首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   3205篇
  免费   200篇
  国内免费   430篇
林业   187篇
农学   370篇
基础科学   316篇
  594篇
综合类   1347篇
农作物   107篇
水产渔业   116篇
畜牧兽医   684篇
园艺   23篇
植物保护   91篇
  2024年   29篇
  2023年   74篇
  2022年   104篇
  2021年   106篇
  2020年   131篇
  2019年   134篇
  2018年   85篇
  2017年   127篇
  2016年   159篇
  2015年   135篇
  2014年   168篇
  2013年   192篇
  2012年   216篇
  2011年   232篇
  2010年   192篇
  2009年   166篇
  2008年   164篇
  2007年   208篇
  2006年   197篇
  2005年   138篇
  2004年   123篇
  2003年   99篇
  2002年   71篇
  2001年   67篇
  2000年   53篇
  1999年   71篇
  1998年   48篇
  1997年   39篇
  1996年   41篇
  1995年   32篇
  1994年   36篇
  1993年   47篇
  1992年   38篇
  1991年   25篇
  1990年   12篇
  1989年   21篇
  1988年   15篇
  1987年   6篇
  1986年   2篇
  1985年   3篇
  1984年   5篇
  1982年   3篇
  1980年   5篇
  1979年   2篇
  1978年   2篇
  1977年   2篇
  1975年   1篇
  1962年   1篇
  1955年   8篇
排序方式: 共有3835条查询结果,搜索用时 203 毫秒
101.
为完善湿垫风机系统在农业建筑夏季蒸发冷却中的应用,研究了湿垫冷却装置在喷淋水完全蒸发条件下的热质交换特征,经理论推导给出了确定喷淋水量及湿垫合理参数的计算式。  相似文献   
102.
Fatty acid profiles of the total lipids and the phospholipid classes of farm-raised channel catfish (Ictalurus punctatus) fillets were analyzed. Monoenes represented 49.22 mol% of the total lipid fatty acids while polyunstaturated fatty acids (PUFA) accounted for 19.37 mol%. The total n-3 PUFA content was low (4.22 mol%). The total saturated fatty acids as 31.44 mol %. Catfish contained phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), lysophosphatidylcholine (LPC), and sphingomyelin (SPH). Analysis for the individual phospholipid class fatty acid profiles indicated that PI had a high concentration of 18:0 (33.20 mol%) and 20:4 n-6 (13.08 mol%). Phosphatidylethanolamine had the highest concentrations of combined eicosapentaenoic acid, and docoshahexaenoic acid, of 17.37 mol%. Higher ratios of n-3/n-6 PUFA were found in the phospholipid classes than the total lipids. LPC and SPH did not contain measurable PUFA of the n-3 series. The level of 18:1 n-9 in PC, PE, and LPC was approximately 31 mol% whereas in PI, PS, and SPH, the level was approximately 16 mol%. The understanding of the basic distribution of fatty acids in the individual phospholipid classes of channel catfish may be an essential first step in explaining the protective role of 18:1 n-9 and n-3 PUFA against thrombotic and cardiovascular disorder in humans.  相似文献   
103.
通过冷冻胚胎移植技术,2007年和2008年前后分2批在鄂尔多斯细毛羊种羊场实施纯种澳美型细毛羊冷冻胚胎移植,共有371只(2007年266只,2008年105只)受体羊做了冷冻胚胎移植,共生产澳美型细毛羊羔羊106只(2007年70只,2008年36只)。其中公羔60只,母羔46只;受胎率分别为26.32%和37.14%;羔羊初生重为公羔(4.73±0.41)kg/只、母羔(4.54±0.43)kg/只和公羔(4.60±0.68)kg/只、母羔(4.45±0.55)kg/只。经过调查鉴定,用冷冻胚移植技术获得羔羊106只,经培养共获得纯种澳美型细毛羊106只,补充了优质澳美型细毛羊种羊的数量,大大增加了纯种澳美型细毛羊的种源数量、节省了引种费用、缩短了鄂尔多斯细毛羊改良世代间隔和选育提高周期,起到了加速优良种畜繁殖速度、迅速扩大种源的科技效应。  相似文献   
104.
