糖对长白猪精液NAGaseⅡ的抑制机理     

林心宇  翁丽蓉  章文  黄小红 《兽医大学学报》2014,(11):1850-1854

以长白猪精液为材料,采用经典的纯化方法获得N-乙酰-β-D-氨基葡萄糖苷酶（EC 3.3.1.52,NAGase）的2种同工酶,命名为：NAGaseⅠ和NAGaseⅡ。其中NAGaseⅡ的比活力为358.21U/mg,纯化倍数为6.37倍。以海藻糖、D-甘露糖、蔗糖、葡萄糖为效应物,研究其对该酶活性的影响。结果表明：在特定浓度范围内,海藻糖对酶活力基本没有影响;D-甘露糖、蔗糖、葡萄糖对该酶均有一定的抑制作用;D-甘露糖对酶的抑制作用表现为非竞争型,抑制常数KI为11.94mmo/L;蔗糖对酶的抑制作用表现为竞争型,抑制常数KI为0.56mmol/L;葡萄糖对酶的抑制作用表现为混合型,抑制常数KI和KIS分别为0.35mol/L和64.29mmol/L。  相似文献   

白花蛇舌草多糖提取与纯化工艺研究进展     

陈志刚  刘湘新  瞿俊勇 《中兽医医药杂志》2014,(3):78-80

白花蛇舌草多糖具有抗肿瘤、抗菌等多种生物活性。目前,白花蛇舌草多糖的提取方法有超声波法、微波法、热水提取法等,此外,其纯化主要体现在脱蛋白、除色素等。笔者等就近年来国内的白花蛇舌草多糖提取与纯化的研究进展进行综述,并对其提取与纯化方法进行展望。  相似文献   

公路绿化带对机动车尾气污染的净化作用     

郭永晗  金鑫  杨鑫  蒲逸凡  高海波  胡增辉  沈应柏 《北京农学院学报》2014,29(4):78-81

选择车流量大,且绿化良好的北京西北四环六郎庄段为试验地,在早高峰时段采集距公路0,16,36 m处的空气样品,利用ATD-GC/MS（自动热脱附—气相色谱/质谱联用）技术对空气中的苯系物和氯代烷烃进行分析.结果表明,与没有绿化带的对照地相比,随着距离的增加空气中的苯系物和氯代烷烃的种类和含量降低的程度更为明显,而空气中萜烯化合物含量表现出相反的趋势,随距离增加迅速升高,说明绿化带植物通过吸收污染物,并释放萜烯等挥发物改变空气组成,对机动车尾气排放所造成的空气污染起到净化作用.  相似文献   

薏米对含铬污水的净化作用     

康亮  李素丽  李琼  李志刚  何冰  李正文  陈竑  陈伟刚  顾明华 《广西农业科学》2014,(1):63-66

[目的]研究铬胁迫下薏米对含铬污水的净化作用及铬胁迫对薏米生理生化的影响,为人工湿地净化含铬污水提供理论支持.[方法]开展桶栽模拟人工湿地试验,以薏米为材料,定期浇灌不同浓度的Cr（Ⅵ）生活污水（0、5、20、40、60mg/LK2Cr2O7）,定期测定进出水的化学需氧量（COD）和总磷（TP）,并检测薏米叶片过氧化物酶（POD）、超氧化物歧化酶（SOD）、丙二醛（MDA）等指标.[结果]薏米对含铬污水中COD的去除率随Cr（Ⅵ）浓度增大而降低,5mg/L的Cr（Ⅵ）处理在对总磷的去除率在90％以上,并随Cr（Ⅵ）浓度的升高而下降;薏米叶中的MDA含量随Cr（Ⅵ）浓度和处理时间的增加而呈增加趋势;叶片SOD、POD活性均随处理时间的延长而呈先上升后下降的趋势.[结论]薏米能耐受一定浓度的Cr（Ⅵ）胁迫,且对水质净化有较好的作用.  相似文献   

大孔吸附树脂纯化油茶壳中多酚类化合物的研究     

李嘉伟  邢鹏  张迎娴  陆英 《湖南农业科学》2014,(15)

以油茶壳为原料,采用大孔吸附树脂对油茶壳中多酚的分离纯化工艺条件进行了研究。考察了6种大孔吸附树脂对油茶壳中多酚类化合物的吸附分离性能,确定大孔树脂分离油茶壳中多酚类化合物的工艺条件。结果表明,AB-8树脂对油茶壳中多酚具有良好的吸附分离性能,其工艺条件为:质量浓度为3.78mg/mL,pH值3.7,油茶壳原料液以3BV/h的流速上柱吸附,再用6倍树脂体积的80%乙醇以3 BV/h的流速解吸,产品中多酚含量达61.3%,回收率为77.64%,样品得率为2.95%。  相似文献   

Glutathione and its Related Enzymes in the Nile Fish   总被引：2，自引：0，他引：2  

Ragaa R. Hamed  Tahany M. Maharem  Rasha A. M. Guinidi 《Fish physiology and biochemistry》2004,30(3-4):189-199

