检测胸水与血清的唾液酸、癌胚抗原及乳酸脱氢酶对鉴别良、恶性胸水的价值     

苏伟强  周宁  郭洁莲  梁铭海  梁开  黄洁珍 《湛江医学院学报》1996,(2)

检测良、恶性胸水患者各２０例的血清与胸液的唾液酸（ＳＡ）、癌胚抗原（ＣＥＡ）、乳酸脱氢酶（ＬＤＨ）和４０例健康人的血清ＳＡ的结果表明：（１）良、恶性胸水患者的血清ＳＡ均高于正常人组（Ｐ＜０．０１），但良、恶性两组间比较差异不显著（Ｐ＞０．０５）；（２）良、恶性胸水患者的胸水与血清ＳＡ、ＣＥＡ、ＬＤＨ及ＳＡ＋ＣＥＡ联合，ＳＡ＋ＣＥＡ＋ＬＤＨ联合检测，鉴别良、恶性胸水以ＣＥＡ（胸液／血清比值≥１．５）最优，特异性高达９５％，敏感性为５５％。  相似文献   

杂交玉米及亲本种子碱溶蛋白电泳鉴定   总被引：5，自引：0，他引：5  

孙雁  冯锐  段利琴 《种子》2000,(5):17-18,29

用乳酸-聚丙烯酰胺凝胶电泳对玉米不同杂交种及其亲本种子碱溶蛋白进行分析,结果表明：玉米碱溶蛋白在蛋白质组成上存在丰富的多态性,各杂交种和自交系的电泳谱带差异明显,可视为各自的“特征”指纹,通过指纹分析能有效地将各供试品种区分开来。因此,碱溶蛋白可作为玉米品种鉴定的依据。  相似文献   

Reaktion der Zuckerrübe (Beta vulgaris var. altissima) auf die Kaliumdüngung – ein 20‐jähriger Feldversuch     

Wilhelm Rmer  Norbert Claassen  Bernd Steingrobe  Bernward Mrlnder 《植物养料与土壤学杂志》2004,167(6):726-735

Response of sugar beet ( Beta vulgaris var. altissima ) to potassium fertilization—a 20‐year field experiment A long‐term fertilizer experiment was performed to develop a K fertilization strategy to achieve highest extractable sugar yields (BZE). Sugar beet was grown in a crop rotation with wheat and barley on an alluvial soil (clayic silt) in Lower Saxony with annual recycling of straw and beet tops, respectively. Since 1983, the treatments were as follows: 1) K fertilization with 0, 29, 58, 87,174, and 524 kg K ha^–1 a^–1 corresponding to 0, 0.5, 1, 1.5, 3, and 9 times the average annual K removal by the marketable products of the crop rotation—since 1995, the two highest treatments (3 and 9 times the removal) received only 174 kg ha^–1 every third year; 2) K fertilization according to the average K removal, given each year (58 kg K ha^–1) or every third year (174 kg ha^–1) to sugar beet; 3) annual K fertilization of 87 kg K ha^–1 (1.5 times the removal) applied in autumn or spring, respectively; 4) annual K fertilization, applied as mineral fertilizer or as organic material (recycling of grain and straw or root and leaves); 5) application of 29 kg NaCl ha^–1 to sugar beet supplemental to a yearly application of 58 kg K ha^–1. Both root yield and soil concentration of lactate‐soluble K increased with K fertilization up to the highest K treatment. The extractable sugar content reached a maximum at a yearly application of 174 kg K ha^–1. Averaged over years, the extractable sugar yield (BZE) increased up to the highest K application. The time of K application (autumn or spring) and the source of K (mineral fertilizer or organic material) had no effect on BZE. An additional fertilization with NaCl increased BZE only slightly in single years. Low‐grade muriate of potash containing 33% K and 3% Na can thus be used. The economically optimal K‐fertilization rate was 174 kg K ha^–1 given once in the crop rotation to sugar beet. A soil K concentration of about 110 mg (kg soil)^–1 (lactate‐extractable K) is sufficient in this soil to achieve a high BZE.  相似文献   

Chitosan based powder coating technique to enhance phytochemicals and shelf life quality of radish shreds     

