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81.
Intramuscular injections of compounds of low irritancy (bicillin, valium, saline) were found to cause significant elevations in serum CPK in dogs. This route of treatment must therefore be avoided in clinical conditions where CPK values have diagnostic or prognostic importance.  相似文献   
82.
AIM:Hydroxymethylglutaryl CoA (HMG-CoA) reductase inhibitors, such as simvastatin, have been shown to reduce atherosclerotic cardiovascular morbidity and mortality by mechanisms unrelated to its lipid-lowering effect. Several studies have shown that simvastatin induces apoptosis in a varieties of cell lines including vascular smooth muscle cells (VSMC). The aim of this study was to investigate the signal pathways involved in apoptosis induced by simvastatin.METHODS:Cultured VSMC were treated with simvastatin. Calpain activity was determined by measuring Ca2+ ionophore-specific calpain substrate (suc-LLVY-AMC), caspase-3 activation was detected by Western blot, and apoptotic changes were distinguished by annexin Ⅴ binding and DNA laddering.RESULTS:After incubated with 30 μmol/L simvastatin for 8 h, calpain activity had a marked increase (P<0.05, n=4) and reached to more than 3-fold of control at 12 h (P<0.01). Caspase-3 also activated by simvastatin after 12 h. PD150606, a cell-permeable selective calpain inhibitor, decreased simvastatin-induced apoptosis rate from 24.2%±1.7% to 9.5%±1.9% (P<0.01) and also prevented simvastatin-induced DNA laddering. Furthermore, 100 μmol/L PD150606 efficiently inhibited simvastatin-induced caspase-3 activation.CONCLUSION:Simvastatin induces apoptosis by activating caspase-3 via calcium-dependent protease calpain.  相似文献   
83.
AIM: To evaluate the alterations in calcium metabolism of the vascular smooth muscle in the late phase of septic shock and test the hypothesis that nitric oxide might be involved in sepsis-induced vascular hyporeactivity. METHODS: Male Sprague-Dawley rats were subjected to sepsis by cecal ligation and puncture (CLP). 18 hours post CLP, rat aortic rings were employed for measurement of contractile responses by using organ bath technique. RESULTS: In endothelium-denuded aortic rings from CLP rats, concentration-contraction curves to phenylephrine (PE) and KCl were significantly decreased when compared to that from sham control rats. The transient contraction induced by PE in calcium-free Krebs solution and the concentration-dependent contraction to CaCl2 in KCl-depolarized medium were also markedly reduced. The hyporeactivity was partially reversed by treatment with aminoguanidine, a selective inducible nitric oxide synthase inhibitor. CONCLUSION: An impairment in calcium handling in vascular smooth muscle is involved in the vascular hyporeactivity during the late phase of septic shock, in which an excessive nitric oxide production might be the major mechanism.  相似文献   
84.
85.
Recombinant bovine somatotropin (bST) was administered s.c. to lambs for 6 weeks to evaluate its effects on fattening performance, carcass composition and visceral organ weights. Twenty male Karayaka lambs were injected with 250 mg bST every two weeks. Body weight, live weight gain, feed intake and feed conversion rate were not affected by treatment. Head, feet, skin, liver, spleen, kidneys, filled and empty gastrointestinal tract, penis, testis, pelvic fat and internal fat weights were similar for the two groups. Lung weight increased by 17.4% (P < 0.01) and weight of heart decreased 22.7% (P < 0.05) in bST treated lambs. The only carcass characteristics that were affected by bST administration were limb muscle which increased from 1331 g to 1417 g (P < 0.05), loin fat which decreased from 275 g to 174 g (P < 0.01), shoulder fat which decreased from 26 g to 13 g (P < 0.01) and total fat/final weight percentage which decreased from 9.8% to 7.6% (P < 0.05). The results of this study show that prolonged release formulation of bST treatment reduces fat tissue, but does not significantly affect fattening performance or weights of visceral organs, muscle and bone.  相似文献   
86.
刺猬肌肉、脂肪品质及营养成分分析   总被引:1,自引:0,他引:1  
对野生动物刺猬(Erinaceuseuropaeus)肌肉、脂肪的品质及营养成分进行器官及理化检验。结果表明,刺猬肌肉含蛋白质202%,脂肪8%,矿物质1%,熟肉率612%,肌纤维直径1895±345μm,脂肪熔点20℃~30℃,碘价94~99,皂化价170~175。肌肉的品质及营养成分可与常用动物肉(猪、牛、羊、鸡、兔肉)相媲美;油脂与常用动植物油脂相比,感官指标良好,脂肪酸不饱和程度明显高于动物油脂,而与花生油等多种植物的不饱和程度相当。  相似文献   
87.
