分子印迹技术在兽用抗生素残留分析中的应用进展     

王晓通  李臣贵 《中国兽药杂志》2021,55(9):50-56

随着规模化养殖业发展,兽用抗生素大量使用,不合理甚至违法添加使用导致的残留现象已成为威胁动物源食品安全的重要因素,迫切需要建立灵敏可靠的残留分析方法。从复杂的生物样品中提取纯化微量的残留药物是制约兽药残留检测方法建立的关键。分子印迹技术具有特异性强、灵敏度高、操作简便等优点,在兽药残留检测研究中得到越来越多重视。文章主要综述了分子印迹聚合物的最新制备方法及在兽用抗生素残留分析中的最新研究进展。  相似文献   

A Homozygous RAB3GAP1:c.743delC Mutation in Rottweilers with Neuronal Vacuolation and Spinocerebellar Degeneration          下载免费PDF全文

T. Mhlanga‐Mutangadura  G.S. Johnson  A. Ashwini  G.D. Shelton  S.A. Wennogle  G.C. Johnson  K. Kuroki  D.P. O'Brien 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(3):813-818

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Australian Cattle Dogs with Neuronal Ceroid Lipofuscinosis are Homozygous for a CLN5 Nonsense Mutation Previously Identified in Border Collies          下载免费PDF全文

A. Kolicheski  G.S. Johnson  D.P. O'Brien  T. Mhlanga‐Mutangadura  D. Gilliam  J. Guo  T.D. Anderson‐Sieg  R.D. Schnabel  J.F. Taylor  A. Lebowitz  B. Swanson  D. Hicks  Z.E. Niman  F.A. Wininger  M.C. Carpentier  M.L. Katz 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(4):1149-1158

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稻米营养品质的研究现状及分子改良途径   总被引：18，自引：0，他引：18  

江良荣  李义珍  王侯聪  黄育民 《分子植物育种》2004,2(1):113-121

本文综述了稻米营养品质(蛋白质、赖氨酸和微量营养素)的经典遗传学和分子遗传学、分子育种的研究现状，并对利用分子标记辅助育种方法提高稻米营养品质作了展望。  相似文献   

甘蓝型油菜SRAP、SSR、AFLP和TRAP标记遗传图谱构建   总被引：27，自引：0，他引：27  

金梦阳  刘列钊  付福友  张正圣  张学昆  李加纳 《分子植物育种》2006,4(4):520-526

以黄籽GH06为母本、黑籽P174为父本杂交得到的第6代重组自交系188个株系为作图群体,通过SRAP、SSR、AFLP和TRAP四种分子标记对该群体进行遗传连锁分析,构建了一张包含20个连锁群、300个标记位点的甘蓝型油菜分子遗传图谱(LOD≥3.0),包括202个SRAP标记、65个SSR标记、23个AFLP标记和10个TRAP标记。图谱总长度1273.7cM,标记间平均距离为4.25cM。连锁群上的标记数在4～56个之间,连锁群长度变动在37.1～109.2cM之间,群内平均图距在1.80～14.20cM之间。LG1包含的标记最多,有56个;标记最少的连锁群(LG9、LG18、LG20)只有4个。LG13的平均图距最大,为14.20cM;LG6的平均图距最小,仅为1.80cM。在整个图谱上,存在图距大于20cM的空隙6个。本研究首次将SRAP及TRAP标记用于甘蓝型油菜遗传图谱的构建,结果表明,SRAP及TRAP标记在甘蓝型油菜遗传图谱的构建上是一种良好的标记系统。  相似文献   

犬瘟热病毒F蛋白分子生物学研究进展   总被引：2，自引：0，他引：2  

许红丽  易立  王建科  杨莘  程世鹏 《特产研究》2011,33(2):52-56

犬瘟热病毒融合蛋白是囊膜糖蛋白,是产生中和抗体的主要保护性抗原,结构相对保守。融合蛋白介导病毒囊膜和细胞膜融合,决定病毒在宿主体内扩散的能力,在病毒的致病性及免疫原性上具有重要作用。因此,对犬瘟热融合蛋白基因及其蛋白的结构和功能进行研究具有重要的意义。本文从蛋白结构、蛋白功能、核酸序列比对等多方面对融合蛋白进行了阐述。  相似文献   

The first survey and molecular identification of Entamoeba spp. in farm animals on Qinghai-Tibetan Plateau of China     

