中华蜜蜂精液的冷冻贮存技术(英文)     

王丽华  张其康  侯光珊  罗建能  娄鲲鹏  于文俊 《福建农林大学学报(自然科学版)》1999,28(2)

中华蜜蜂精液在液氮（－ １９６ ℃） 中贮存１２１ ｄ,精液与含有８％ 二甲亚砜的三羟基氨基甲烷缓冲稀释液以１∶１．５ 混合,不用平衡,在广口保温瓶中距液氮面２ ｃｍ 或５ ｃｍ 处预冻,在３５～３９ ℃水浴中解冻,可产生较好的解冻后活力比率（超过６０％ ）．当室温高于２０ ℃时（４ 月份）,样本必须于１３ ℃下平衡０．５ ｈ 左右,其解冻后活力比率可达３０％ ．春季采集并冷冻的样本,其解冻后活力不如冬季采集并冷冻的．用在液氮中贮存了１～１２１ ｄ 的精液分别以 ４～５ μ Ｌ的剂量给 ２４ 只处女王人工授精,４ 只开产但首先产的不是受精卵．解冻后的精液能够于１３ ℃下保存４６ ｈ．磺胺不能用于精子冷冻．  相似文献   

鸸鹋人工采精     

孙得发  魏忠义 《中国家禽》1999,21(10):5-6

本文探讨了以人为假台畜引导公鸸鹋产生性冲动,用一次性软塑料杯人工徒手采精。并对精液量和精液品质进行了测定,鸸鹋精液量为0．4-1．4mL,pH为7.4-7.7,精子密度极高,精液为乳白色。试验表明,鸸鹋的采精量与季节有关。同时提出鸸鹋人工采精的一些注意事项。  相似文献   

Sperm cryopreservation of tiete tetra Brycon insignis (Characiformes): effects of cryoprotectants,extenders, thawing temperatures and activating agents on motility features     

Ana T M Viveiros  Thiciana B Amaral  Laura H Orfão  Ziara A Isaú  Danilo Caneppele  Marcelo C Leal 《Aquaculture Research》2011,42(6):858-865

The aim of this study was to test the effects of cryoprotectants [dimethyl sulphoxide (DMSO) and methylglycol], extenders (0.9% NaCl, 5% glucose, Beltsville Thawing Solution^? and Merck III^?), thawing temperatures (30 and 60 °C) and activating agents (0.29% NaCl and 1% NaHCO₃) on the cryopreservation process of tiete tetra Brycon insignis sperm. Sperm was loaded in 0.5 mL straws, frozen in nitrogen vapour at ?170 °C and stored in liquid nitrogen. Post‐thaw sperm quality was evaluated in terms of subjective motility rate, quality motility score (0=no movement; 5=rapidly swimming spermatozoa), duration of motility and vitality (eosin–nigrosin staining). Post‐thaw sperm motility rate was greater in methylglycol (76–88%), compared with DMSO (23–59%). In general, the highest quality motility scores were observed when sperm was thawed at 30 °C and triggered in 1% NaHCO₃ (3.5–4.3). Duration of motility was longer when triggered in 1% NaHCO₃ (95–120 s) compared with 0.29% NaCl (69–107 s). Sperm vitality was not affected by any of the parameters tested and varied from 51% to 69% intact sperm. Brycon insignis sperm frozen in methylglycol combined with any of the extenders tested and using the methods described above yields motility above 57% and that should last long enough to fertilize oocytes.  相似文献   

圆口铜鱼精浆的理化性质分析     

王昌霖  吴兴兵  朱永久  杨德国  王亚龙  陈亮  杨少荣  王小明 《淡水渔业》2017,47(3):41-44

对圆口铜鱼(Coreius guichenoti)精浆离子和氨基酸成分及精液生理特性进行了检测分析。结果显示,圆口铜鱼精液pH值为7.3,呈弱碱性;精液浓度为39.7%,精子密度为5.3×10~9个/mL;精浆离子以Na~+含量88.7 mmol/L最高,其次是K~+,之后为Ca~(2+)、Mg~(2+)、Zn~(2+)、Cu~(2+);精浆水解氨基酸总量为2 872.69μmol/100mL,其中以脯氨酸含量最高,蛋氨酸、酪氨酸和组氨酸含量最低。该结果填补了圆口铜鱼繁殖生物学的相关数据,为圆口铜鱼规模化人工繁育提供了基础资料。  相似文献   

