Effects of acute hyperglycaemia stress on indices of glucose metabolism and glucose transporter genes expression in cobia (Rachycentron canadum)     

Guanlin Ye  Wucai Zhang  Xiaohui Dong  Beiping Tan  Shuang Zhang 《Aquaculture Research》2020,51(2):584-594

The objective of the present study is to preliminarily clarify the mechanism of carbohydrates metabolism in cobia (Rachycentron canadum) (85 ± 3 g) receiving injection of glucose solution. We examined plasma glucose (GLU), total protein (TP), triglyceride (TG), cholesterol (CHOL), insulin, liver glycogen and muscle glycogen, activities of hepatic hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK), as well as relative expressions of glucose transporter 1 (GLUT1), GLUT2, GLUT3, GLUT4, GLUT5 and GLUT9 in hemocyte, liver and muscle of R. canadum when fish were injected with 200 μl of glucose solution (255 mg/ml) after 0, 1, 2, 4, 8, 12, 24 and 48 hr. Fish received injection of 0.68% saline served as control. Results indicated that the plasma GLU, TG and CHOL increased and reached peak at 1, 8 and 48 hr postinjection (hpi) respectively. The hepatic glycogen increased from 1 hpi, and reached peak at 8 hpi, plasma insulin increased at 1 hpi, and reached peak at 2 hpi, and activity of hepatic PK peaked at 8 hpi. Furthermore, the relative expressions of GLUT1, GLUT2, GLUT3, GLUT4 and GLUT5 in hemocytes reached peak at 1,4, 8, 4 and 8 hpi, respectively, relative expressions of GLUT2, GLUT3, GLUT5 and GLUT9 in liver reached peak at 24, 24, 12 and 24 hpi, respectively, and relative expressions of GLUT1 and GLUT3 in muscle were significantly higher at 2 and 2–4 hpi, respectively compared with those in controls. In conclusion, low ability of utilizing glucose in R. canadum may be attributed to insufficient insulin secretion, low activities of key glycolytic enzymes (HK, PFK and PK) regulated by glucose injection and slow increase of GLUTs.  相似文献   

云纹石斑鱼(♀)×鞍带石斑鱼(♂)杂交后代染色体核型分析          下载免费PDF全文

成美玲  田永胜  吴玉萍  李振通  张晶晶  王林娜  李文升  马文辉  王晓梅  翟介明  李波 《渔业科学进展》2018,39(2):67-75

本研究对云纹石斑鱼(Epinephelus moara♀)×鞍带石斑鱼(Epinephelus lanceolatus♂)杂交后代利用植物血球凝集素(PHA)及秋水仙素通过活体注射法制作染色体标本。选用头肾和鳃组织制作细胞悬浊液,并对冷滴片和热滴片法进行比较,结合空气干燥法制作染色体分裂相玻片,经吉姆萨染色后,在显微镜下观察筛选清晰完整的分裂相。杂交子代(俗称云龙斑)选取75个分裂相,统计染色体数目并进行核型分析。结果显示,云龙斑二倍体染色体数为48,其中,19对染色体为端部着丝粒染色体,2对为亚中部着丝粒染色体,3对为亚端部着丝粒染色体,其染色体臂数(NF)为58,核型公式为2n=48,4sm+6st+38t。杂交后代云龙斑与父母本染色体数目相同,都为2n=48,但云龙斑染色体与其父母本组型不同,在遗传过程中发生了复杂的染色体重新配对和变异。本研究为杂交后代"云龙斑"的种质鉴定、亲缘关系分析、杂交育种等提供了重要的科学根据。  相似文献   

单板层积材喷蒸热压的初步探讨   总被引：7，自引：1，他引：6  

徐咏兰  金菊婉  周晓燕 《林产工业》2000,27(4):22-24

探讨了喷蒸热压制作杉木单板层积材的工艺。研究了热压温度、热压时间、喷蒸时间和涂胶量对单板层积材性能 的影响。结果表明,喷蒸热压可有效缩短杉木单板层积材的热压时间,且制品性能较好。  相似文献   

外源λDNA导入普通小麦雄性不育变异的分子验证   总被引：3，自引：3，他引：0  

杨景成  于元杰  刘凤珍  齐延芳  沈法富 《核农学报》2000,14(6):371-374

将外源λDNA导入普通小麦品系 81 45 2 7,获得一细胞质雄性不育变异体 ,稳定成系 ,简称D型不育系 ,受体 81 45 2 7可作其保持系。用32 P标记的λDNA作为探针 ,对受体 81 45 2 7、D型不育系及其与鲁麦 1 4号的杂种F1 核DNA和叶绿体DNA进行点杂交。实验结果表明 :D型不育系及杂种F1 核DNA和叶绿体DNA点杂交均呈阳性反应 ,而受体无阳性反应。说明外源λDNA片段已经整合进了不育变异体核基因组和叶绿体基因组中 ,并且可以稳定遗传。该结果从分子水平上初步证明了外源DNA导入技术的可靠性  相似文献   

Root competition for phosphorus between coconut, multipurpose trees and Kacholam (Kaempferia galanga L.) in Kerala, India     