《Comparative immunology, microbiology and infectious diseases》2021

Protozoans of Entamoeba spp. are globally distributed zoonotic parasites that infect diverse animal hosts and humans. Prevalence and species/genotypes distribution of Entamoeba spp. in domestic animals are not fully investigated on Qinghai-Tibetan Plateau (QTP), an animal husbandry and agriculture region of China. In a survey, 528 fecal samples were collected from 7 species of domestic animals on multiple locations across QTP region and analyzed by PCR and sequencing analysis. The overall prevalence of Entamoeba spp. infection in all examined animals was 97.9 %. Four Entamoeba species, E. bovis, E. moshkovskii, E. ecuadoriensis and E. histolytica were found, and majority (94.2 %) of Entamoeba-infected animals harbored E. bovis. Six Entamoeba genotypes, Entamoeba ribosomal lineages (RL) 1, 2, 3, 4, 8 and 9 were identified by sequencing analysis. Two zoonotic species, E. moshkovskii and E. histolytica, were present in horses, while E. ecuadoriensis and E. bovis were found in horses and all species of seven farm animals, respectively. It was also observed that six Entamoeba genotypes were distributed in animals in specific pattern. The results revealed high prevalence of Entamoeba spp. infection in livestock, broad range of hosts as well as diversity and species/genotype distribution of Entamoeba spp. in farm animals inhabiting on the high altitude QTP region.  相似文献   

Genotyping of Mycobacterium bovis by geographic location within Mexico   总被引：1，自引：0，他引：1  

Milián-Suazo F  Banda-Ruíz V  Ramírez-Casillas C  Arriaga-Díaz C 《Preventive veterinary medicine》2002,55(4):e129-264

The spacer oligonucleotide typing (spoligotyping) method was used to differentiate 62 Mycobacterium bovis isolates obtained from tissues with macroscopic lesions typical of tuberculosis in dairy cattle from different regions of Mexico. Our purpose was to see if a strain from one region was genetically different from those of other regions (with the long-term aim of doing molecular trace back of isolates obtained in the laboratory). Results from the genetic analysis indicate that M. bovis isolates cannot be grouped by geographic location due to a wide range of genetic types involved in dairy cattle infections. Isolates even from the same herd showed different spoligotypes but some isolates from different region had similar genetic patterns. Genetic typing without epidemiologic information does not seem to be a plausible method to trace back animals to source of origin to detect and eliminate sources of infection.  相似文献   

Imported case of equine rabies in Finland: Clinical course of the disease and the antigenic and genetic characterization of the virus     

A.E. Metlin DVM  R. Holopainen MSc  S. Tuura  C. Ek-Kommonen DVM  A. Huovilainen PhD 《Journal of Equine Veterinary Science》2006,26(12):584-587

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潍坊萝卜中芜菁花叶病毒分离物的分子特性及CP基因的表达   总被引：1，自引：0，他引：1  

刘金亮  于晓庆  田延平  都杰  竺晓平  李向东  严敦余 《园艺学报》2006,33(1):84-87

 从表现红心病的潍坊萝卜块根上得到一芜菁花叶病毒( Turnip m osaic virus, TuMV) 分离物(TuMV-Ra) , 通过RT-PCR获得了该分离物的外壳蛋白(CP) 基因, 对其进行了序列测定, 并将其核苷酸序列及推导出的氨基酸序列与GenBank中登录的其它20个TuMV萝卜分离物的相应序列进行比较和分析。结果表明, TuMV-Ra与这20个分离物CP基因核苷酸序列的同源性为89.9% ～99.0% , CP氨基酸序列同源性为94.8%～99.7%; 其中与中国浙江杭州萝卜的两个分离物HZLB1、HZLB2同源性最高, 核苷酸序列的同源性为98.7%和99.0% , 氨基酸序列同源性为99.0%和99.7%。TuMV-Ra与1999年从表现相同症状的潍坊萝卜得到的分离物CHINA-WF CP基因核苷酸同源性为93.66% , 氨基酸同源性为96.53% , 说明病毒发生了比较大的变异, 而且变异的位点主要在CP的N末端。将TuMV-Ra CP基因克隆到原核表达载体pET-22b ( + ) , 在大肠杆菌BL21 (DE3) 中表达出分子量为38 kD的融合蛋白。Western blotting分析证明TuMV-Ra CP在大肠杆菌中得到了正确表达。  相似文献