《Postharvest Biology and Technology》2013

Minimally processed radish forms an important segment of the rapidly growing minimally processed industry worldwide. Radish shreds have a short shelf life thus necessitating the use of chemical additives. However, demand for natural preservatives in foods has increased. Chitosan is a natural antimicrobial biopolymer with a good film forming ability. Hence, it is used as an edible coating on whole and fresh cut fruits and vegetables. The present investigation explored powder coating technique using purified chitosan and chitosan lactate for shelf life extension of shredded radish. Macro perforated LDPE resealable pouches were used to pack the samples. Samples were stored at 10 °C for 10 d. Physicochemical characteristics, phytochemicals, antioxidant activity, respiration rate and color were analyzed periodically. The study also determined microbial load and sensory acceptability in the stored samples. Chitosan treated samples exhibited a lower degree of weight loss, respiration rate, titrable acidity, % soluble solids and higher content of phytochemicals, moisture, and pH compared with control samples. The treated samples also exhibited better sensory acceptability, lower exudate volume, lesser browning and lower microbial load compared to control. This indicated better potential marketability of the coated radish shreds. Chitosan powder coating could be used as an efficient technique for quality maintenance and shelf life extension of radish shreds with feasibility for large scale application.  相似文献   

Topical treatment of canine and feline pyoderma   总被引：1，自引：0，他引：1  

ERIC GUAGUERE 《Veterinary dermatology》1996,7(3):145-151

Abstract This paper is a review of commonly used topical antibacterial medications: benzoyl peroxide, chlorhexidine, povidone iodine, ethyl lactate, triclosan, mupirocin, neomycin, polymyxin B, bacitracin and fusidic acid. Included is a review of the pharmacokinetics, modes of action, adverse effects and clinical uses in veterinary dermatology. General recommendations for topical antibacterial therapy are presented. Résumé— Cet article est une revue des topiques antibactériens les plus couramment utilisés: peroxyde de benzoyle, chlorhexidine, povidone iodée, lactate d'éthyle, triclosan, mupirocine, néomycine, polymyxine B, bacitracine et acide fucidique. Il inclut notamment une revue des pharmacocinétiques, des modes d'action, des effets secondaires et des indications thérapeutiques de ces produits en dermatologie vétérinaire. Les indications générales du traitement topique antibactérien sont présentées. [Guaguere, E. Topical treatment of canine and feline pyoderma. (Traitement topique des pyodermites canines et félines). Veterinary Dermatology 1996; 7 : 145–151.] Resumen Este articulo es una revisión de los productos tópicos antibacterianos más frecuentemente utilizados: peróxido de benzoilo, clorhexidina, povidona yodada, etillactato, triclosan, mupirocina, neomicina, polimixina B, bacitracina y ácido fusidico. Se incluye una revisión de la farmacocinética, mecanismos de acción, efectos colaterales y sus usos clínicos en dermatologia veterinaria. Se presentan recomendaciones generales para la terapia antibacteriana tópica. [Guaguere, E. Topical treatment of canine and feline pyoderma. (Tratamiento topico de la pioderma canina y felina). Veterinary Dermatology 1996; 7 : 145–151.] Zusammenfassung— Diese Veröffentlichung besteht in einer Übersicht von häufig verwendeten topischen antibakteriellen Arzneimitteln: Benzoylperoxid, Chlorhexidin, Povidon-Jod, Ethyllaktat, Triklosan, Mupirocin, Neomycin, Polymyxin B, Bacitracin und Fusidinsäure. Mit eingeschlossen ist eine Übersicht über Pharmakokinetik, Wirkungsweise, Nebenwirkungen und klinische Anwendung in der Veterinärdermatologie. Allgemeine Empfehlungen für die lokale antibakterielle Therapie werden dargestellt. [Guaguere, E. Topical treatment of canine and feline pyoderma (Lokale Behandlung von kaninen und felinen Pyodermien). Veterinary Dermatology 1996; 7 : 145–151.]  相似文献   

四种水牛血清乳酸脱氢酶(LDH)同工酶谱型的分析研究     

栾桂龙  欧阳柱  庞廷英 《广西农业生物科学》1989,(4)

用聚丙烯酰胺凝胶圆盘电泳法对四种水牛—摩拉(Murra)、尼里(Nili-Ravi)、广西南宁本地及其三品系杂交[Ni(■× (Mu■× Na■)■]水牛血清LDH同工酶谱型进行了分析研究。结果表明,四种水牛血清LDH同工酶谱的基本特点是 LDH_1>LDH_2>LDH_3>LDH_4>LDH_5,但也出现了 LDH_2>LDH_1、LDH_3>LDH_2、LDH_4>LDH_3和LDH_5>LDH_4的情况。同时还发现四种水牛的部分个体中,除表现正常的五条酶带外,还出现一条新的酶带(LDH_6)。其血清的LDH同工酶谱,在同一品种内不同性别间的差异很小,而在不同品种间的差异则较大。  相似文献   

