谷精草属六个种的同工酶聚类分析     

陈星  郑彩霞  张志翔 《吉林林业科技》2008,37(3):1-5

本文对谷精草属6个种的POD、EST、MDH和AMY同工酶分别进行了电泳分离,采用STATISTICA/W4.5软件对酶谱的不同相似性系数进行聚类分析,进而绘出树系图。同工酶酶谱及其聚类分析的结果与现有的形态学分类系统基本相符,首先将E.sexangulare Linn.、E.alperstre与E.buergerianum分为一支,将E.kunmingense Z.X.Zhang、E.nepalense Prescottex Bongard与E.cinereum R.Brown并为一支,且它们是平行进化的关系。在第一支中,E.alperstre与E.buergerianum亲缘关系更近。  相似文献   

梨属间及~(60)Coγ射线辐射诱变朝鲜洋梨新品系过氧化物酶同工酶的研究     

李志英  赵紫芬 《果树学报》1991,(2)

试验采用聚丙烯凝胶电泳对梨属的秋子梨、沙梨、西洋梨、杜梨、矮化梨及朝鲜洋梨辐射优系共37个品种(系)嫩枝的韧皮部提取液进行过氧化物酶同工酶的测定,结果表明:梨属植物不同种间的谱带差异较大,种内品种间差异较小。矮化品种及矮化砧的谱带均较复杂,朝鲜洋梨辐射新品系的主要谱带均显现一条强谱带的缺失,或由强带向弱带的变化。由此认为用过氧化物酶同工酶来鉴定梨的种属,品种及新品种(系)的形成是一条可行的途径。  相似文献   

栽培稻籼,粳交F1杂种优势与同功酶表达     

李予先  陈忠友 《种子》1991,(6):17-22

水稻杂种优势的表现,前人已作过部分研究,初步的结论是:水稻的杂种优势具有遗传多样性。那么,灿、粳交F_1代杂种优势有哪些特点呢?本文拟讨论这个问题。 1 材料和方法 1984年组成珍汕97×城堡_2、桂朝_2×城堡_2、珍汕97×密阳_(23)桂朝_2×密阳_(23)桂朝_(13)×密阳_(23)、川新糯×密阳_(23)等6个杂交组合。密阳_(23)是南朝鲜用籼梗交育成的偏籼型品种,城堡_2是日本用籼粳交育成的偏粳型品种。杂交时  相似文献   

泡桐丛枝病与过氧化物酶含量关系的研究初报   总被引：4，自引：1，他引：3  

蒋建平  朱建军 《河南农业大学学报》1993,27(4):301-304

分别采用分光光度法和不连续聚丙烯酰胺凝胶盘状电泳法，比较分析了感病与未感病泡桐树木的过氧化物酶总活性与同工酶的变化。结果表明，泡酮感病后，叶片的ＰＯＤ总活性没有显著变化，枝，根韧皮部的ＰＯＤ总活性，病株高于健株。健株枝部和根部的ＰＯＤ同工酶谱带多于病株，活性也高于后者。可以用ＰＯＤ总活性与同工酶变化两个指标作为判别泡桐感病与束的依据。  相似文献   

海藻多糖对肉鸡生长性能影响的研究   总被引：14，自引：0，他引：14  

朱新产  王宝维  张庭荣 《动物营养学报》2002,14(4):54-58

在肉鸡饲粮中添加1.5 %海藻多糖 ,对山东地方特色保种白羽鸡进行饲养试验 ,用聚丙烯酰胺凝胶电泳(SDS)PAGE技术分析血液中脂酶(EST)、乳酸脱氢酶(LDH)、蛋白质的多态。结果表明 ,处理组90Ku蛋白亚基组分表达程度显著增强。EST同工酶快区Es -1位点 ,由多等位基因控制 ,酶带着色程度显著减弱 ,其活性下降42.7 %。LDH同工酶出现3条酶谱带 ,酶带着色的程度明显增强 ,其活性增加3倍。此外 ,添加组的饲料利用率、增重及生长性能明显提高 ,提示海藻多糖直接或间接调控基因表达水平 ,与生长性状呈正相关。  相似文献   

