生育相关性抗原对精子受精的影响     

戈新  李培培  王建华  张宝珣 《安徽农业科学》2012,40(20):10452-10454

生育相关性抗原对精子获能、受精具有重要作用,已经被认为是精子受精潜能的标志。该抗原降解精子-中性粒细胞结合物,使精子重获自由,提高受精精子比例。综述了FAA的生成、结构、化学性质及其与精子受精的相关性,以期为将其作为精液稀释剂的组分奠定基础。  相似文献   

Stallion Sperm Selection: Past,Present, and Future Trends     

Jane M. Morrell 《Journal of Equine Veterinary Science》2012

Although several selection techniques are available for processing spermatozoa, only colloid centrifugation has been used to any extent in this field, starting with density gradient centrifugation and progressing more recently to single-layer centrifugation (SLC). SLC through a species-specific colloid has been shown to be effective in selecting spermatozoa with good motility and normal morphology from stallion semen. The method is easier to use and less time-consuming than density gradient centrifugation, and has been scaled-up to allow whole ejaculates to be processed in a practical manner. The potential applications of SLC in equine breeding are as follows: to improve sperm quality in artificial insemination doses for “problem” ejaculates, to increase the shelf life of normal sperm doses, to remove pathogens (viruses, bacteria), to improve cryosurvival by removing dead and dying spermatozoa before freezing or after thawing, to select spermatozoa for intracytoplasmic sperm injection, and to aid conservation breeding.  相似文献   

向日葵品种对锈病抗性的组织学和超微结构研究     

景岚  王丽芳  康俊  韩青梅  康振生 《中国油料作物学报》2013,35(3)

为了进一步了解向日葵与向日葵锈病病菌的互作关系及品种的抗性机制,采用电子显微镜技术对向日葵品种对锈病抗性的组织学和超微结构进行研究.在电镜下观察发现,向日葵锈病菌侵染向日葵后,在感病品种和抗病品种上发育的组织学和超微结构特征有明显差异.病菌在感病品种细胞间隙分布有大量的胞间菌丝,寄主细胞发生质壁分离;叶绿体变形,叶绿体的片层结构排列零乱,直至叶绿体解体.在抗病品种上表现为菌丝生长受抑,没有观察到吸器的产生,寄主细胞的早期坏死与锈菌的发育受阻密切相关.品种的抗病性可能与饥饿阻止真菌生长有关.  相似文献   

Ultrastructure of Fusion Product between Protoplasts from C3 and C4 Species of Amaranthaceae     

《Plant Production Science》2013,16(1):67-74

Summary

This paper describes the ultrastructure of the electric field-induced fusion products of C₃ and C₄ species of Amaranthaceae at the early developmental stage. Protoplasts of C₃ species were isolated from a Ceiosia cristata L. cell suspension and, those of C₄ species were isolated from an Amaranthus tricolor L. cotyledon. Incompatibility occurred in the C₃/C₄ hybrid. The incompatibility reactions were detected in the newly formed hybrid cells accompanied with significant changes in the nucleolus (segregation of nucleolar components) and plastids (cup-like shape or amoeboid plastid enclosing cytoplasmic materials) of C₃ species parent. The structural changes in the organelles of the C₄ partner were less marked. After 5 days of culture, most organelles showed high cellular activity, and a normal dedifferentiation process of mesophyll chloroplasts was observed. At this stage nucleolar segregation was not detected and the C₃ species plastids were difficult to distinguish from the proplastids formed from mesophyll chloroplasts. In addition, some mitochondria showed bursting-like structure. However, under the culture condition used these somatic incompatibility did not seem to impair further growth of fusion products since they were still proliferating well resulting in callus formation.  相似文献   

Effects of Pentoxifylline on Equine Epididymal Sperm     

Priscilla N. Guasti  Gabriel A. MonteiroRosiara R. Maziero  MSc  Ian MartinBruno R. Avanzi  MSc  José A. Dellaqua Jr.Frederico O. Papa  PhD 《Journal of Equine Veterinary Science》2013

