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干旱胁迫对白桦实生幼苗叶片蛋白质的影响 总被引:9,自引:0,他引:9
The studies on leave protein of Betula platyphylla seedlings by the methods of SDS-PAGE electrophoresis and dimensional gel electrophoresis was concerned. The result indicated that some new proteins such as 29kD pI4.3, 29kD pI4.2, 14kD pI5.3 and 11.5kD pI5.16 protein point were induced by soil drought stress, and some protein disappeared,for instance four protein between 12.4 - 11.5kD pI5.8 - 6.44 in seedlings leaves of B. platyphylla during soil drought stress. 相似文献
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K. Hylland C. Haux C. Hogstrand K. Sletten R. A. Andersen 《Fish physiology and biochemistry》1994,13(1):81-91
One isoform of the low-molecular-weight metal-binding protein metallothionein (MT) has been isolated from the liver of Atlantic
cod by size-exclusion and ion-exchange chromatography. Cod MT contained 33% cysteine, no aromatic amino acids or arginine.
As is the case for other piscine MTs, the N-terminus of cod MT lacked the asparagine in position 4 which is present in mammalian
MTs. In addition, cod MT differed from all other vertebrate MTs described in that the N-terminal methionine was not acetylated.
Antibodies were raised in rabbits against hepatic MT from cod by repeated injections of native protein mixed with adjuvant.
Anti-cod MT antisera cross reacted with similarly-sized proteins in liver, brain, anterior kidney, posterior kidney, spleen,
intestine, gills and ovaries. The putative MT in cod brain migrated differently to that of the other tissues in native gel
electrophoresis. Intraperitoneally injected Cd (1 mg/kg) was nearly entirely associated with the MT-peak in hepatic and renal
cytosols, whereas a single injection of Zn (10 mg/kg) resulted in increases in all cytosolic Zn pools of the liver and no
apparent change in cytosolic Zn, Cu, Ni or Cd in kidney. 相似文献
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[目的]探讨仔猪血清运铁蛋白多态性与生产性能的相关性。[方法]采用垂直平板聚丙烯酰胺凝胶电泳法对60头仔猪血清运铁蛋白多态性进行了研究,并对血清运铁蛋白多态性与生产性能进行了相关性分析。[结果]被检仔猪血清运铁蛋白有2种基因型(Tf^AB和Tf^88),以TfBB为优势基因型,基因型频率分别为0.3333和0.6667;TfA和Tf^B等位基因频率分别为0.1667和0.8333,以’畔为优势基因。仔猪血清运铁蛋白多态性与生产性能间无显著相关(P〉0.05)。[结论]仔猪血清运铁蛋白存在多态性,且血清蛋白多态性与生产性能间无显著相关。 相似文献
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Loss of genetic variation may render populations more vulnerable to pathogens due to inbreeding depression and depletion of variation in genes responsible for immunity against parasites. Here we review the evidence for the significance of variation in genes of the Major Histocompatibility Complex (MHC) for conservation efforts. MHC molecules present pathogen-derived antigens to the effector cells of the immune system and thus trigger the adaptive immune response. Some MHC genes are the most variable functional genes in the vertebrate genome. Their variation is clearly of adaptive significance and there is considerable evidence that its maintenance is mainly due to balancing selection imposed by pathogens. However, while the evidence for selection shaping MHC variation on the historical timescale is compelling, a correlation between levels of MHC variation and variation at neutral loci is often observed, indicating that on a shorter timescale drift also substantially affects MHC, leading to depletion of MHC diversity. The evidence that the loss of MHC variation negatively affects population survival is so far equivocal and difficult to separate from effects of general inbreeding. Some species with depleted MHC variation seem to be particularly susceptible to infection, but other species thrive and expand following severe bottlenecks that have drastically limited their MHC variation. However, while the latter demonstrate that MHC variation is not always critical for population survival, these species may in fact represent rare examples of survival despite of the loss of MHC variation. There is clearly a compelling need for data that would disclose the possible consequences of MHC diversity for population viability. In particular, we need more data on the impact of MHC allelic richness on the abundance of parasites or prevalence of disease in populations, while controlling for the role of general inbreeding. Before such evidence accumulates, captive breeding programs and other conservation measures aimed at inbreeding avoidance should be favoured over those protecting only MHC variation, especially since inbreeding avoidance programs would usually conserve both types of genetic diversity simultaneously. 相似文献
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聚丙烯酰胺凝胶电泳(PAGE)是实验室常用的基本鉴定方法之一。但由于做胶方式以及各实验室仪器设备良莠不齐,经过一系列纷繁复杂的操作,电泳结果未必尽如人意。其中凝胶带型的判断就是一个较为困惑的问题,一般的聚丙烯酰胺胶跑出的条带总体上都有弧度,越是靠近凝胶两侧越是严重。在本实验中,我们设计了一种较为简单易行的方案,即将难以区分的条带样品做成混合样marker来辅助辨型,通过调整电压和凝胶浓度来达到区分条带的目的。我们用该方案可以将相差0~3bp大小的条带区分开来。 相似文献
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