New insights into radiata pine seedling root infection by Fusarium circinatum          下载免费PDF全文

N. Martín‐Rodrigues  J. Sanchez‐Zabala  I. Salcedo  J. Majada  C. González‐Murua  M. K. Duñabeitia 《Plant pathology》2015,64(6):1336-1348

Pine root infection by Fusarium circinatum has been reported in the literature, but the underlying pathogenic interaction is poorly understood. A green fluorescent protein (GFP)‐tagged F. circinatum isolate, together with confocal microscopy, was used in order to monitor the events associated with root infection of Pinus radiata seedlings. It was found that in order to reach and successfully infect pine roots, F. circinatum employed features that are similar to those previously described for other root‐infecting pathogens, such as mycelial strands, single runner hyphae and simple hyphopodia as well as other features that are reminiscent of those that are known to be involved in biotrophic invasion, such as bulbous invasive hyphae and filamentous invasive hyphae. Abundant sporulation was observed at the root surface as well as inside tracheids both in roots and in the root collar region. The fungus can spread from the roots to the aerial parts of the plant, and once there, colonization appears to be similar to the process that occurs when the pathogen is inoculated in the stem. Wilting symptoms and plant demise may be the result of a reduction in water uptake by roots and of the blockage of the vascular system by fungal hyphae and resin.  相似文献   

菜薹种质遗传多样性的荧光MFLP标记分析     

郭培国  许兰桂  夏岩石  黄红弟  张华  郑岩松  李荣华 《园艺学报》2015,42(2):350-360

采用M13通用接头连接锚定引物,建立了适合于菜薹的荧光微卫星锚定片段长度多态性（MFLP）技术;在此基础上,从360对选择性扩增引物组合中筛选出9对适宜的引物组合,对收集的32份菜薹种质进行等位基因多态性分析;结果显示这些引物组合的扩增产物在32份菜薹种质中的等位基因多态性在10 ~ 31之间,平均为17.7个;多态性信息量在0.61 ~ 0.98之间,平均0.81。利用获得等位基因多态性进行的遗传相似性分析表明,32份菜薹种质间的相似系数在0.277 ~ 0.836之间,平均0.624;基于多态性数据,运用除权配对法（UPGMA）进行聚类分析,将这些菜薹种质材料分为2个组群和4个亚群。这些结果显示荧光MFLP标记技术能有效地发现供试菜薹种质材料的DNA多态性,表明该技术在菜薹遗传特性分析的研究和应用领域具有可行性。  相似文献   

盐酸克伦特罗荧光定量检测试剂盒质量的评价     

葛怀礼  王莲  李金卿  郑百芹  李爱军  张建民  董李学 《中国畜牧兽医》2014,41(10):285-289

本研究使用盐酸克伦特罗荧光定量检测试剂盒,基于免疫荧光层析原理,实现对猪尿中所含克伦特罗的定量检测,并对试纸条的灵敏度、准确度与精密度、特异性、稳定性等进行了初步的性能评价。结果表明,以四参数法对定量曲线进行拟合,线性系数为R²=0.99972;检测限达到0.1 μg/L;对3个不同浓度的添加回收试验,测得其回收率为80%~120%;批内变异系数分别为12.2%、11.1%、15.2%;与4种β-肾上腺素受体激动剂和8种常用的抗生素交叉反应率均低于0.01%;同时验证了本试剂盒在1年的保存期限有效。整个检测过程只需15 min。  相似文献   

Epiphytic colonization of Ustilaginoidea virens on biotic and abiotic surfaces implies the widespread presence of primary inoculum for rice false smut disease     

J. Fan  X.‐Y. Guo  F. Huang  Y. Li  Y.‐F. Liu  L. Li  Y.‐J. Xu  J.‐Q. Zhao  H. Xiong  J.‐J. Yu  W. Wang 《Plant pathology》2014,63(4):937-945

Ustilaginoidea virens (Uv), the causative agent of rice false smut disease, infects developing rice spikelets at the booting stage, and transforms individual grains of the panicle into smut balls. Epidemics of the disease occur when the rice booting and heading stages coincide with rainy days. Using a green fluorescent protein (GFP)‐labelled Uv isolate that can form false smut balls on rice panicles, it was found that under high humidity and free water conditions the Uv isolate could colonize leaves of plants belonging to various families including the Poaceae (Oryza sativa, Echinochloa crusgalli, Digitaria sanguinalis and Leptochloa chinensis), the Brassicaceae (Arabidopsis thaliana) and the Solanaceae (Nicotiana benthamiana) without symptoms. Over several days, some conidia could germinate on the leaves of these plants and in water on the surface of Parafilm and cellophane, form hyphae and differentiate conidiophores to generate a large number of secondary conidia, while other conidia were able to directly produce secondary conidia. Conversely, in the absence of water some conidia could either bud to form new conidia or were converted into chlamydospores. These data indicate that Uv is one of a few fungal pathogens reported to have epiphytic characteristics. The rapid generation of a large number of spores on biotic and abiotic surfaces greatly increases the inoculum that can infect rice spikelets, resulting in the occurrence of rice false smut disease epidemics. These findings are important in the development of disease control strategies.  相似文献   

