不同剂量激素处理对小鼠超数排卵及产仔性能的影响     

周晓旭  李雁冰  宁博林  孙蕊  郭丽  李庆达  韩静  李井春 《黑龙江八一农垦大学学报》2015,(3)

研究不同激素组合处理对小鼠超数排卵及产仔性能的影响,以便获得适宜的超数排卵的激素使用剂量。分别用不同剂量孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)注入小鼠体内进行超数排卵处理,比较激素处理后小鼠卵母细胞成熟率、产仔数、胎儿出生重及性别比。结果显示,5 IU PMSG+5 IU HCG剂量组和10 IU PMSG+10 IU HCG剂量组合相比较,卵母细胞成熟率差异不显著(P0.05),5 IU PMSG+5 IU HCG剂量组平均产仔数最高;10 IU PMSG+10 IU HCG剂量组的胎儿平均初生重最高,但产仔数少。综合以上,在实验中,5 IU PMSG+5 IU HCG剂量组合进行超排处理效果最好。  相似文献   

鲟鱼GtH放射免疫测定方法的建立及催产前后史氏鲟血清GtH含量的变化   总被引：1，自引：1，他引：0       下载免费PDF全文

胡红霞  刘晓春  朱华  张勇 《水产学报》2008,32(6):817-824

用蛋白质快速液相层析(FPLC)分离俄罗斯鲟鱼脑垂体促性腺激素（GtH），共获得GtH纯化蛋白4.6mg。用SDS-PAGE变性胶电泳图谱初步分析纯化蛋白，用反相高效液相色谱（rpHPLC）分离出两个亚基，质谱测定α亚基分子量：15603KD，两个β亚基分子量分别为14338KD和14694KD。制备了鲟鱼GtH的兔抗血清多克隆抗体，采用氯胺T法用125I标记抗原，用羊抗兔γ球蛋白做二抗，建立鲟鱼GtH的放射免疫测定方法。测定了催产前后不同时间史氏鲟血清中GtH含量的变化，统计分析表明成功排卵的雌鱼比未排卵和阴性对照组在催产后12小时和16小时GTH分泌量升高显著，最高达到234ng/ml；催产效应时间与催产后血清中GtH的含量紧密相关。  相似文献   

Some perspectives on fish endocrinology     

Howard A. Bern 《Fish physiology and biochemistry》1989,7(1-6):415-418

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Ovum pick-up and in vitro maturation in spotted paca (Cuniculus paca—Linnaeus, 1766)     

Felipe Farias Pereira da Câmara Barros  Pedro Paulo Maia Teixeira  Luciana Cristina Padilha-Nakaghi  Ricardo Andres Ramirez Uscategui  Marina Ragagnin Lima  Victor José Correia Santos  Kayan da Cunha Rossy  Luísa Pucci Bueno Borges  Marcia Rita Fernandes Machado  Wilter Ricardo Russiano Vicente 《Reproduction in domestic animals》2020,55(4):442-447

We tested FSHp, eCG and FSHp + eCG to establish ovum pick-up (OPU) and in vitro maturation method in spotted paca. Eight healthy adult females were subjected to each of four treatments to stimulate ovarian follicular growth. All females were subjected to a hormonal protocol using a single dose of 45 mg of injectable progesterone and single intramuscular injection of 0.075 mg d-cloprostenol on day 6. Ovarian stimulation was carried out as follows: in Group TFE (FSHp and eCG), animals were treated with a single dose of 80 mg of FSHp and 200 IU of eCG intramuscularly on day 6 after the application of progesterone; in Group TF (FSHp), they were treated with a single dose of 80 mg of FSHp intramuscularly on day 6 after application of progesterone; in Group treatment eCG, they were treated with 200 IU of eCG intramuscularly on day 6 after application of progesterone; and in Group TC (saline solution), 1 ml of saline solution was administered to control does. The OPU was performed between 22 and 26 hr after gonadotropin treatments. All recovered oocytes were placed into maturation media and incubated for 24 hr. There were no differences among the mean number of observed follicles, aspirated follicles and oocytes recovered per treatment. Oocyte maturation rates did not differ among groups, except, TF and treatment eCG oocytes had greater maturation rates than TC oocytes. In this study, gonadotropin administration failed to superovulate treated does and increase oocyte retrieval efficiency. Despite the feasibility of the procedure, further studies are needed to develop and refine hormonal protocols for oocyte recovery and in vitro maturation in this species.  相似文献   

