Menadione Sodium Bisulphite (MSB) enhances the resistance response of tomato,leading to repel mollusc pests          下载免费PDF全文

Estefanía Carrillo‐Perdomo  David Jiménez‐Arias  Ángel Aller  Andrés A Borges 《Pest management science》2016,72(5):950-960

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植物激活蛋白对番茄防御酶活性的影响   总被引：3，自引：0，他引：3  

李丽  刘峥  杨秀芬  邱德文 《湖南农业大学学报(自然科学版)》2008,34(5)

用2 μg/mL植物激活蛋白处理番茄叶片,测定番茄叶片内苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、POD同工酶、多酚氧化酶(PPO)和超氧化物歧化酶(SOD)含量.结果表明:经植物激活蛋白诱导处理后,防御酶活性明显增强.诱导1 d后,PAL活性最大,是对照的3.93倍,8 d后仍为对照的3.4倍;POD和PPO诱导后4 d活性最高,分别比对照增加118.72% 和101.79%,8 d 后仍比对照高;SOD活性诱导后4 d达高峰,8 d后稍高于对照.POD同工酶活性诱导后3 d比对照增加了88.96%,同时还出现了新的酶带.  相似文献   

尿核苷二磷酸糖基转移酶及其抑制子在植物激活剂中研究与应用     

王勇  包兴涛  赵晓珍  薛伟  陈卓 《中国农学通报》2018,34(3):58-69

为了创制以尿核苷二磷酸糖基转移酶(UDP-glucosyltransferases, UGT)为靶标的植物激活剂,本文归纳了UGT的结构特征、反应机理,及其参与水杨酸（Salicylic acid, SA）的代谢机制和UGT调控机制。总结了UGT促进SA信号转导通路及诱导植物产生系统性获得性抗性的机制。以Imprimatins和ILA化合物为例,阐述了UGT抑制剂促进植物SA信号转导通路及诱导SAR活性的机制,指出了基于UGT的植物激活剂的开发策略及应用前景。  相似文献   

PI3K信号通路关键基因在猪卵泡发育中的表达规律     

辛晓萍  袁晓龙  陈炫  张爱玲  陈赞谋  张哲  张豪  李加琪 《华南农业大学学报》2018,39(2):23-28

【目的】研究不同时期猪卵巢卵泡发生的形态特征及磷脂酰肌醇3–激酶(Phosphoinositide 3-kinase,PI3K)信号通路关键基因在卵泡发生发育中的作用。【方法】通过HE染色观察12日龄、30日龄、70日龄、20月龄卵泡期及黄体期、48月龄的长大二元母猪卵巢卵泡发育的形态变化,应用实时荧光定量PCR检测PI3K通路关键基因在这些时期的表达规律,并通过Western blot法检测PI3K通路重要的下游效应因子p-rpS6在不同时期猪卵巢的表达情况。【结果】12日龄母猪卵巢皮质边缘有大量的原始卵泡,皮质与髓质交界处可见少量初级卵泡及个别的次级卵泡;30日龄母猪卵巢次级卵泡数量增加;70日龄出现3级卵泡;20月龄卵泡期、黄体期分别有大量成熟卵泡、大体积的黄体;48月龄较难观察到原始卵泡。PI3K通路抑制因子PTEN、TSC1、TSC2与激活因子PDK1、AKT1、mTOR的mRNA均在12日龄表达量最高,70日龄、48月龄表达量次之,30日龄和20月龄表达量最低;下游效应基因rpS6的mRNA 30日龄表达量最高,20和48月龄表达量最低,p-rpS6蛋白在12日龄、20月龄、48月龄母猪卵巢高表达,在30日龄、70日龄中几乎不表达。【结论】PI3K通路关键基因在不同时期母猪卵巢中的表达水平不同,说明该通路参与了猪卵泡的早期发生及卵泡成熟的调控过程。  相似文献   

Signaling pathways involved in liver injury and regeneration in rabbit hemorrhagic disease, an animal model of virally-induced fulminant hepatic failure     

Rodrigo García-Lastra  Beatriz San-Miguel  Irene Crespo  Francisco Jorquera  Marcelino Alvarez  Javier González-Gallego  María J. Tu?ón 《Veterinary research》2010,41(1)

Management of fulminant hepatic failure (FHF) continues to be one challenging problem, and experimental animal models resembling its clinical conditions are still needed. Rabbit hemorrhagic disease (RHD) fullfils many requirements of an animal model of FHF. This work investigated changes in MAPK, NF-κB, AP-1 and STAT pathways during RHD-induced liver injury. Rabbits were infected with 2 × 10⁴ hemagglutination units of an RHD virus isolate. Apoptosis was documented by the presence of caspase-3 activity and substantial PARP proteolysis at 36 and 48 h postinfection (pi). Infection induced a marked and maintained expression of TNF-α from 12 h pi, while there was only a transitory increase in IL-6 expression. Expression of phosphorylated (p)-JNK, p-p38 and p-ERK1/2 was significantly elevated at 12 h pi. At 48 h pi p-JNK expression was maintained at a maximum level, while that of p-p38 returned to normality and there was no p-ERK1/2 expression. Activation of NF-κB and AP-1 and increased expression of VCAM-1 and COX-2 were observed. No significant changes were detected in activation of STAT1 and STAT3, while SOCS3 expression increased significantly. The current findings suggest that activation of JNK is an essential component in liver injury mediated by the RHD virus and that lack of activation of STAT3, probably mediated by SOCS3 over-expression, would contribute to the inhibition of the regenerative response. Data show the presence of molecular mechanisms contributing to liver damage and the lack of regeneration and they support the usefulness of this model to investigate novel therapeutical modalities in FHF.  相似文献   

