不同型号酵母浸粉及补糖对异养微藻生长的影响   总被引：1，自引：0，他引：1  

郑世文  赵玉峰  毕生雷 《现代农业科技》2014,(8)

微藻在FM802、FM902和FM760 3种不同型号的安琪酵母浸粉配制的培养基中进行异养生长,培养条件为28℃、200 r/min的摇床中进行暗培养,培养过程中补糖1次,每隔24 h取样20 mL进行分析检测。结果表明:补糖情况下,型号为FM802的酵母浸粉比FM902培养微藻的效果稍好,FM760培养效果稍差;补糖有利于微藻干物质重的增加;在pH值为4.2~5.5的培养基中,微藻仍能快速生长。  相似文献   

大兴安岭主要树种生长过程表中有关调查因子的补充计算及其应用     

牟亚男  曹长春等 《内蒙古林业调查设计》2000,23(3):21-24

在使用林分生长过程表中 ,文章就林分株数、立木材积、平均生长量、生长率、胸高形数、稀疏株数等因子的补充计算及其应用方面作初步探讨  相似文献   

Low-protein supplement increases protein retention and reduces the amounts of nitrogen and phosphorus wasted by rainbow trout fed on low-fat herring     

RUOHONEN  VIELMA  & GROVE 《Aquaculture Nutrition》1999,5(2):83-91

The growth, food utilization and amounts of nitrogen and phosphorus lost into the water by rainbow trout (initial size 485 g) fed on low-fat Baltic herring and supplemented with a low-protein dry diet fed separately were studied in an 18-week net cage trial. Similarly to balancing protein and nonprotein energy in full diets, giving nonprotein energy in different feeds several hours apart improved the protein retention of low-fat herring to a level comparable to a commercially available dry diet control. The amounts of nitrogen and phosphorus lost into the water were also reduced to the same extent. The losses from the plain herring-feeding were twice as high. The improved protein retention and reduced nutrient losses were accompanied by 15–25% reductions in growth of the fish compared with the control dry diet. The growth of the fish fed on plain herring was 14% lower than the growth on the dry diet control.  相似文献   

In vitro somatic embryogenesis in turf-type bermudagrass: roles of abscisic acid and gibberellic acid, and occurrence of secondary somatic embryogenesis   总被引：2，自引：0，他引：2  

L. Li  R. Qu 《Plant Breeding》2002,121(2):155-158

Bermudagrass is an important warm‐season turfgrass species that is recalcitrant in regeneration in tissue culture. In a previous report, we observed that somatic embryogenesis of immature inflorescence culture was substantially improved when low levels of 2,4‐dichloro‐phenoxy acetic acid (1 mg/l) and 6‐benzylaminopurine (BAP, 0.01 mg/l) were included in the callus induction medium. The object of this study was to further improve the culture conditions to enhance somatic embryo formation and plantlet regeneration. It was shown that the abscisic acid supplement (2 or 5 mg/l) to the above callus induction medium further enhanced somatic embryogenesis in hybrid bermudagrass (Cynodon dactylon×Cynodon transvaalensis) cv. ‘Tifgreen’. The addition of gibberellic acid (0. 2 mg/l) to the BAP (1 mg/l)‐containing regeneration medium accelerated germination/regeneration of the somatic embryos. Secondary and repetitive somatic embryogenesis, which is rarely reported in monocots, was observed in common bermudagrass (Cynodon dactylon, cv. ‘Savannah’), and a full course of such a development was captured by a periodical microphotography. Scanning electron microscopy further confirmed the observation.  相似文献   

福建大洋人工草地放牧羊补饲矿物质添加剂效果观察     

邵良平  谢永乐  林藩平  李建生  黄小红 《福建农林大学学报(自然科学版)》1993,(4)

根据大洋人工草地牧草的矿物质元素的含量和放牧羊矿物质元素需要量配制含有8种元素的矿物质添加剂,对放牧的罗姆尼羊和四川麻羊进行补饲试验,结果表明,2种羊的平均日增重、Hb、TSP和血清中Co、P元素的含量试验组都显著高于对照组(P<0.05),四川麻羊全血GSH-PX试验组显著高于对照组,罗姆尼羊血清中Mn、K元素的含量试验组显著高于对照组(p<0.05),2种羊的RBC、PCV、血糖、CP、GSH-PX(罗姆尼羊),以及血液中Sc、Ca、Na、Mg,Mn(四川麻羊)试验组均高于对照组,但差异都不显著,(p>0.05)。  相似文献   

