城市绿化中大树反季节移植技术   总被引：8，自引：0，他引：8  

王振一 《河北林果研究》2006,21(3):354-356

阐述了大树反季节移植在城市绿化中的重要作用,以及影响大树移植成活的原因。提出了提高大树反季节移植成活率的技术措施。  相似文献   

成年金丝小枣树移植及管理技术     

李行军  陈彦华  张玉芳  哈丽娟  刘长柏  李同芹 《河北林果研究》2006,21(1):69-70

为提高经济效益,减少损失,对金丝小枣成年大树移植技术进行了研究。论述了移植前的各项准备工作,移栽技术及移栽后的各项管理措施。  相似文献   

四种限制性内切酶对美国白蛾核型多角体病毒DNA酶解分析          下载免费PDF全文

韩一侬  杨玲  于在林 《林业科学研究》1990,3(4):341-344

从HcNPV中直接分离提纯的DNA证明具有典型的紫外吸收光谱。经琼脂糖电泳后是一条带谱。用限制性内切酶EcoRⅠ、PstⅠ、BglⅡ、PVUⅡ酶解,经琼脂糖平板电泳分别产生19、22、18、22条核酸片段。以λDNA-EcoRⅠ和λDNA-HindⅢ片段作参考,计算出各片段分子量大小,积加得平均值为94.0445×10~8。  相似文献   

Characterization of an intravenous lipopolysaccharide inflammation model in calves with respect to the acute-phase response     

《Veterinary immunology and immunopathology》2015,163(1-2):46-56

Our objective was to develop a lipopolysaccharide (LPS) inflammation model in calves to evaluate the acute-phase response with respect to the release of pro-inflammatory cytokines and acute-phase proteins, fever development and sickness behaviour. Fourteen 4-week-old male Holstein Friesian calves were included and randomly assigned to a negative control group (n = 3) and an LPS-challenged group (n = 11). The latter received an intravenous bolus injection of 0.5 μg of LPS/kg body weight. Blood collection and clinical scoring were performed at 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6, 8, 12, 18, 24, 28, 32, 48, 54 and 72 h post LPS administration (p.a.). In the LPS group, the following clinical signs were observed successively: tachypnoea (on average 18 min p.a.), decubitus (29 min p.a.), general depression (1.75 h p.a.), fever (5 h p.a.) and tachycardia (5 h p.a.). Subsequent to the recovery from respiratory distress, general depression was prominent, which deteriorated when fever increased. One animal did not survive LPS administration, whereas the other animals recovered on average within 6.1 h p.a. Moreover, the challenge significantly increased plasma concentrations of tumour necrosis factor-α, interleukin 6, serum amyloid A and haptoglobin, with peaking levels at 1, 3.5, 24 and 18 h p.a., respectively. The present LPS model was practical and reproducible, caused obvious clinical signs related to endotoxemia and a marked change in the studied inflammatory mediators, making it a suitable model to study the immunomodulatory properties of drugs in future research.  相似文献   

Propofol attenuates LPS-induced tumor necrosis factor-α, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-κB activation     

Zengyang PEI  Jinqiu WANG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):139-145

Sepsis is a major cause of mortality in intensive care medicine. Propofol, an intravenous general anesthetic, has been suggested to have anti-inflammatory properties and able to prevent sepsis induced by Gram-positive and Gram-negative bacteria by down-regulating the gene expression of pro-inflammatory cytokines. However, propofol’s anti-inflammatory effects upon canine peripheral blood mononuclear cells (PBMCs) have not yet been clarified. Here, we isolate canine PBMCs and investigate the effects of propofol on the gene expressions of both lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α and upon the production of nitric oxide (NO). Through real-time quantitative PCR and the Griess reagent system, we found that non-cytotoxic levels of propofol significantly inhibited the release of NO and IL-6 and TNF-α gene expression in LPS-induced canine PBMCs. Western blotting revealed that LPS does significantly increase the expression of inducible NO synthase (iNOS) protein in canine PBMCs, while pretreatment with propofol significantly decreases the LPS-induced iNOS protein expression. Propofol, at concentration of 25 µM and 50 µM, also significantly inhibited the LPS-induced nuclear translocation of nuclear factor (NF)-κB p65 protein in canine PBMCs. This diminished TNF-α, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-κB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 µM and 50 µM propofol. This suggests that non-cytotoxic levels of propofol pretreatment can down-regulate LPS-induced inflammatory responses in canine PBMCs, possibly by inhibiting the nuclear translocation of the NF-κB p65 protein.  相似文献   

Cell-free extract from porcine induced pluripotent stem cells can affect porcine somatic cell nuclear reprogramming     

Jin-Gu NO  Mi-Kyung CHOI  Dae-Jin KWON  Jae Gyu YOO  Byoung-Chul YANG  Jin-Ki PARK  Dong-Hoon KIM 《The Journal of reproduction and development》2015,61(2):90-98

