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Limited effects of land use on soil dissolved organic matter chemistry as assessed by excitation–emission fluorescence spectroscopy and molecular weight fractionation 下载免费PDF全文
P. Roberts J. M. Gibbons P. W. Hill D. L. Jones M. Farrell 《Soil Use and Management》2016,32(4):662-665
Dissolved organic matter (DOM) in soil solution represents a complex mixture of organic molecules and plays a central role in carbon and nitrogen cycling in plant–microbial–soil systems. We tested whether excitation–emission matrix (EEM) fluorescence spectroscopy can be used to characterize DOM and support previous findings that the majority of DOM is of high molecular weight (MW). EEM fluorescence spectroscopy was used in conjunction with MW fractionation to characterize DOM in soil solution from a grassland soil land management gradient in North Wales, UK. Data analysis suggested that three distinct fluorescence components could be separated and identified from the EEM data. These components were identified as being of humic‐like or fulvic‐like origin. Contrary to expectations, the majority of the fluorescence signal occurred in the small MW (<1 kDa) fraction, although differences between soils from the differently managed grasslands were more apparent in larger MW fractions. We conclude that following further characterization of the chemical composition of the fluorophores, EEM has potential as a sensitive technique for characterizing the small MW phenolic fraction of DOM in soils. 相似文献
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The following paper presents results of studies on the atomization process of water in pressure-swirl atomizers with different shapes of orifice using a laser diffraction technique. The effect of the shape of the orifice and the injection pressure on the droplet size distributions and mean droplet diameters was analyzed. The results obtained showed that droplet size decreases with an increasing injection pressure and droplet size distributions are expanded. The smallest droplet diameters were obtained by atomization in profiled and conical shaped orifice atomizers, however the largest droplet diameters were achieved for plain orifice atomizers. The analysis of the interfacial surface produced by the atomizers has shown that the greater enhancement of the atomization process had occurred using profiled and conical shaped orifice atomizers compared with plain orifice atomizers. The correlation for the Sauter mean diameter as a function of the Reynolds number and the ratio of orifice length to diameter has been proposed. The paper is related to agricultural water management. The data obtained are important from the point of view of the design of atomizers, agricultural treatments and crop protection. 相似文献
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In cucumber, the genetic basis of traits under domestication and/or diversifying selection is not well understood. Here, we reported QTL mapping for flowering time and fruit size-related traits with segregating populations derived from a cultivated × wild cross. Phenotypic data of flowering time (FT), fruit size (FS), fruit number (FN) and fruit weight per plant (FW) were collected in multiple environments. QTL analysis identified 19 QTL for these traits. We found that the major-effect QTL FT1.1 played an important role in regulating flowering time in cultivated cucumber, whereas the minor-effect QTL FT6.3 contributed to photoperiod sensitive flowering time