小麦Waxy蛋白亚基缺失类型鉴定方法的研究   总被引：2，自引：0，他引：2  

张红骥  吕晓波  于德才 《黑龙江农业科学》2004,(6):8-9

建立适于我省面条专用小麦育种研究上应用的Waxy蛋白亚基缺失类型的鉴定技术;以改良十二烷基硫酸钠聚丙烯酰胺凝胶电泳技术(1-D-SDS-PAGE)为基础,分析凝胶性质、试验试剂、试验条件对电泳结果的影响,获得高效低成本、分辨效果佳、重复性好的鉴定方法。  相似文献   

The conformation of wheat gluten proteins. The secondary structures and thermal stabilities of α-, β-, γ- and ω-Gliadins     

Arthur S. Tatham  Peter R. Shewry 《Journal of Cereal Science》1985,3(2):103-113

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水牛梭形住肉孢子虫抗原的薄层等电聚焦行为分析     

胡庭俊  罗金印 《中国兽医学报》1997,17(2):180-181

应用薄层聚丙烯酰胺等电聚焦电泳（ＩＦＥ）对水牛梭形住肉孢子虫（Ｓａｒｃｏｃｙｓｔｉｓｆｕｓｉｆｏｒｍｉｓ）抗原进行了分析。结果表明，经ＳｅｐｈａｄｅｘＧ－１００纯化的包囊抗原在电泳图谱上显示３条相距较近的带，等电点分别为６．３０，６．４５，６．６５；缓殖子纯化抗原仅出现１条带，等电点为６．４０  相似文献   

Comparison of the Value of Pulsed-field Gel Electrophoresis,Random Amplified Polymorphic DNA and Amplified rDNA Restriction Analysis for Subtyping Taylorella equigenitalis     

Kagawa  S.  Moore  J.E.  Murayama  O.  Matsuda  M. 《Veterinary research communications》2001,25(4):261-269

Eight strains of Taylorella equigenitalis were identified by a polymerase chain reaction using a primer pair specific to the 16S rDNA of T equigenitalis. These eight strains were chosen because they had previously been shown to represent eight distinct genotypes by pulsed-field gel electrophoresis analysis after separate digestion of the genomic DNA with ApaI or NotI. The eight strains could be classified into six or seven types by random amplified polymorphic DNA analysis using different kinds of primers. Amplified rDNA restriction analysis after separate digestion with five restriction enzymes, including AluI and MboI, of the 1,500 bp fragments of rDNA amplified by polymerase chain reaction did not discriminate the genomic variations among the eight strains of T equigenitalis. Thus, pulsed-field gel electrophoresis was shown to discriminate these eight organisms better than random amplified polymorphic DNA analysis, while amplified rDNA restriction analysis was found to be unsuitable for subtyping T equigenitalis.  相似文献   

毛细管区带电泳测定饲料添加剂中酪蛋白磷酸肽含量的方法研究     

王利民  郝艳红  王静  李景鹏 《黑龙江畜牧兽医》2001,(3):1-2

利用毛细管区带电泳(CZE)对饲料添加剂中酪蛋白磷酸肽(CPP)样品进行分离，结果显示，用四硼酸钠缓冲溶液(20mmmol/L,pH9.2)及50cm×70μmi.d.石英毛细管柱，在工作电压30kV、检测波长200nm、柱温25℃的条件下，CPP得到较好的分离；同时，采用外标法对饲料添加剂样品中CPP各组分的含量进行了测定。  相似文献   

Changes in meat texture of three varieties of squid in the early stage of cold storage     

MIEKO KAGAWA  MISUZU MATSUMOTO  CHIE YONEDA  TOMIKO MITSUHASHI  KEIKO HATAE 《Fisheries Science》2002,68(4):783-792

ABSTRACT: The present study examined the changes in texture and protein components during cold storage of different squid varieties. Raw oval squid, Japanese common squid and arrow squid were sliced fresh and the muscles were stored at 4°C for 0, 4, 8, 12, 18, 24, 48 and 120 h. The rheological measurements, protein components and amounts of collagen were examined. The adhesiveness of each squid increased significantly in the early stage of cold storage. In all varieties, penetration decreased at 4 h, which is considered to be rigor mortis, then increased. The amounts of total collagen, 20°C water-soluble collagen and 70°C water-soluble collagen did not change significantly in each variety during cold storage. Sodium dodecylsulfate–polyacrylamide gel electrophoresis (SDS-PAGE) pattern showed that the 580 kDa component gradually disappeared up to 48 h. The correlations between the amounts of 580 kDa component and adhesiveness or firmness were high. Models of fit based on chemical kinetics accurately expressed the behavior of adhesiveness, firmness and penetration showing that 63.2% of adhesiveness changes occurred in 13–19 h and that 63.2% of firmness changes occurred in 18–24 h.  相似文献   