Preference of Sheep for Three Forms of Mucuna Forage and the Effect of Supplementation with Mucuna Forage on the Performance of Sheep     

Adjorlolo LK  Amaning-Kwarteng K  Fianu FK 《Tropical animal health and production》2004,36(2):145-156

Assessment of the preference of sheep for fresh, dried or ensiled forms of mucuna (Mucuna pruriens var utilis) forage was followed by investigations into the effect of supplementing straw-based diets with the forage. Four sheep were offered fresh, dried and ensiled forms of the forage in a cafeteria style to assess their preference. In the second experiment, 20 sheep were randomly assigned to four dietary treatments, namely, alkali-treated straw only (M0), treated straw supplemented with mucuna forage at 0.3% (M10), 0.6% (M20) or 0.75% (M25) of body weight (approximately 10%, 20% and 25%, respectively, of the total dry matter intake). The daily feed intakes were determined and the sheep were weighed weekly for 10 weeks. The sheep showed a marked preference for fresh mucuna forage over either the dried or ensiled forms. The total dry matter intake increased by 15% and 21%, respectively, with M20 and M25. All the groups lost weight over the feeding period. However, only M0 gave weight losses during the second half of the feeding period. The feed conversion efficiency followed a trend similar to that for weight gains. M20 had the greatest effect on growth and feed conversion efficiency.  相似文献   

HPLC法检测保健食品中大蒜素的含量   总被引：2，自引：0，他引：2  

徐丽红  王建清  张巧艳  李晓敏  邱承海  袁利文 《浙江农业学报》2012,24(4):711-717

选取固体类、液体类、半固体类3类保健食品为研究对象,考察了提取溶剂、乙醇体积分数、提取温度、色谱柱对检测结果的影响,建立了高效液相色谱法测定保健食品中大蒜素主要功效成分二烯丙基三硫醚(diallyl trisulfide,DATS)、二烯丙基二硫醚(diallyl disulfide,DADS)的检测方法。试样以无水乙醇超声提取,经C18柱(250 mm×4.6 mm,5μm)分离;流动相为V(乙腈)∶V(水)=68∶32;流速1.0 mL.min-1;紫外检测波长240 nm;检出限DATS 2.0 mg.kg-1,DADS 1.5 mg.kg-1。大蒜素浓度在6.26～6 262.24 mg.kg-1范围内呈良好线性关系(r=0.9999),分别对固体类、液体类、半固体类样品进行高低两水平添标回收试验,平均回收率DATS为97.6%～105.8%,DADS的回收率为95.3%～105.4%。重复性变异系数1.5%～2.7%,再现性变异系数3.8%～5.6%。同时将HPLC与GC分析相比较,结果无显著性差异。该方法简便快速,准确可靠,适用于多种保健食品中大蒜素含量的检测。  相似文献   

荒漠草原家庭牧场不同放牧管理模式下草畜能量平衡模拟研究     

刘通  李治国  韩国栋  赵萌莉  何晓蕾  王静 《内蒙古草业》2013,(2):14-18

本研究以内蒙古乌兰察布市四子王旗家庭牧场调研数据为基础,利用ACIAR草畜平衡模型对荒漠草原家庭牧场在全年放牧和季节牧场及补饲措施的家畜需求与草地供给之间的能量平衡关系进行模拟研究,目的是了解家庭牧场的草畜平衡状态,为草地畜牧业管理提供理论依据。结果表明,划分季节牧场利用可增加家畜在冬季的实际能量摄入,减少在夏季的能量摄入;补饲可以缓解在家畜关键生长期对于能量的需求,并在不额外增加补饲量的条件下,划分季节牧场的家畜也表现出冬季实际能量摄入增加,夏季能量摄入减少。  相似文献   

A randomised,blinded, crossover study to assess the efficacy of a feed supplement in alleviating the clinical signs of headshaking in 32 horses     

