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41.
以鼠曲草为原料,采用水提法提取鼠曲草中的黄酮类物质,再将鼠曲草水提液、柠檬酸、白砂糖、羧甲基纤维素钠进行调配,制作鼠曲草黄酮饮料。在单因素试验的基础上,通过正交试验分别对鼠曲草黄酮水浸提工艺及鼠曲草黄酮饮料配方进行优化。结果表明,鼠曲草黄酮的最佳浸提工艺为:浸提料液比1∶130(g/mL),浸泡时间30 min,提取时间2.5 h,浸提温度80℃;鼠曲草黄酮饮料最佳配方为:以100 m L饮料计,鼠曲草水提液添加量40 mL,白砂糖添加量9 g,柠檬酸添加量0.05 g,羧甲基纤维素钠添加量0.05 g。按此配方制备的饮料可溶性固形物含量8.39%,总黄酮含量6.56 mg/100 mL。按上述工艺配方制备的鼠曲草黄酮饮料呈青绿色,具有鼠曲草的清香,酸甜适口。  相似文献   
42.
为了筛选对草地贪夜蛾防效优良的新型药剂,采用浸卵法和浸叶法在室内研究了10%硫虫酰胺SC对草地贪夜蛾的毒力,并用喷雾法进行了田间控制作用研究。结果表明,硫虫酰胺浸卵处理对草地贪夜蛾的卵孵化率无明显影响,但能显著提高初孵幼虫的死亡率,其中66.7、133.3、266.7 mg/L等3个浓度处理初孵幼虫校正死亡率均高于92.77%;硫虫酰胺对草地贪夜蛾3龄幼虫有较强的毒杀作用,各处理72 h后的校正死亡率为65.55%~100%,但对5龄幼虫防效差。田间药效试验表明,药后1 d各处理防效为61.56%~76.75%,速效性差于对照药剂5.7%甲氨基阿维菌素苯甲酸盐(甲维盐)ME;药后7 d各处理防效为84.84%~95.82%,持效性优于5.7%甲维盐ME和200 g/L氯虫苯甲酰胺SC。研究表明,10%硫虫酰胺SC可以有效控制草地贪夜蛾,且持效性较好,建议每667 m~2制剂用量30 mL左右,防治适期为草地贪夜蛾卵孵盛期及3龄幼虫前。  相似文献   
43.
Flavobacterium psychrophilum is the causative agent of bacterial cold-water disease and rainbow trout syndrome in freshwater salmonid fish worldwide, generating injuries and high mortality rates. Despite several studies on this bacterium, the infection mechanism remains unknown due to limitations in the employed animal models. In this work, we propose using zebrafish (Danio rerio) as a model for studying bacterial pathogenicity. To substantiate this proposal, zebrafish infection by F. psychrophilum strain JIP 02/86 was characterized. Zebrafish larvae were infected using the bath method, and morphological changes and innate immune system activation were monitored using transgenic fish. Salmonid-like infection phenotypes were observed in 4.74% of treated larvae, as manifested by fin, muscle and caudal peduncle damage. Symptomatic and dead larvae accounted for 1.35% of all challenged larvae. Interestingly, infected larvae with no infection phenotypes showed stronger innate immune system activation than specimens with phenotypes. A failure of function assay for myeloid factor pu.1 resulted in more infected larvae (up to 43.5%), suggesting that low infection rates by F. psychrophilum would be due to the protective actions of the innate immune system against this bacterium in zebrafish larvae. Our results support the use of zebrafish as an infection model for studying F. psychrophilum. Furthermore, the percentage of infected fish can be modulated by disturbing, to varying extents, the differentiation of myeloid cells. Using this evidence as a starting point, different aspects of the infection mechanism of F. psychrophilum could be studied in vivo.  相似文献   
44.
The temperature requirements of three single cystosorus strains of Polymyxa sp. from India were studied at 15–18, 19–22, 23–26 and 27–30 °C (night-day temperature), and compared with the temperature requirements of three strains of P. graminis from Belgium, Canada and France and two strains of P. betae from Belgium and Turkey. Sorghum was used as the host-plant for the Indian strains; the strains of P. graminis and P. betae from temperate areas were cultivated on barley and sugar beet, respectively. The cystosori germination and the development of plasmodia, zoosporangia and cystosori of Polymyxa sp. from India were optimal at 27–30 °C. Infection progression was slower at 23–26 °C than at 27–30 °C. At 19–22 °C, infection was insignificant. No infection occurred below 19 °C. In contrast, the infection of barley with P. graminis strains from temperate areas was optimal at 15–18 °C, but at 19–22 °C the progression appeared inconsistent and infection stayed low. Above 22 °C, infection was insignificant. P. betae strains showed consistent infection in the range of 15–18 °C to 27–30 °C. Plasmodia formation and cystosori detection of the Belgian strain were slightly advanced at 23–26 °C compared to 19–22 °C but clearly restrained at 27–30 °C. Fungus development of the P. betae strain from Turkey was almost as high at 27–30 °C as at the lower temperatures. These results strengthen the case for distinguishing between Polymyxa sp. from India and P. graminis or P. betae from temperate areas.  相似文献   
45.