Glutathione (GSH) and related enzymes, glutathione transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR) form an important phase 2 biotransformation enzymes system. The objective of this study was to compare this enzymes system in three fish species from the river Nile, Oreochromis niloticus, Claris lazera and Cyprinus carpio in order to establish the main differences and to purify and characterize GST from the liver of O. niloticus.The level of GSH and the activity of GST, GPx and GR in the liver, kidney and gills of the three fish species were examined. A simple reproducible procedure for the purification of GST from the liver of O. niloticus to homogeneity, which includes chromatography on DEAE- cellulose followed by affinity chromatography on GSH-sepharose was established. The molecular mass was found to be 25,460 Da by SDS-PAGE. The Michaelis-Meneten constants (K_m) of the enzyme for GSH and CDNB were 0.35 mM and 0.42 mM, respectively. The affinity purified enzyme exhibited maximum pH at pH 8.0 and increasing pH above 8.0 did not affect the observed maximum. The purified enzyme acts readily on CDNB, less readily on some standard transferase substrates (1,2-dichloro-4-nitrobenzene and p-nitrophenethyl bromide) and not at all on others (bromosulphophthalein and p-nitrobenzyl chloride). Bromosulfophthalein, cibacron blue and hematin inhibited CDNB-conjugating activity of the purified enzyme with IC₅₀ 0.079, 3.98 and 0.126 μM, respectively.  相似文献   

鹿茸多肽提取分离纯化及药理作用研究进展     

陶荣珊  ;胡太超  ;李金伟  ;顾效瑜  ;王全凯 《经济动物学报》2014,(4):238-242

鹿茸多肽具有抗炎、抗氧化、增强免疫并能促进细胞分化、伤口愈合、抑制肿瘤细胞、促进神经系统、周围神经系统组织的再生等药理作用,是鹿茸中最有效的成分之一。文中对鹿茸多肽的提取,分离纯化及药理作用研究进展进行了综述,希望为鹿茸多肽的研究和开发提供参考。  相似文献   

大杯伞柄多糖与菌丝体粗多糖的免疫识别方法初探     

郑恒光  陈君琛  汤葆莎  吴俐  翁敏劼  高慧颖 《食用菌学报》2014,(3)

采用热水(80℃)提取和75%乙醇沉淀、Sepharose CL-4B凝胶过滤及DEAE Sepharose CL-4B阴离子交换等方法分离纯化大杯伞(Clitocybe maxima)柄粗多糖,获得伞柄多糖纯品,制备多糖抗体;采用热水法提取大杯伞菌丝体粗多糖。用碳二亚胺法将纯化伞柄多糖与牛血清白蛋白进行共价化学偶联,并将偶联产物(拟糖蛋白)免疫兔子以制备抗血清,以酶联免疫法检测抗体滴度。依据该抗体与来自相同菌株的菌丝体粗多糖免疫反应差异对这两种多糖进行免疫识别,结果表明:经过亲和层析纯化后,第六次免疫抗血清对伞柄多糖的抗体滴度可达64K,而且对菌丝体粗多糖反应呈阴性,显示该抗体可识别这两种多糖之间的结构差异。  相似文献   

Metallothionein induction in cultured fibroblasts and liver of a marine flatfish,the turbot,Scophthalmus maximus     

Stephen George  David Burgess  Michael Leaver  Nick Frerichs 《Fish physiology and biochemistry》1992,10(1):43-54

Intraperitoneal injection of turbot with Cd induced the synthesis of a low molecular weight hepatic Cd-binding protein and a 500bp mRNA, which hybridised to a plaice metallothionein (MT) cRNA probe. The Cd-binding protein displayed cross-reactivity in a competitive ELISA with antiserum raised against rainbow trout MT and had the characteristic amino acid composition, metal stoichiometry and spectral characteristics of a Class I MT. Only one isoform was apparent on ion exchange chromatography. Southern blot analysis of DNA cleaved with four restriction enzymes suggested that only a single MT gene is present in turbot.In an established turbot fibroblast cell line, Cd induced MT mRNA and MT levels in a dose and time-dependent manner. MT was also induced by Cu, Hg and Zn but not Pb exposure. Physiological concentrations of glucocorticoids and sex hormones did not induce MT synthesis, although at high concentrations a positive response to corticosterone, dexamethasone, hydrocortisone or progesterone was observedin vitro indicating the possible presence of a functional steroid regulatory element in the fish MT gene.Abbreviations used AMP adenosine monophosphate - CHSE chinook salmon embryo - DMSO dimethyl sulphoxide - HPLC high performance liquid chromatography - KB kenacid blue - MT metallothionein - Mr molecular weight (kDaltons) - NR neutral red - PEG polyethylene glycol - RTH rainbow trout hepatoma - SDS sodium dodecyl sulphate - SSC 0.15M NaCl, 0.015M sodium citrate - TF turbot fibroblast  相似文献   

The purification and partial characterization of carp,Cyprinus carpio,vitellogenin   总被引：2，自引：0，他引：2  

Charles R. Tyler  John P. Sumpter 《Fish physiology and biochemistry》1990,8(2):111-120

A procedure is described for the isolation of intact vitellogenin (c-VTG) from the carp, Cyprinus carpio. VTG was induced in juvenile females using oestradiol-17β and purified from the plasma using a combination of gel-filtration chromatography on Sepharose 6B and ion exchange chromatography on DEAE-cellulose. Purification procedures were conducted at low temperatures (below 9°C) in the presence of the proteolytic enzyme inhibitor aprotinin to prevent degradation. Intact c-VTG had an apparent molecular mass of 390,000 Daltons, but when extracted from plasma in the absence of aprotinin it underwent proteolysis into at least 2 protein fragments (apparent molecular masses of 230,000 and 96,000 Daltons), showing an instability of the native dimer. An amino acid analysis of c-VTG showed that its composition was almost identical to goldfish VTG, a species closely allied to the true carps and also similar to other oviparous vertebrate VTGs. Collectively, these data indicate that using these purification procedures VTG from carp, and probably other teleost species, can be isolated in an intact, highly purified form.  相似文献