A lactose-binding lectin from the marine sponge Cinachyrella apion (Cal) induces cell death in human cervical adenocarcinoma cells     

Rabelo L  Monteiro N  Serquiz R  Santos P  Oliveira R  Oliveira A  Rocha H  Morais AH  Uchoa A  Santos E 《Marine drugs》2012,10(4):727-743

Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5-10 μg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC(50) (10 μg/mL) for both trials and twice the IC(50) for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer.  相似文献   

Exploiting the Nephrotoxic Effects of Venom from the Sea Anemone, Phyllodiscus semoni, to Create a Hemolytic Uremic Syndrome Model in the Rat     

M Mizuno  Y Ito  BP Morgan 《Marine drugs》2012,10(7):1582-1604

In the natural world, there are many creatures with venoms that have interesting and varied activities. Although the sea anemone, a member of the phylum Coelenterata, has venom that it uses to capture and immobilise small fishes and shrimp and for protection from predators, most sea anemones are harmless to man. However, a few species are highly toxic; some have venoms containing neurotoxins, recently suggested as potential immune-modulators for therapeutic application in immune diseases. Phyllodiscus semoni is a highly toxic sea anemone; the venom has multiple effects, including lethality, hemolysis and renal injuries. We previously reported that venom extracted from Phyllodiscus semoni induced acute glomerular endothelial injuries in rats resembling hemolytic uremic syndrome (HUS), accompanied with complement dysregulation in glomeruli and suggested that the model might be useful for analyses of pathology and development of therapeutic approaches in HUS. In this mini-review, we describe in detail the venom-induced acute renal injuries in rat and summarize how the venom of Phyllodiscus semoni could have potential as a tool for analyses of complement activation and therapeutic interventions in HUS.  相似文献   

Chondrosterins A-E, triquinane-type sesquiterpenoids from soft coral-associated fungus Chondrostereum sp     

Li HJ  Xie YL  Xie ZL  Chen Y  Lam CK  Lan WJ 《Marine drugs》2012,10(3):627-638

The marine fungus Chondrostereum sp. was collected from a soft coral Sarcophyton tortuosum from the South China Sea. This fungus was cultured in potato dextrose broth medium and the culture broth was extracted with EtOAc. Five new triquinane-type sesquiterpenoids, chondrosterins A-E (1-5), and the known sesquiterpenoid hirsutanol C (6), were isolated. The structures were elucidated mainly on the basis of NMR, MS, and X-ray single-crystal diffraction data. Chondrosterin A (1) showed significant cytotoxic activities against cancer lines A549, CNE2, and LoVo with IC(50) values of 2.45, 4.95, and 5.47 μM, respectively.  相似文献   

Kiamycin, a unique cytotoxic angucyclinone derivative from a marine Streptomyces sp     

Xie Z  Liu B  Wang H  Yang S  Zhang H  Wang Y  Ji N  Qin S  Laatsch H 《Marine drugs》2012,10(3):551-558

Kiamycin (1), a new angucyclinone derivative possessing an 1,12-epoxybenz[a]anthracene ring system, was isolated from the marine Streptomyces sp. strain M268 along with the known compounds 8-O-methyltetrangomycin (3) and 8-O-methylrabelomycin (4). Their structures were elucidated by detailed spectroscopic analysis and comparison with literature data. The new angucyclinone derivative showed inhibitory activities against the human cell lines HL-60 (leukemia), A549 (lung adenocarcinoma), and BEL-7402 (hepatoma) with inhibition rates of 68.2%, 55.9%, and 31.7%, respectively, at 100 μM. It appears to have potential as an anticancer agent with selective activity.  相似文献   

Sesquiterpene and Acetogenin Derivatives from the Marine Red Alga Laurencia okamurai     

Yi Liang  Xiao-Ming Li  Chuan-Ming Cui  Chun-Shun Li  Hong Sun  Bin-Gui Wang 《Marine drugs》2012,10(12):2817-2825

In addition to 13 known compounds, four new bisabolane sesquiterpenes, okamurenes A–D (1–4), a new chamigrane derivative, okamurene E (5), and a new C₁₂-acetogenin, okamuragenin (6), were isolated from the marine red alga Laurencia okamurai. The structures of these compounds were determined through detailed spectroscopic analyses. Of these, okamurenes A and B (1 and 2) are the first examples of bromobisabolane sesquiterpenes possessing a phenyl moiety among Laurencia-derived sesquiterpenes, while okamuragenin (6) was the first acetogenin aldehyde possessing a C₁₂-carbon skeleton. Each of the isolated compounds was evaluated for the brine shrimp (Artemia salina) lethal assay and 7-hydroxylaurene displayed potent lethality with LD₅₀ 1.8 μM.  相似文献   

Meroterpenes from Endophytic Fungus A1 of Mangrove Plant Scyphiphora hydrophyllacea     