“母语语音负迁移”是中国学生在英语学习中普遍遇到的问题,其消极影响是显而易见的。本文从探讨汉语拼音的发音方法对英语语音的负迁移现象入手,揭示英汉语音的相似性与差异性。对英汉语音相似性与差异性的认知能促使中国学生在说英语时有意识地进行自我纠音,因此笔者认为在语音教学实践中引入英汉语音对比的相关知识及其研究成果将能有效地降低中国学生母语语音对目的语的干扰作用,从而提高语音教学质量。  相似文献   
105.
研究可编程系统芯片(SOPC)设计中常见的多时钟域间的数据传递。分析多时钟域间亚稳态的产生机理和危害,并讨论消除亚稳态的参数约束和多时钟域数据传递的不同形式。针对常用的同步化策略,提出六种数据可靠传递的方法,并简析其应用特点。  相似文献   
106.
指出了木材干燥窑壳体传热量的传统计算方法中的不足,并利用形状因子法来分析计算木材干燥窑壳体的传热量.对比2种计算方法所得的结果表明,传统的干燥窑壳体计算方法较为保守,而根据形状因子法计算结果来配置干燥窑的加热器面积则不会造成干燥窑中散热设备投资的浪费,同时也可以节省干燥窑墙体设计用材的量.  相似文献   
107.
采用雷诺应力模型和Cross粘度方程,对二维轴对称圆管内湍流流动的聚丙烯酰胺稀溶液(PAM)的流场和温度场进行了数值模拟.在数值计算结果的基础上,系统研究了流速对减阻流体的减阻性能和传热特性的影响.结果表明,减阻流体的减阻率和传热降低率随流速的增大逐渐增大到渐进值,而且传热降低率恒大于减阻率.减阻流体柯尔本(Colburn)因子与摩擦系数的对比结果表明柯尔本比拟,特别是在较低流动雷诺数下有较大偏差.  相似文献   
108.
Objective To determine the regional incidence and effectiveness of treatment of failure of passive transfer (FPT) in foals. Design A study of disease incidence. Animals Eighty-eight foals and 57 mares from four studs in the practice area of the Rural Veterinary Centre were tested. Procedure Foals were tested for their serum IgG and total serum protein (TSP) concentration within the first 72 hours of life. Colostrum was collected from mares and specific gravity determined. FPT and partial failure of passive transfer (PFPT) of immunoglobulins was diagnosed when serum IgG concentrations were < 4 g/L and 4 to 8 g/L respectively. Owners of foals diagnosed with FPT were offered treatment with 1 to 2 L plasma (TSP > 70 g/L); 9 (64%) of the affected foals were treated. Results Fourteen foals (16%) had FPT whereas 15 (17%) had PFPT. There were significant differences between the mean TSP concentration in foals with FPT (42.6 ± 4.2 g/L), PFPT (48.1 ± 3.9 g/L) and those acquiring adequate passive immunity (58.9 ± 5.5 g/L) (P < 0.01). Sixteen (29%) mares had pre-suck colostral specific gravity < 1.060 and 12 (71%) foals raised by these mares had FPT or PFPT. The incidence of severe disease (categorised by a sepsis score > 11, positive culture of bacteria from blood or disease requiring hospitalisation) in all foals in the first 2 months of life was 10%. However, none of the nine foals with FPT that received plasma experienced severe disease. In contrast, foals with PFPT had an increased susceptibility to severe disease (P < 0.001) when compared with normal foals. Conclusion Treatment of foals with FPT may reduce the subsequent incidence of severe disease. Pre-suck colostral specific gravity and foal TSP may be used to predict the likelihood of FPT and PFPT. Even though the number of foals studied is small the results highlight the importance of optimal management practices in reducing the incidence of FPT and disease associated with this process.  相似文献   
109.