Modulation of glycoprotein hormone α- and gonadotropin IIβ-subunit mRNA levels in the pituitary gland of mature male African catfish, Clarias gariepinus     

F.E.M. Rebers  C.P. Tensen  R.W. Schulz  H.J.Th. Goos  J. Bogerd 《Fish physiology and biochemistry》1997,17(1-6):99-108

The cDNAs encoding the glycoprotein hormone -subunit (GP) and the gonadotropin II-subunit (GTH II) were cloned from the pituitary gland of the African catfish, Clarias gariepinus. Using RNase protection analysis, we studied the steady-state mRNA levels of GP as well as GTH II in the pituitary gland of adult male catfish. Castration of adult male catfish resulted in a significant decrease of GTH II mRNA levels, whereas there was no change in the GP mRNA levels. Treatment of intact males with a single dose of 11-ketotestosterone (11-KT) resulted in dose-dependent increases in mRNA levels of both GP and GTH II. We conclude that 11-KT, a prominent, non-aromatizable teleost androgen, has a stimulatory effect on the pituitary mRNA levels of GP and GTH II of adult male fish.  相似文献   

Positive feedback control by the gonads on gonadotropin (GTH) and gonadoliberin (GnRH) levels in experimentally matured female silver eels,Anguilla anguilla     

Sylvie Dufour  Nadine Le Belle  Sylvie Baloche  Yves-Alain Fontaine 《Fish physiology and biochemistry》1989,7(1-6):157-162

Treatment of sham-operated female silver eels with carp pituitary extract stimulated ovarian development and induced increases in pituitary gonadotropin (GTH) and gonadoliberin (GnRH) contents. Both effects of carp pituitary extract were abolished in ovariectomized eels, indicating the involvement of the gonads. Endogenous sexual steroids, the secretion of which was increased during sexual maturation, should be responsible for the stimulation of GTH and GnRH levels. Ovariectomy itself had no significant effect on pituitary GTH and GnRH contents, reflecting the fact that, at the silver stage, sexual steroid levels are too low to exert any significant effect on pituitary GTH and GnRH. The positive feedback control exerted by the gonads on GTH and GnRH levels during sexual maturation, in the eel as well as in some other teleosts, would produce an amplification of the pubertal stimulation of the hypothalamo-pituitary-gonadal axis.  相似文献   

Mechanisms of synthesis and action of 17α,20β-dihydroxy-4-pregnen-3-one,a teleost maturation-inducing substance     

Yoshitaka Nagahama  Masakane Yamashita 《Fish physiology and biochemistry》1989,7(1-6):193-200

This article briefly reviews the current status of investigations, mainly based on the amago salmon,Oncorhynchus rhodurus, on the mechanisms of synthesis and action of 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-diOHprog). Pituitary gonadotropin is of primary importance in triggering meiotic maturation in teleost oocytes. However, the maturational action of gonadotropin is not direct, but is mediated by the follicular production of maturation-inducing substance (MIS). It is now well established that 17α,20β-diOHprog is the major MIS of salmonids. Production of this steroid occursvia the interaction of two distinct cell layers, the thecal and granulosa cell layers (2-cell type model). The first step of the stimulating effect of gonadotropin in both layers is the receptor-mediated activation of adenylate cyclase and formation of cAMP. Our findings suggest that the major stimulating action of gonadotropin on 17α,20β-diOHprog biosynthesis is due to the stimulation of 17α-hydroxyprogesterone production by the thecal layer and the selective induction of thede novo synthesis of 20β-hydroxysteroid dehydrogenase in the granulosa layer. 17α,20β-diOHprog acts at the surface of the oocyte. The early steps following 17α,20β-diOHprog action involve the formation of the major cytoplasmic mediator of this steroid, maturation-promoting factor (MPF). It was shown that goldfish MPF induces meiotic maturation inXenopus oocytes andvice versa. The chemical characterization of fish MPF is important for our understanding of the precise mode of maturational action of 17α,20β-diOHprog.  相似文献   

