Idiopathic eosinophilic colitis lesions of the equine small (descending) colon          下载免费PDF全文

M. P. de Bont  A. J. Malbon  R. Blundell  D. C. Archer 《Equine Veterinary Education》2018,30(9):466-471

Five horses with a primary surgical lesion of the small (descending) colon were diagnosed with eosinophilic colitis based on visual and histopathological examination. These were evident as visibly striking, hyperaemic, focal lesions of the small colon, with serosal petechiation, oedema and marked thickening of the intestinal wall at the site. Areas of focal necrosis were also evident. The gross appearance of the lesions were considered to be sufficiently severe in all cases to merit resection, due to concerns about intestinal necrosis and septic peritonitis. An inability to fully exteriorise the affected portion of intestine to perform a resection and anastomosis necessitated intraoperative euthanasia of one horse. A total of three horses survived to hospital discharge. Eosinophilic colitis lesions are a rare cause of severe small colon disease, but should be considered in cases with similar visual characteristics.  相似文献   

Proton accumulation accelerated by heavy chemical nitrogen fertilization and its long-term impact on acidifying rate in a typical arable soil in the Huang-Huai-Hai Plain     

HUANG Ping  ZHANG Jia-bao  XIN Xiu-li  ZHU An-ning  ZHANG Cong-zhi  MA Dong-hao  ZHU Qiang-gen  YANG Shan  WU Sheng-jun 《中国农业科学(英文版)》2015,(1)

Cropland productivity has been signiifcantly impacted by soil acidiifcation resulted from nitrogen (N) fertilization, especialy as a result of excess ammoniacal N input. With decades’ intensive agricul...  相似文献   

患红体病凡纳滨对虾肝胰腺NAGase基本性质的改变研究     

谢晓兰  龚丽芬  魏晓倩  黄乾生  陈清西 《安徽农业科学》2011,39(21):12906-12908

[目的]为虾养殖过程中红体病的诊断和控制提供重要的理论依据。[方法]分别测定健康和患红体病的凡纳滨对虾（Litopenaeus vanname）的肝胰腺N-乙酰-β-D-氨基葡萄糖苷酶（EC3.2.1.52,NAGase）的比活力及其性质差异。[结果]2种来源NAGase的比活力、基本酶学性质等均存在差异。对虾患红体病后,肝胰腺NAGase的比活力和最适温度下降,但催化反应动力学常数Km、Vm值和活化能上升。[结论]该研究表明NAGase与对虾患病可能存在着密切关系。  相似文献   

7株根瘤菌菌株与热研2号柱花草共生最适磷浓度研究     

董荣书  张洁  黄艳霞  白昌军  郇恒福  王文强  刘国道 《热带作物学报》2014,35(5):832-836

采用水培的方法,设3个磷水平,7个菌株,通过对株高、茎叶鲜重、茎叶干重、固氮酶活性、茎叶氮含量、茎叶磷含量的测定及综合分析,确定使热研2号柱花草接种不同根瘤菌菌株达到最佳促生性能的磷浓度。结果表明:7个菌株在中磷时鲜重和含氮量最大,低磷不利于鲜重和含氮量的增加;经主因子综合分析,菌株YM11-1、RJS9-2、PN13-3在高磷时对热研2号柱花草有最好的固氮促生效果,菌株LZ3-2、BS1-1、PN13-3在中等磷条件下有最好的固氮促生效果,菌株FS3-1-1在高磷和中磷都有最好的固氮促生效果。  相似文献   

Volatile profile,fatty acids composition and total phenolics content of brewers' spent grain by-product with potential use in the development of new functional foods     

《Journal of Cereal Science》2015

Brewers' spent grain (BSG) is the insoluble residue generated from the production of wort in the brewing industry. This plant-derived by-product is known to contain significant amounts of valuable components, which remain unexploited in the brewing processes. Therefore, it is essential to develop a more detailed characterization of BSG in order to highlight its potential in developing new value-added products and simultaneously solve the environmental problems related to its discharge. The content of BSG in several biologically active compounds (fatty acids, polyphenols, flavonoids, antioxidant capacity) as well as its volatile fingerprint were assessed and compared with the composition of barley, malt and wheat flour samples. The obtained results emphasized the importance and the opportunities of the re-use of this agro-industrial by-product.  相似文献   

Rapid detection of contagious ecthyma by loop-mediated isothermal amplification and epidemiology in Jilin Province China     

Kai WANG  Hongze SHAO  Zhihua PEI  Guixue HU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):125-128