In vivo matured oocytes collected by laparoscopic follicular aspiration (LFA) from hormone treated female goats were used as recipient ooplasts for somatic cell nuclear transfer (SCNT). Japanese native (Shiba) goats were used as donor females and some donor females were used repeatedly (two or three times) at intervals of a few months. To induce synchronization of estrus, a sponge containing 0.5 g of progesterone was inserted into the vagina of each goat for 14 days. These animals were also treated with follicle stimulating hormone (FSH) in a series of 8 injections over 4 days. The first FSH injection was administered on the morning of day 9 of sponge insertion. On the morning of day 13, 50 µg of gonadotropin‐releasing hormone (GnRH) was injected into each animal. Twenty‐nine hours after GnRH injection, LFA was performed. After removal of cumulus cells, collected oocytes with the first polar body were selected and enucleated for nuclear transfer. Anterior pituitary cells isolated from an adult male Shiba goat were transfected with a DNA fragment containing the enhanced green flourescent protein gene and the puromycin resistance gene. A single donor cell was inserted into the perivitelline space of each enucleated oocyte and fusion was induced with one electric pulse of 20 V for 10 µs. The SCNT goat eggs were cultured in chemically defined medium at 38.5°C in 5% CO2, 5% O2, 90% N2 for 9 days. By LFA, 396 oocytes were collected from a total of 30 females. After removal of cumulus cells, 64% of them extruded the first polar body. The percentage of SCNT goat eggs produced using in vivo matured oocytes which developed to the blastocyst stage (20–21%) was significantly higher (P < 0.05) than that produced with in vitro matured oocytes (3–8%).  相似文献   
110.
【目的】探究不同妊娠时期猪胎盘的氨基酸、葡萄糖、脂肪酸转运体的表达模式。【方法】选择15头遗传背景、产仔数接近的杜洛克2~4胎经产健康母猪平均分为3组,所有母猪发情后使用相同公猪精液进行人工授精,在妊娠第40天(D40)、65天(D65)和95天(D95)通过麻醉分别取出每组母猪子宫,快速打开子宫分离出每个胎儿的胎盘组织,提取胎盘组织总RNA并反转录合成cDNA,利用合成的引物进行普通PCR扩增,用2.0%琼脂糖凝胶检测扩增产物。采用实时荧光定量PCR检测并比较3个时期胎盘中氨基酸、葡萄糖、脂肪酸转运体相关基因mRNA相对表达水平。【结果】PCR检测结果显示,氨基酸转运体相关基因(SLC7A1、SLC7A2、SLC7A3、SLC7A4、SLC7A10、SLC1A3、SLC1A5、SLC38A10、SLC36A1)、葡萄糖转运体相关基因(SLC2A1、SLC2A2、SLC2A3、SLC2A10、SLC2A12、SLC2A13)及脂肪酸转运体相关基因(FATP1、FATP2、FATP3、FATP4、FABP3、FABP5、FABP7、CD36)的片段长度均与预期相符。实时荧光定量PCR结果显示,在氨基酸转运体中,D65胎盘中SLC7A4、SLC7A10、SLC38A10基因表达水平显著高于D40胎盘(P<0.05),而SLC7A2基因表达水平显著低于D40胎盘(P<0.05),且D65胎盘的SLC1A3和SLC7A4基因表达水平均显著低于D95胎盘(P<0.05);在葡萄糖转运体中,D65和D95胎盘的SLC2A3和SLC2A13基因表达水平显著高于D40胎盘(P<0.05),D95胎盘的SLC2A1、SLC2A2和SLC2A12基因表达水平显著低于D65胎盘(P<0.05);在脂肪酸转运体中,D65胎盘的FATP2、FATP4、FABP3、FABP5、FABP7和CD36基因表达水平显著高于D40胎盘(P<0.05),而FATP1、FATP4和CD36基因表达水平显著低于D95胎盘(P<0.05)。【结论】在猪妊娠过程中,胎盘中SLC7A10、SLC38A10、SLC7A4、SLC2A3、FATP1、FATP4、FABP5、CD36等基因可能是影响胎儿生长发育的重要营养转运基因。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号