Effect of conditioning horses every third day at v10 on measures of fitness     

Lindner A  López RA  Durante E  Hernandez H  Botta V  Sadaba S  Boffi FM 《Journal of animal physiology and animal nutrition》2011,95(3):286-293

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Utility of the discriminant analysis to categorize untrained Spanish Pure Bred horses on the basis of biochemical and muscle variables before and after a standardized exercise test     

Escribano BM  Agüera EI  Rubio MD  Santisteban R  Vivo R  Agüera S  Tovar P 《Research in veterinary science》2011,91(3):e144-e148

We hypothesized that the information obtained from a discriminant analysis could be used to objectively discriminate horses untrained from early ages, in agreement with certain physiological characteristics.In the biopsies of 24 Spanish Pure Bred horses (1.5–3 years old) before and after a standardized exercise test (SET; 4–7 m/s with a change of velocity of 1 m/s every 2 min) muscle enzymes, substrate and metabolites were determined. Also, diverse plasma and blood parameters were considered.Three pre-exercise groups (A1: six horses; A2: seven horses and A3: eleven horses) and two post-exercise groups (B1: sixteen horses; B2: eight horses) were defined from a correspondence analysis. Forward stepwise discriminant analysis selected 11 variables which differentiated the groups between each other both pre- and post-exercise.The results of the present study suggested the utility of a discriminant analysis to categorize horses in agreement with certain physiological variables. It could be used for establishing different types of training in each group by expert trainers.  相似文献   

Protective role of propolis against reproductive toxicity of chlorpyrifos in male rats     

Reda H. ElMazoudyAzza A. Attia  Nahla S. El-Shenawy 《Pesticide biochemistry and physiology》2011,101(3):175-181

Chlorpyrifos (CPF) is causing different toxicity and propolis has been reported to be an effective antioxidant. Therefore, the present study is aimed to elucidate the possible protective effects of propolis in alleviating the toxicity of chlorpyrifos on reproductive performance, testosterone levels, enzyme activities and lipids profile in serum of male rats. Animals were divided into four groups; one orally administered (o.d.) CPF at a dose of 9 mg/kg b.w. for consecutive 70-days, propolis group (50 mg/kg b.w.), propolis and chlorpyrifos group and a control group. Results showed that there was a correlation between CPF administration and the significant decrease of the sperm counts, spermatozoon survival and testosterone level as well as increase of sperm aberrations. CPF increased significantly the lipid profile and the levels of various serum liver marker enzymes. In contrast, co-administration of propolis to CPF-treated rats restored almost most of these biochemical parameters to normal levels. On the other hand, CPF resulted in histopathological alterations in testes of male rats. However, pre-administration of propolis to CPF-treated animals improved the testicular damage and alleviates the toxic effects of CPF on reproductive functions in male rats.  相似文献   

虎奶菇菌核多糖对四氧嘧啶诱导糖尿病小鼠的降血糖作用   总被引：2，自引：0，他引：2       下载免费PDF全文

巫光宏  伍志权  何典路  蔡丽仪  曹苏杰  黄卓烈  詹福建 《华南农业大学学报》2007,28(4):100-104

通过腹腔注射四氧嘧啶建立小鼠糖尿病(DM)模型, 分别用虎奶菇菌核水提多糖(W-HNP)、碱提多糖(A-HNP)按每千克体质量200和100 mg灌胃给药,阳性对照用盐酸二甲双胍片(MH)按每千克体质量500 mg灌胃给药,连续15 d后,分别测定空腹12 h后血糖,并对小鼠血清进行乳酸脱氢酶(LDH)同工酶电泳.试验结果显示:与DM对照组相比,W-HNP、A-HNP处理组的小鼠血糖分别降低了(34.499±9.345)%、(46.820±7.032)%(P＜0.01),且不影响正常小鼠血糖;同时,多糖可改善DM小鼠的饮水、摄食和体质量,提高DM小鼠的胸腺、脾指数;DM对照组血清的LDH4、LDH5的活性比正常组高,W-HNP、A-HNP处理组的LDH4、LDH5活性比DM对照组低.  相似文献