动物克隆的理论与实践     

李裕强  张涌 《西北农林科技大学学报(自然科学版)》2000,28(2):85-90

动物机体的大部分分化细胞具备全能性 ,成熟卵母细胞胞质中 m RNA、蛋白质等母型信息能使注入其中的细胞基因发生再程序化 ,从而启动新个体的发育。供体细胞和受体卵母细胞的周期和功能状态的同步协调是实现重构胚正常发育的关键。克隆动物的成功受供体细胞、受体卵母细胞以及二者之间的相互作用等多个因素的影响 ,其中的许多规律还未被掌握 ,动物克隆的研究必将揭示这些生物学规律 ,显示其巨大的科学意义  相似文献   

用美国白蛾核型多角体病毒表达家蚕促前胸腺激素基因   总被引：2，自引：0，他引：2  

贡成良  小林淳  金伟 《蚕业科学》2000,26(3):150-154

携带有家蚕促前胸腺激素PTTH基因的重组转移载体pHcMU1PTTH(含有麻蝇毒素IA信号序列 )与美国白蛾核型多角体病毒 (HcNPV)基因组DNA共转染SPIM细胞 ,经 2轮空斑纯化 ,筛选出重组病毒HcNPVPTTH+ ,SDSPAGE和Westernblot分析表明 :在细胞培养上清中可检测到PTTH的存在 ,说明麻蝇毒素IA信号序列可被SPIM细胞识别。用抗PTTH的兔血清进行Westernblot分析可显示分子量为2 0ku左右的 2条带 ,但当培养液内加入衣霉素时 ,仅出现 1条分子量小于 1 8ku的信号带 ,推测 2条信号带的产生与糖基化修饰不一致有关  相似文献   

Comparison of T-lymphocyte proliferation in canine epitheliotropic lymphosarcoma and benign lymphocytic dermatoses     

Marcia K. Murphy  & Thierry Olivry 《Veterinary dermatology》2000,11(2):99-105

The lesions of patch/plaque cutaneous T-cell lymphosarcoma (CTCL) are similar to many benign inflammatory dermatoses, both histopathologically and clinically. In humans, attempts to develop a simple, reliable immunophenotypic technique to differentiate between the malignant and benign dermatoses have been unsuccessful. The purpose of our study was to determine, using a proliferating cell nuclear antigen (PCNA)/CD3 double immunolabelling technique of paraffin-embedded sections, if the rate of T-cell proliferation in canine CTCL was greater than that of other diseases with non-neoplastic lymphocyte epitheliotropism. We selected cases of patch/plaque (PP) and tumour stage CTCL, erythema multiforme (EM) and cutaneous lupus erythematosus (CLE). We did not find a significant difference in the rate of T-cell proliferation in the epithelia nor the superficial dermis between PP-CTCL and EM. Whereas epithelial T-cell proliferation is significantly higher in PP-CTCL than CLE, it is not diagnostically useful because of overlap in the labelling indices.  相似文献   

OWE—SOJ技术及核迁移试验在香菇交配型因子鉴定中的应用   总被引：12，自引：1，他引：11  

林芳灿  汪中文 《华中农业大学学报》2000,19(6):573-576

由于没胡明确的形态差异,香菇的３种不亲和交配反应即Ａ＝Ｂ＝,Ａ＝Ｂ≠和Ａ≠Ｂ＝难以互相区别。本研究将ＯＷＥ－ＳＯＪ技术及核迁移试验用于香菇的交配型分析。结果ＯＷＥ－ＳＯＪ技术,可以观察到３种不同的菌落形态：由Ａ≠Ｂ≠配对形成的具锁状联合的绒毛状菌落,Ａ≠Ｂ＝配对形成的无锁状联合的栅栏型菌落及由Ａ＝Ｂ＝或Ａ＝Ｂ≠配对形成的无锁状联合的绒毛状菌落。通过一个附加的常规交配试验,Ａ＝Ｂ＝和Ａ＝Ｂ≠反应易于  相似文献   

EFFECTS OF A DIGITAL FILTER ON DETECTABILITY OF A PHANTOM LESION IN A SCINTIGRAM OF THE EQUINE TARSUS     

Per  Eksell  DVM  Sten  Carlsson  PhD  Peter  Lord  BVSc  FRCVS  Johan  Carlsten  DVM  PhD 《Veterinary radiology & ultrasound》2000,41(4):365-370