Pretreatment of somatic cells with undifferentiated cell extracts, such as embryonic stem cells and mammalian oocytes, is an attractive alternative method for reprogramming control. The properties of induced pluripotent stem cells (iPSCs) are similar to those of embryonic stem cells; however, no studies have reported somatic cell nuclear reprogramming using iPSC extracts. Therefore, this study aimed to evaluate the effects of porcine iPSC extracts treatment on porcine ear fibroblasts and early development of porcine cloned embryos produced from porcine ear skin fibroblasts pretreated with the porcine iPSC extracts. The Chariot^TM reagent system was used to deliver the iPSC extracts into cultured porcine ear skin fibroblasts. The iPSC extracts-treated cells (iPSC-treated cells) were cultured for 3 days and used for analyzing histone modification and somatic cell nuclear transfer. Compared to the results for nontreated cells, the trimethylation status of histone H3 lysine residue 9 (H3K9) in the iPSC-treated cells significantly decreased. The expression of Jmjd2b, the H3K9 trimethylation-specific demethylase gene, significantly increased in the iPSC-treated cells; conversely, the expression of the proapoptotic genes, Bax and p53, significantly decreased. When the iPSC-treated cells were transferred into enucleated porcine oocytes, no differences were observed in blastocyst development and total cell number in blastocysts compared with the results for control cells. However, H3K9 trimethylation of pronuclear-stage-cloned embryos significantly decreased in the iPSC-treated cells. Additionally, Bax and p53 gene expression in the blastocysts was significantly lower in iPSC-treated cells than in control cells. To our knowledge, this study is the first to show that an extracts of porcine iPSCs can affect histone modification and gene expression in porcine ear skin fibroblasts and cloned embryos.  相似文献   

Quantitative morphology in canine cutaneous soft tissue sarcomas          下载免费PDF全文

R. Simeonov  J. Ananiev  M. Gulubova 《Veterinary and comparative oncology》2015,13(4):481-484

Stained cytological specimens from 24 dogs with spontaneous soft tissue sarcomas [fibrosarcoma (n = 8), liposarcoma (n = 8) and haemangiopericytoma (n = 8)], and 24 dogs with reactive connective tissue lesions [granulation tissue (n = 12) and dermal fibrosis (n = 12)] were analysed by computer‐assisted nuclear morphometry. The studied morphometric parameters were: mean nuclear area (MNA; µm²), mean nuclear perimeter (MNP; µm), mean nuclear diameter (MND mean; µm), minimum nuclear diameter (D_min; µm) and maximum nuclear diameter (D_max; µm). The study aimed to evaluate (1) possibility for quantitative differentiation of soft tissue sarcomas from reactive connective tissue lesions and (2) by using cytomorphometry, to differentiate the various histopathological soft tissue sarcomas subtypes in dogs. The mean values of all nuclear cytomorphometric parameters (except for D_max) were statistically significantly higher in reactive connective tissue processes than in soft tissue sarcomas. At the same time, however, there were no considerable differences among the different sarcoma subtypes. The results demonstrated that the quantitative differentiation of reactive connective tissue processes from soft tissue sarcomas in dogs is possible, but the same was not true for the different canine soft tissue sarcoma subtypes. Further investigations on this topic are necessary for thorough explication of the role of quantitative morphology in the diagnostics of mesenchymal neoplasms and tumour‐like fibrous lesions in dogs  相似文献   

抗流感病毒药物研究进展     

罗小琴  吴德峰 《家畜生态学报》2015,36(3):86-90

季节性和爆发性流感引起了高发病率和死亡率,不仅严重危害人体健康,还影响社会稳定和经济发展。目前对抗流感的主要措施是预防接种,然而流感病毒变异速度非常快,使得现有的疫苗对新变异的流感病毒无效或者预防效果不好。因此,抗病毒药物的研究显得非常必要,而被深入研究的流感病毒的生命周期和病毒结构蛋白也推进了抗流感病毒药物设计的进展。本文将对抗流感药物设计靶向进行概述,旨在为今后研究开发出更高效、更理想的多靶向抗流感病毒药物提供借鉴。  相似文献   

激光切割对马铃薯组培苗移栽后生长指标的影响     

徐涛  段宏兵  蔡兴奎  杨锐  姚飞虎  严福勇 《华中农业大学学报》2021,40(4):80-84

以机械剪切方式作为对照,使用以CO_2激光器在垂直照射、离焦量12 mm、光输出功率10 W的条件下的激光,对马铃薯组培苗进行无接触式切割,并对激光切割和机械剪切后马铃薯组培苗的生长情况进行对比试验。试验结果显示:使用激光进行马铃薯组培苗切割不会影响其生物活性;在培养21 d后,无论是单切口茎段还是双切口茎段,激光切割马铃薯组培苗的存活率、株高、节间数、节间长度、茎粗、根长、去根鲜质量等生长指标均优于机械剪切方式。试验结果表明,以激光切割方式代替传统机械剪切方式进行马铃薯组培苗的无接触式切割,极大简化了组培苗移栽时的动作,能有效避免组培苗交叉感染,促进移栽的组培苗茎段成活。  相似文献   

低模数水玻璃对橡胶轻骨料混凝土力学性能的影响     

马快乐  王海龙  刘思盟  杨虹  张佳豪  王辉 《排灌机械工程学报》2021,39(7):685-691

为了了解不同掺量水玻璃对RLC(橡胶轻骨料混凝土)力学性能的影响,在RLC中加入水玻璃,并用20%粉煤灰代替水泥,制作水玻璃掺量分别为0,2%,4%,6%,8%的混凝土试块.通过核磁共振试验研究其微观孔隙变化及强度形成机理;进行对数函数曲线预测和BP神经网络强度预测,判断其可靠性并对比优劣.结果表明:水玻璃的掺入使RLC抗压强度呈现先增大后减小的趋势,其水玻璃最优掺量出现在2%;适量水玻璃的加入可提高80 目RLC力学性能,使其与最优组(20 目RLC组)28 d力学性能基本相同;水玻璃加入后混凝土孔隙度呈现出先减小后增大趋势,说明适量水玻璃的掺入可优化混凝土孔隙结构,提高混凝土强度;建立曲线拟合和BP-神经网络预测模型,2种预测模型都可用于混凝土龄期强度预测,但BP-神经网络预测模型的稳定性、可靠性及参数的全面性要优于曲线拟合模型.  相似文献