大丽轮枝菌侵染抗感棉种的组织学过程观察     

肖红利  孔志强  包郁明  赵凤轩  柳少燕  李蕾  陈捷胤  戴小枫 《植物病理学报》2014,44(3):287-294

 棉花黄萎病是一种极难防治的土传性真菌病害,研究病原菌侵染棉花的组织学过程对致病机理解析和抗病资源利用具有重要意义。本研究利用绿色荧光蛋白标记的大丽轮枝菌系统研究了其对抗病棉种海岛棉7124和三裂棉、感病棉种军棉1号和戴维逊棉的侵染过程。结果表明,大丽轮枝菌对抗/感棉种的初始侵染没有明显差异,接菌5 h后,分生孢子均能吸附在感病和抗病棉种的根表面。但侵染过程存在显著差异,侵染感病棉种中病原菌3~5 d到达皮层,5~7 d达到维管束,随后迅速扩展繁殖,侵染14 d后即完成系统侵染,并开始产生黄萎病症状;而病原菌侵染抗病棉种,5~7 d才侵入皮层,7~10 d到达维管束,14 d后仍无法扩展,病原菌的定殖与发展受到限制,无法形成系统侵染,较少形成黄萎病症状。本研究通过绿色荧光蛋白标记大丽轮枝菌对抗/感棉种的侵染过程研究,为大丽轮枝菌致病机理研究和抗性资源利用提供了强有力的理论依据。  相似文献   

食品中柑橘红2号与罗丹明B及苏丹红Ⅰ~Ⅳ的高效液相色谱检验方法     

吴坤 《农产品加工．学刊》2014,(10)

建立高效液相色谱同时测定食品中多种脂溶性染料的含量测定,脂溶性着色剂用正己烷提取后经中性氧化铝固相萃取柱净化,用反相色谱柱分离,高效液相色谱仪测定,外标法定量。样品前处理简单快捷,分离度高,灵敏度高且结果准确可靠,大大提高了实验室的工作效率。  相似文献   

慢性冠心病患者肺炎衣原体血清抗体的检测     

李涛黄建平  许香广张国良吴正林  胡雪松方卫华李松  吴剑胜 《湛江医学院学报》2004,22(2):135-136,140

目的观察慢性冠心病与肺炎衣原体感染的关系.方法应用间接微量免疫荧光法测定慢性冠心病74例(冠心病组)与健康人72例(对照组)血清中肺炎衣原体IgG、IgM的抗体滴度.结果两组的血清肺炎衣原体IgG抗体滴度差异有非常显著性(P＜0.01),对照组与冠心病组肺炎衣原体总感染率分别为72%与100%,其中既往感染率分别为72%与80%,急性感率分别为0%与20%.结论慢性冠心病患者肺炎衣原体感染率增高,肺炎衣原体感染可能是冠心病发生的致病因素之一.  相似文献   

Quinolone-resistance in Salmonella is associated with decreased mRNA expression of virulence genes invA and avrA, growth and intracellular invasion and survival     

Wang YP  Li L  Shen JZ  Yang FJ  Wu YN 《Veterinary microbiology》2009,133(4):328-334

  相似文献   

绿色荧光蛋白基因标记嗜水气单胞菌的研究     

李静  张晓露  刘永杰  陆承平 《畜牧与兽医》2009,41(3)

嗜水气单胞菌广泛存在于水体环境中,可引起人和动物发病,尤其是引起鱼类的败血症。本文构建了pWSK129-gfp重组载体,并分别导入到嗜水气单胞菌强毒株J-1和无毒株4332中。在荧光显微镜下观察,菌体呈现绿色荧光。稳定性试验表明,Ah4332GFP传至70代,质粒稳定率可达100%;而AhJ-1GFP在同样条件下培养10代后,质粒的稳定率仅为15.1%。绿色荧光蛋白基因的导入为研究嗜水气单胞菌与宿主的相互关系提供了一种简单而直观的方法。  相似文献   

Identification of the spontaneous 7BS/7RL intergenomic translocation in one F₁ multigeneric hybrid from the Triticeae tribe     

A. Carvalho    A. Martín    J. S. Heslop-Harrison  H. Guedes-Pinto    J. Lima-Brito 《Plant Breeding》2009,128(1):105-108

The F1 AABBRH^ch hybrids studied here were produced by crosses between the Portuguese triticale cultivar 'Douro' (AABBRR) and the tritordeum line HT9 (AABBH^chH^ch). Fluorescent in situ hybridization performed with genomic DNA probes genomic in situ hybridization (GISH) from rye and Hordeum chilense allowed the unequivocal parental genomes discrimination in all hybrids. Among 55 plants, one presented a spontaneous wheat–rye translocation which was successfully detected after GISH. Recombinant chromosomes identification was made after reprobe with pTa71 and pSc119.2. Nine rDNA loci were detected by pTa71 and pSc119.2 identified the chromosome arms involved in the translocation, after comparing the observed hybridization patterns with those described by several authors. We identified the spontaneous wheat–rye translocation as being the 7BS/7RL. Many wheat–rye translocations have been found (e.g. 1BL.1RS and 1AL.1RS), but as far as we know, this is the first time that this translocation is reported. We considered it helpful for wheat breeding programmes as it could provide the transference of interesting agronomic characteristics from rye (e.g. leaf rust resistance) to wheat.  相似文献