Neuropeptide Y (NPY) modulatesin vitro gonadotropin in release from rainbow trout pituitary glands     

Bernard Breton  Tomasz Mikolajczyk  Jean-Michel Danger  Francis Gonnet  Serge Saint-Pierre  Hubert Vaudry 《Fish physiology and biochemistry》1989,7(1-6):77-83

This work investigated the action of neuropeptide Y (NPY) on thein vitro pituitary release of the maturing gonadotropic hormone (GtH) of the rainbow trout using a perifusion system employing trout balanced salt solution (pH 7.5) at 15°C and a 12.5 ml/h flow rate. In vitellogenic females a 20 minutes NPY application (10⁻⁷ M) induced a 20–30% decrease in GtH secretion. Removal of NPY was followed by a rebound in GTH secretion. On the contrary, in ovulated females, NPY (15 minutes, 10⁻⁷ M) directly stimulated GTH secretion. The greatest stimulation was obtained the day of ovulation where the stimulatory effect of NPY was similar to those induced by s.GnRH in the same conditions, reaching 400% of the basal GTH level. In vitellogenic females treated with 1-4-6 androstadien 3–7 dione, an inhibitor of aromatase activity, the pituitary response to NPY was similar to that obtained in ovulated females. Thus thein vitro action of NPY might depend on thein vivo steroidogenic environment.  相似文献   

Fluctuations in gonadotropin and ovarian steroids during the annual cycle and spawning of the common carp     

Zvi Yaron  Berta Levavi-Zermonsky 《Fish physiology and biochemistry》1986,2(1-4):75-86

The main objective of the paper is to describe the annual changes in hormones associated with reproduction in the female carp under the conditions prevailing in the Israeli fish culture. Fish were sampled monthly throughout 1984; gonadosomatic index (GSI) was calculated and the diameter of ovarian follicles was measured. Gonadotropin (GTH) content in the pituitary and the circulating GTH, estradiol, testosterone and 17, 20-dihydroxy-4-pregnen-3-one (17, 20-P) were determined by specific radioimmunoassays. GTH, estradiol and testosterone showed a bimodal annual pattern. The late summer peak was associated with initial vitellogenesis while the peak in spring occurred just before spawning, which took place in April-May. A resting phase in ovarian activity was noted in June and July. The levels of 17, 20-P were very low compared with those occurring during spawning induction. The paper summarizes a previous study by our laboratory on the changes in circulating hormones, as related to oocyte stages, in female carp induced to spawn by a GTH-calibrated pituitary extract. This study associates the short but prominent peak in 17, 20-P with the presence of follicles with maturing oocytes in the ovary. A correlation was found between the percentage of oocytes with eccentric germinal vesicle initially present in ovarian biopsies of females carp and their spawning success after hypophysation. The paper describes simple means to ensure successful induction of spawning in carp by utilizing a calibrated pituitary extract and prior selection of females that would respond to the induction treatment.  相似文献   

人绒毛膜促性腺激素(HCG)对杂交黄牛、水牛受胎率的影响     

吴师  李华慧  覃现才  宾娟容 《畜牧与饲料科学》2011,(2):66-67

近年来,广西象州县的多个牛人工授精品改点的受胎率,黄牛为33%～51%,水牛为35%～55%,母牛的受胎率仍有较大的提升空间。该试验利用HCG能够促进母牛卵泡发育和排卵这一特性,在母牛发情时肌肉注射HCG后进行输精,并观察其对母牛受胎率的影响,以及是否能够提高牛人工授精的效果。试验结果表明,使用HCG能够明显提高牛人工授精的受胎率,同时也提高了工作效率。  相似文献