The aim of this study was to examine the effects of a digital filter on the detectability of a phantom lesion in a scintigram of the equine tarsus. Lateral images containing 50, 100, 150, 500, or 1000 kcounts were acquired. A created phantom lesion of 0 (normal), 10, 15, 20, or 30% increased intensity relative to local background was placed in the centrodistal tarsal joint area in the images of different count levels. Duplicate images were filtered with a Metz filter. The complete set of filtered and unfiltered images totaling 180 images was projected as slides to a group of 9 observers. The sensitivity in 50 and 100 kcount images was improved by applying the filter. Digital filtering increased the false positive fraction at all count levels but this effect was most pronounced in 500 and 1000 kcount images. Based on receiver operating characteristic analysis, filtering of a 50 kcount image improved image quality to that of a 150 kcount image.  相似文献   

影响哺乳动物细胞核移植效率的因素     

杨春荣  窦忠英 《中国牛业科学》2000,26(3):43-46

本文对哺乳动物胚胎细胞体细胞核移植克隆技术中显微操作的基本环节,以及细胞周期阶段对细胞核移植克隆胚胎效率影响的研究进行综述。  相似文献   

Soil organic matter transformations induced by Hieracium pilosella L. in tussock grassland of New Zealand     

H. Knicker  S. Saggar  R. Bäumler  P. D. McIntosh  I. Kögel-Knabner 《Biology and Fertility of Soils》2000,32(3):194-201

 To study the effect of Hieracium pilosella L. invasion on the transformations of soil organic matter of New Zealand tussock grassland soils (Ustochrepts), plant material and soils underneath Hieracium, the surrounding halo, and the adjacent herbfield (depleted tussock grassland) were examined for their chemical composition. An attempt was made to reveal possible changes in chemical composition of the soil organic matter induced by H. pilosella invasion. Small differences were detected by solid-state ¹³C nuclear magnetic resonance (NMR) spectroscopy in the composition of the plant and soil materials from these zones. Most of the differences in soil organic matter occurred due to differences in the amount and quality of plant-residue inputs. Comparable amounts of phenolic C were detected in the solid-state ¹³C NMR spectra of H. pilosella and herbfield vegetation, while alkaline CuO oxidation yielded considerable lower lignin oxidation products for H. pilosella. A slightly higher proportion of these compounds in H. pilosella soil revealed an accumulation and a low degradation rate of lignin compounds under H. pilosella. The HCl hydrolysis and solid-state ¹⁵N NMR spectroscopy showed similar chemical compositions of the N fractions of the three different soils. The absence of ¹⁵N NMR signal intensity assignable to aniline derivatives or aromatic heterocyclic N indicates that the condensation of phenolic compounds with N groups plays a minor role in N sequestration in these soils. Received: 6 September 1999  相似文献   

甘蓝夜蛾核型多角体病毒杀虫剂的制备及田间防治试验   总被引：10，自引：0，他引：10  

李长友  张履鸿  李国勋  郑桂玲  宋捷  许少甫  高洪祥  魏淳芳 《植物保护》2000,26(2):6-8

应用活体增殖病毒 ,制备甘蓝夜蛾核型多角体病毒杀虫剂乳剂 ,室内对 4龄甘蓝夜蛾幼虫的毒力测定 ,其LD₅₀为9.42×10³PIB/头。田间防治效果表明 ,采用1.0×10¹¹PIB/hm² 和3.0×10¹¹PIB/hm²的剂量防治甘蓝夜蛾的效果都在70%以上。乳液的稳定性较好 ,粘着性较强  相似文献   

金线莲组培苗的试管外生根和大田移栽技术   总被引：11，自引：0，他引：11  

何云芳  杨震 《浙江林业科技》1998,18(2):22-25

金线莲对生长环境要求严格。通过对组培苗试管外生根、“松口揭盖”“封口”炼苗及种植时期和基质选择等试验数据分析，总结了一套金线莲培苗的大田移栽技术，使移栽成活率高，植株生长健壮。  相似文献   

草鱼→鲤的细胞核移植     

齐福印  许桂珍  郑明霞 《中国兽医学报》1992,(1)

用草鱼的头肾组织细胞核、草鱼的头肾细胞核与鲤鱼成熟去核卵之杂种胚胎细胞核、草鱼的囊胚细胞核,分别与鲤鱼的去核卵细胞质配合进行核移植操作.结果,体细胞核移植,在亚科间的组合——草鱼核 鲤质中获得原肠期胚胎,得率为1%;体细胞继代核移植,在草鱼核 鲤质中获得胚孔封闭期胚胎,得率为5%;胚胎细胞核移植,在草鱼核 鲤质中获得眼球色素出现期胚胎,得率为2%.草鱼→鲤核-质杂种胚胎的胎盘形状、大小与鲤鱼的相似,而大于草鱼;胚盘细胞数量也多于草鱼胚胎同期胚盘,而类似于鲤鱼同期胚盘.  相似文献