨有所低估;山区和平原的年降水量均出现高估现象,平原区较为突出;此外,3B42V7能够较好地捕捉到研究区内极端降水的时间和空间分布。分2种情景进行水文模拟,利用月平均流量对模型进行校准和验证,在情景Ⅰ中,验证期模拟结果较好,决定系数在0.56~0.96之间,纳什效率系数在-11.09~0.94之间。TRMM 3B42V7可为海河流域及其类似区域的水资源管理提供参考。  相似文献   

荷斯坦牛新遗传缺陷单倍型HH7分子筛查     

李艳华  吕小青  王丽云  刘林  麻柱 《中国奶牛》2021,(2):19-21

HH7(Holstein Haplotype 7)是在荷斯坦牛群中新发现的一种隐性遗传缺陷单倍型,由27号染色体上CENPU基因4个碱基缺失突变引起,隐性基因纯合时能引起胚胎早期流产,严重影响着奶牛养殖者的经济效益.本研究利用竞争性等位基因特异性PCR(KASP法)对随机抽取的166头荷斯坦公牛样本进行了检测分析,结果...  相似文献   

利用风洞实验研究~7Be示踪估算土壤风蚀速率的可行性     

孙喜军  杨明义  张风宝  张博  赵恬茵 《水土保持学报》2012,26(3):22-25,29

以黄土高原水蚀风蚀交错带沙黄土为研究对象,利用室内风洞模拟实验对7Be示踪估算土壤风蚀速率的可行性进行探讨。由于风蚀过程易带走土壤细颗粒,且7Be在土壤细颗粒中含量较高,所以利用7Be水蚀模型计算的土壤风蚀速率高于实测值。实验中发现样点风蚀后和风蚀前土壤颗粒比表面积之比与样点风蚀后7Be含量之间存在幂函数关系,基于此,提出颗粒校正系数(P)的计算式,并将P引入到7Be水蚀模型对其进行修正。计算分析发现,和实测值相比,利用修正模型计算的土壤风蚀速率误差均不超过5%,这说明经过修正的7Be水蚀模型能较准确地估算土壤风蚀速率,利用7Be示踪技术估算土壤风蚀速率是可行的。研究为进一步利用7Be示踪技术调查黄土高原水蚀风蚀交错带土壤风蚀问题奠定了基础。  相似文献   

猪繁殖与呼吸综合征病毒ORF7基因的表达和重组蛋白的纯化及免疫学活性分析   总被引：1，自引：0，他引：1  

张永富  韩春华  林健  刘月焕  韦海涛  祝俊杰  赵景义  李栋梁  马国文  布日额  李明刚  张婷  刘永宏  马明  张秋雨 《安徽农业科学》2009,37(10):4439-4442

[目的]在基因工程菌中实现猪繁殖与呼吸综合征病毒（PRRSV）ORF7基因的高效表达,对表达的重组蛋白进行纯化,并探讨其免疫学活性及应用价值。[方法]将已构建好的重组表达载体pET-ORF7转化大肠杆菌BL21（DE3）,用IPTG在最适条件下诱导表达,其表达产物用SDS-PAGE和Western Blot进行检测。应用Ni-NTAHis.Bind Resin层析柱在变性条件下纯化表达产物,通过梯度透析进行复性。采用Western Blot及间接ELISA法检测复性后重组蛋白的免疫学活性。[结果]重组质粒pET-ORF7在大肠杆菌中以融合形式成功表达,融合表达产物主要以包涵体形式存在。SDS-PAGE检测所表达的重组蛋白分子量为33 kD左右,与预期大小相符。Band-scan软件对表达蛋白分析发现,表达产物约占菌体蛋白的50%。Western Blot及间接ELISA法显示复性后的重组蛋白与PRRSV阳性血清有特异性结合反应,表明其具有良好的免疫学活性。[结论]该试验将为进一步研制PRRSV单克隆抗体及诊断试剂盒奠定基础。  相似文献   

60Co-γ射线辐射马铃薯块茎M2代群体的诱变效应   总被引：1，自引：0，他引：1  

李佩华  李世林  潘韬  王安虎  蔡光泽 《安徽农业科学》2009,37(27):13007-13009

[目的]研究60Co-γ射线的不同辐射剂量对马铃薯块茎M2代群体的诱变效应。[方法]以云薯2号和鄂马铃薯5号为试材,采用60Co-γ射线的4种辐射剂量（5、10、15、20GY）分别对2个马铃薯品种的休眠块茎进行辐射诱变,分别测量2个马铃薯品种M2代叶片的叶绿素含量、干物质、淀粉含量以及产量等农艺性状,研究60Co-γ射线的不同辐射剂量对马铃薯M2代群体的诱变效应。[结果]不同辐射剂量对同一马铃薯品种M2代主要农艺性状产生的变异程度不同;相同辐射剂量对不同马铃薯品种M2代主要农艺性状所产生的变异程度不同。60Co-γ射线诱变改良个别性状（早熟、抗病性、高产、叶绿素含量）较有效,60Co-γ射线诱变马铃薯的适宜照射剂量为10～20GY,从2个马铃薯品种M2代中共单选出41个优株系。[结论]对马铃薯进行辐射诱变是一种有效的育种手段,但大多数突变是隐性突变,辐射诱变育种难度较大。  相似文献   

Cr6+、Mn7+和Hg2+对青虾的毒性和联合毒性研究   总被引：5，自引：0，他引：5  

吕耀平  李小玲  贾秀英 《上海水产大学学报》2007,16(6):549-554

探讨了Cr6 、Mn7 和Hg2 三种重金属离子对青虾(Macrobrachium nipponense)的急性毒性及联合毒性作用。结果表明:Cr6 对青虾的24、48、72、96h的半致死浓度LC50分别为8.937、5.001、3.484、2.241mg/L;Mn7 对青虾24、48、96 h的LC50分别为45.497、34.191、13.293、2.460mg/L;Hg2 对青虾的24、48、72、96 h的LC50分别为0.0415、0.0239、0.0168、0.0131mg/L。急性毒性由强至弱依次为:Hg2 >Cr6 >Mn7 。Cr6 -Hg2 对青虾96 h的联合作用表现为协同作用;Mn7 -Hg2 对青虾的96 h联合急性毒性表现为:低浓度的Mn7 对Hg2 表现为协同作用,低浓度的Hg2 对Mn7 表现为拮抗作用;Mn7 -Cr6 96 h联合急性毒性表现为:高浓度的Mn7 对Cr6 表现为拮抗同作用,而非低浓度的Cr6 对Mn7 均表现为协同作用。并就Cr6 、Mn7 和Hg2 对青虾的急性致毒效应特征、安全浓度以及重金属离子间联合毒性效应进行了分析与探讨。  相似文献