Undifferentiated tumor in the ovary of a corn snake (Elaphe guttata guttata)     

Petterino C  Bedin M  Podestá G  Ratto A 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):95-100

A 6-year-old intact female corn snake (Elaphe guttata guttata) was presented with a 3-week history of anorexia. Coelomic radiographs revealed a 9 x 4 cm soft tissue opacity suggestive of a right ovarian enlargement. The mass (9 x 5 x 4 cm) was surgically removed, and multiple smears from tissue sections were stained with Diff-Quik. Multiple tissue samples from the mass were collected and fixed in formalin. Cytologic specimens were moderately cellular and contained light pink amorphous background material. The cells were primarily spindle-shaped with moderate to marked anisocytosis and anisokaryosis. Cells sometimes were round to polygonal, and rarely were arranged in small clusters. Macrophages occasionally were observed. Histologic specimens consisted of a highly cellular mass composed of pleomorphic, spindle-shaped cells and, occasionally, round to polygonal cells arranged in irregular fascicles. The neoplastic cells were immunoreactive for cytokeratin (AE1/AE3), smooth muscle actin, and skeletal muscle actin, but did not stain for vimentin or desmin. On the basis of the morphologic and immunohistochemical results, a diagnosis of ovarian undifferentiated carcinoma was made. In this report, we describe the challenges of using immunohistochemistry to diagnose this uncommon type of tumor in reptiles.  相似文献   

Cerebrospinal fluid from a 6-year-old dog with severe neck pain     

Bauer NB  Bassett H  O'Neill EJ  Acke E 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):123-125

A 6-year-old, intact female, Labrador Retriever/Terrier cross was presented to the University Veterinary Hospital, University College Dublin with a 3-week history of therapy-resistant cervical pain and intermittent fever. Physical examination findings included marked cervical pain resulting in neck extension and vocalization. Examination of the CSF revealed mild pleocytosis (total nucleated cells = 0.009 x 10(9)/L, reference interval <0.005 x 10(9)/L). Cytocentrifuged preparations of the CSF were of low cellularity, containing predominantly macrophages and occasional small lymphocytes. Several small- to medium-sized fragments of a slightly granular, amorphous, eosinophilic substance were observed. The majority of mononuclear cells were located within this material, in small groups of 3-13 cells. The amorphous foamy material stained positive with Luxol fast blue, suggestive of myelin-like material. The dog was euthanized and postmortem examination revealed intervertebral disk protrusion between C2 and C3. Hematoxylin- and Luxol fast blue-stained histopathologic sections of brain and spinal cord revealed only mild hemorrhage. The extracellular material in the CSF of this dog may have been caused by myelin degeneration or leakage of phospholipids from damaged cells. Because no histologic evidence of demyelination was observed with the disk extrusion, the myelin-like material in this case was thought to be the product of phospholipid breakdown from damaged cellular membranes. Three cases of dogs with spinal cord disease and myelin-like material in the CSF have been reported previously. The clinical significance of this finding is still unknown.  相似文献   

Squash-prep cytology in the diagnosis of canine and feline nervous system lesions: a study of 42 cases     

De Lorenzi D  Mandara MT  Tranquillo M  Baroni M  Gasparinetti N  Gandini G  Masserdotti C  Bonfanti U  Bertolini G  Vian P  Bernardini M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(2):208-214

BACKGROUND: The increased sophistication of imaging techniques in veterinary medicine allows the detection of a wide variety of intracranial and intraspinal lesions; however, imaging often does not provide a definitive diagnosis for nervous system (NS) lesions. Cytology is emerging as a useful diagnostic tool for obtaining a fast and accurate assessment of NS lesions, but little information is available for dogs and cats. OBJECTIVES: The purpose of this study was to assess the accuracy of cytologic evaluation of squash samples from NS lesions in dogs and cats and to consider cytology-based diagnostic guidelines and sources of misdiagnosis. METHODS: Cytologic specimens from masses localized in the central and peripheral NS taken during surgery or postmortem examination were classified into 3 groups according to the final histopathologic diagnosis: Group 1 = completely correct diagnosis, when the cytologic diagnosis and final histologic diagnosis were exactly correlated; Group 2 = partial correlation, when the cytologic diagnosis only partially correlated with the final histologic diagnosis, and Group 3 = no correlation, when the cytologic diagnosis was incorrect and there was no correlation with the general histologic type of lesion. The diagnostic accuracy of cytopathology was calculated by considering the histopathologic diagnosis as the "gold standard," and calculating a 95% confidence interval (CI). RESULTS: A total of 42 animals (33 dogs and 9 cats) were included in the study. The cytologic diagnoses were classified in Group 1 for 32 cases (76%; 95% CI 0.63-0.89), in Group 2 for 6 cases (14%; 95% CI 0.04-0.25), and in Group 3 for 4 cases (10%; 95% CI 0.006-0.18). Considering both complete and partial correlation as an adequate result, cytologic diagnosis was satisfactory in 90% of biopsies. CONCLUSIONS: Although the current series of cases is relatively small, cytologic evaluation of squash preparations can be considered a fairly accurate and reliable tool in the diagnosis of NS lesions.  相似文献   

Bold fusion: transtracheal wash from a Jersey calf     

Brazzell JL  Kaese HJ  Borjesson DL 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(2):247-249