Dietary profile of n‐3 series LC‐PUFAs in rainbow trout under regular stripping condition: Semen production and quality,hepato‐somatic index,haemato‐immunologic values,oxidative stress and fatty acid composition of liver,muscle and semen     

Serpil Mie Yonar  Kenan Kprücü  Sinan zcan 《Aquaculture Research》2020,51(1):370-378

In this study, the semen production and quality, hepato‐somatic index, haemato‐immunologic values, oxidative stress and the fatty acid contents in liver, muscle and semen of rainbow trout fed diets supplemented n‐3 series long‐chain polyunsaturated fatty acids (LC‐PUFAs) under regular stripping condition were investigated. For this aim, three diets (Control, D1 and D2) were prepared. These diets were contained n‐3 LC‐PUFAs (as a percentage of dietary total fatty acid) at 3.14%, 7.84% and 13.63% respectively. Experimental fish were fed with the control and test diets. The highest hepato‐somatic index, spermatologic (semen pH and volume, sperm motility and density), haematologic (haematocrit value, haemoglobin, erythrocyte count, corpuscular volume, haemoglobin and its concentration in corpuscular), immunologic (nitroblue tetrazolium activity, leucocyte count, phagocytic index, protein and immunoglobulin [IgM] in total plasma) and antioxidants (reduced glutathione, catalase, glutathione peroxidase and superoxide dismutase) values were found in fish fed the D2, D1 and control diets respectively (p < 0.01). Increase in the dietary n‐3 LC‐PUFAs was not significantly (p > 0.01) increased the oxidative stress (malondialdehyde) in fish. The results indicated that the n‐3 LC‐PUFAs at 13.63% level of total fatty acid in the diet could increase the semen production and quality, hepato‐somatic index, haematologic and immunologic values, and the n‐3 LC‐PUFA contents in liver, muscle and semen of rainbow trout broodstock under regular stripping condition.  相似文献   

Effect of ascorbic acid supplementation on zootechnical performance,haematological parameters and sperm quality of lebranche mullet Mugil liza     

Jorgelia Castro  Caio Magnotti  Morgana Angelo  Fabio Sterzelecki  Fabiola Pedrotti  Maria Fernanda Oliveira  Thiago Soligo  Dbora Fracalossi  Vinicius Ronzani Cerqueira 《Aquaculture Research》2019,50(11):3267-3274

The feed provided to breeding fish is one of the main factors influencing the quality of fish gametes. This study was carried out to evaluate the influence of ascorbic acid on growth, haematological parameters and sperm quality of Lebranche mullet males (Mugil liza). Six diets with different levels of ascorbic acid (0; 53; 107; 216; 482 and 708 mg/kg) were tested in triplicate for 75 days. During spermiation (first gonadal maturation), 144 individuals (205.7 ± 11.5 g and 25.7 ± 0.4 cm) were randomly distributed in 18 experimental tanks. Growth parameters were evaluated at the beginning and end of the experiment. Fish blood was collected to analyse glucose, total protein and erythrocyte count (EC) (n = 9). Fish (n = 12) from each treatment were euthanized to determine hepatosomatic index (HSI) and gonadosomatic index (GSI). Semen was collected to evaluate spermatic density, cell membrane integrity and sperm motility. No difference (p > .05) was found on growth parameters, GSI, HSI and total protein. However, EC was lower in fish fed without ascorbic acid (the control group). Ascorbic acid supplementation provided positive effects on sperm characteristics. Fish from treatments with 53 and 107 mg/kg presented the best results for motility time (133.30 ± 4.25 and 135.00 ± 2.77 respectively). Treatments with 107, 216 and 708 mg/kg provided the best results for motility rate (92.0 ± 2.9%, 93.0 ± 5.8% and 93.0 ± 5.8% respectively). Supplementation with 107 and 216 mg/kg provided the best results for plasma membrane integrity (70.12 ± 9.10% and 76.3 ± 3.1% respectively). Lower spermatic density was observed in fish without ascorbic acid supplementation, although no difference was found in sperm density among the treatments with ascorbic acid (p < .05). Considering these results, supplementation of dietary ascorbic acid between 107 and 216 mg/kg optimizes the spermatic quality in males of lebranche mullet.  相似文献   

