Gill epithelium of an angler catfish,Chaca chaca (Siluriformes,Chacidae): Enzyme and glycoprotein histochemistry     

Arup Mistri  Usha Kumari  Swati Mittal  Ajay Kumar Mittal 《Anatomia, histologia, embryologia》2020,49(1):67-79

A series of histochemical techniques have been employed to localize alkaline phosphatase, acid phosphatase, non-specific esterase, catalase and peroxidase; and to visualize and characterize glycoprotein (GPs) moieties in the epithelium of gill arch, gill filaments and secondary lamellae of an angler catfish Chaca chaca. The epithelium of gill arch and gill filament shows strong alkaline phosphatase activity in the deeper layer epithelial cells; strong non-specific esterase activities in the outer layer epithelial cells; and weak acid phosphatase activity throughout the epithelium. The activity of these enzymes in the secondary lamellae is weak. The catalase and peroxidase show strong activities in the blood cells of the secondary lamellae. Various classes of GPs have been identified and characterized in the mucous secretions of the gill epithelium of C. chaca. These include—GPs with oxidizable vicinal diols, GPs with sialic acid residues without O-acyl substitution and GPs with O-sulphate esters. The functional significance of different enzymes in gill epithelium and the GPs in the mucus secreted on the surface has been discussed with the physiology of the gills in relation to the characteristic habit and habitat of the fish.  相似文献   

Histopathological changes on the gills of asp (Aspius aspius) and European catfish (Silurus glanis) caused by Lamproglena pulchella and a Lamproglena sp. (Copepoda: Lernaeidae), respectively          下载免费PDF全文

K Molnár  A Avenant‐Oldewage  B Sellyei  Á Varga  C Székely 《Journal of fish diseases》2018,41(1):33-39

In a parasitology survey of Hungarian fishes, heavy infections of parasitic copepods Lamproglena pulchella and a Lamproglena sp. were found in the gills of the asp and the European catfish, respectively. Individuals of both fish species were emaciated and infected with hundreds of Lamproglena. Copepods located close to the tip of gill filaments and formed a depression at the attachment sites. In histological sections, cell degenerations and local haemorrhages were present adjacent to the maxillipeds and where the maxillary claws pierced the gill tissue. Around maxillae and in the midgut of the Lamproglena, damaged piscine blood cells and remains of the gill tissue were observed. Host reaction was expressed by proliferation of epithelioid cells, increase in both number and size of goblet and mast cells and formation of giant cells.  相似文献   

Seasonal patterns in the community of gill monogeneans on wild versus cultured orange‐spotted grouper,Epinephelus coioides Hamilton, 1822 in Daya Bay,South China Sea     

Luo Yufa  Yang Tingbao 《Aquaculture Research》2012,43(8):1232-1242

Epinephelus is among the most important fish genera in the Southeast Asian Pacific coastal systems. In Chinese coast, the orange‐spotted grouper Epinephelus coioides is one of the most significant species based on culture volume, and it is consumed all over the world. Very little information exists on this species’ parasitofauna. Composition and temporal variation in the community structure of the gill monogeneans on wild versus cultured E. coioides from Daya Bay (23°25′N, 117°2′E), South China Sea were determined using seasonal samples taken between April 2008 and January 2009. Eight species of monogeneans of three families and four genera on the gills of E. coioides were found: Neobenedenia melleni, Haliotrema cromileptis, Diplectanum grouperi, Pseudorhabdosynochus justinei, P. lantauensis, P. coioidesis, P. serrani and P. shenzhenensis. Eight of the monogenean species exhibited seasonal variation in their infection dynamics associated with environmental changes during seasons. The variations in the infection dynamics generated changes in the community structure during the sampling periods. Moreover, in the three different host living environments (wild, monocultured and polycultured), the monogenean communities exhibited the different seasonal patterns.  相似文献   

Effects of azithromycin on tilapia (Oreochromis niloticus): health status evaluation using biochemical,physiological and morphological biomarkers          下载免费PDF全文

Natália Sayuri Shiogiri  Cynthia Venâncio Ikefuti  Silvia Patricia Carraschi  Claudinei da Cruz  Marisa Narciso Fernandes 《Aquaculture Research》2017,48(7):3669-3683