β-激动剂克伦特罗在猪肝脏和肌肉组织中的残留   总被引:8,自引:0,他引:8  
本文报道用高效液相色谱法检测β 肾上腺素能激动剂(克伦特罗Clenbuterol)在猪肝脏和背最长肌中的残留量。在肥育猪日粮中添加3mg/kg克伦特罗,试验期30天,停药0、1、2、3、4天屠宰取肝脏和肌肉样。组织经匀浆浓缩提取,色谱条件为:CLC ODS色谱柱;以20mmol/LKH2PO4+30μmol/LEDTA(pH3.9)乙腈=8218(V/V)为流动相;紫外检测波长为243nm。结果表明,克伦特罗最低检测限为2ng/g。停药当天(0天)肝脏和肌肉组织残留量分别为208.5ng/g和10.0ng/g。停药后残留量迅速下降,肌肉在停药后第2天即检测不出,而肝脏要到第4天才检测不出。  相似文献   
88.
美济礁附近海域3种金枪鱼肌肉成分检测与营养评价   总被引:1,自引:0,他引:1  
为分析中国南海美济礁附近海域大目金枪鱼(Thunnus obesus)、蓝鳍金枪鱼(T.thynnus)、黄鳍金枪鱼(T.albacares)的营养成分与营养价值,该研究采用国标法检测了3种金枪鱼的水分、蛋白质、灰分、脂肪酸和氨基酸成分及其结构,并进行营养评价.结果显示,3种金枪鱼中黄鳍金枪鱼蛋白质含量最高,蓝鳍金枪鱼...  相似文献   
89.
[目的]克隆家蚕副肌球蛋白/小副肌球蛋白基因,分析该基因与运动性状的关系。[方法]利用PCR扩增和RACE技术获得了家蚕(Bombyx mori)副肌球蛋白的全长cDNA和小副肌球蛋白的部分cDNA;经检索家蚕wgs(基因组散弹序列)数据,获得了家蚕副肌球蛋白/小副肌球蛋白的基因组序列,并确定其基因组结构。[结果]家蚕PM/mPM基因由17个外显子和16个内含子组成,通过基因内部选择性启动子的使用,该基因组序列同时编码副肌球蛋白和小副肌球蛋白,小副肌球蛋白和副肌球蛋白共用最后6个外显子。家蚕副肌球蛋白与其他无脊椎动物副肌球蛋白的聚类分析表明,家蚕和野桑蚕有最近的亲缘关系,在昆虫纲中与黑腹果蝇关系最远。[结论]家蚕和野桑蚕副肌球蛋白的氨基酸序列间不存在可引起飞行缺陷的点突变,推测家蚕和野桑蚕飞行能力差异存在其他调控机制。  相似文献   
90.
3种养殖模式下大黄鱼肌肉营养成分比较及品质评价   总被引:1,自引:0,他引:1  
为探明大型深水抗风浪网箱养殖大黄鱼与海区放养和传统普通网箱养殖大黄鱼的肌肉品质差异,测定了3种养殖模式下大黄鱼的一般营养成分、氨基酸、脂肪酸、矿物质和微量元素组成。结果表明,海区放养大黄鱼粗蛋白含量高于深水网箱,显著高于普通网箱养殖大黄鱼(P0.05)。普通网箱养殖大黄鱼粗脂肪含量是海区放养大黄鱼的2倍以上,深水养殖大黄鱼的粗脂肪含量介于二者之间。海区放养大黄鱼的脯氨酸含量高于深水网箱养殖大黄鱼,高于普通网箱养殖大黄鱼(P0.05);丙氨酸、蛋氨酸和色氨酸的含量差异不显著(P0.05);海区放养组其他氨基酸含量与深水网箱组差异不明显,显著高于普通网箱组(P0.05)。脂肪酸含量排在前6位的分别是棕榈油酸、棕榈酸、油酸、硬脂酸、DHA、EPA,放养大黄鱼和普通网箱养殖大黄鱼棕榈酸含量最高,深水网箱大黄鱼的油酸含量最高。不饱和脂肪酸含量依次为自然组(63.60)、深水网箱(66.32)、普通网箱(57.67),多不饱和脂肪酸含量依次为29.1、28.57、24.40,海区放养养殖大黄鱼DHA含量显著高于深水网箱和普通网箱养殖大黄鱼。3种养殖模式下锌含量差异不明显,海区放养和深水网箱大黄鱼的磷含量相同,高于普通网箱。海区放养钙和镁含量均高于深水网箱高于普通网箱。深水网箱硒含量和普通网箱养殖大黄鱼相当,低于海区放养大黄鱼。该研究对于不同养殖模式下大黄鱼肌肉品质及不同养殖来源的大黄鱼鉴定具有一定的应用价值。  相似文献   
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