油菜素内酯对香蕉不定芽增殖的影响     

郑晓丹  丰锋 《福建果树》2014,(1):1-8

以香蕉不定芽为材料,MS+6-BA 4.0 mg·L-1+NAA 0.2 mg·L-1为基本培养基,采用单因素试验设计方法,研究油菜素内酯对香蕉不定芽增殖及酶活性变化的影响。结果表明,随着香蕉不定芽的形成,不加油菜素内酯,SOD活性逐渐下降,然后急剧上升;POD活性先上升,然后不断下降。油菜素内酯处理下SOD活性总体呈下降-上升-下降的变化规律,POD活性呈下降-上升-下降的规律。培养基中加入0.3 mg·L-1油菜素内酯的香蕉不定芽中的可溶性糖(3.73 mg·g-1)、脯氨酸含量(67.10μg·g-1)和株高(5.00 cm)都显著高于其他处理。  相似文献   

中国对虾亲虾生殖周期中消化酶活性及组织生化成分的变化   总被引：5，自引：0，他引：5       下载免费PDF全文

吴垠 《水产学报》2003,27(6):550-557

叙述了中国对虾亲虾(海捕亲虾与池养亲虾)在生殖周期中肝胰腺、中肠的消化酶活性及卵巢、肝胰腺、血淋巴生化成分的研究结果。蛋白酶、脂肪酶在卵巢成熟的Ⅳ期前活性较高，临产卵时(Ⅴ期)活性明显下降，而且海捕亲虾与池养亲虾具有相同的变化趋势；中国对虾卵巢、肝胰腺和血淋巴的蛋白质和脂肪含量随性腺发育有不同的变化规律，其中总蛋自在这几种组织中均随性腺发育含量增加，至Ⅳ期(肝胰腺)和Ⅴ期(卵巢、血淋巴)达到高峰值；卵巢的总脂和甘油三酯从发育Ⅰ期-Ⅴ期随着性腺指数的增长而有规律的上升，但肝胰腺脂肪含量在卵巢发育初期(Ⅰ期-Ⅱ期)未见明显变化，Ⅱ期以后，肝胰腺脂肪含量逐渐下降，当卵巢脂肪含量达到最高值时，肝胰腺脂肪含量正好下降到最低点。同时，还对中国对虾卵巢发育过程中蛋白质和脂肪的积累问题进行了分析和讨论。  相似文献   

An ex vivo evaluation of efficacy of refrigerated canine plasma     

Adam R. Grochowsky DVM  Elizabeth A. Rozanski DVM  DACVECC  DACVIM  Armelle M. de Laforcade DVM  DACVECC  Claire R. Sharp BSc  BVMS  MS  DACVECC  Dawn M. Meola BS  Jessica J. Schavone BS  CVT  Majory B. Brooks DVM  DACVIM 《Journal of Veterinary Emergency and Critical Care》2014,24(4):388-397

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复方中药体外抗猪衣原体活性研究   总被引：1，自引：0，他引：1       下载免费PDF全文

王金福  张永霞  顾剑新  王彤光  孙维平  曹彩琴 《家畜生态学报》2014,35(8):70-71

应用微量Vero细胞培养法,检测复方中药的体外抗衣原体活性,为防治猪衣原体病提供依据。结果表明,复方中药的MIC值是39.06mg/mL,随着中药浓度升高,包涵体的体积缩小、数量减少,直至消失,对照组未见中药对Vero细胞有毒性作用。试验结果显示,复方中药体外对猪衣原体有抗菌活性。  相似文献   

Effects of different sources of carbohydrates on intake,digestibility, chewing,and performance of Holstein dairy cows     