The aim of this experiment was to develop a loop-mediated isothermal amplification (LAMP) assay and to research the recent epidemiology of contagious ecthyma in Jilin Province, China, using the assay. A LAMP assay targeting a highly conserved region of the F1L gene was developed to detect contagious ecthyma virus (CEV). Three hundred and sixty-five cases from 64 flocks in 9 different areas of Jilin Province, China, from 2011 to 2014 were tested using the LAMP assay. The results showed that the sensitivity of the LAMP assay was 100 copies of the standard plasmid, which is 100-fold higher than the sensitivity of PCR. No cross-reactivity was observed with capripoxvirus, fowlpox virus, foot-and-mouth disease virus serotype O, foot-and-mouth disease virus serotype Asia I and bluetongue virus. The average positive rate was 19.73% (72/365), and the positive rate was highest in lambs aged 1–6 months. Our results demonstrated that CEV infection was very widespread in the flocks of Jilin Province and that the LAMP assay allows for easy, rapid, accurate and sensitive detection of CEV infection.  相似文献   

安徽省珍稀植物新纪录     

张中信 《现代农业科技》2014,(24)

水禾属和水禾为安徽省植物分布新纪录属和种。该文介绍了采自安徽省无为县禾本科单种属珍稀植物水禾的主要形态特征、生态特性和繁殖方法。  相似文献   

核磁共振法测定甲哌鎓原药的含量     

魏泱  王晓莉  郭幸丽  刘晶晶 《农药科学与管理》2014,35(12):49-52

采用定量核磁共振法建立了甲哌鎓原药的定量方法。以重水为溶剂,仲丁基脲为内标物,以化学位移分别在δ3.12的甲哌鎓甲基和δ1.10的仲丁基脲甲基的1H-NMR信号作为定量峰。以甲哌鎓与仲丁基脲定量峰的峰面积比(AS/AI)对甲哌鎓与仲丁基脲的质量比(WS/WI)进行线性回归,线性相关系数为0.999 9;重复测定同一批次原药含量,变异系数为0.28%。采用核磁共振波谱法对甲哌鎓进行定量分析,操作简便,测定速度快,准确度高。  相似文献   

miR-122靶向调控鸡BZW2基因的表达     

李艳艳  王星果  郁建锋  邵芳  张燕萍  顾志良 《农业生物技术学报》2016,(11):1734-1740

miR-122(MicroRNA-122)是在肝脏中高表达的miRNA,在肝脏的生长发育和脂类代谢等过程发挥重要作用,BZW2 (basic leucine zipper and W2 domains 2)主要参与蛋白质合成代谢,本研究目的是确定BZW2是否为miR-122调控的靶基因.通过生物信息学预测miR-122的靶基因,并分析BZW2的3-UTR区域与miR-122种子区的配对情况,再用双荧光素酶报告系统和突变实验证明miR-122作用于BZW2的3'UTR.用荧光定量PCR检测转染miRNA-122mimic的鸡(Gallus gallus)肝癌细胞系(leghorn hepatocellar,LMH)细胞和转染LNA-antimiR-122的鸡原代肝细胞中BZW2的表达.鸡BZW2的3'-UTR区域与miR-122种子区互补配对,荧光素酶报告基因和突变实验分析表明,miR-122能够通过与BZW23'-UTR区结合抑制基因的表达;将miR-122在LMH细胞中过表达后,发现BZW2 mRNA表达水平显著下降;利用LNA-antimiR-122抑制鸡肝脏原代细胞中的miR-122后,BZW2 mRNA表达水平呈显著性上升.结果证明BZW2是miR-122的靶基因,其在mRNA水平上受到miR-122的负性调控,本研究为揭示miR-122在鸡肝脏中的广泛的功能提供了理论依据.  相似文献   

Environmental performances of giant reed (Arundo donax L.) cultivated in fertile and marginal lands: A case study in the Mediterranean     

《European Journal of Agronomy》2016

Perennial rhizomatous grasses (PRGs) tend to have a high yield combined with a low environmental impact. Cultivation in marginal or poorly cultivated land is recommended in order not to compromise food security and to overcome land use controversies. However, the environmental impacts of using different types of soil are still unclear. We thus assessed the environmental impact of two giant reed (GR) systems cultivated in a fertile soil (FS) and in a marginal soil (MS) through a cradle-to-plant gate LCA. We analyzed energy balance, GHG emissions (including LUC, not including iLUC), and the main impacts on air, water and soil quality. In both systems the annualized soil carbon sequestration was more than twofold the total GHG emitted, equal to −6464 kg CO₂eq ha⁻¹ in FS and −5757 kg CO₂eq ha⁻¹ in MS. Overall, soil characteristics affected not only GR yield level, but also its environmental impact, which seems to be higher in the MS system both on a hectare and tonne basis. The production of GR biomass in marginal soil could thus lead to higher environmental impacts and a more extensive land requirement.  相似文献