This study evaluated whether pentoxifylline (PTX) present in the flushing extender influenced the function of equine epididymal spermatozoa after recovery and after thawing. For this experiment, 58 testicles from 29 Brazilian Jumping Horses were used. Cauda epididymides of each stallion were separated and flushed with a skim milk extender, with or without 7.18 mM PTX and then subjected to the freezing process. Samples flushed with the extender containing PTX showed a significant increase in total motility, progressive motility, straight line velocity, curvilinear velocity, and percentage of rapid sperm immediately after the recovery of epididymal sperm and after 15 minutes of incubation at 37°C (P < .05). However, the presence of PTX in the flushing extender did not affect the post-thaw motility parameters or plasma membrane integrity (P > .05). The results of this study showed that the PTX present in the flushing extender improved motility parameters of recently recovered epididymal sperm and had no deleterious effects on plasma membrane integrity and freezability of equine epididymal sperm.  相似文献   

A Comprehensive Pathological Survey of Duodenal Biopsies from Dogs with Diet‐Responsive Chronic Enteropathy     

Y‐M. Chang  C. Downes  S. Miller  K. Stevens  K. Verheyen  A.D. Phillips  S. Miah  M. Turmaine  A. Hibbert  J.M. Steiner  J.S. Suchodolski  K. Mohan  J. Eastwood  K. Allenspach  K. Smith  O.A. Garden 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2013,27(4):862-874

  相似文献   

Effects of Salinity Stress on the Seminal Root Tip Ultrastructures of Rice Seedlings (Oryza sativa L.)     

《Plant Production Science》2013,16(2):103-111

Abstract

Growth and structural changes in the seminal root tip of rice seedlings (Oryza sativa L. cv. Nipponbare) in response to NaCl salinity were studied. Seedlings were grown in agar medium with 0, 0.1, 0.3, 1.0, 2.0 and 3.0% NaCl(agar culture), and in water with 0, 0.01, 0.03, 0.06 and 0.1% NaCl (water culture). Seedling growth was significantly suppressed by higher concentrations of NaCl. The effect of NaCl appeared faster in water culture than in agar culture. In both agar and water cultures, root growth was markedly suppressed over shoot growth. Under saline conditions, epidermis, cortex and root cap cells appear to be damaged to a greater extent than the meristem and stelar cells. The most notable ultrastructural change in response to salinity was the development and increment of vacuoles, which seem to provide a space for accumulation of excess ions. Many electron dense deposits were observed in the larger vacuoles of the epidermal and cortical cells. Under saline conditions, cell wall thickening of the epidermis, an increase in endoplasmic reticulum, myelin figures, less compact Golgi bodies and inhibited production of Golgi vesicles were also observed.  相似文献   

杉木球果果鳞受顶枯拟盘多毛孢侵染后细胞结构变化的电镜观察     

曾思海 《福建林业科技》1998,25(4):30-32

杉木球果受顶枯拟盘多毛孢（Ｐｅｓｔａｌｏｔｉｏｐｓｉｓａｐｉｃｕｌａｔｕｓ（Ｈｕａｎｇ．）Ｈｕａｎｇ．）侵染后,罹病果鳞细胞的超显微结构依侵染程度不同表现一系列病理变化,主要表现在：叶绿体的基粒片层和膜结构遭到破坏;线粒体内嵴结构和膜结构遭到破坏;基质的许多细胞器解体消失。  相似文献   

小麦同核异质雄性不育系花粉发育的超微结构   总被引：3，自引：0，他引：3  

李传友  孙兰珍 《华北农学报》1998,13(1):24-29

对小麦８３（２１）３５核背景下三种细胞质雄性不育系（Ｔ,Ｖ,Ｋ）及保持系的花粉发育过程进行了超微结构研究,发现Ｔ型和Ｖ型不育系花粉败育前和败育中,液泡膜处于解体状态,细胞质和液泡间的有效分隔被破坏,液泡对细胞质的吞噬细胞强烈,线粒体等细胞器解体或退化,Ｖ型不育系花绒毡层内表面缺乏乌氏体分泌,在Ｋ型不育系花粉败育前,没有观察到线粒体,质体等细胞器结构方面的异常变化,但见到一种同心圆复合膜状结构以很高  相似文献   

干旱对不同耐性玉米品种光合作用及产量的影响   总被引：31，自引：2，他引：29  

陈军  戴俊英 《作物学报》1996,22(6):757-762

３个不同耐性玉米品种豫玉３号、豫玉１号和新黄单８５－１,在生育中后期遭受干旱胁迫７天后,产量均明显降低,其中以开花期干旱减产幅度最大。品种之间以不耐旱品种新黄单８５－１产量下降最严重。干旱处理后,各品种单株叶面积减少,干物质降低,雌穗发育不良。叶片的光合作用随水分胁迫的加重迅速降低,叶绿素含量明显减少,叶肉细胞的超微结构遭到不同程度的损伤。  相似文献