Molting drives mortality in foraging populations of Formosan subterranean termites (Isoptera: Rhinotermitidae) baited with a chitin synthesis inhibitor,noviflumuron          下载免费PDF全文

Garima Kakkar  Nan‐Yao Su 《Pest management science》2018,74(1):219-224

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范伏元教授治疗结缔组织相关性肺间质病变经验          下载免费PDF全文

凌嫘  范伏元 《勤云标准版测试》2019,39(7):867-869

范伏元教授认为结缔组织相关性肺间质病变治疗需分三期论治：初期治以疏风宣肺、止咳化痰;中期治以清热化痰、活血化瘀;晚期治以补脾肺肾、理气通络。临床运用疏风宣肺汤、柴芩温胆汤、归芪补肺汤等方临证加减治疗结缔组织相关性肺间质病变获满易疗效。  相似文献   

Detection of neoplastic lymphocytes in peripheral blood of dogs with lymphoma by polymerase chain reaction for antigen receptor gene rearrangement     

Keller RL  Avery AC  Burnett RC  Walton JA  Olver CS 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(3):145-149

BACKGROUND: Uniquely rearranged immunoglobulin and T-cell receptor gene sequences can be amplified and electrophoretically separated by size to detect a clonal population of lymphocytes. OBJECTIVE: The purpose of this study was to determine whether the polymerase chain reaction (PCR) detects neoplastic (clonal) lymphocytes more frequently than do microscopic methods. METHODS: We identified neoplastic lymphocytes in peripheral blood by both routine and standardized microscopic examination of blood smears and by PCR amplification of blood-derived DNA and compared the 3 methods for frequency of detection of leukemic involvement. For standardized microscopic examination (200 leukocytes counted on Wright-Giemsa-stained blood smears), samples were categorized as negative (1% prolymphocytes, no lymphoblasts), or positive (>/=1 lymphoblast). A PCR-amplified sample was positive if 1 or 2 discrete bands were seen on the gel, or negative if no bands, a smear, or a faint ladder was seen. RESULTS: Using PCR, neoplastic lymphocytes were detected in peripheral blood 2.5 times more frequently than with routine or standardized microscopic evaluation. Eighty-three percent of samples negative by microscopy were positive by PCR. CONCLUSION: PCR is more sensitive than microscopy for the detection of clonal lymphocytes in peripheral blood. The results of this study also suggest that neoplastic lymphocytes circulate in peripheral blood at a higher frequency than previously reported. PCR may be useful for detecting or phenotyping lymphoma, monitoring response to therapy, identifying recurrence, and screening breeds at risk.  相似文献   

Computed tomographic‐lymphography as a complementary technique for lymph node staging in dogs with malignant tumors of various sites          下载免费PDF全文

Maximilian Körner  José Suárez  Gregorio Carozzi  Valeria S. Meier  Malgorzata Roos  Carla Rohrer Bley 《Veterinary radiology & ultrasound》2018,59(2):155-162

Locoregional lymph nodes are routinely examined in order to define the spatial extent of neoplastic disease. As draining patterns of certain tumor types can be divergent from expected anatomical distribution, it is critical to sample the lymph nodes truly representing the draining area. The aim of this bicenter prospective pilot study was to describe the technique of computed tomographic (CT)‐lymphography for primary draining lymph node mapping in tumor staging in dogs. Forty‐five dogs with macro‐ or microscopic tumors in specified localizations were evaluated. Depending on body weight, 0.8–2 ml contrast agent (iohexol) was injected into four quadrants around the tumor, and CT‐images were obtained at 1, 3, 6, 9, and 12 minutes post‐injection. Attenuation of chosen regions of interest (Hounsfield units (HU)) and patterns of enhancement were assessed for 284 lymph nodes in the precontrast study with median HUs of 31.1 (Interquartile range (IQR) = 18.4) and for 275 in the intravenous postcontrast study with 104.3 HU (IQR = 31.2) (paired Wilcoxon test, P < 0.001). In the CT‐lymphography study, 45 primary draining lymph nodes with a significantly higher median HU value of 348.5 (IQR = 591.4) (one‐sample t‐test, P < 0.001) were identified. Primary draining lymph nodes were found to be clearly visible after 1–3 minutes after local injection, often concurrent with a good visibility of the lymphatic vessel system. The herein described technique of peritumorally injected CT‐contrast agent followed by subsequent CT‐lymphography for primary draining lymph node mapping works well in a majority of cases in all investigated sites and warrants further validation for different tumor entities.  相似文献   

Microbiological Impact of Delayed Movement of Pullets     

《The Journal of Applied Poultry Research》2019,28(2):318-328

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Survival analysis in dogs with urinary transitional cell carcinoma that underwent whole‐body computed tomography at diagnosis     

Ryota Iwasaki  Yuka Shimosato  Ryutaro Yoshikawa  Sho Goto  Kyoko Yoshida  Mami Murakami  Mifumi Kawabe  Hiroki Sakai  Takashi Mori 《Veterinary and comparative oncology》2019,17(3):385-393