Two liquid culture systems, rotary shaker (RS) and temporary immersion system (TIS) for the propagation of Rosa rubiginosa and Rosa tomentosa were investigated and compared with solid medium culture. Shoot tips were grown on basic Murashige and Skoog medium with 20 mg dm?3 Fe EDDHA, 1 µM BA, 1.5 µM GA3 and 3% sucrose for six weeks. Of the different culture systems, liquid cultures resulted in better growth and development of roses. Both RS and TIS cultures improved biomass growth, multiplication rate, leaf blade area and plant height; however, RS reduced the shoot dry mass content. There were no changes in the content of chlorophyll a, b, a + b in plants developed in the tested systems. Use of a solid medium favoured the accumulation of phenolic compounds, soluble sugars, and carotenoids, but it was accompanied by browning and necrosis of shoots. Plants propagated in TIS were characterised by the high content of phenolic compounds and soluble sugars, but they had the highest multiplication rate. Use of TIS resulted in eight times the number of shoots for R. tomentosa and twice as many for R. rubiginosa compared to solid medium.  相似文献   
46.
为了探究导管涡轮机在复杂潮流条件下的非定常性能,依据斋堂岛海域的潮流条件,采用计算流体力学(CFD)数值模拟方法分析了浸没深度和偏流角对导管涡轮机水动力学性能及尾流场流态的影响.研究结果表明:不同浸没深度和偏流角下的导管涡轮机水动力学性能均表现出与波浪频率一致的时间周期性行为;在波流作用下,随着浸没深度的减小,水流将发生强烈的跃迁,由波流引起的水动力学性能波动幅值增大;在波流与偏流共同作用下,随着偏流角的增大,导管涡轮机的平均功率系数及推力系数逐渐减小.此外,水动力学性能在叶轮旋转周期内将出现额外的周期性波动,该波动频率由叶轮转速和叶片数决定,且波动幅值随着偏流角的增加而增大,导管涡轮机后方的尾流场结构也将变得相当不稳定和复杂.  相似文献   
47.
负压结合药剂浸种对辣椒苗期病毒病的防效   总被引:1,自引:0,他引:1  
用0.02,0.04,0.06 MPa三种不同的负压永平,结合10%磷酸三钠药剂处理辣椒种子.试验结果表明,负压结合药剂浸种处理辣椒种子可有效降低辣椒苗期病毒病的发生,特别是在0.06 MPa负压条件下,可大幅度降低辣椒苗期病毒病的为害.  相似文献   
48.
蔬菜中残留农药消减方法研究   总被引:1,自引:0,他引:1  
以菜心为材料,研究了清水、食盐水、洗洁精水溶液分别浸泡菜心15,30,45 min后,对蔬菜残留农药的消减率,经农残快速检测方法(速测卡法)和气谱-质谱联用仪(GC-MS联用仪)法检测,结果表明,1%洗洁精水溶液浸泡15~30min,消减效果最明显,但是经这些溶液浸泡后,蔬菜的食用品质会出现一定程度的下降.  相似文献   
49.
利用OpenGL开发汽车操纵稳定性虚拟试验软件平台   总被引:4,自引:0,他引:4       下载免费PDF全文
在桌面虚拟现实系统(Desk-top Virtual Reality)下,利用OpenGL(Open Graphics Library)开发了“汽车操纵稳定性虚拟试验软件平台”。介绍了虚拟试验的意义、采用OpenGL作为开发虚拟试验软件平台的原因,提出了虚拟试验平台的功能要求,给出了完成其功能的若干关键技术的实现方法。虚拟试验为车辆运动仿真提供了一种新的研究手段。  相似文献   
50.
汽车操纵稳定性的虚拟实验系统   总被引:2,自引:0,他引:2  
建立了基于桌面虚拟现实系统的汽车操作稳定性虚拟实验平台,在该平台上进行了汽车双移线的虚拟实验。结果表明:虚拟实验与实际实验有较好的一致性。  相似文献   
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