Family Outcrossing Rates of Pinus caribaea Morelet var. caribaea in Seed Orchard and Natural Populations     

ZHENG YongqiResearch Institute of Forestry. Chinese Academy of Forestry. Beijing 《中国林业科技(英文版)》2002,(1)

Family outcrossing rates of Pinus caribaea var. caribaea populations that differed in origin and management were estimated by using isozyme markers. The family outcrossing rates were estimated by (1)fixing the probability of pollen gene frequency (p); (2) allowing pollen gene frequency to vary among families. The estimates of family outcrossing rates for all the populations varied widely among families (clones of the seed orchard), ranging from 0.39 to 2.0. The average family outcrossing rates of both methods were greater than the  相似文献   

利用EST同工酶谱鉴定黑稻品种纯度的研究   总被引：1，自引：0，他引：1  

丁锐  李利华 《种子》2006,25(4):21-22,26

采用改进的聚丙烯酰胺凝胶电泳对5种黑稻主要栽培品种进行了EST同工酶谱分析.结果表明,此方法分辨率高.测试各品种的EST同工酶存在丰富的多态性,其电泳图谱具有鲜明的特征,并构成了各自的＂指纹＂.其中黑优粘和云黑酶谱条带数多达15条,a1和a3为5个品种的共有谱带.通过EST同工酶谱相似度指数分析,黑优粘与黑丰糯、乌贡1号相似度指数大于0.75,说明这3个品种间亲缘关系较近.  相似文献   

Isozyme analysis of an active cassava germplasm bank collection     

Angélica V. V. Montarroyos  Marco A. G. de Lima  Elton O. dos Santos  José G. E. de França 《Euphytica》2003,130(1):101-106

  相似文献   

Isozyme polymorphism in three gene pools of cultivated chicory (Cichorium intybus L.)     

P. G. Baes  P. J. Van Cutsem 《Euphytica》1993,71(1-2):143-150

Summary Polymorphism of ten enzymes, acid phosphatase (APH), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), phosphorylase (PP), superoxide dismutase (SOD), malic enzyme (ME), glutamate oxaloacetate transaminase 1 and 2 (GOT-1, GOT-2) and phosphoglucomutase 1 and 2 (PGM-1 and PGM-2), was investigated in three gene pools of cultivated chicory, including six cultivated wild chccory, eight industrial chicory and eight Brussels chicory varieties. LAP, APH, PP and PGM-2 showed high phenotypic polymorphism whilst GOT-1 and ME had poor polymorphism. For three enzyme coding loci Lap, Pgm-1 and Got-2, allele frequencies were determined. Isozyme composition in the three chicory gene pools was significantly different, showing, respectively, high, intermediate and poor average amount of phenotypic polymorphism in cultivated wild chicory, industrial chicory and Brussels chicory. Isozyme variation within and between varieties of the three gene pools is discussed in relation to breeding practices.  相似文献   

Isozyme polymorphism in Festuca rubraL.     

V. Livesey  J. Norrington-Davies 《Euphytica》1991,55(1):73-79

Summary Six Festuca rubra populations from Europe and Scandinavia were studied for variation at three isozyme loci; phosphoglucoisomerase (PGI-2), glutamate oxaloacetate transaminase (GOT-3) and superoxide dismutase (SOD-1). Seven alleles were found at the Pgi-2 locus, four at the Got-3 locus and five at the Sod-1 locus. Most plants were heterozygous and up to five alleles were found in the same plant at the Pgi-2 locus. Each population could be distinguished by the presence or absence of certain alleles or by differences in the frequencies of the alleles present. Values for the Shannon diversity index were calculated which showed that there was considerable heterogeneity both within and between loci. In general, 53% of this diversity could be attributed to within population variation.  相似文献