High glucose induces vascular smooth muscle cell proliferation and migration through activation of Src/Stat3 signaling pathway     

WANG Jun  QIN Yi-hui 《园艺学报》2017,33(7):1237-1243

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活化剂添加对重金属污染土壤性状的影响     

许学慧  兰亭  刘海云  苗雪梅  刘美英 《干旱区资源与环境》2022,(2):105-109

利用活化剂来活化土壤中的重金属,进而被植物吸收,达到修复重金属污染土壤的目的 ,是一种高效环保的土壤重金属污染修复技术.活化剂的加入一方面提高了修复效果,另一方面也会对土壤性状产生影响.以Cd和Pb污染土壤为研究对象,通过室内盆栽实验研究添加不同浓度(1.0mmol/kg、3.0mmol/kg、5.0mmol/kg)活...  相似文献   

Rosiglitazone inhibits osteoclastogenesis in rheumatoid arthritis by down-regulating RANKL expression and suppressing ERK phosphorylation     

WEI Xiu-ning  ZHENG Dong-hui  MO Ying-qian  MA Jian-da  DAI Lie 《园艺学报》2015,31(5):911-916

AIM: To investigate the effects of rosiglitazone on fibroblast-like synoviocyte (FLS)-induced osteoclastogenesis in rheumatoid arthritis (RA) and the related mechanism. METHODS: RA-FLS were cocultured with peripheral blood monocytes from healthy volunteers in the presence of macrophage colony-stimulating factor (M-CSF) and rosiglitazone. Osteoclasts were assayed by tartrate-resistant acid phosphatase (TRAP) staining. Resorption lacunae area was identified by toluidine blue staining and quantified by image analysis software. The mRNA expression of RANKL and OPG was evaluated by real-time PCR, and the protein levels of RANKL, OPG, p-ERK, p-p38 and p-JNK were measured by Western blot. RESULTS: Compared with control group (without rosiglitazone treatment), rosiglitazone at concentration of 15 μmol/L significantly decreased the number of osteoclasts (P<0.01) and resorption lacunae area (P<0.05). The expression of RANKL at mRNA and protein levels was significantly down-regulated by rosiglitazone at concentration of 15 μmol/L, while the mRNA and protein expression of OPG was up-regulated (P<0.01). Rosiglitazone (15 μmol/L) significantly decreased the protein level of p-ERK (P<0.05), but not the protein level of p-p38 or p-JNK (P>0.05). CONCLUSION: Rosiglitazone inhibits RA-FLS-induced osteoclast formation and its resorption activity by down-regulating RANKL expression and ERK phosphorylation, suggesting that rosiglitazone may inhibit RA osteoclastogenesis and bone resorption.  相似文献   

新型激活蛋白对棉花纤维品质的影响初探     

李建华  龚华春 《江西棉花》2008,30(2):11-16

采用浓度为3μg/ml的新型激活蛋白进行浸种,以及大田不同生育时期喷施,来观察棉花纤维品质指标的变化。结果表明,新型激活蛋白对于提高棉花纤维长度、整齐度、比强度、伸长率、反射率、纺纱均匀性指数等具有显著的效果,并能有效地降低纤维麦克隆值和黄度。通过灰色关联度分析,对本试验涉及的棉花纤维品质8个指标进行重要性排序为:长度>麦克隆值>整齐度>比强度>反射率>纺纱均匀性指数>伸长率>黄度。按照各指标的重要性不同,各处理效果依次为:浸种 幼苗期喷雾>浸种 现蕾期喷雾>浸种>浸种 初花期喷雾>初花期喷雾>对照,其中以浸种、浸种 幼苗期喷雾和浸种 现蕾期喷雾处理优势最强,可以作为激活蛋白应用于棉花大田生产的有效措施。  相似文献   

结肠癌组织中中期因子、尿激酶型纤溶酶原激活物的表达及意义     

朱涛  曹虹  屈淑平 《湛江医学院学报》2012,30(2):126-128

目的了解结肠癌组织中中期因子（MK）、尿激酶型纤溶酶原激活物（uPA）表达及其与结肠癌临床病理特征的关系。方法收集55例病理学确证的结肠癌组织标本（病例组）和30例结肠癌癌旁的正常组织标本（对照组）,采用免疫组织化学方法（SP法）检测其MK、uPA的表达。结果病例组中MK、uPA表达率分别为56.36%、61.82%,对照组分别为13.33%、16.67%,两组的差异均有统计学意义（P〈0.01）。结肠癌组织中MK、uPA表达与癌组织的分化程度、浸润深度、淋巴结转移有相关性,而与性别和年龄无相关性。MK与uPA表达呈正相关（r=0.44,P〈0.05）。结论 MK、uPA表达与结肠癌侵袭、转移密切相关,二者联合检测可作为判断结肠癌发展及预后的指标。  相似文献