Bacterial diseases cause tilapia's high‐mortality outbreak. This study investigated the toxicity of azithromycin (AZT), a macrolide antibiotic that has been considered a possible therapeutic drug for tilapia aquacultural use. The 48‐h acute toxicity (50% lethal concentration, LC50; 48 h) of AZT was determined for Oreochromis niloticus. Thereafter, fish were exposed to 0, 1, 50 and 100 mg L^?1 AZT during 14 days (chronic exposure) and measured the haematological variables, the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione‐S‐transferase (GST) and the concentration of glutathione (GSH), protein carbonyl and lipid peroxidation in the liver; histopathology was analysed the liver, gills and kidneys. The LC50; 48 h was >100 mg L^?1. No fish died during chronic exposure. Haematocrit and haemoglobin concentration increased in fish exposed to 50 and 100 mg L^?1, and the total number of leucocyte and thrombocyte increased after exposure to 100 mg L^?1 AZT, suggesting a stimulation of defence cell production. In the liver, the antioxidant enzyme activities did not change, but GST activity and the GSH level increased in fish exposed to 100 mg L^?1 AZT. Oxidative stress did not occur. Histopathological index (HI_L) indicates moderate liver damage; minor histological changes in the gill and no change in the kidneys. AZT was considered non‐toxic for O. niloticus after acute exposure and, although it causes moderated histopathology in the liver after chronic exposure, this antibiotic may be an alternative against bacterial infections, depending on its efficacy to control bacterial disease in fish.  相似文献   

急性和慢性低氧胁迫对卵形鲳鲹鳃器官的影响   总被引：1，自引：0，他引：1  

陈世喜  王鹏飞  区又君  李加儿  温久福  王雯  谢木娇 《南方水产科学》2017,(1):124-130

文章研究了窒息点以上的低氧胁迫对卵形鲳鲹(Trachinotus ovatus)鱼体鳃器官的影响,在常温条件下对(32.84±3.99)g的卵形鲳鲹进行了急性(0 h、3 h、6 h、12 h、24 h)和慢性(14 d)的低氧胁迫实验,采用常规石蜡切片和H-E染色以及电子显微技术,观察低氧胁迫对卵形鲳鲹鳃器官的影响。结果显示,卵形鲳鲹在急性低氧胁迫下鳃小片上皮肿胀和抬升;慢性低氧胁迫下随着时间延长,鳃小片上皮细胞与鳃小片分离,鳃呼吸表面积增加。结果表明低氧胁迫对卵形鲳鲹鳃器官具有较大影响,而且慢性胁迫的影响大于急性胁迫。  相似文献   

Effect of feed supplementation with kynurenic acid on the morphology of the liver,kidney and gills in rainbow trout (Oncorhynchus mykiss Walbaum, 1792), healthy and experimentally infected with Yersinia ruckeri          下载免费PDF全文

E Kaczorek  J Szarek  M Mikiewicz  E Terech‐Majewska  P Schulz  J Małaczewska  R Wójcik  A K Siwicki 《Journal of fish diseases》2017,40(7):873-884

Kynurenic acid (KYNA) is an endogenous substance produced on the kynurenine pathway which is primarily known for its neuroactive properties. Recently, it has been proven that KYNA is a selective ligand for G protein‐coupled receptor (GPR 35), presented on immunocompetent cells such as T lymphocytes. This opens up new possibilities of its application as an immunostimulating substance in aquaculture. Thus far, no histopathological investigations in fish have been completed to evaluate influence of KYNA supplementation in feed. This study has been undertaken to determine the effect of feed supplementation with KYNA (2.5, 25, 250 mg kg^?1 of feed) for 28 days on the liver, gills and kidney in healthy fish and experimentally infected with Yersinia ruckeri. In a control group were observed a fatty liver, which is natural for this fish species in the autumn and winter season. As the dose of the supplement was increased, the fat liver changed, it decreased or completely disappeared. Additionally, inflammatory changes occurred in all the analysed organs, and their intensification was dose dependent. In the fish experimentally infected, KYNA caused aggravation of the signs in the liver, kidneys and gills, and the effect was dose dependent. The results implicate that KYNA may be a stressor for fish.  相似文献   

Occurrence of Sparicotyle chrysophrii (Monogenea: Polyopisthocotylea) in gilthead sea bream (Sparus aurata L.) from different mariculture systems in Spain     

Ariadna Sitjà‐Bobadilla  Ma José Redondo  Pilar Alvarez‐Pellitero 《Aquaculture Research》2010,41(6):939-944

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不同盐度和驯养时间中华鲟子二代幼鱼鳃的显微结构变化   总被引：1，自引：0，他引：1  