This retrospective study aimed to evaluate factors associated with survival and to compare characteristics between tumour localizations in dogs with urinary transitional cell carcinoma (TCC) that underwent whole‐body computed tomography (CT) at diagnosis. Dogs with histologically confirmed TCC that received medical therapy between 2010 and 2017 were included; dogs that underwent surgery or radiotherapy for the primary tumour were excluded. According to the CT findings, primary tumour localization (classified into the Bladder, Urethra and Bladder and Urethra groups), prostate involvement, iliosacral lymphadenomegaly, sternal lymphadenomegaly and metastasis to the bone and lung were evaluated for survival analysis. CT at diagnosis revealed iliosacral lymphadenomegaly, sternal lymphadenomegaly, bone metastasis and lung metastasis in 47.7%, 18.5%, 24.6% and 35.4% of the 65 included dogs, respectively. The overall median survival time was 196 days. On multivariable analysis, TCC localization (hazard ratio [HR], 1.90; P = .037), bone metastasis (HR, 2.76; P = .013) and sternal lymphadenomegaly (HR, 3.56; P = .004) were significantly associated with survival. Compared to the Bladder group (n = 16), the Urethra group (n = 26) had higher metastasis rates to the bone (6.3% vs 42.3%; P = .045) and lung (6.3% vs 46.2%; P = .022). The survival time was shorter in the Urethra group than in the Bladder group (121.5 vs 420 days; P < .001), and it was similar only in female dogs (247 vs 420 days; P = .031). These findings suggest that whole‐body CT could be valuable for predicting the prognosis in urinary TCC.  相似文献   

Load-carrying Capacity Calculate and Analysis of Staging Stake     

YAO Gang  LIU Wei-liang  ZHOU Zhong-ming Co. t  Chongqing  Chin 《保鲜与加工》2006,(9):134-137

Falsework is a kind of scaffoldwork using load bearing. It has widely use in scaffoldwork and bridgework and steel construction. For assuring falsework safety on structure construction, it is very important to quickly and accurately calculate falsework. Load - carrying capacity of staging of should be calculate by special design, which is from common used single row or double rows falsework. The authors analyze all kinds of factor and failure mode related to carrying capacity and find the result of numerical list by ANSYS program and code when the parameter of staging is determinated. It has important gaidance role in erection of falsework pole.  相似文献   

Hemolymph vitellogenin levels and ovarian development during the reproductive and non-reproductive molt cycles in the giant freshwater prawn Macrobrachium rosenbergii   总被引：2，自引：0，他引：2  

Takuji Okumura  Katsumi Aida 《Fisheries Science》2000,66(4):678-685

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Induction of spawning in Pacific white shrimp Litopenaeus vannamei (Boone, 1931) by injection of its molt inhibiting hormone isoform II produced in E. coli     

Mayte Gonzlez  Jesús Luis Betancourt  Tania Rodríguez‐Ramos  Mario Pablo Estrada  Yamila Carpio  Laida Ramos 《Aquaculture Research》2020,51(8):3100-3108

The optimization of reproductive parameters in shrimp farming continues to be a challenge for most producing countries. Although the crustacean neuropeptides have been studied extensively in the last two decades, the functions of most of these neuropeptides remained putative. Among them, molt‐inhibiting hormone isoform II (MIH II) has shown an important role in vitellogenesis. In this study, the cDNA encoding mature MIH II peptide was isolated by RT‐PCR from the L. vannamei eyestalk. The cDNA was cloned into pET28a bacterial expression vector. Recombinant MIH II was obtained in the form of insoluble inclusion bodies and purified to ~88% purity. Two doses of rMIH II and a negative control group were assayed in vivo. The stages of ovarian maturation and spawning were recorded during 72 hr post‐injection. The results showed that ovarian maturation occurred approximately in 9% and 33% of females injected with rMIH II at the doses of 300 and 600 ng/gbw respectively. Neither maturation nor spawning was detected in the negative control group. Females injected with 600 ng/gbw, which showed vitellogenic stages III and IV, spawned. These preliminary results argue that the hormone rMIH II could be a promising candidate to induce spawning in L. vannamei shrimp.  相似文献   

Investigation of vaginal mucus parameters: Development of models for staging the oestrous cycle of the Bunaji cow     

Linda Chinyere Agbugba  Adetomi Oyelola Oyewunmi  Tayo Peter Ogundumade  Olufisayo Oluwadamilare Leigh 《Reproduction in domestic animals》2020,55(9):1044-1053

Changes in some vaginal mucus parameters were studied in order to generate predictive models capable of enhancing oestrous cycle staging, using equal groups (unsynchronized-USC [no treatment] and synchronized-SC [Synchromate^® i/m on d0, d11]) of Bunaji cows (n = 48) aged 3–4 years. Vaginal mucus was collected (starting d11 in SC) daily over 26 days using standard procedures. Physical (viscosity, elasticity, density, resistivity) and biochemical (pH, glucose, cholesterol, total protein, calcium, magnesium, sodium, potassium) parameters were evaluated using standard procedures. Data were analysed using chi-square and multinomial logit regression modelling. Models generated using oestrus as reference categories were ascertained for accuracies. Chi-square values for viscosity, elasticity and density were significant (p < .01) in USC and SC across stages of the cycle. Results for USC showed that pH and cholesterol were predictive (p < .01) for pro-oestrus, metoestrus and dioestrus, while total protein was predictive (p < .01) for dioestrus only. Similarly, magnesium was predictive (p < .05) for pro-oestrus. For SC, pH, magnesium and cholesterol were predictive (p < .01) for pro-oestrus, metoestrus and dioestrus, while total protein was predictive (p < .01) for pro-oestrus and dioestrus. Potassium and total protein were also predictive for metoestrus at 10% and 5% significance levels, respectively. Though findings suggest the usefulness of magnesium in staging the oestrous cycle only in synchronized cows, pH, total protein and cholesterol appeared to be the more important vaginal mucus parameters in Bunaji cows, regardless synchronization. Furthermore, the models developed showed high accuracy levels for staging the oestrous cycle in USC (100%) and SC (89%).  相似文献