S. S. Kumar  B. M. Kumar  P. A. Wahid  N. V. Kamalam  R. F. Fisher 《Agroforestry Systems》1999,46(2):131-146

The magnitude of root competition 17 year-old coconut palms suffer from three year-old inter-planted multipurpose trees, Vateria indica L., Ailanthus triphysa (Dennst.) Alston. or Grevillea robusta A. Cunn. and kacholam (Kaempferia galanga L.), a herbaceous medicinal plant, was evaluated based on the extent of absorption of applied ³²P by the palms in sole and mixed crop situations. The multipurpose tree (MPT) species were grown under two planting geometries (single row and double row). The hypothesis that, when grown together, widespread root proliferation of coconut and multipurpose trees occurs in the well-fertilised kacholam beds was tested by root excavation. Interplanted MPTs substantially altered absorption of ³²P by coconut. Both Ailanthus and Vateria exerted a modest depressing effect, while Grevillea enhanced ³²P uptake by coconut. Single rows of MPTs also favoured ³²P recovery by coconut, presumably because of the increased root densities in the subsoil. Ailanthus, Vateria and Grevillea absorbed substantial ³²P. Overall, high ³²P absorption in the coconut-Grevillea plots indicates complementary root-level interactions between these species. ³²P absorption by MPTs was generally higher closer to the trees owing to the greater root concentration of the MPTs, which in turn suggests possible root interference between MPTs and coconut. Hence selection of tree species with low root competitiveness and/or trees with complementary root interaction is of strategic importance in agroforestry. Kacholam showed substantial ³²P content in its foliage. This ³²P appears to have been translocated by coconut into the kacholam beds where new coconut roots were abundant. This revised version was published online in June 2006 with corrections to the Cover Date.  相似文献   

低排放的直喷式柴油机燃烧室形状的研究     

宫长明  刘巽俊  谭满志  李骏  王恩宇 《农业机械学报》1999,30(1):18-22

针对６１１０直喷式柴油机进气系统和喷油系统的特点,设计了几种缩口燃烧室,研究了燃烧室形状对高压喷射直喷式柴油机性能和排放的影响规律,试验结果表明,缩口燃烧室有利于实现柴油机的动力性,经济性 和排放指标的折衷,特别是创造了氮氧化物排放和微粒排放同时降低的条件;大的底台体积和较长唇部的缩口燃烧室有利于延迟喷油,可以更加有利于氮氧化物和微粒排放的降低,喷油提前角和喷雾锥角决定着燃汪在燃烧室内的落点高度,  相似文献   

兽用中草药注射剂的发展与方剂应用     

谢麟  谢虹 《兽药与饲料添加剂》1999,4(5):26-30

我国有丰富的中草药资源和发展中草药剂的潜力。本文从中草药注射液品种开发与发展中草药注射液的技术问题,结合方剂的应用,试论我国兽用中草药注射剂当前发展的方向与其要点。  相似文献   

树干注射法防治樗蚕试验研究     

李小妮  宋新强  谢杰  冀国军 《河北林果研究》1999,14(3):259-261

为有效防 治樗蚕, 采用 树干 注射 ２５ ％ 灭 幼 脲Ⅲ 号悬 浮 剂, １０ ％ 灭 百可 乳 油, ５０ ％ 甲胺磷乳油 , ２５ ％ 敌 杀死乳 剂等药剂 的 方法, 对樗 蚕１ ～３ 龄 幼 虫进 行 了防 治 试 验。与 对 照相 比,４ 个处理 均达到 极显著水 平, ４ 个 处理之间 也存在 极显著差 异。并 且幼 虫死 亡率 随着 时间 的延长而呈 对数曲线 的趋势 上升。  相似文献   

Branchial cobalt uptake in the carp, Cyprinus carpio: Effect of calcium channelblockers and calcium injection     

S. Comhaire  R. Blust  L. Van Ginneken  P.M. Verbost  O.L.J. Vanderborght 《Fish physiology and biochemistry》1998,18(1):1-13

Using tracer-uptake studies we investigated whether the branchial uptakeof cobalt occurs via selective, inhibitable, calcium uptake routes.Modulation of the calcium transport system was performed using generalcalcium channel blockers (Cd²⁺, La³⁺,Mg²⁺), a voltage-dependent calcium channel blocker (diltiazem)and an intraperitoneal CaCl2 injection. Adding Cd²⁺ orMg²⁺ to thewater or injecting the fish with theCa²⁺-solution resulted in decreased Co²⁺ andCa²⁺ uptake rates. Addition of La³⁺ reducedCa²⁺ and Co²⁺ uptake rates except forCo²⁺ uptake in the gills. Diltiazem displayed only aninconsistent, concentration-independent blocking of the uptake of bothCo²⁺ and Ca²⁺. All inhibitors which inhibitCa²⁺ uptake also inhibit Co²⁺ uptake. However, aclear difference in the degree of inhibition of uptake of both elements isobserved. Two possible explanations are suggested (1) Co²⁺ andCa²⁺ are both transported through the gills via the sametransport system or (2) the calcium transport system is not the only systeminvolved in the transport of Co²⁺.  相似文献   

气流引导式果园注肥机设计与试验     

陈相安  张学敏  侯秀宁  王杰  刘思瑶  倪志明 《农业机械学报》2022,53(10):108-117

针对目前果园液态肥注肥作业存在易伤果树根系、肥料利用率低、注肥不均匀等问题,为实现果园液态肥高利用率、横向均匀扩散施用,设计了一款气流引导式果园注肥机,以高压气流疏松土壤引导液肥扩散。分析了果树注肥作业要求及气流引导注肥作业原理,对注肥装置、注肥气液管路、注肥下压装置进行理论分析及设计。通过气流引导参数注肥参数交互试验进行分析,确定了最优的参数组合为：通气压力0.8MPa,注肥压力1.5MPa。基于虚拟样机技术完成气流引导式果园注肥机的建模并完成样机的试制,在果园进行了田间试验。试验结果表明,注肥机作业效果稳定,无液肥上溢现象,液肥纵向扩散范围264~320mm,横向扩散范围250~270mm,作业效率0.10~0.12hm2/h,满足果园液态肥作业要求。  相似文献