Simin Poorkasegaran  Asadollah Teimouri Yansari 《畜牧与生物技术杂志(英文版)》2014,5(1):6

To investigate the effects of different sources of carbohydrates on intake, digestibility, chewing, and performance, nine lactating Holstein dairy cows (day in milk= 100±21 d; body weight=645.7 ± 26.5 kg) were allotted to a 3 × 3 Latin square design at three 23-d periods. The three treatments included 34.91% (B), 18.87% (BC), and 18.86% (BB) barley that in treatment B was partially replaced with only corn or corn plus beet pulp in treatments BC and BB, respectively. The concentration of starch and neutral detergent soluble carbohydrate varied (22.2, 20.2, and 14.5; 13.6, 15.9, and 20.1% of DM in treatments B, BC, and BB, respectively). Cows in treatment BB showed a higher DMI and improved digestibility of DM, NDF, and EE compared with treatments B or BC. Ruminal pH was higher in cows fed on BB (6.83) compared with those that received B or BC treatments (6.62 and 6.73, respectively). A lower proportion of propionate accompanied the higher pH in the BB group; however, a greater proportion of acetate and acetate: propionate ratio was observed compared with cows fed either on the B or BC diet. Moreover, cows fed on the BB diet showed the lowest ruminal passage rate and longest ruminal and total retention time. Eating time did not differ among treatments, rumination time was greater among cows fed on the BB diet compared with the others, whereas total chewing activity was greater than those fed on BC, but similar to those fed on B. The treatments showed no effect on milk yield. Partially replacing barley with corn or beet pulp resulted in an increase in milk fat and a lower protein concentration. Changing dietary NFC with that of a different degradability thus altered intake, chewing activity, ruminal environment, retention time or passage rate, and lactation performance. The results of this study showed that beet pulp with a higher NDF and a detergent-soluble carbohydrate or pectin established a more consistent ruminal mat than barley and corn, thus resulting in higher mean retention time and chewing activity, whereas no changes in 3.5% FCM and milk fat were observed.  相似文献   

Clonal Spread of Salmonella enterica Serovar Infantis in Serbia: Acquisition of Mutations in the Topoisomerase Genes gyrA and parC Leads to Increased Resistance to Fluoroquinolones     

M. Velhner  G. Kozoderović  E. Grego  N. Galić  I. Stojanov  Z. Jelesić  C. Kehrenberg 《Zoonoses and public health》2014,61(5):364-370

Quinolone‐resistant Salmonella Infantis (n = 64) isolated from human stool samples, food and poultry during the years 2006–2011 were analysed for their resistance phenotypes, macrorestriction patterns and molecular mechanisms of decreased susceptibility to fluoroquinolones. Minimum inhibitory concentrations (MICs) of nalidixic acid (NAL) and ciprofloxacin (CIP) were determined by the agar dilution procedure, and the susceptibility to additional antimicrobial agents was determined by the disc diffusion method. To assess the influence of enhanced efflux activity, MICs were determined in the presence and absence of the inhibitor PAβN. The results of pulsed‐field gel electrophoresis (PFGE) typing revealed that quinolone‐resistant S. Infantis in Serbia had similar or indistinguishable PFGE profiles, suggesting a clonal spread. All S. Infantis showed combined resistance to NAL and tetracycline, whereas multiple drug resistance to three or more antibiotic classes was rare (2 isolates of human origin). The MICs ranged between 512 and 1024 μg/mL for NAL and 0.125–2 μg/mL for CIP. A single‐point mutation in the gene gyrA leading to a Ser83→Tyr exchange was detected in all isolates, and a second exchange (Ser80→Arg) in the gene parC was only present in eight S. Infantis isolates exhibiting slightly higher MICs of CIP (2 μg/mL). The inhibitor PAβN decreased the MIC values of CIP by two dilution steps and of NAL by at minimum 3–6 dilution steps, indicating that enhanced efflux plays an important role in quinolone resistance in these isolates. The plasmid‐mediated genes qnr, aac(6′)‐lb‐cr and qepA were not detected by PCR assays.  相似文献   

Prevalence,Serovars and Antimicrobial Susceptibility of Salmonella spp. from Wild and Domestic Green Iguanas (Iguana iguana) in Grenada,West Indies     

W. R. B. Sylvester  V. Amadi  R. Pinckney  C. N. L. Macpherson  J. S. McKibben  R. Bruhl‐Day  R. Johnson  H. Hariharan 《Zoonoses and public health》2014,61(6):436-441