A 10-week-old, female, Jersey calf was referred to the University of Minnesota Veterinary Medical Center for evaluation of difficult breathing and inappetence of 18-hours duration. Cytologic examination of a transtracheal wash specimen revealed pyogranulomatous inflammation, with increased numbers of multinucleated cells. Differential diagnoses included established bacterial infection, viral infection, fungal infection, and foreign body reaction. Immunocytochemical staining was done on a direct smear of the transtracheal wash using a cocktail of 4 mouse monoclonal antibodies against 3 human respiratory syncytial virus proteins. Strong intracytoplasmic staining within mononuclear cells was observed and a diagnosis of bovine respiratory syncytial virus infection was made. Immunocytochemistry may be used as a rapid, inexpensive, adjunctive diagnostic tool for the antemortem diagnosis of bovine respiratory syncytial virus on transtracheal wash specimens.  相似文献   

Evaluation of static telepathology in veterinary diagnostic cytology     

Maiolino P  Restucci B  Papparella S  De Vico G 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(3):303-306

BACKGROUND: Telepathology is the process of diagnostic pathology performed on digital images viewed on a display screen rather than by conventional light microscopy with glass slides. At present, electronic mail (e-mail) attachment is the most common communication medium for telepathology, because it is relatively simple and incurs minimal cost. OBJECTIVE: Our objective was to test whether telediagnosis through Internet e-mail could be applied to veterinary diagnostic cytology. METHODS: Using 20 cytology cases, on which a consensus diagnosis was reached by 3 experienced pathologists, a total of 130 images were digitized and sent as e-mail attachments from the Unit of Anatomic Pathology of the Faculty of Veterinary Medicine of Naples to the Unit of Pathology of the Faculty of Veterinary Medicine of Messina, Italy. The images were reviewed at the Unit of Pathology of Messina, where the consulting pathologist formulated his diagnoses by visualizing the images on the monitor of a computer. The telediagnoses were compared with the consensus diagnoses and with the consulting pathologist's interpretation using conventional light microscopy at a later date. RESULTS: The median time to capture images was approximately 30 minutes for each case. The median time to make a telecytodiagnosis was a few seconds for each case. Overall, there was good agreement (85%) between the consensus diagnosis and the consultant's telediagnosis. In 100% of the cases there was agreement between the consulting pathologist's telediagnosis and conventional glass slide diagnosis. CONCLUSION: Telepathology by e-mail provides acceptable efficacy and a faster turnaround time than post and can be applied to veterinary diagnostic cytology.  相似文献   

Gallbladder aspirate from a dog     

Neel JA  Tarigo J  Grindem CB 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(4):467-470

A 7-year-old, male, castrated, Labrador Retriever with a history of pancreatitis and inflammatory bowel disease presented for vomiting and anorexia. Serum biochemistry findings were indicative of cholestasis, hepatocellular insult, and decreased hepatic function. Ultrasound examination showed sediment and gas within the gallbladder, and a diagnosis of emphysematous cholecystitis was made. Emergency gallbladder resection was performed. Cytologic examination of bile fluid collected at surgery showed a mixed population of bacteria (bactibilia) together with fungal organisms consistent with Cyniclomyces guttulatus (previously known as Saccharomycopsis guttulatus). Similar fungal organisms were seen on a fecal smear. Bacteria cultured were normal gastrointestinal flora, supporting ascending infection; the fungal organisms were interpreted as incidental. Histopathology of the gallbladder indicated active (suppurative) and chronic (lymphocytic) cholecystitis and sections of liver tissue had evidence of chronic liver disease. A positive liver culture indicated concurrent bacterial hepatitis or cholangiohepatitis. Despite supportive care, the dog continued to decline and was euthanized 30 days later. Necropsy results confirmed end stage liver disease, but an initiating cause was not found. This case highlights the role of bactibilia in the development of acute cholecystitis and the unique cytologic appearance of C guttulatus as an incidental finding in bile fluid.  相似文献   

大白菜Ogura雄性不育系及保持系花药发育的细胞学观察   总被引：1，自引：0，他引：1  

王书强  刘俊英  崔辉梅 《新疆农业科学》2011,48(6):996-1000

[目的]研究3个大白菜Ogura雄性不育系及保持系花药小抱子发育过程.[方法]对3对大白菜Ogura雄性不育系及其保持系花药发育进行细胞学观察.[结果]3个大白菜Ogura雄性不育系(OBY,OBK,OD5)的小孢子败育均发生在单核早期,在开花前完全败育.[结论]不育系绒毡层液泡化早于保持系,花粉母细胞减数分裂时不育系绒毡层细胞已开始退化,而保持系(BY,BK,D5)绒毡层自然解体,供给小孢子发育所需的营养物质和发育空间.  相似文献   

植物多倍体的形成及其二倍化机制     

田恩堂  贺朱林  周平  杨梅  王天娅  孟金陵 《湖北农业科学》2017,56(11)

大约70%的开花植物在进化史上都经历过多倍化的过程,多倍体植物通常具有较强的可塑性,容易形成新物种。而新形成的多倍体往往不能稳定存在,其基因组需要再经历一次二倍化的过程,从而在基因水平上和细胞学水平上更接近二倍体。对多倍体二倍化的机制和相关研究的未来发展情况进行了探讨。  相似文献   

What is your diagnosis? Digit aspirate from a dog          下载免费PDF全文

Melinda S. Camus  Annabelle Burnum  Elizabeth W. Howerth  Christopher J. Brouwer  Ajay Sharma  Chien‐tsun Huang 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(4):635-636

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What is your diagnosis? Skin scraping from a hellbender          下载免费PDF全文

Allison Dusick  Bente Flatland  Linden Craig  Sylvia Ferguson 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(1):183-184

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