during domestication. Two novel consensus FS QTL, FS1.4 and FS2.3, seem to be the targets of selection during breeding for the US processing cucumber. All other FS QTL were co-localized with previously detected QTL using populations derived from cultivated cucumbers, suggesting that they were under selection during both initial domestication and subsequent improvement. Results from this study also suggested that the wild cucumber is a useful resource for capturing positive transgressive segregation and novel alleles that could be explored in cucumber breeding. 相似文献
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分别从工艺流程、结构和工作原理以及使用中的注意事项等方面介绍了刨花计量料仓在刨花板生产线中施胶前及铺装前的应用情况。 相似文献
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The total ammonia nitrogen (TAN) removal efficiency and bacterial community composition of bioflocs with <50-μm particle size, > 50-μm particle size and un-sieved bioflocs were investigated in the current study. The initial ratio of dissolved organic carbon to TAN (DOC/TAN) in the three groups were about 14:1. No significant difference was found in the removal rate of TAN, average concentrations of TAN and nitrite nitrogen among the three groups (P > 0.05). The C/N (w/w) ratio of the > 50-μm bioflocs was significantly higher than those of the other groups. No significant differences were found in the crude protein content in the bioflocs among the three groups. The development of the bacterial community compositions of the bioflocs was analyzed by Illumina MiSeq sequencing analyses. Most OTUs were shared among the three groups at all the sampled time points. With the increase in the relative abundance of phylum Firmicutes, that of phylum Proteobacteria, Chorolexi, and Bacteroidetes decreased in all the three groups. The phylum Firmicutes and genus Bacillus were predominant in all the sampled time points. At the end of the experiment, genus Bacillus accounted for 81% in the < 50-μm group, 82% in the > 50-μm group, and 75% in the un-sieved group. 相似文献
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前期研究中发现了一个果肉低表达而叶片中高表达的荔枝基因FKBP16-2。本文克隆了该基因1 578 bp的启动子片段并对其功能进行了初步分析,结果表明:荔枝FKBP16-2基因启动子序列中含有大量的TATA-box和CAAT-box保守元件,以及TCA-element,ARE,HSE,GCN4_motif,O2-site等各种转录调控相关的顺式作用元件。该启动子能驱动GUS基因在荔枝的花、叶、根、果皮以及种子中表达而在果肉中不表达,表达具有组织特异性。 相似文献
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为探究基于A矩阵期望遗传关系最大化(maximizing the expected genetic relationship for matrix A,RELA)、基于A矩阵目标群体遗传方差最小化(minimized the target population genetic variance for matrix A,MCA)、平均亲缘关系最大化(the highest mean kinship coefficients,KIN)、随机选择(random selection,RAN)、共同祖先筛选(common ancestor,CA)等不同参考群筛选方法及参考群规模对基因型填充准确性的影响。本研究使用矮小型黄羽肉鸡作为试验群体,采用鸡600K SNP芯片(Affymetrix Axion HD genotyping array)进行基因分型,测定435羽子代公鸡45、56、70、84、91日龄体重。利用Beagle软件将低密度SNP芯片填充为高密度SNP芯片数据,比较不同参考群筛选方法、参考群规模对基因型填充准确性的影响,以及填充芯片基因组预测准确性。结果表明,使用Beagle 4.0结合系谱信息进行填充效果最佳,其次为Beagle 4.0,而Beagle 5.1填充效果最差。使用MCA方法筛选参考群进行基因型填充准确性最高,使用RAN方法筛选参考群进行基因型填充准确性最低,MCA、RELA、CA 3种方法基因型填充准确性差别较小。相比其他方法,使用MCA方法筛选个体作为参考群将低密度SNP芯片填充至高密度SNP芯片进行基因组选择的预测准确性较高,与真实高密度SNP芯片的基因组预测准确性相差甚微。随着参考群规模增大,基因型填充准确性也随之增加,但增速逐渐下降,最后趋于平缓。综上所述,可以通过参考群筛选方法构建参考群以及控制参考群规模,以保证基因型填充和基因组预测准确性并节省成本,本研究为基因型填充在畜禽遗传育种中的应用提供技术参考。 相似文献
30.
分析探讨口感化及颗粒化开食料对早期断奶羔羊生长和胃肠道发育的影响。选取新生健康的双羔湖羊公羔42只,随机分为两组,分别饲喂颗粒化和口感化开食料,试验期42 d。两组羔羊21日龄之前的采食量、体重和绝对生长的变化趋势相似。21日龄以后,口感化开食料组羔羊的采食量、体重、绝对生长和相对生长均明显高于颗粒化开食料组,42日龄体重、后两周的采食量及15~21日龄的相对生长率在两组间有显著差异(P < 0.05)。口感化开食料还显著提高(P < 0.05)了羔羊42日龄时的育肥指数、体长指数、胸围指数、管围指数和断奶前的瘤胃质量和瘤胃/胴体。以上结果表明,与颗粒化开食料相比,口感化开食料更有利于羔羊早期断奶前后的瘤胃发育、采食量提高、体重和体尺的发育,但对其他胃室及肠道发育没有影响。 相似文献