Meiotic and Isozymic Analyses of Tall Fescue × Giant Fescue Hybrids and Amphiploids1     

G. C. Eizenga  D. M. Burner  R. C. Buckner 《Plant Breeding》1990,104(3):202-211

The meiotic behavior of three tall fescue (Festuca arundinacea, 2n = 6x = 42) genotypes, giant fescue (F. gigantea, 2n = 6x = 42), and their reciprocal F₁ hybrids and C₁, amphiploids was evaluated to determine the parental genomic relationships. Isozyme banding patterns were used to confirm the parental identity of the hybrids and amphiploids. At meta-phase I, the parents had predominantly bivalent pairing. The hybrids had an average of 9.51 I, 16.02 II, 0.12 III, 0.02 IV, and the amphiploids had 2.17 I, 38.82 II, 0.60 III, 0.58 IV, 0.01 V—VIII. The prevalence of bivalent pairing in both hybrids and amphiploids suggested a homoeologous relationship between the six genomes, with four of the six being more closely related. Bivalent pairing in the amphiploids indicated genetic regulation of chromosome pairing. Zymograms were obtained for acid phosphatase (ACPH), alcohol dehydrogenase (ADH), glutamate oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6-PGD) and phosphoglucoisomerase (PGI). The three tall fescue and giant fescue parents had different zymograms for ACPH, MDH, 6-PGD and PGI; thus, the tall fescue parents of the hybrids and amphiploids could be determined based on the banding patterns of these four enzymes. Phenotypes were determined for ACPH-1, PGI-2 and 6-PGD-1. ACPH-1 may be used to follow the introgression of giant fescue chromatin into a certain tall fescue genotype.  相似文献   

应用核酸斑点杂交法检测鹅细小病毒(GPV)   总被引：5，自引：0，他引：5  

余兵  王永坤  朱国强 《中国兽医学报》2002,22(5):453-454

利用鹅细小病毒 (GPV)核酸探针 ,设计并建立了核酸斑点杂交法 ,用以检测 GPV核酸。该方法特异性与敏感性均较好 ,其敏感度可达 3pg。应用该方法对 4例病变典型的小鹅瘟患鹅组织器官进行检测 ,结果表明 ,患鹅脏器中 ,小肠、心、肝、胰、脾组织含毒量较高 ,其中小肠组织含毒量最高 ,而脑组织中含毒量较低。应用琼脂扩散试验验证了核酸斑点杂交法的检测结果。  相似文献   

家蚕血液酯酶同功酶的研究   总被引：3，自引：0，他引：3  

杨瑞丽  徐静斐 《蚕业科学》1990,(1)

采用不连续聚丙烯酰胺凝胶电泳法.测定了24个家蚕品种及杂交种从三龄到后蛹不同发育时期的血液酯酶同功酶酶谱.结果表明:家蚕血液中存在着丰富的酯酶同功酶酶带类型,整个试验可观察到22条酶带,根据其迁移率及染色特点可分为A、B、C、D、E五大染色区.在不同发育阶段中共有13条酶带经常显现,后蛹期主要同功酶酶带活性降低,但非主带A-1区酶带却清楚地显示出来.在幼虫阶段,酶带的数目及活性防着食下量的增多而增人.并观察到A-2-b带与B-2带的表现最为突出,推测A-2-b带可能是家蚕血液部酶同功酶的基本酶带.进一步分析表明,A-2-b带活性与大多数经济性状间呈高度的正相关性.  相似文献   

A Possible Association Between Heading Time and the GM-A2 Locus in Bread Wheat     

D. Laflandra    B. Margiotta  E. Porceddu 《Plant Breeding》1987,99(4):333-335

A line without expression (null form) o: the α-gliadins controlled by the 6A chromosome was found when screening electrosboretically single seeds of the bread wheat ‘Raeder’. Differences in heading time were also observed between this null line and the normal one. A linkage between 6A gliadin genes and heading time gene(s) is hypothesized.  相似文献