Cloacal swabs from 62 green iguanas (Iguana iguana), including 47 wild and 15 domestic ones from five parishes of Grenada, were sampled during a 4‐month period of January to April 2013 and examined by enrichment and selective culture for the presence of Salmonella spp. Fifty‐five per cent of the animals were positive, and eight serovars of Salmonella were isolated. The most common serovar was Rubislaw (58.8%), a serovar found recently in many cane toads in Grenada, followed by Oranienburg (14.7%), a serovar that has been causing serious human disease outbreaks in Japan. Serovar IV:48:g,z₅₁:‐ (formerly, S. Marina) highly invasive and known for serious infections in children in the United States, constituted 11.8% of the isolates, all of them being from domestic green iguanas. Salmonella Newport, a serovar recently found in a blue land crab in Grenada, comprised 11.8% of the isolates from the green iguanas. The remaining four less frequent serovars included S. Javiana and S. Glostrup. Antimicrobial susceptibility tests conducted by a disc diffusion method against amoxicillin–clavulanic acid, ampicillin, cefotaxime, ceftazidime, ciprofloxacin, enrofloxacin, gentamicin, nalidixic acid, streptomycin, tetracycline and trimethoprim–sulfamethoxazole showed that drug resistance is minimal, with intermediate susceptibility, mainly to streptomycin, tetracycline and cefotaxime. This is the first report of isolation and antimicrobial susceptibilities of various Salmonella serovars from wild and domestic green iguanas in Grenada, West Indies.  相似文献   

鳜嗜水气单胞菌GYK1株胞外产物提取、纯化及生物学活性分析   总被引：2，自引：0，他引：2  

潘厚军  石存斌  刘瑞明  胡学峰  张敏  吴淑勤 《南方水产》2006,2(3):1-6

以鳜分离的嗜水气单胞菌GYK1株为代表,采用玻璃纸覆盖技术提取了8株嗜水气单胞菌和1株温和气单胞菌的胞外产物,SDS-PAGE电泳分析显示,8株嗜水气单胞菌的胞外产物均有35kDa的共同蛋白带。酶活性和溶血性分析表明,GYK1株胞外产物具有酪蛋白酶、淀粉酶、脂肪酶、明胶酶活性,但不具备脲酶活性;溶血性较强,蛋白浓度为365μg·mL-1的胞外产物对鳜血细胞的溶血价为213,对加州鲈、银鲫、小白鼠、兔、绵羊、人O型血的红细胞的溶血价在211~214之间。应用聚丙烯酰胺葡聚糖凝胶柱层析,对GYK1株的胞外产物进行了初步纯化,得到分子量为35kDa的蛋白带,该纯化产物具溶血性,但溶血性比粗胞外产物降低,对鳜和小鼠血细胞的溶血价均为25。  相似文献   

溶藻胶弧菌脂多糖对大菱鲆白细胞吞噬活性的影响   总被引：1，自引：0，他引：1  

叶海斌  朱安成张涛 李天保 《齐鲁渔业》2006,23(3):34-35

针对溶藻胶弧菌脂多糖在增强大菱鲆非特异性免疫功能、提高机体抗病能力方面的作用机理,开展了研究工作。实验中以浓度为2．4mg／ml 溶藻胶弧菌脂多糖,通过口服和注射的方式接种平均体重47．2g 大菱鲆幼鱼,接种后第7、15、 30天,分别采血根据血细胞吞噬作用检测结果计算吞噬百分比和吞噬指数。结果显示,溶藻胶弧菌脂多糖对白细胞吞噬活性有明显促进作用,在增强白细胞的吞噬能力方面作用不显著。  相似文献   

Assessment of the physiological effect of altered salinity on greenlip (Haliotis laevigata) and blacklip (Haliotis rubra) abalone using respirometry     

Steve Edwards 《Aquaculture Research》2003,34(14):1361-1365

The early physiological response (3 days) of greenlip (Haliotis laevigata Leach) and blacklip (H. rubra Leach) abalone to a single abrupt change in salinity was monitored using a multi‐channel open‐circuit respirometer. The range of salinity tolerance for both species in this trial was 25–40 ppt. Indications from other trials were that a margin of 2 ppt outside this range will cause mortality. Serum volume was increased by reduced salinity as much as 25% in the short term, with equilibration of the concomitant whole body weight increase (9.2±0.5%) occurring within 1 h. The serum volume appeared to be decreased at high salinity. There was little underlying change in basal oxygen usage levels, but significant behavioural changes that affected overall oxygen consumption. Both high and low salinity appeared to reduce activity. Animals in low salinity exhibit partial recovery of activity levels after 1 day in a manner similar to other stress responses. Animals at high salinity (40 ppt) did not show recovery of activity levels over 3 days. Overall, these results suggest that greenlip and blacklip abalone will have little trouble tolerating moderately low‐salinity environments.  相似文献