甘肃高寒牧区藏羊电刺激采精实验     

孙万林  王福财  王淑芳  白天俊 《畜牧兽医杂志》2023,(3):13-16

为研究电刺激采精技术能否在甘肃高寒牧区藏系羊繁殖中推广应用,该技术是否对公羊精液品质和采精量有影响,项目课题组在甘肃省天祝县旦马乡进行了牧区藏羊种公羊电刺激采精实验。选择本土选育的欧拉型藏羊种公羊,配种期每日每只采精2次,即：首日（标注为d1）早上采用传统的假阴道采精法采精,下午采用电刺激法采精,次日（标注为d2）早上采用电刺激法采精,下午采用传统的假阴道采精法采精,依次不同采精方法交叉采精,减少误差,连续采精6d,及时检查采精量及精液密度、精子活力等品质。实验结果表明,甘肃天祝牧区藏羊种公羊电刺激采精所得精液量均在0.80 mL～0.96 mL之间,符合藏系羊正常精液量,平均精子活力均在0.80以上,平均精液密度均“中”以上,精液品质符合人工授精精液品质要求,可应用于藏羊繁殖生产实践。  相似文献   

第八师奶牛养殖产业现状及政策建议     

王丹  王煜舒  杨阳  孙敬礼 《畜牧兽医杂志》2023,(2):24-26

八师奶牛养殖优势突出,已形成以奶牛养殖为基础、乳制品加工为支撑的“养-加-销”于一体的产业链条,掌握师市规模化奶牛场的生产、管理和发展现状。通过加强政策支持和技术服务支撑,提高繁育水平,降低饲养成本。助力师市奶牛养殖扩繁增量突破10万头的目标。  相似文献   

不同浓度大豆卵磷脂替代蛋黄对东佛里生奶绵羊精液冷冻保存效果的影响     

赵霞  爱伦高娃  马跃军  樊东  乌仁图亚  娜仁花  吉林台 《畜牧兽医杂志》2023,(3):95-98

本试验皆在研究添加不同浓度大豆卵磷脂（SL）冷冻保存东佛里生奶绵羊精液的效果。我们在Tris基础稀释液中,添加18%蛋黄为对照组,添加0.5%、1%、1.5%、2%、2.5%SL设为试验组,检测冷冻精液解冻后的精子活率和顶体完整率。结果显示,添加0.5%、2.5% SL冷冻稀释液稀释的精液,解冻后精子活率和顶体完整率与其他组之间存在显著差异（P<0.05）;添加18%蛋黄和1%～2% SL冷冻稀释液稀释的精液,冷冻解冻后精子活率和顶体完整率之间无显著差异（P>0.05）;添加18%蛋黄和1.0%～1.5% SL冷冻稀释液稀释后的精液,进行人工授精后母羊的妊娠率与对照组无显著差异（P>0.05）。因此,大豆卵磷脂可以作为冷冻保护剂用于东佛里生奶绵羊精液的冷冻保存,其最佳添加浓度为1～2%（g／L）。  相似文献   

藏羊电刺激采精和人工授精技术     

郭玉鑫  白天俊  王淑芳  王福财  王占斌  孙万林 《畜牧兽医杂志》2023,(2):87-90

由于藏羊特殊的品种特性,高海拔严寒地区养殖适应性好,养殖成本相对其他类型羊较低,但自然交配情况下,空怀率高,繁殖率低。为克服藏种羊胆小易惊,调教不易,采精困难等不利因素,我们采用电刺激法促使种公羊被动排精,很大程度上降低了采精难度,提高了采精数量,在母羊集中发情季节,优秀种公羊个体可授配母羊500只以上,同时引进药物诱导母羊同期发情等技术进行人工授精,大大提升了藏羊繁殖性能,加速了我县藏羊引种改良进程,提高藏羊养殖经济效益。  相似文献