Wen-Li Mei  Bo Zheng  You-Xing Zhao  Hui-Ming Zhong  Xun-Li Wu Chen  Yan-Bo Zeng  Wen-Hua Dong  Jiu-Li Huang  Peter Proksch  Hao-Fu Dai 《Marine drugs》2012,10(9):1993-2001

Four new meroterpenes, guignardones F–I (1–4), together with two known compounds guignardones A (5) and B (6) were isolated from the endophytic fungus A1 of the mangrove plant Scyphiphora hydrophyllacea. Their structures and relative configurations were elucidated by spectroscopic data and single-crystal X-ray crystallography. A possible biogenetic pathway of compounds 1–6 was also proposed. All compounds were evaluated for inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus.  相似文献   

船用柴油机故障诊断系统研究     

朱发新  蔡振雄  卢金树  王家宏  张志斌 《浙江水产学院学报》2011,(1):61-65

船用柴油机故障诊断系统可以提高轮机工作人员的管理水平,达到快速分析、准确判断及迅速排除故障。它以船用柴油机典型故障为基础,应用Visual FoxPro数据库管理系统建立船用柴油机故障诊断系统。通过柴油机拉缸故障典型实例来具体说明该系统的使用方法和特点。该系统具有可修改、插入和删除等功能,以友好的用户界面和易于操作等特点,实现船用柴油机典型故障的快速诊断。该文的创新点是将船员具有的故障处理的经验及知识作为专家系统,供其他船员的学习,以便准确、快速地处理柴油机的故障。  相似文献   

中国重要海洋中上层经济鱼类生物学研究进展   总被引：21，自引：3，他引：18  

郑元甲  李建生  张其永  洪万树 《水产学报》2014,38(1):149-160

中国海洋中上层鱼类的种类繁多,近几年来,其总产量占全国海洋捕捞总产量的40%左右。日本鲭、蓝圆鲹和鳀等中上层鱼类生活史大多为r选择型,资源更新速度一般比底层和近底层鱼类较快,因此其资源可持续利用的前景更为广阔。本文综述了中国重要海洋中上层鱼类渔业的发展概况,主要介绍了年产量位于前列的日本鲭、蓝圆鲹、蓝点马鲛、银鲳和鳀等5种鱼类生物学的研究进展,阐明了这些种类的种群鉴别和划分、洄游分布、越冬场、产卵场和产卵期、年龄和生长、摄食习性、生殖力和群体结构变化等资源生物学的研究现状,以及其资源量和可捕量的评估结果,并展望了其资源生物学研究的前景。  相似文献   

Lindgomycin,an Unusual Antibiotic Polyketide from a Marine Fungus of the Lindgomycetaceae     

Bin Wu  Jutta Wiese  Antje Labes  Annemarie Kramer  Rolf Schmaljohann  Johannes F. Imhoff 《Marine drugs》2015,13(8):4617-4632

An unusual polyketide with a new carbon skeleton, lindgomycin (1), and the recently described ascosetin (2) were extracted from mycelia and culture broth of different Lindgomycetaceae strains, which were isolated from a sponge of the Kiel Fjord in the Baltic Sea (Germany) and from the Antarctic. Their structures were established by spectroscopic means. In the new polyketide, two distinct domains, a bicyclic hydrocarbon and a tetramic acid, are connected by a bridging carbonyl. The tetramic acid substructure of compound 1 was proved to possess a unique 5-benzylpyrrolidine-2,4-dione unit. The combination of 5-benzylpyrrolidine-2,4-dione of compound 1 in its tetramic acid half and 3-methylbut-3-enoic acid pendant in its decalin half allow the assignment of a new carbon skeleton. The new compound 1 and ascosetin showed antibiotic activities with IC₅₀ value of 5.1 (±0.2) µM and 3.2 (±0.4) μM, respectively, against methicillin-resistant Staphylococcus aureus.  相似文献   

Marine Carotenoids against Oxidative Stress: Effects on Human Health     

Maria Alessandra Gammone  Graziano Riccioni  Nicolantonio D’Orazio 《Marine drugs》2015,13(10):6226-6246

Carotenoids are lipid-soluble pigments that are produced in some plants, algae, fungi, and bacterial species, which accounts for their orange and yellow hues. Carotenoids are powerful antioxidants thanks to their ability to quench singlet oxygen, to be oxidized, to be isomerized, and to scavenge free radicals, which plays a crucial role in the etiology of several diseases. Unusual marine environments are associated with a great chemical diversity, resulting in novel bioactive molecules. Thus, marine organisms may represent an important source of novel biologically active substances for the development of therapeutics. In this respect, various novel marine carotenoids have recently been isolated from marine organisms and displayed several utilizations as nutraceuticals and pharmaceuticals. Marine carotenoids (astaxanthin, fucoxanthin, β-carotene, lutein but also the rare siphonaxanthin, sioxanthin, and myxol) have recently shown antioxidant properties in reducing oxidative stress markers. This review aims to describe the role of marine carotenoids against oxidative stress and their potential applications in preventing and treating inflammatory diseases.  相似文献