W. A. Talbot  G. L. Pinchbeck  D. C. Knottenbelt  H. Graham  S. A. Mckane 《Equine veterinary journal》2013,45(3):293-297

Reasons for performing study: Feed supplements are commonly used by owners to alleviate headshaking; however, randomised, controlled trials are required to assess their efficacy. Objective: To determine the efficacy of a feed supplement for alleviation of the clinical signs of headshaking using a randomised, blinded, placebo‐controlled trial. Methods: Using a crossover design, 44 horses previously diagnosed with chronic idiopathic headshaking received both the supplement and a matching placebo per os for 28 days with a washout period between of 14 days. Video recordings were taken at rest and exercise prior to the study and at the end of both periods of treatment. The degree of headshaking was assessed in a blinded, randomised manner by 2 veterinary surgeons. At the same time points, owners completed a questionnaire to assess the severity of headshaking signs. A Wilcoxon signed rank test was used to compare the scores while on supplement and placebo. Results: Using the video assessments, there was no significant difference between scores while on supplement compared with placebo (P = 0.7). Using the questionnaire responses, there was no significant difference between scores for any activity when the placebo and the supplement were compared with each other. However, owners reported significant improvement during all activities for both placebo and supplement compared with pretreatment scores. Conclusions and potential relevance: The supplement offered no benefit over a placebo in alleviating the clinical signs of headshaking. There appeared to be a significant proxy placebo effect when the outcome was based on subjective owner perception of clinical signs. This study demonstrated no beneficial effect of this supplement on the clinical signs of headshaking. The study did show a significant placebo effect, thereby highlighting the necessity of properly conducted, randomised controlled trials, with blinding, to assess true treatment effects in trials in animals.  相似文献   

The impact of highly concentrated Mo and Cu dietary supplements,fed as a bolus,on the efficacy of chelated versus inorganic Cu in cattle on a low-Cu diet     

ND Grace  DM West  SL Smith 《New Zealand veterinary journal》2013,61(6):345-348

AIM: To compare the efficacy of chelated versus inorganic forms of dietary Cu supplements, fed as a bolus, when challenged by a daily bolus of dietary Mo in cattle on a low-Cu diet.

METHODS: Forty non-lactating, Friesian dairy cows of adequate Cu status were assigned to four groups and fed a basal diet of baled silage containing 5.3 mg Cu and 0.4 mg Mo/kg DM. The experimental design was a factorial of two chemical forms of supplemental Cu and two levels of Mo intake, provided as pelleted grain supplements made from crushed barley/molasses plus Cu and Mo. The supplements contained 140 mg Cu/kg as Cu sulphate pentahydrate (CS), 140 mg Cu/kg as Cu glycinate (CG), CS plus 38 mg Mo/kg as sodium molybdate (CS+Mo), or CG plus 38 mg Mo/kg (CG+Mo). Commencing on Day 0, supplements were fed once daily (offered 1–1.2 kg/cow) and were completely consumed within 5–10 minutes, which constitutes a bolus type of administration. Liver samples were collected by biopsy at Days ?24, 13, 41 or 47, and 69 for Cu determinations.

RESULTS: The diets fed to the Cu+Mo groups were roughly equivalent to 25 mg Cu and 5.7 mg Mo/kg DM. Mean initial concentration of Cu in liver for all groups was 516 (SE 54) μmol Cu/kg fresh tissue. In cows supplemented with CS and CG, the final (Day 69) concentrations increased (p<0.01) to 939 (SE 166) and 853 (SE 163) μmol Cu/kg, respectively. These values were not different (p=0.72). For groups CS+Mo and CG+Mo, the final concentrations of 535 (SE 122) and 453 (SE 102) μmol Cu/kg were not different from initial values or from each other (p>0.25). The rate of accumulation of Cu in liver following bolus Cu and Mo intake was highly variable but was not affected by initial concentration of Cu in liver (p>0.9) or by the form of Cu (p>0.6). Mean rates of accumulation of Cu in liver were 4.0 (SD 3.8) and 0.65 (SD 2.0) μmol Cu/kg fresh tissue/day for the Cu-only treatments and the Cu+Mo treatments, respectively.

CONCLUSIONS: When fed together as a bolus, high Mo intake negated the effect of supplemental Cu but it did not reduce liver Cu stores. There was no difference in the reaction of dietary Mo with chelated Cu (as glycinate) versus inorganic Cu (as sulphate) dietary supplements.  相似文献