刘娟娟  王京树  赵珣  杨元金 《水生态学杂志》2015,36(1):60-65

采用光学显微镜和组织切片方法,研究了不同盐度和驯养时间中华鲟幼鱼鳃及鳃中泌氯细胞的结构变化特征。试验用鱼为1+龄中华鲟子二代,平均全长50.7 cm,平均体重约441.9 g。试验前在养殖池中暂养1周。试验设2个盐度梯度(10、15)组和1个对照组(淡水)。盐度驯养为将中华鲟子二代从暂养池转入盐度10海水中驯养,分别取驯养1、3、6、9、12、24、48、72、96、120、144、168、192、216、240和264 h的幼鱼各1尾用于试验;将剩下的鱼再转入盐度15海水中驯养,分别取驯养24、48、72 h的幼鱼各1尾用于试验。结果显示,在淡水组中,中华鲟子二代幼鱼鳃丝发达,细胞饱满,鳃小片宽且长。鳃丝上皮的泌氯细胞数量较少,通常靠近鳃小片基部,呈近椭圆状。与淡水组相比,盐度10组的幼鱼鳃丝主干部明显变窄,鳃小片基部区域及鳃小片上泌氯细胞的数量略有增加,胞体变大,且随驯养时间的延长,出现了明显的细胞膜间隙。幼鱼在盐度15组中鳃丝主干明显变窄变短,鳃小片也显著缩短,鳃泌氯细胞数量显著增加,主要分布在鳃小片基部,有明显的分泌腔。中华鲟子二代幼鱼能够快速适应外界水体盐度的变化,这与鳃及鳃上皮中调节渗透压功能细胞的数量和结构变化相适应。  相似文献   

Effect of hydrogen peroxide and/or Flavobacterium psychrophilum on the gills of rainbow trout,Oncorhynchus mykiss (Walbaum)          下载免费PDF全文

M M M Henriksen  P W Kania  K Buchmann  I Dalsgaard 《Journal of fish diseases》2015,38(3):259-270

The immune response and morphological changes in the gills of rainbow trout fry after immersion in hydrogen peroxide (H₂O₂), Flavobacterium psychrophilum or combined exposure were examined. The gills were sampled 4, 48, 125 and 192 h after exposure, and the regulation of expression of the following genes was investigated using qPCR: IgT, IgM, CD8, CD4, MHC I, MHC II, IL-4/13A, TcR-β, IL-10, IL-1β, IL-17, SAA and FoxP3. Bacteria were not observed in haematoxylin-and-eosin-stained gill tissue, but the presence of F. psychrophilum 16S rRNA was detected using qPCR. The 16S rRNA levels were correlated with gene expression. Although pretreatment with H₂O₂ before immersion in F. psychrophilum did not significantly alter the amount of bacteria found in the gill, the immune response was influenced: exposure to F. psychrophilum resulted in a negative correlation with expression of IL-17c1, MHC I and MHC II, while pretreatment with H₂O₂ resulted in a positive correlation with IL-4/13A and IgM. Exposure to either H₂O₂ or F. psychrophilum influenced the regulation of gene expression and damaged tissue. Exposure to both combined altered the immune response to infection and postponed healing of gill tissue.  相似文献   

三唑磷对斑节对虾肝胰腺和鳃的氧化胁迫效应   总被引：1，自引：1，他引：0  

李红艳  张喆  陈海刚  马胜伟  陈炜婷  王涛  蔡文贵 《农业环境科学学报》2013,32(12):2345-2351

采用半静态毒性实验方法测定了三唑磷（Triazophos,OP）对斑节对虾（Penaeusmonodon）的96 h LC50值,同时研究了低（0.3 μg·L－1）、中（0.5 μg·L－1）、高（1 μg·L－1）浓度OP 胁迫1、3、7、14 d和清水恢复7 d后斑节对虾肝胰腺和鳃中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、丙二醛（MDA）含量和总抗氧化能力（T-AOC）的变化趋势。结果表明：在胁迫期间SOD、GPx活性都受到了显著的诱导和抑制,呈一定的变化规律;MDA含量随胁迫时间延长显著升高。肝胰腺T-AOC在低浓度OP胁迫下呈升高-下降-升高的变化趋势,高浓度则显著下降;鳃T-AOC显著升高,而随胁迫时间延长,T-AOC显著降低。经清水恢复7 d后,各浓度组肝胰腺SOD活性仍然显著低于空白组,MDA含量和T-AOC显著高于空白组。低浓度组鳃SOD、T-AOC显著高于空白组,MDA含量和中、高浓度SOD能恢复到正常水平,T-AOC则显著低于空白组。肝胰腺和鳃GPx活性变化情况相同,均为低、中浓度显著升高,高浓度显著下降。上述结果显示,OP 对斑节对虾的抗氧化系统有显著的影响,且具有组织特异性,肝胰腺对OP较为敏感。斑节对虾对一定浓度OP所带来的氧化损伤具有一定的自我修复能力,但在短期内无法完全修复。  相似文献